Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= rbmte14g09
(704 letters)
Database: fruitfly
53,049 sequences; 24,988,368 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AY089443-1|AAL90181.1| 366|Drosophila melanogaster AT26187p pro... 37 0.031
AE014298-1321|AAF46482.2| 366|Drosophila melanogaster CG17440-P... 37 0.031
Z50152-2|CAA90514.1| 3358|Drosophila melanogaster trithorax prot... 36 0.071
Z50152-1|CAA90513.1| 3726|Drosophila melanogaster trithorax prot... 36 0.071
Z31725-2|CAA83515.1| 3358|Drosophila melanogaster predicted trit... 36 0.071
Z31725-1|CAA83516.1| 3726|Drosophila melanogaster predicted trit... 36 0.071
M31617-1|AAA29025.1| 3759|Drosophila melanogaster protein ( D.me... 36 0.071
AE014297-1828|AAX52951.1| 3358|Drosophila melanogaster CG8651-PE... 36 0.071
AE014297-1827|AAN13601.1| 3358|Drosophila melanogaster CG8651-PC... 36 0.071
AE014297-1826|AAN13600.1| 3358|Drosophila melanogaster CG8651-PB... 36 0.071
AE014297-1825|AAN13599.1| 3726|Drosophila melanogaster CG8651-PD... 36 0.071
AE014297-1824|AAF55041.2| 3726|Drosophila melanogaster CG8651-PA... 36 0.071
AF417921-1|AAL16644.1| 2669|Drosophila melanogaster nucleosome r... 35 0.12
AE014296-61|AAN11431.1| 2669|Drosophila melanogaster CG32346-PA,... 35 0.12
AE014296-60|AAF47361.2| 2649|Drosophila melanogaster CG32346-PB,... 35 0.12
AE014298-1322|AAF46483.1| 663|Drosophila melanogaster CG17446-P... 34 0.22
Y14999-1|CAB40628.1| 1408|Drosophila melanogaster Bip2 protein p... 32 0.87
AY069276-1|AAL39421.1| 363|Drosophila melanogaster GM10839p pro... 32 0.87
AJ292191-1|CAC34474.1| 1408|Drosophila melanogaster TAFII155 pro... 32 0.87
AJ251945-1|CAB64265.1| 1406|Drosophila melanogaster BIP2 protein... 32 0.87
AE014135-85|AAF59342.1| 1406|Drosophila melanogaster CG2009-PA p... 32 0.87
AE014297-1323|AAF54658.2| 788|Drosophila melanogaster CG5342-PA... 30 2.7
AE014296-2342|AAF49773.3| 3146|Drosophila melanogaster CG9007-PA... 30 2.7
BT003500-1|AAO39504.1| 1169|Drosophila melanogaster RE45036p pro... 30 3.5
AE013599-158|AAF57274.2| 1169|Drosophila melanogaster CG11212-PA... 30 3.5
U09306-1|AAB60255.1| 1106|Drosophila melanogaster shuttle craft ... 29 8.1
AF145679-1|AAD38654.1| 1106|Drosophila melanogaster BcDNA.LD2272... 29 8.1
AE014134-2570|AAN10891.1| 1099|Drosophila melanogaster CG3647-PA... 29 8.1
AE014134-2569|AAF53441.2| 1106|Drosophila melanogaster CG3647-PB... 29 8.1
>AY089443-1|AAL90181.1| 366|Drosophila melanogaster AT26187p
protein.
Length = 366
Score = 36.7 bits (81), Expect = 0.031
Identities = 14/38 (36%), Positives = 20/38 (52%)
Frame = -3
Query: 237 IGCDNCLAWYHLKCTIFSSEDYENVYNKEFVCPTCNNK 124
IGCD C WYH C + +D E++ K + C C +
Sbjct: 53 IGCDGCEEWYHGDCIEITEKDAEHI--KNYYCRRCKKE 88
>AE014298-1321|AAF46482.2| 366|Drosophila melanogaster CG17440-PA
protein.
