BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= tesS0015 (598 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 156 2e-39 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 99 6e-22 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 98 1e-21 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 78 1e-15 SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 29 0.52 SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 28 0.90 SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 28 0.90 SPBC106.09 |cut4|apc1|anaphase-promoting complex subunit Apc1|Sc... 27 1.6 SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 2.7 SPBC428.20c |alp6|SPBC902.01c|gamma tubulin complex Spc98/GCP3 s... 26 3.6 SPAC27D7.10c |||But2 family protein|Schizosaccharomyces pombe|ch... 26 4.8 SPAC27D7.09c |||But2 family protein|Schizosaccharomyces pombe|ch... 26 4.8 SPAC1834.02 |aro1||pentafunctional aromatic polypeptide Aro1 |Sc... 25 6.3 SPAPB24D3.02c |||amino acid permease, unknown 3|Schizosaccharomy... 25 6.3 SPAC24C9.14 |otu1|mug141|ubiquitin-specific protease |Schizosacc... 25 8.4 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 156 bits (379), Expect = 2e-39 Identities = 67/87 (77%), Positives = 79/87 (90%) Frame = +2 Query: 257 INVYYNEASGGKYVPRAVMVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHY 436 +NVY+NEA+GGKYVPRAV+VDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHY Sbjct: 47 LNVYFNEAAGGKYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHY 106 Query: 437 TEGAELVDSVLDVVRKEAEGCDCLRVF 517 TEGAEL D+VLDVVR+EAE CD L+ F Sbjct: 107 TEGAELADAVLDVVRREAEACDALQGF 133 Score = 75.4 bits (177), Expect = 6e-15 Identities = 31/45 (68%), Positives = 36/45 (80%) Frame = +3 Query: 120 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDATGAYSGDSDLQLE 254 MREIVHIQAGQCGNQ+GA FW I+DEHG+D+ G Y G S+ Q E Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHE 45 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 98.7 bits (235), Expect = 6e-22 Identities = 40/86 (46%), Positives = 59/86 (68%) Frame = +2 Query: 260 NVYYNEASGGKYVPRAVMVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYT 439 + +++E GKYVPR++ VDLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT Sbjct: 50 STFFSETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYT 109 Query: 440 EGAELVDSVLDVVRKEAEGCDCLRVF 517 G ELVD V D +R+ A+ C L+ F Sbjct: 110 VGKELVDEVTDKIRRIADNCSGLQGF 135 Score = 40.7 bits (91), Expect = 2e-04 Identities = 18/34 (52%), Positives = 20/34 (58%) Frame = +3 Query: 120 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDATG 221 MREI+ I GQ G QIG WE+ EHGI G Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNG 34 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 97.9 bits (233), Expect = 1e-21 Identities = 39/84 (46%), Positives = 60/84 (71%) Frame = +2 Query: 266 YYNEASGGKYVPRAVMVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEG 445 +++E GK+VPR++ VDLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G Sbjct: 56 FFSETGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVG 115 Query: 446 AELVDSVLDVVRKEAEGCDCLRVF 517 E++DSVL+ +R+ A+ C L+ F Sbjct: 116 KEMIDSVLERIRRMADNCSGLQGF 139 Score = 38.7 bits (86), Expect = 6e-04 Identities = 17/42 (40%), Positives = 25/42 (59%) Frame = +3 Query: 120 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDATGAYSGDSDL 245 MRE++ + GQ G QIG WE+ EHGI G + +S++ Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEV 42 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 77.8 bits (183), Expect = 1e-15 Identities = 30/89 (33%), Positives = 60/89 (67%), Gaps = 2/89 (2%) Frame = +2 Query: 260 NVYYNEASGGKYVPRAVMVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGH 433 +V++ ++ +Y+PRA+++DLEP ++++ S +G ++ P+N + ++ GAGNNWA G Sbjct: 49 DVFFYQSDDTRYIPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG- 107 Query: 434 YTEGAELVDSVLDVVRKEAEGCDCLRVFS 520 Y+ + + ++D++ +EA+G D L FS Sbjct: 108 YSHAERIFEDIMDMIDREADGSDSLEGFS 136 Score = 50.4 bits (115), Expect = 2e-07 Identities = 20/33 (60%), Positives = 26/33 (78%) Frame = +3 Query: 123 REIVHIQAGQCGNQIGAKFWEVISDEHGIDATG 221 REI+ +QAGQCGNQIG++FW+ + EHGI G Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDG 35 >SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1275 