Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= rbmte15m07
         (894 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC24B10.17 |emp24||COPII-coated vesicle component Emp24 |Schiz...   106   5e-24
SPAC17A5.08 |||COPII-coated vesicle component Erp2/3/4 |Schizosa...    64   3e-11
SPBC16E9.09c |||COPII vesicle coat component Erp5/Erp6|Schizosac...    59   1e-09
SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25 |Schiz...    44   2e-05
SPBC1604.05 |pgi1||glucose-6-phosphate isomerase |Schizosaccharo...    29   0.67 
SPBP19A11.03c |mts4|rpn1|19S proteasome regulatory subunit Mts4|...    28   2.1  
SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p...    27   3.6  
SPBP23A10.02 |||conserved fungal protein|Schizosaccharomyces pom...    27   4.8  

>SPCC24B10.17 |emp24||COPII-coated vesicle component Emp24
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 199

 Score =  106 bits (254), Expect = 5e-24
 Identities = 53/138 (38%), Positives = 79/138 (57%)
 Frame = -2

Query: 767 PDGNVIHKAERESSGIYTFSAPTAGKYTYCFSNKMSSMTPKVVMFNLEIGEPQTKSGNEN 588
           P G ++H+    S   Y+F+    GKY YCF N       K V+FN+          +E+
Sbjct: 65  PAGQIMHQEVPNSMAQYSFTVKNPGKYMYCFYNDALDGESKEVLFNVH---GVIYISDED 121

Query: 587 EADHNKLEDMIKELATTLRTIKHDQEYMQVRDRIHRSINESTNSRVVMWSIFEASVLLVM 408
              +N L   +++L  T+  +KH+QEY   R+RIHR+  ESTN RV  WSI +  +L+ +
Sbjct: 122 LDANNPLLGKVRQLHDTISKVKHEQEYFVARERIHRNTAESTNDRVKWWSILQTVILVSV 181

Query: 407 TIGQVYYLKRFFEVQRVV 354
            + Q++YLKR FEV+RVV
Sbjct: 182 CVFQIFYLKRLFEVKRVV 199



 Score = 29.5 bits (63), Expect = 0.67
 Identities = 11/37 (29%), Positives = 21/37 (56%)
 Frame = -1

Query: 876 HAXECFYENVEADTKLGLSFEIAEGGFLDIDVIILGP 766
           H  ECFYEN+  + ++ ++++   GG   + + I  P
Sbjct: 29  HQRECFYENLRNNDQMSVTYQTNVGGDQLVSMSIYNP 65


>SPAC17A5.08 |||COPII-coated vesicle component Erp2/3/4
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 210

 Score = 63.7 bits (148), Expect = 3e-11
 Identities = 33/138 (23%), Positives = 67/138 (48%), Gaps = 4/138 (2%)
 Frame = -2

Query: 770 APDGNVIHKAERESSGIYTFSAPTAGKYTYCFSNKMSSMTPKVVMFNL----EIGEPQTK 603
           AP    +   ++       F+    G+Y +CF N MS+ T K+V   +    E+  P   
Sbjct: 66  APSQKTVALGKKRRQADVFFTLEEKGEYEFCFDNHMSTFTDKIVTMEITMENELSLPALT 125

Query: 602 SGNENEADHNKLEDMIKELATTLRTIKHDQEYMQVRDRIHRSINESTNSRVVMWSIFEAS 423
                +   + ++  + E++T L  I   Q Y + R+  + S  +ST +R+  +S+ E+ 
Sbjct: 126 RDEAKDYKKDSMQSTVLEISTALSEIDRVQNYFKTREHRNYSTVKSTQARIFWFSLAESI 185

Query: 422 VLLVMTIGQVYYLKRFFE 369
           +++ ++  QV+ +K FF+
Sbjct: 186 MVVALSALQVFIVKTFFK 203


>SPBC16E9.09c |||COPII vesicle coat component
           Erp5/Erp6|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 215

 Score = 58.8 bits (136), Expect = 1e-09
 Identities = 38/140 (27%), Positives = 65/140 (46%), Gaps = 3/140 (2%)
 Frame = -2