Length = 366
Score = 36.7 bits (81), Expect = 0.031
Identities = 14/38 (36%), Positives = 20/38 (52%)
Frame = -3
Query: 237 IGCDNCLAWYHLKCTIFSSEDYENVYNKEFVCPTCNNK 124
IGCD C WYH C + +D E++ K + C C +
Sbjct: 53 IGCDGCEEWYHGDCIEITEKDAEHI--KNYYCRRCKKE 88
>Z50152-2|CAA90514.1| 3358|Drosophila melanogaster trithorax protein
trxI protein.
Length = 3358
Score = 35.5 bits (78), Expect = 0.071
Identities = 22/72 (30%), Positives = 35/72 (48%), Gaps = 9/72 (12%)
Frame = -3
Query: 294 CDSCEEELTNDTDINVLKNIGCDNCLAWYHLKCTIFSSEDYENV----YNKEFVCPTC-- 133
C C ++ ND D+ +++ C +C W H KC S E Y + + EF+C C
Sbjct: 1059 CQRCYDD--NDFDLKMME---CGDCGQWVHSKCEGLSDEQYNLLSTLPESIEFICKKCAR 1113
Query: 132 ---NNK*KADYW 106
++K KA+ W
Sbjct: 1114 RNESSKIKAEEW 1125
>Z50152-1|CAA90513.1| 3726|Drosophila melanogaster trithorax protein
trxII protein.
Length = 3726
Score = 35.5 bits (78), Expect = 0.071
Identities = 22/72 (30%), Positives = 35/72 (48%), Gaps = 9/72 (12%)
Frame = -3
Query: 294 CDSCEEELTNDTDINVLKNIGCDNCLAWYHLKCTIFSSEDYENV----YNKEFVCPTC-- 133
C C ++ ND D+ +++ C +C W H KC S E Y + + EF+C C
Sbjct: 1427 CQRCYDD--NDFDLKMME---CGDCGQWVHSKCEGLSDEQYNLLSTLPESIEFICKKCAR 1481
Query: 132 ---NNK*KADYW 106
++K KA+ W
Sbjct: 1482 RNESSKIKAEEW 1493
>Z31725-2|CAA83515.1| 3358|Drosophila melanogaster predicted trithorax
protein protein.
Length = 3358
Score = 35.5 bits (78), Expect = 0.071
Identities = 22/72 (30%), Positives = 35/72 (48%), Gaps = 9/72 (12%)
Frame = -3
Query: 294 CDSCEEELTNDTDINVLKNIGCDNCLAWYHLKCTIFSSEDYENV----YNKEFVCPTC-- 133
C C ++ ND D+ +++ C +C W H KC S E Y + + EF+C C
Sbjct: 1059 CQRCYDD--NDFDLKMME---CGDCGQWVHSKCEGLSDEQYNLLSTLPESIEFICKKCAR 1113
Query: 132 ---NNK*KADYW 106
++K KA+ W
Sbjct: 1114 RNESSKIKAEEW 1125
>Z31725-1|CAA83516.1| 3726|Drosophila melanogaster predicted trithorax
protein protein.
Length = 3726
Score = 35.5 bits (78), Expect = 0.071
Identities = 22/72 (30%), Positives = 35/72 (48%), Gaps = 9/72 (12%)
Frame = -3
Query: 294 CDSCEEELTNDTDINVLKNIGCDNCLAWYHLKCTIFSSEDYENV----YNKEFVCPTC-- 133
C C ++ ND D+ +++ C +C W H KC S E Y + + EF+C C
Sbjct: 1427 CQRCYDD--NDFDLKMME---CGDCGQWVHSKCEGLSDEQYNLLSTLPESIEFICKKCAR 1481
Query: 132 ---NNK*KADYW 106
++K KA+ W
Sbjct: 1482 RNESSKIKAEEW 1493
>M31617-1|AAA29025.1| 3759|Drosophila melanogaster protein (
D.melanogaster zinc-binding protein (trithorax) mRNA,
complete cds. ).
