Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= rbmte14a17
(825 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC6G10.08 |idp1||isocitrate dehydrogenase Idp1|Schizosaccharom... 294 1e-80
SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces ... 32 0.11
SPBC902.05c |idh2|glu2|isocitrate dehydrogenase |Schizosaccharom... 28 1.4
SPBC16A3.13 |meu7|aah4|alpha-amylase homolog Aah4|Schizosaccharo... 27 2.4
SPBC16H5.10c |prp43||ATP-dependent RNA helicase Prp43|Schizosacc... 27 3.2
SPBC27.01c |||secretory pathway protein Pga2 |Schizosaccharomyce... 27 4.3
SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3 |Schizosa... 27 4.3
SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase Cdc... 26 5.6
SPAC22A12.11 |dak1||dihydroxyacetone kinase Dak1|Schizosaccharom... 25 9.9
SPAC926.02 |||conserved fungal protein|Schizosaccharomyces pombe... 25 9.9
>SPAC6G10.08 |idp1||isocitrate dehydrogenase
Idp1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 418
Score = 294 bits (721), Expect = 1e-80
Identities = 135/208 (64%), Positives = 161/208 (77%)
Frame = -1
Query: 786 QVCLDRKYPLYLSTKNTILKKYDGRFKDIFQDIYDREYKKQFEDAGIWYEHRLIDDMVAY 607
Q+ L +K PLYLSTKNTILKKYDGRFKD FQ++Y+ +YK++FE+ G+WY+HRLIDDMVA
Sbjct: 208 QMALQKKMPLYLSTKNTILKKYDGRFKDTFQEVYESDYKQKFEELGLWYQHRLIDDMVAQ 267
Query: 606 AMKSEGGFVWACKNYDGDVQSDSVAQGYGSLGLMTSVLICPDGKTVEAEAAHGTVTRHFR 427
A+KS GGFVWACKNYDGDV SD VAQ YGSLGLMTSVLI P+G+T E+EAAHGTV RH+
Sbjct: 268 AIKSNGGFVWACKNYDGDVMSDVVAQAYGSLGLMTSVLIHPNGRTFESEAAHGTVQRHYM 327
Query: 426 FYQQGKETSTNPIASIFAWTRGLLHRAKLDNNDALKNFAETLEKVCIETIESGIMTKDLA 247
Y +GK+TSTN IASIFAWTRGL HR +LD N+ L FA LE C+ +E GIMTKDL
Sbjct: 328 QYLKGKKTSTNSIASIFAWTRGLAHRGRLDGNERLVKFANALEHACVRCVEKGIMTKDLY 387
Query: 246 ICIKGMNNVKRSDYYETFEFMDKLAENL 163
+ K N Y +TFEF+D + L
Sbjct: 388 LLSKSPNG-----YVDTFEFLDAVKSEL 410
>SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 362
Score = 31.9 bits (69), Expect = 0.11
Identities = 23/71 (32%), Positives = 33/71 (46%), Gaps = 1/71 (1%)
Frame = -1
Query: 654 AGIWYEHRLIDDMVAYAMKSEGGF-VWACKNYDGDVQSDSVAQGYGSLGLMTSVLICPDG 478
A I + +++D MV + F V N GD+ SD A GSLGL+ S +
Sbjct: 222 ASINVDEQIVDSMVYRLFREPECFDVVVAPNLYGDILSDGAASLIGSLGLVPSANV--GD 279
Query: 477 KTVEAEAAHGT 445
V +E HG+
Sbjct: 280 NFVMSEPVHGS 290
>SPBC902.05c |idh2|glu2|isocitrate dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 378
Score = 28.3 bits (60), Expect = 1.4
Identities = 51/185 (27%), Positives = 77/185 (41%), Gaps = 6/185 (3%)
Frame = -1
Query: 744 KNTILKKYDGRF----KDIFQDIYDREYKKQFEDAGIWYEHRLIDDMVAYAMKSEGGFVW 577
K TI++ DG F K++ + D E +++ D +++ D V Y V
Sbjct: 213 KATIMRMADGLFLECAKELAPEYPDIELREEILDNACL---KIVTDPVPY-----NNTVM 264
Query: 576 ACKNYDGDVQSDSVAQGYGSLGLMTSVLICPDGKTVEAEAAHGTVTRHFRFYQQGKETST 397
N GD+ SD A G LGL S I + EA HGT GK +
Sbjct: 265 VMPNLYGDIVSDMCAGLIGGLGLTPSGNI--GNQASIFEAVHGTAPD-----IAGKGLA- 316
Query: 396 NPIASIFAWTRGLLHRAKLDNNDALKNFAETLEKVCIETIESG--IMTKDLAICIKGMNN 223
NP A + + L H ++ ND +A+ +E +T+ + TKDL NN
Sbjct: 317 NPTALLLSSVMMLKH---MNLND----YAKRIESAIFDTLANNPDARTKDLG---GKSNN 366
Query: 222 VKRSD 208
V+ +D
Sbjct: 367 VQYTD 371
>SPBC16A3.13 |meu7|aah4|alpha-amylase homolog
Aah4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 774
Score = 27.5 bits (58), Expect = 2.4