Query: 764 DGNVIHKAERESSGIYTFSAPTAGKYTYCFSNKMSSMTPKVVMFNLEIGEPQTKSGN--- 594
           + ++I+K +  S+    F+    G +  C+ +             L +        N   
Sbjct: 76  NNHLIYKNDLRSNEAVRFTTTNEGTHRICYLSTSDGAWFSSTKTRLHVDLASDDKYNLIA 135

Query: 593 ENEADHNKLEDMIKELATTLRTIKHDQEYMQVRDRIHRSINESTNSRVVMWSIFEASVLL 414
            +E +   L D +  L T L +I  +Q   + R+   R  +ES NSRVV W+I +  VLL
Sbjct: 136 SSEDEAKDLTDRVAALNTRLESILGEQMLQRSREMTFRDTSESANSRVVRWTIVQIVVLL 195

Query: 413 VMTIGQVYYLKRFFEVQRVV 354
           V  I Q+ +L+RFF  +++V
Sbjct: 196 VTCIWQLSHLQRFFVKEKLV 215


>SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 216

 Score = 44.4 bits (100), Expect = 2e-05
 Identities = 27/78 (34%), Positives = 41/78 (52%)
 Frame = -2

Query: 587 EADHNKLEDMIKELATTLRTIKHDQEYMQVRDRIHRSINESTNSRVVMWSIFEASVLLVM 408
           EAD  +  D I+E       IK    Y+Q R+   R+ NESTN RV  ++      L V+
Sbjct: 145 EADIRRARDFIEE-------IKGKIYYLQAREARFRNTNESTNERVKNFAYLTFISLFVL 197

Query: 407 TIGQVYYLKRFFEVQRVV 354
            I Q+ YL+ FF+ + ++
Sbjct: 198 VIWQILYLRSFFQRKHLI 215


>SPBC1604.05 |pgi1||glucose-6-phosphate isomerase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 550

 Score = 29.5 bits (63), Expect = 0.67
 Identities = 12/38 (31%), Positives = 21/38 (55%)
 Frame = -3

Query: 769 PLMATSFIKQRGSHLVFIHFLLQLQGSILIASVIKCHP 656
           P+M T  +K  G   + +HF+  + G+ L  ++ KC P
Sbjct: 169 PVMVTEALKPYGQENLELHFVSNIDGTHLAEALKKCDP 206


>SPBP19A11.03c |mts4|rpn1|19S proteasome regulatory subunit
           Mts4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 891

 Score = 27.9 bits (59), Expect = 2.1
 Identities = 17/48 (35%), Positives = 25/48 (52%), Gaps = 3/48 (6%)
 Frame = +1

Query: 400 PIVMTSSTEASNID---HITTREFVLSFIERWIRSRTCIYS*SCLIVL 534
           P  +T + EA  ID    +   E V+ F+E    SR C+Y  SC+ +L
Sbjct: 195 PFFLTHNAEADAIDLLQELGAIEKVVPFVELDNASRVCLYITSCVNLL 242


>SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 676

 Score = 27.1 bits (57), Expect = 3.6
 Identities = 13/29 (44%), Positives = 21/29 (72%), Gaps = 2/29 (6%)
 Frame = -2

Query: 452 VVMWSIFEASVLLVM-TIGQVYYL-KRFF 372
           +V+W +F+  VLLV  T+G  ++L K+FF
Sbjct: 560 LVLWMLFQVLVLLVQDTLGPRFFLPKKFF 588


>SPBP23A10.02 |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 132

 Score = 26.6 bits (56), Expect = 4.8
 Identities = 11/42 (26%), Positives = 23/42 (54%)
 Frame = -2

Query: 602 SGNENEADHNKLEDMIKELATTLRTIKHDQEYMQVRDRIHRS 477
           S N+N +++ ++ D   + ATT +T    +E ++ R   + S
Sbjct: 91  SNNQNPSNNKEMSDKESDSATTTQTFSVPEELLRARTTANNS 132


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,489,473
Number of Sequences: 5004
Number of extensions: 70498
Number of successful extensions: 174
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 164
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 172
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 450492750
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)