BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= tesS0041 (548 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 136 3e-33 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 91 1e-19 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 90 2e-19 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 70 3e-13 SPBC12C2.10c |pst1|SPBC21D10.01c|Clr6 histone deacetylase comple... 29 0.45 SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 28 0.79 SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 2.4 SPBC428.20c |alp6|SPBC902.01c|gamma tubulin complex Spc98/GCP3 s... 26 3.2 SPAC27D7.10c |||But2 family protein|Schizosaccharomyces pombe|ch... 26 4.2 SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 26 4.2 SPBC1105.04c |cbp1|abp1|CENP-B homolog|Schizosaccharomyces pombe... 26 4.2 SPAC27D7.09c |||But2 family protein|Schizosaccharomyces pombe|ch... 26 4.2 SPAPB24D3.02c |||amino acid permease, unknown 3|Schizosaccharomy... 25 5.6 SPAC24C9.14 |otu1|mug141|ubiquitin-specific protease |Schizosacc... 25 5.6 SPAC1834.02 |aro1||pentafunctional aromatic polypeptide Aro1 |Sc... 25 9.7 SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 25 9.7 SPAC7D4.12c |||DUF1212 family protein|Schizosaccharomyces pombe|... 25 9.7 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 136 bits (328), Expect = 3e-33 Identities = 59/78 (75%), Positives = 68/78 (87%) Frame = +3 Query: 237 GQVRPAAVMVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSV 416 G+ P AV+VDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+V Sbjct: 57 GKYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAV 116 Query: 417 LDVVRKEAEGCDCLQGFQ 470 LDVVR+EAE CD LQGFQ Sbjct: 117 LDVVRREAEACDALQGFQ 134 Score = 109 bits (262), Expect = 3e-25 Identities = 49/74 (66%), Positives = 58/74 (78%) Frame = +1 Query: 70 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDATGAYSGDSDLQLERINVYYNEASGGKYV 249 MREIVHIQAGQCGNQ+GA FW I+DEHG+D+ G Y G S+ Q ER+NVY+NEA+GGKYV Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60 Query: 250 PRL*WWTLSLEPWT 291 PR + LEP T Sbjct: 61 PRA--VLVDLEPGT 72 Score = 29.5 bits (63), Expect = 0.34 Identities = 12/13 (92%), Positives = 13/13 (100%) Frame = +2 Query: 509 GTLLISKIREEYP 547 GTLL+SKIREEYP Sbjct: 148 GTLLLSKIREEYP 160 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 91.1 bits (216), Expect = 1e-19 Identities = 37/78 (47%), Positives = 55/78 (70%) Frame = +3 Query: 234 RGQVRPAAVMVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDS 413 +G+ P ++ VDLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++DS Sbjct: 62 QGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDS 121 Query: 414 VLDVVRKEAEGCDCLQGF 467 VL+ +R+ A+ C LQGF Sbjct: 122 VLERIRRMADNCSGLQGF 139 Score = 45.6 bits (103), Expect = 5e-06 Identities = 23/68 (33%), Positives = 36/68 (52%), Gaps = 6/68 (8%) Frame = +1 Query: 70 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDATGAYSGDSDLQL------ERINVYYNEA 231 MRE++ + GQ G QIG WE+ EHGI G + +S++ + +++E Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60 Query: 232 SGGKYVPR 255 GK+VPR Sbjct: 61 GQGKFVPR 68 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 89.8 bits (213), Expect = 2e-19 Identities = 37/78 (47%), Positives = 53/78 (67%) Frame = +3 Query: 234 RGQVRPAAVMVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDS 413 +G+ P ++ VDLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD Sbjct: 58 QGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDE 117 Query: 414 VLDVVRKEAEGCDCLQGF 467 V D +R+ A+ C LQGF Sbjct: 118 VTDKIRRIADNCSGLQGF 135 Score = 51.6 bits (118), Expect = 7e-08 Identities = 26/64 (40%), Positives = 35/64 (54%), Gaps = 2/64 (3%) Frame = +1 Query: 70 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDATGAYSGDSDLQLE--RINVYYNEASGGK 243 MREI+ I GQ G QIG WE+ EHGI G + ++ Q + +++E GK Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60 Query: 244 YVPR 255 YVPR Sbjct: 61 YVPR 64 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 69.7 bits (163), Expect = 3e-13 Identities = 27/75 (36%), Positives = 51/75 (68%), Gaps = 2/75 (2%) Frame = +3 Query: 249 PAAVMVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLD 422 P A+++DLEP ++++ S +G ++ P+N + ++ GAGNNWA G Y+ + + ++D Sbjct: 62 PRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMD 120 Query: 423 VVRKEAEGCDCLQGF 467 ++ +EA+G D L+GF Sbjct: 121 MIDREADGSDSLEGF 135 Score = 62.1 bits (144), Expect = 5e-11 Identities = 25/61 (40%), Positives = 41/61 (67%) Frame = +1 Query: 73 REIVHIQAGQCGNQIGAKFWEVISDEHGIDATGAYSGDSDLQLERINVYYNEASGGKYVP 252 REI+ +QAGQCGNQIG++FW+ + EHGI G + ++R +V++ ++ +Y+P Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62 Query: 253 R 255 R Sbjct: 63 R 63 >SPBC12C2.10c |pst1|SPBC21D10.01c|Clr6 