Score = 29.1 bits (62), Expect = 0.52 Identities = 16/62 (25%), Positives = 28/62 (45%) Frame = -1 Query: 526 ELAENPQTIAAFCFFSNYIQYRINQFSPLCVMXFRPVIPRPRLSEDEVVRSEDLPKRPGT 347 +L PQ AA+ +S++ + FSP + P + P ++ V LP+ P + Sbjct: 103 QLPNEPQNSAAYTTYSSFPNALFDDFSPNNPLDTDPFLTSPGNKQNTVDSFRPLPETPVS 162 Query: 346 DG 341 G Sbjct: 163 PG 164 >SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual Length = 234 Score = 28.3 bits (60), Expect = 0.90 Identities = 16/43 (37%), Positives = 23/43 (53%) Frame = +2 Query: 422 AKGHYTEGAELVDSVLDVVRKEAEGCDCLRVFS*LILWEEVLD 550 A+GH G ELV + D +RK++E L V I+ E L+ Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALEVCPDRIVCNEFLE 225 >SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 255 Score = 28.3 bits (60), Expect = 0.90 Identities = 14/32 (43%), Positives = 17/32 (53%) Frame = -2 Query: 411 PAPDCPKTKLSGRKICPNGPERTESMVPGSRS 316 P+ PK L R I PNGPE + + GS S Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51 >SPBC106.09 |cut4|apc1|anaphase-promoting complex subunit Apc1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1458 Score = 27.5 bits (58), Expect = 1.6 Identities = 17/58 (29%), Positives = 27/58 (46%) Frame = -3 Query: 284 LKLRYSRH*CFKLQVGVAAVSTRGVNAVLVRDYFPELCSDLIPALTRLDVDDFPHDCT 111 ++L SR C + G+ + V + +Y P S+ + + RLDVD F H T Sbjct: 599 VRLGISRKRCERYPFGILCIIFT-VLEIAAEEYSPNWESEELRLVNRLDVDSFLHPKT 655 >SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizosaccharomyces pombe|chr 1|||Manual Length = 506 Score = 26.6 bits (56), Expect = 2.7 Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%) Frame = +2 Query: 353 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 454 GP+G +F P F+F +G NW+ Y E A L Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190 >SPBC428.20c |alp6|SPBC902.01c|gamma tubulin complex Spc98/GCP3 subunit Alp6|Schizosaccharomyces pombe|chr 2|||Manual Length = 821 Score = 26.2 bits (55), Expect = 3.6 Identities = 10/35 (28%), Positives = 18/35 (51%) Frame = -3 Query: 188 YFPELCSDLIPALTRLDVDDFPHDCTKNLEPPESN 84 +F E+ + P +LD+DD + + P E+N Sbjct: 36 FFQEIIHSISPDTFQLDIDDILYKIYSKIPPEENN 70 >SPAC27D7.10c |||But2 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 383 Score = 25.8 bits (54), Expect = 4.8 Identities = 17/53 (32%), Positives = 26/53 (49%) Frame = +1 Query: 85 FDSGGSKFLVQS*GKSSTSRRVNAGIRSEQSSGK*SRTSTALTPRVLTAATPT 243 F S S S +STS RV++ ++ SSG + T+ + V +AT T Sbjct: 165 FVSPSSSSSSSSSAATSTSTRVSSSAKASTSSGAIAYTTKCVVVPVTASATAT 217 >SPAC27D7.09c |||But2 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 383 Score = 25.8 bits (54), Expect = 4.8 Identities = 17/53 (32%), Positives = 26/53 (49%) Frame = +1 Query: 85 FDSGGSKFLVQS*GKSSTSRRVNAGIRSEQSSGK*SRTSTALTPRVLTAATPT 243 F S S S +STS RV++ ++ SSG + T+ + V +AT T Sbjct: 165 FVSPSSSSSSSSSAATSTSTRVSSSAKASTSSGAIAYTTKCVVVPVTASATAT 217 >SPAC1834.02 |aro1||pentafunctional aromatic polypeptide Aro1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1573 Score = 25.4 bits (53), Expect = 6.3 Identities = 17/56 (30%), Positives = 25/56 (44%) Frame = +3 Query: 99 FQIFSAIMREIVHIQAGQCGNQIGAKFWEVISDEHGIDATGAYSGDSDLQLEASMS 266 F + S I I C + +W+V+ G+ TGA S SD L+ S+S Sbjct: 807 FSVLSLICPSRTLIIDKACVEKTWPYWWDVLHQSFGVKLTGATSVASD-PLKGSIS 861 >SPAPB24D3.02c |||amino acid permease, unknown 3|Schizosaccharomyces pombe|chr 1|||Manual Length = 543 Score = 25.4 bits (53), Expect = 6.3 Identities = 7/21 (33%), Positives = 14/21 (66%) Frame = -1 Query: 145 AWMWTISLMIALKIWNPPNRI 83 AW+W ++LM+ + PN++ Sbjct: 458 AWLWFMALMLLFPSYQNPNKV 478 >SPAC24C9.14 |otu1|mug141|ubiquitin-specific protease |Schizosaccharomyces pombe|chr 1|||Manual Length = 329 Score = 25.0 bits (52), Expect = 8.4 Identities = 9/21 (42%), Positives = 13/21 (61%) Frame = -3 Query: 461 NQPVQPPLCNXLSPSYSPPQT 399 N+P +PP+ N + PPQT Sbjct: 86 NEPAKPPIPNAATKPTFPPQT 106 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,525,532 Number of Sequences: 5004 Number of extensions: 52434 Number of successful extensions: 172 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 160 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 171 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 260219058 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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