Length = 3759
Score = 35.5 bits (78), Expect = 0.071
Identities = 22/72 (30%), Positives = 35/72 (48%), Gaps = 9/72 (12%)
Frame = -3
Query: 294 CDSCEEELTNDTDINVLKNIGCDNCLAWYHLKCTIFSSEDYENV----YNKEFVCPTC-- 133
C C ++ ND D+ +++ C +C W H KC S E Y + + EF+C C
Sbjct: 1427 CQRCYDD--NDFDLKMME---CGDCGQWVHSKCEGLSDEQYNLLSTLPESIEFICKKCAR 1481
Query: 132 ---NNK*KADYW 106
++K KA+ W
Sbjct: 1482 RNESSKIKAEEW 1493
>AE014297-1828|AAX52951.1| 3358|Drosophila melanogaster CG8651-PE,
isoform E protein.
Length = 3358
Score = 35.5 bits (78), Expect = 0.071
Identities = 22/72 (30%), Positives = 35/72 (48%), Gaps = 9/72 (12%)
Frame = -3
Query: 294 CDSCEEELTNDTDINVLKNIGCDNCLAWYHLKCTIFSSEDYENV----YNKEFVCPTC-- 133
C C ++ ND D+ +++ C +C W H KC S E Y + + EF+C C
Sbjct: 1059 CQRCYDD--NDFDLKMME---CGDCGQWVHSKCEGLSDEQYNLLSTLPESIEFICKKCAR 1113
Query: 132 ---NNK*KADYW 106
++K KA+ W
Sbjct: 1114 RNESSKIKAEEW 1125
>AE014297-1827|AAN13601.1| 3358|Drosophila melanogaster CG8651-PC,
isoform C protein.
Length = 3358
Score = 35.5 bits (78), Expect = 0.071
Identities = 22/72 (30%), Positives = 35/72 (48%), Gaps = 9/72 (12%)
Frame = -3
Query: 294 CDSCEEELTNDTDINVLKNIGCDNCLAWYHLKCTIFSSEDYENV----YNKEFVCPTC-- 133
C C ++ ND D+ +++ C +C W H KC S E Y + + EF+C C
Sbjct: 1059 CQRCYDD--NDFDLKMME---CGDCGQWVHSKCEGLSDEQYNLLSTLPESIEFICKKCAR 1113
Query: 132 ---NNK*KADYW 106
++K KA+ W
Sbjct: 1114 RNESSKIKAEEW 1125
>AE014297-1826|AAN13600.1| 3358|Drosophila melanogaster CG8651-PB,
isoform B protein.
Length = 3358
Score = 35.5 bits (78), Expect = 0.071
Identities = 22/72 (30%), Positives = 35/72 (48%), Gaps = 9/72 (12%)
Frame = -3
Query: 294 CDSCEEELTNDTDINVLKNIGCDNCLAWYHLKCTIFSSEDYENV----YNKEFVCPTC-- 133
C C ++ ND D+ +++ C +C W H KC S E Y + + EF+C C
Sbjct: 1059 CQRCYDD--NDFDLKMME---CGDCGQWVHSKCEGLSDEQYNLLSTLPESIEFICKKCAR 1113
Query: 132 ---NNK*KADYW 106
++K KA+ W
Sbjct: 1114 RNESSKIKAEEW 1125
>AE014297-1825|AAN13599.1| 3726|Drosophila melanogaster CG8651-PD,
isoform D protein.
Length = 3726
Score = 35.5 bits (78), Expect = 0.071
Identities = 22/72 (30%), Positives = 35/72 (48%), Gaps = 9/72 (12%)
Frame = -3
Query: 294 CDSCEEELTNDTDINVLKNIGCDNCLAWYHLKCTIFSSEDYENV----YNKEFVCPTC-- 133
C C ++ ND D+ +++ C +C W H KC S E Y + + EF+C C
Sbjct: 1427 CQRCYDD--NDFDLKMME---CGDCGQWVHSKCEGLSDEQYNLLSTLPESIEFICKKCAR 1481
Query: 132 ---NNK*KADYW 106
++K KA+ W
Sbjct: 1482 RNESSKIKAEEW 1493
>AE014297-1824|AAF55041.2| 3726|Drosophila melanogaster CG8651-PA,
isoform A protein.
