BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= rbmte15b17
(737 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1198.03c |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 30 0.40
SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosac... 27 2.1
SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccha... 27 2.1
SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 27 3.7
SPBC36.08c |cog2||Golgi transport complex subunit Cog2 |Schizosa... 27 3.7
SPAC26F1.09 |gyp51||GTPase activating protein Gyp51 |Schizosacch... 27 3.7
SPAC806.08c |mod21||gamma tubulin complex subunit Mod21|Schizosa... 25 8.5
>SPBC1198.03c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 249
Score = 29.9 bits (64), Expect = 0.40
Identities = 20/63 (31%), Positives = 33/63 (52%), Gaps = 1/63 (1%)
Frame = +3
Query: 15 VSFKSIEPFLHTICNSLCGSFSNRNLFGCESSMNLPSFNILSSTVIFSAIRFV-AGKLFI 191
+S K + F+H + N C SFS+ L G + +NL SF + + + + ++V K I
Sbjct: 114 ISGKDWKSFIHDV-NMAC-SFSDVALMGLDQILNLVSFGLSAYVISYYVEKYVDKAKFPI 171
Query: 192 ISG 200
I G
Sbjct: 172 IKG 174
>SPAC18G6.05c |||translation elongation regulator Gcn1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2670
Score = 27.5 bits (58), Expect = 2.1
Identities = 16/74 (21%), Positives = 42/74 (56%), Gaps = 1/74 (1%)
Frame = -2
Query: 304 IQASLKSGEVYMHASQLMEKSSIFIHEKGKVELLIPDIINNLPATNLIAEKITV-DDKIL 128
+++ + S E+ S L+ +++ + +EL + DI+ ++ +T + +++T+ ++ IL
Sbjct: 2445 LESKVLSLEIQSEFSVLILNAAVKFGSQKIIELKLSDIVCSIISTASLQKEVTIAENGIL 2504
Query: 127 KLGRFMDDSHPKRF 86
LG+ + P+ F
Sbjct: 2505 ALGKALLADIPQSF 2518
>SPBC83.01 |ucp8||UBA/EH/EF hand domain protein
Ucp8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 884
Score = 27.5 bits (58), Expect = 2.1
Identities = 20/90 (22%), Positives = 37/90 (41%)
Frame = -2
Query: 463 STHAGPIAVKSCYSDNSHFTTLMGNIRLDHLHRSVLIDIIQRGNLHITGFSGNIQASLKS 284
S H G + + Y H + L HL RS+ D+I+ + NI+ +
Sbjct: 181 SAHVGYLEMYQ-YVIARHLVAICKQYNLIHLPRSLPADVIESAKSETPAMNTNIEQGVTQ 239
Query: 283 GEVYMHASQLMEKSSIFIHEKGKVELLIPD 194
+ ++ QL + SI + K++ + D
Sbjct: 240 PSL-VNTEQLSHQQSISSTKSSKLDEISAD 268
>SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 583
Score = 26.6 bits (56), Expect = 3.7
Identities = 12/52 (23%), Positives = 24/52 (46%)
Frame = -2
Query: 544 QASNIELKTGLNSGIKCHNVMANSFIVSTHAGPIAVKSCYSDNSHFTTLMGN 389
Q++N+E + ++ ++ NV + F+ HAG + N L+ N
Sbjct: 267 QSNNLEFPSSISGNVESGNVAFDEFLSLLHAGNLVQAMNAETNPRTAELIAN 318
>SPBC36.08c |cog2||Golgi transport complex subunit Cog2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 191
Score = 26.6 bits (56), Expect = 3.7
Identities = 18/73 (24%), Positives = 31/73 (42%)
Frame = -2
Query: 313 SGNIQASLKSGEVYMHASQLMEKSSIFIHEKGKVELLIPDIINNLPATNLIAEKITVDDK 134
SGN + S ++ QL I +++ + ++ LIA + + D+
Sbjct: 85 SGNTKVSTLISSIHRSEEQLKNSKQSLIGHSTEIQNNLKHK-QDVENEKLIASNLLLLDQ 143
Query: 133 ILKLGRFMDDSHP 95
ILK + D SHP
Sbjct: 144 ILKFLKSNDSSHP 156
>SPAC26F1.09 |gyp51||GTPase activating protein Gyp51
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1031
Score = 26.6 bits (56), Expect = 3.7
Identities = 24/100 (24%), Positives = 50/100 (50%), Gaps = 3/100 (3%)
Frame = -2
Query: 418 NSHFTTL---MGNIRLDHLHRSVLIDIIQRGNLHITGFSGNIQASLKSGEVYMHASQLME 248
N HF L M N++++H + S L+ + +++ + QASLKS +++ +
Sbjct: 905 NEHFIMLSDSMANLQVEHENMSALL---LKEKMYLKNQTVE-QASLKSEIASLNSQLAKQ 960
Query: 247 KSSIFIHEKGKVELLIPDIINNLPATNLIAEKITVDDKIL 128
KS I +G +E +I + + + + + ++I +K L
Sbjct: 961 KSEIEQAFQGDMEAIIAENLEIMVESQSLEDEIFRKEKQL 1000
>SPAC806.08c |mod21||gamma tubulin complex subunit
Mod21|Schizosaccharomyces pombe|chr 1|||Manual
Length = 618
Score = 25.4 bits (53), Expect = 8.5
Identities = 16/43 (37%), Positives = 25/43 (58%), Gaps = 3/43 (6%)
Frame = -2
Query: 226 EKGKVELLIPDIINNLPAT--NLIAE-KITVDDKILKLGRFMD 107
EK + L + I+ + T NL+ E +I + KILKLG+ +D
Sbjct: 390 EKDNIALQLGSIVFDQSPTLNNLLVEMEILIRSKILKLGKSLD 432
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,910,059
Number of Sequences: 5004
Number of extensions: 58472
Number of successful extensions: 135
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 132
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 135
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 349251756
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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