Identities = 19/57 (33%), Positives = 25/57 (43%), Gaps = 1/57 (1%)
Frame = +3
Query: 522 HSPEQHYQIVRLHHNFCRPRQIHPLIS*HKPPYHQLACVHTRYQHPQTVSCT-PCHK 689
H P +H + H F RP + HKP H+ C H R+ P + T P HK
Sbjct: 278 HKPWKHEEHCH-HGKFPRPIPHNGTKPDHKPWKHEEHCHHGRFPRPVPHNGTKPDHK 333
Score = 27.1 bits (57), Expect = 3.2
Identities = 18/57 (31%), Positives = 25/57 (43%), Gaps = 1/57 (1%)
Frame = +3
Query: 522 HSPEQHYQIVRLHHNFCRPRQIHPLIS*HKPPYHQLACVHTRYQHPQTVSCT-PCHK 689
H P +H + H F RP + HKP H+ C H ++ P + T P HK
Sbjct: 361 HKPWKHEEHCH-HGKFLRPVPHNVTKPDHKPWKHEEHCHHGKFPRPVPHNGTKPDHK 416
>SPBC16H5.10c |prp43||ATP-dependent RNA helicase
Prp43|Schizosaccharomyces pombe|chr 2|||Manual
Length = 735
Score = 27.1 bits (57), Expect = 3.2
Identities = 17/73 (23%), Positives = 33/73 (45%)
Frame = -1
Query: 453 HGTVTRHFRFYQQGKETSTNPIASIFAWTRGLLHRAKLDNNDALKNFAETLEKVCIETIE 274
H T+ + Y+ G+ T+ + W L HRA + ++ K T+E+ +E I
Sbjct: 561 HLTLLNVYHAYKSGEGTAD------WCWNHFLSHRALISADNVRKQLRRTMERQEVELIS 614
Query: 273 SGIMTKDLAICIK 235
+ K+ + I+
Sbjct: 615 TPFDDKNYYVNIR 627
>SPBC27.01c |||secretory pathway protein Pga2 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 132
Score = 26.6 bits (56), Expect = 4.3
Identities = 18/57 (31%), Positives = 24/57 (42%), Gaps = 1/57 (1%)
Frame = -1
Query: 483 DGKTVEAEAAHGTVTR-HFRFYQQGKETSTNPIASIFAWTRGLLHRAKLDNNDALKN 316
DG T++ E HGT + H F +E NP A F W R + + KN
Sbjct: 56 DG-TLDPEMTHGTKPKEHGEFDTDDEEEEENPDAE-FRWGYSARRRIRKQREEYFKN 110
>SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1029
Score = 26.6 bits (56), Expect = 4.3
Identities = 17/63 (26%), Positives = 29/63 (46%), Gaps = 4/63 (6%)
Frame = -1
Query: 645 WYEH-RLIDDMVAYAMKSEGGFVWACKNYDGDVQSD---SVAQGYGSLGLMTSVLICPDG 478
W H DD+ + G V+ KNYDGDV++D + + G+ + + P+G
Sbjct: 794 WLGHPSYFDDLTSLDPNLYEGLVFL-KNYDGDVENDMALNFTVVHEEFGVRNVIDLIPNG 852
Query: 477 KTV 469
+
Sbjct: 853 SNI 855
>SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase
Cdc25|Schizosaccharomyces pombe|chr 1|||Manual
Length = 596
Score = 26.2 bits (55), Expect = 5.6
Identities = 12/33 (36%), Positives = 19/33 (57%)
Frame = -1
Query: 747 TKNTILKKYDGRFKDIFQDIYDREYKKQFEDAG 649
T+ T+L DG+FKDIF + + ++E G
Sbjct: 414 TQETLLGLLDGKFKDIFDKCIIIDCRFEYEYLG 446
>SPAC22A12.11 |dak1||dihydroxyacetone kinase
Dak1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 580
Score = 25.4 bits (53), Expect = 9.9
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = -1
Query: 603 MKSEGGFVWACKNYDGDV 550
++SE G + CKNY GD+
Sbjct: 93 VESEAGTLVICKNYTGDI 110
>SPAC926.02 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 443
Score = 25.4 bits (53), Expect = 9.9
Identities = 15/31 (48%), Positives = 18/31 (58%), Gaps = 3/31 (9%)
Frame = -1
Query: 339 DNNDALKNFAETLEKVCIETIESG---IMTK 256
D DA +FAETL C+ T ES IMT+
Sbjct: 301 DKADAYIDFAETLLDSCVSTEESASIEIMTR 331
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,261,035
Number of Sequences: 5004
Number of extensions: 67278
Number of successful extensions: 175
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 159
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 169
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 404442380
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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