Length = 3726
Score = 35.5 bits (78), Expect = 0.071
Identities = 22/72 (30%), Positives = 35/72 (48%), Gaps = 9/72 (12%)
Frame = -3
Query: 294 CDSCEEELTNDTDINVLKNIGCDNCLAWYHLKCTIFSSEDYENV----YNKEFVCPTC-- 133
C C ++ ND D+ +++ C +C W H KC S E Y + + EF+C C
Sbjct: 1427 CQRCYDD--NDFDLKMME---CGDCGQWVHSKCEGLSDEQYNLLSTLPESIEFICKKCAR 1481
Query: 132 ---NNK*KADYW 106
++K KA+ W
Sbjct: 1482 RNESSKIKAEEW 1493
>AF417921-1|AAL16644.1| 2669|Drosophila melanogaster nucleosome
remodeling factor largesubunit NURF301 protein.
Length = 2669
Score = 34.7 bits (76), Expect = 0.12
Identities = 14/38 (36%), Positives = 19/38 (50%)
Frame = -3
Query: 237 IGCDNCLAWYHLKCTIFSSEDYENVYNKEFVCPTCNNK 124
I CD C W+H +C + E + E+VCP C K
Sbjct: 2511 ICCDKCQDWFHGRCVGILQSEAE--FIDEYVCPECQRK 2546
Score = 31.9 bits (69), Expect = 0.87
Identities = 12/35 (34%), Positives = 18/35 (51%)
Frame = -3
Query: 237 IGCDNCLAWYHLKCTIFSSEDYENVYNKEFVCPTC 133
+GCD C W+H C + E + + EF+C C
Sbjct: 2455 VGCDLCSNWFHGDCVSITEEASKKL--SEFICIDC 2487
>AE014296-61|AAN11431.1| 2669|Drosophila melanogaster CG32346-PA,
isoform A protein.
Length = 2669
Score = 34.7 bits (76), Expect = 0.12
Identities = 14/38 (36%), Positives = 19/38 (50%)
Frame = -3
Query: 237 IGCDNCLAWYHLKCTIFSSEDYENVYNKEFVCPTCNNK 124
I CD C W+H +C + E + E+VCP C K
Sbjct: 2511 ICCDKCQDWFHGRCVGILQSEAE--FIDEYVCPECQRK 2546
Score = 31.9 bits (69), Expect = 0.87
Identities = 12/35 (34%), Positives = 18/35 (51%)
Frame = -3
Query: 237 IGCDNCLAWYHLKCTIFSSEDYENVYNKEFVCPTC 133
+GCD C W+H C + E + + EF+C C
Sbjct: 2455 VGCDLCSNWFHGDCVSITEEASKKL--SEFICIDC 2487
>AE014296-60|AAF47361.2| 2649|Drosophila melanogaster CG32346-PB,
isoform B protein.
Length = 2649
Score = 34.7 bits (76), Expect = 0.12
Identities = 14/38 (36%), Positives = 19/38 (50%)
Frame = -3
Query: 237 IGCDNCLAWYHLKCTIFSSEDYENVYNKEFVCPTCNNK 124
I CD C W+H +C + E + E+VCP C K
Sbjct: 2491 ICCDKCQDWFHGRCVGILQSEAE--FIDEYVCPECQRK 2526
Score = 31.9 bits (69), Expect = 0.87
Identities = 12/35 (34%), Positives = 18/35 (51%)
Frame = -3
Query: 237 IGCDNCLAWYHLKCTIFSSEDYENVYNKEFVCPTC 133
+GCD C W+H C + E + + EF+C C
Sbjct: 2435 VGCDLCSNWFHGDCVSITEEASKKL--SEFICIDC 2467
>AE014298-1322|AAF46483.1| 663|Drosophila melanogaster CG17446-PA
protein.
Length = 663
Score = 33.9 bits (74), Expect = 0.22
Identities = 12/38 (31%), Positives = 21/38 (55%)
Frame = -3
Query: 237 IGCDNCLAWYHLKCTIFSSEDYENVYNKEFVCPTCNNK 124
IGCD C WYH C + ++ +++ K++ C C +
Sbjct: 75 IGCDGCEEWYHGDCIGITEKEAKHI--KQYYCRRCKKE 110
>Y14999-1|CAB40628.1| 1408|Drosophila melanogaster Bip2 protein
protein.