histone deacetylase complex subunit Pst1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1522 Score = 29.1 bits (62), Expect = 0.45 Identities = 18/52 (34%), Positives = 22/52 (42%) Frame = -3 Query: 513 VPYQIQYLLPENELAENPADNRSLLLLF*LHPVPNQPVQPPLCNVLSPSYSP 358 + Y YL P +A +P N L H P P PL N +SP Y P Sbjct: 24 INYTGPYLTPSGTMAYHPG-NAPLFTQAPPHTNPQGPPPFPLFNSISPVYDP 74 >SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 255 Score = 28.3 bits (60), Expect = 0.79 Identities = 14/32 (43%), Positives = 17/32 (53%) Frame = -2 Query: 361 PAPDCPKTKLSGRKICPNGPERTESMVPGSRS 266 P+ PK L R I PNGPE + + GS S Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51 >SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizosaccharomyces pombe|chr 1|||Manual Length = 506 Score = 26.6 bits (56), Expect = 2.4 Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%) Frame = +3 Query: 303 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 404 GP+G +F P F+F +G NW+ Y E A L Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190 >SPBC428.20c |alp6|SPBC902.01c|gamma tubulin complex Spc98/GCP3 subunit Alp6|Schizosaccharomyces pombe|chr 2|||Manual Length = 821 Score = 26.2 bits (55), Expect = 3.2 Identities = 10/35 (28%), Positives = 18/35 (51%) Frame = -3 Query: 138 YFPELCSDLIPALTRLDVDDFPHDCTKNLEPPESN 34 +F E+ + P +LD+DD + + P E+N Sbjct: 36 FFQEIIHSISPDTFQLDIDDILYKIYSKIPPEENN 70 >SPAC27D7.10c |||But2 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 383 Score = 25.8 bits (54), Expect = 4.2 Identities = 17/53 (32%), Positives = 26/53 (49%) Frame = +2 Query: 35 FDSGGSKFLVQS*GKSSTSRRVNAGIRSEQSSGK*SRTSTALTPRVLTAATPT 193 F S S S +STS RV++ ++ SSG + T+ + V +AT T Sbjct: 165 FVSPSSSSSSSSSAATSTSTRVSSSAKASTSSGAIAYTTKCVVVPVTASATAT 217 >SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual Length = 234 Score = 25.8 bits (54), Expect = 4.2 Identities = 12/30 (40%), Positives = 18/30 (60%) Frame = +3 Query: 372 AKGHYTEGAELVDSVLDVVRKEAEGCDCLQ 461 A+GH G ELV + D +RK++E L+ Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212 >SPBC1105.04c |cbp1|abp1|CENP-B homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 522 Score = 25.8 bits (54), Expect = 4.2 Identities = 9/21 (42%), Positives = 15/21 (71%) Frame = -3 Query: 501 IQYLLPENELAENPADNRSLL 439 +QY+L +NEL NP + ++L Sbjct: 342 VQYILEQNELGRNPYNTTNVL 362 >SPAC27D7.09c |||But2 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 383 Score = 25.8 bits (54), Expect = 4.2 Identities = 17/53 (32%), Positives = 26/53 (49%) Frame = +2 Query: 35 FDSGGSKFLVQS*GKSSTSRRVNAGIRSEQSSGK*SRTSTALTPRVLTAATPT 193 F S S S +STS RV++ ++ SSG + T+ + V +AT T Sbjct: 165 FVSPSSSSSSSSSAATSTSTRVSSSAKASTSSGAIAYTTKCVVVPVTASATAT 217 >SPAPB24D3.02c |||amino acid permease, unknown 3|Schizosaccharomyces pombe|chr 1|||Manual Length = 543 Score = 25.4 bits (53), Expect = 5.6 Identities = 7/21 (33%), Positives = 14/21 (66%) Frame = -1 Query: 95 AWMWTISLMIALKIWNPPNRI 33 AW+W ++LM+ + PN++ Sbjct: 458 AWLWFMALMLLFPSYQNPNKV 478 >SPAC24C9.14 |otu1|mug141|ubiquitin-specific protease |Schizosaccharomyces pombe|chr 1|||Manual Length = 329 Score = 25.4 bits (53), Expect = 5.6 Identities = 9/21 (42%), Positives = 13/21 (61%) Frame = -3 Query: 411 NQPVQPPLCNVLSPSYSPPQT 349 N+P +PP+ N + PPQT Sbjct: 86 NEPAKPPIPNAATKPTFPPQT 106 >SPAC1834.02 |aro1||pentafunctional aromatic polypeptide Aro1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1573 Score = 24.6 bits (51), Expect = 9.7 Identities = 14/48 (29%), Positives = 20/48 (41%) Frame = +1 Query: 49 FQIFSAIMREIVHIQAGQCGNQIGAKFWEVISDEHGIDATGAYSGDSD 192 F + S I I C + +W+V+ G+ TGA S SD Sbjct: 807 FSVLSLICPSRTLIIDKACVEKTWPYWWDVLHQSFGVKLTGATSVASD 854 >SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1275 Score = 24.6 bits (51), Expect = 9.7 Identities = 14/56 (25%), Positives = 25/56 (44%) Frame = -1 Query: 458 QTIAAFCFFSNYIQYRINQFSPLCVMSFRPVIPRPRLSEDEVVRSEDLPKRPGTDG 291 Q AA+ +S++ + FSP + P + P ++ V LP+ P + G Sbjct: 109 QNSAAYTTYSSFPNALFDDFSPNNPLDTDPFLTSPGNKQNTVDSFRPLPETPVSPG 164 >SPAC7D4.12c |||DUF1212 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 759 Score = 24.6 bits (51), Expect = 9.7 Identities = 9/25 (36%), Positives = 14/25 (56%) Frame = -1 Query: 326 SEDLPKRPGTDGVHGSRLKVHHHSR 252 +E++P+ T G + HHHSR Sbjct: 283 TEEIPQFSKTGHSRGRKFNFHHHSR 307 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,307,543 Number of Sequences: 5004 Number of extensions: 46553 Number of successful extensions: 173 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 155 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 170 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 227943826 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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