Length = 1408
Score = 31.9 bits (69), Expect = 0.87
Identities = 16/40 (40%), Positives = 20/40 (50%), Gaps = 2/40 (5%)
Frame = -3
Query: 237 IGCDNCLAWYHLKCT--IFSSEDYENVYNKEFVCPTCNNK 124
IGCD C AWYH C F+ +D N ++ C C K
Sbjct: 1358 IGCDGCDAWYHWICVGITFAPKD-----NDDWFCRVCVTK 1392
>AY069276-1|AAL39421.1| 363|Drosophila melanogaster GM10839p
protein.
Length = 363
Score = 31.9 bits (69), Expect = 0.87
Identities = 16/40 (40%), Positives = 20/40 (50%), Gaps = 2/40 (5%)
Frame = -3
Query: 237 IGCDNCLAWYHLKCT--IFSSEDYENVYNKEFVCPTCNNK 124
IGCD C AWYH C F+ +D N ++ C C K
Sbjct: 313 IGCDGCDAWYHWICVGITFAPKD-----NDDWFCRVCVTK 347
>AJ292191-1|CAC34474.1| 1408|Drosophila melanogaster TAFII155 protein
protein.
Length = 1408
Score = 31.9 bits (69), Expect = 0.87
Identities = 16/40 (40%), Positives = 20/40 (50%), Gaps = 2/40 (5%)
Frame = -3
Query: 237 IGCDNCLAWYHLKCT--IFSSEDYENVYNKEFVCPTCNNK 124
IGCD C AWYH C F+ +D N ++ C C K
Sbjct: 1358 IGCDGCDAWYHWICVGITFAPKD-----NDDWFCRVCVTK 1392
>AJ251945-1|CAB64265.1| 1406|Drosophila melanogaster BIP2 protein
protein.
Length = 1406
Score = 31.9 bits (69), Expect = 0.87
Identities = 16/40 (40%), Positives = 20/40 (50%), Gaps = 2/40 (5%)
Frame = -3
Query: 237 IGCDNCLAWYHLKCT--IFSSEDYENVYNKEFVCPTCNNK 124
IGCD C AWYH C F+ +D N ++ C C K
Sbjct: 1356 IGCDGCDAWYHWICVGITFAPKD-----NDDWFCRVCVTK 1390
>AE014135-85|AAF59342.1| 1406|Drosophila melanogaster CG2009-PA
protein.
Length = 1406
Score = 31.9 bits (69), Expect = 0.87
Identities = 16/40 (40%), Positives = 20/40 (50%), Gaps = 2/40 (5%)
Frame = -3
Query: 237 IGCDNCLAWYHLKCT--IFSSEDYENVYNKEFVCPTCNNK 124
IGCD C AWYH C F+ +D N ++ C C K
Sbjct: 1356 IGCDGCDAWYHWICVGITFAPKD-----NDDWFCRVCVTK 1390
>AE014297-1323|AAF54658.2| 788|Drosophila melanogaster CG5342-PA
protein.
Length = 788
Score = 30.3 bits (65), Expect = 2.7
Identities = 13/33 (39%), Positives = 17/33 (51%)
Frame = -2
Query: 106 GKTWLTCGEMRLTTGKKCHILFNIDFLYNSNKL 8
G W+ C LT GKKC L I + +S +L
Sbjct: 369 GIAWIYCLRCHLTVGKKCRNLIEIQEVISSKRL 401
>AE014296-2342|AAF49773.3| 3146|Drosophila melanogaster CG9007-PA
protein.
Length = 3146
Score = 30.3 bits (65), Expect = 2.7
Identities = 17/51 (33%), Positives = 25/51 (49%)
Frame = -3
Query: 285 CEEELTNDTDINVLKNIGCDNCLAWYHLKCTIFSSEDYENVYNKEFVCPTC 133
C ELT+D + CD C AW H+ C D +N+ +E++C C
Sbjct: 857 CICELTHDDGYMIC----CDKCSAWQHVDC---MGIDRQNI-PEEYMCELC 899
>BT003500-1|AAO39504.1| 1169|Drosophila melanogaster RE45036p
protein.
Length = 1169
Score = 29.9 bits (64), Expect = 3.5
Identities = 18/43 (41%), Positives = 22/43 (51%), Gaps = 1/43 (2%)
Frame = +1
Query: 220 TIVTTNIF*DVYICVISQFLFTRI-ASYSVYCACRTFLLTYTF 345
TI + F I +IS F RI +YSV+ C TFL TF
Sbjct: 378 TITSVTDFISFLIGIISPFRSVRIFCTYSVFAVCFTFLWHITF 420
>AE013599-158|AAF57274.2| 1169|Drosophila melanogaster CG11212-PA
protein.
Length = 1169
Score = 29.9 bits (64), Expect = 3.5
Identities = 18/43 (41%), Positives = 22/43 (51%), Gaps = 1/43 (2%)
Frame = +1
Query: 220 TIVTTNIF*DVYICVISQFLFTRI-ASYSVYCACRTFLLTYTF 345
TI + F I +IS F RI +YSV+ C TFL TF
Sbjct: 378 TITSVTDFISFLIGIISPFRSVRIFCTYSVFAVCFTFLWHITF 420
>U09306-1|AAB60255.1| 1106|Drosophila melanogaster shuttle craft
protein protein.
Length = 1106
Score = 28.7 bits (61), Expect = 8.1
Identities = 15/36 (41%), Positives = 18/36 (50%), Gaps = 1/36 (2%)
Frame = -3
Query: 231 CDNCLAWYHLKCTI-FSSEDYENVYNKEFVCPTCNN 127
C NC HLKCTI ++S V + CP C N
Sbjct: 402 CRNCYHMLHLKCTITWASSSKSEV---GWRCPACQN 434
>AF145679-1|AAD38654.1| 1106|Drosophila melanogaster BcDNA.LD22726
protein.
Length = 1106
Score = 28.7 bits (61), Expect = 8.1
Identities = 15/36 (41%), Positives = 18/36 (50%), Gaps = 1/36 (2%)
Frame = -3
Query: 231 CDNCLAWYHLKCTI-FSSEDYENVYNKEFVCPTCNN 127
C NC HLKCTI ++S V + CP C N
Sbjct: 402 CRNCYHMLHLKCTITWASSSKSEV---GWRCPACQN 434
>AE014134-2570|AAN10891.1| 1099|Drosophila melanogaster CG3647-PA,
isoform A protein.
Length = 1099
Score = 28.7 bits (61), Expect = 8.1
Identities = 15/36 (41%), Positives = 18/36 (50%), Gaps = 1/36 (2%)
Frame = -3
Query: 231 CDNCLAWYHLKCTI-FSSEDYENVYNKEFVCPTCNN 127
C NC HLKCTI ++S V + CP C N
Sbjct: 395 CRNCYHMLHLKCTITWASSSKSEV---GWRCPACQN 427
>AE014134-2569|AAF53441.2| 1106|Drosophila melanogaster CG3647-PB,
isoform B protein.
Length = 1106
Score = 28.7 bits (61), Expect = 8.1
Identities = 15/36 (41%), Positives = 18/36 (50%), Gaps = 1/36 (2%)
Frame = -3
Query: 231 CDNCLAWYHLKCTI-FSSEDYENVYNKEFVCPTCNN 127
C NC HLKCTI ++S V + CP C N
Sbjct: 402 CRNCYHMLHLKCTITWASSSKSEV---GWRCPACQN 434
Database: fruitfly
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 24,988,368
Number of sequences in database: 53,049
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 22,961,082
Number of Sequences: 53049
Number of extensions: 401682
Number of successful extensions: 1138
Number of sequences better than 10.0: 29
Number of HSP's better than 10.0 without gapping: 1087
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1135
length of database: 24,988,368
effective HSP length: 83
effective length of database: 20,585,301
effective search space used: 3108380451
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -