BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= rbmte14a08
(809 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1259.12c |||Ran GTPase binding protein |Schizosaccharomyces ... 28 1.8
SPAC23H4.08 |||RNA polymerase II associated protein |Schizosacch... 27 3.2
SPAC9.05 |mfh1||ATP-dependent DNA helicase Mfh1 |Schizosaccharom... 27 3.2
SPAC6F6.01 |||VIC sodium channel |Schizosaccharomyces pombe|chr ... 27 4.2
SPBC2G2.12 |||histidine-tRNA ligase|Schizosaccharomyces pombe|ch... 27 4.2
SPAC644.06c |cdr1|nim1|GIN4 family protein kinase Cdr1|Schizosac... 27 4.2
SPAC3A12.15 |vps53||GARP complex subunit Vps53 |Schizosaccharomy... 26 5.5
SPAC1071.01c |pta1|SPAC4H3.15c|mRNA cleavage and polyadenylation... 26 7.3
SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein lig... 25 9.6
SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces pombe... 25 9.6
SPBC26H8.08c |grn1||GTPase Grn1 |Schizosaccharomyces pombe|chr 2... 25 9.6
>SPCC1259.12c |||Ran GTPase binding protein |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 486
Score = 27.9 bits (59), Expect = 1.8
Identities = 18/61 (29%), Positives = 30/61 (49%)
Frame = -2
Query: 799 FDVPHAIVCESNKILKFSVRQIKMLVQTLSTLMYTFVFLSLFLVDYLLGNGFVDMFQTIF 620
+D+ +AI E NK+ + + + K L FL+ + +LL NGFV+ + F
Sbjct: 235 YDIDYAIQMEKNKLFEQATKSPKQ-----EELKQRQEFLNELISSFLLNNGFVETAKK-F 288
Query: 619 C 617
C
Sbjct: 289 C 289
>SPAC23H4.08 |||RNA polymerase II associated protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 277
Score = 27.1 bits (57), Expect = 3.2
Identities = 25/115 (21%), Positives = 49/115 (42%)
Frame = -2
Query: 673 LVDYLLGNGFVDMFQTIFCKIACFVKKTLREEAKKTQLVGKENPISIPVIFIEILVLSLT 494
++ L NG + + Q+ + + + +E K T G + +SIP F L L
Sbjct: 161 MIQEYLSNGDLPLQQSTEDYVYDIYEASSKEPNKPTFAYGVIDALSIPDAFRSSLEQELV 220
Query: 493 IAFLNKYKKYRAKDRIDELLNESKEAIRQTNEFLEKWRLRRIPTDFSYLDEEPQE 329
++ K D IDE N ++ + + ++W+ +F+Y D+ Q+
Sbjct: 221 SETVDSDKDDPLHDEIDEDSN-AESFYQNSYPDEDEWQDSSENDEFAYSDDAEQD 274
>SPAC9.05 |mfh1||ATP-dependent DNA helicase Mfh1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 834
Score = 27.1 bits (57), Expect = 3.2
Identities = 30/92 (32%), Positives = 39/92 (42%), Gaps = 1/92 (1%)
Frame = +3
Query: 507 TSISMNMTGMLIGFSLPTNCVFFASSRNVFF-TKQAILQNIVWNISTKPLPNK*STRNKL 683
T I + T L G T + SRNVFF T Q IL +I I + +
Sbjct: 140 TGIPKSQTAELSGHVPVTTRNQYYQSRNVFFVTPQTILNDIKHGICDRTRISCLVIDEAH 199
Query: 684 KKTKVYIKVLNVCTSILIWRTENFKILLLSHT 779
+ T Y V V +L +NF+IL LS T
Sbjct: 200 RSTGNYAYVEVV--HLLSLSNKNFRILALSAT 229
>SPAC6F6.01 |||VIC sodium channel |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1854
Score = 26.6 bits (56), Expect = 4.2
Identities = 14/41 (34%), Positives = 21/41 (51%)
Frame = +2
Query: 509 QYFDEYDRNANRILLANQLRFFRLLSQCFLHETSYLAEYRL 631
Q +D+ ANR L+ F L ++ L +YL +YRL
Sbjct: 536 QTYDQTSSTANRYLIFYACVDFLLAAEVILRFFAYLPDYRL 576
>SPBC2G2.12 |||histidine-tRNA ligase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 538
Score = 26.6 bits (56), Expect = 4.2
Identities = 11/22 (50%), Positives = 15/22 (68%)
Frame = -2
Query: 805 DKFDVPHAIVCESNKILKFSVR 740
DKFDVP A++ ++ K SVR
Sbjct: 485 DKFDVPFAVILGQDEFAKGSVR 506
>SPAC644.06c |cdr1|nim1|GIN4 family protein kinase
Cdr1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 593
Score = 26.6 bits (56), Expect = 4.2
Identities = 16/50 (32%), Positives = 24/50 (48%), Gaps = 1/50 (2%)
Frame = +1
Query: 34 HLLRPHPTRIDY*TRYFSICPKIDYPYRCD*IRL-SIYHQSDPVSILGHL 180
H + +P+ +D Y +CP +Y Y D +R S+ QS P L L
Sbjct: 417 HEVVSNPSTLDDDYNYMFVCPPEEYTYSTDNVRTDSLDLQSLPTPTLEQL 466
>SPAC3A12.15 |vps53||GARP complex subunit Vps53 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 756
Score = 26.2 bits (55), Expect = 5.5
Identities = 12/32 (37%), Positives = 18/32 (56%)
Frame = -2
Query: 514 ILVLSLTIAFLNKYKKYRAKDRIDELLNESKE 419
I ++ T+ LN +KKYR+ +RI L E
Sbjct: 152 ISLMQATLQLLNFFKKYRSVERIASLSRSISE 183
>SPAC1071.01c |pta1|SPAC4H3.15c|mRNA cleavage and polyadenylation
specificity factor complex subunit
Pta1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 670
Score = 25.8 bits (54), Expect = 7.3
Identities = 13/40 (32%), Positives = 21/40 (52%)
Frame = -2
Query: 391 EKWRLRRIPTDFSYLDEEPQEIKPLKLEVPILHMAIIDTL 272
EKW L+ I + YL+ + + E+P L AI+D +
Sbjct: 603 EKWALKVIDSILPYLEAKDKVFMIFMSELPELTDAIVDKI 642
>SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein ligase
E3 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1647
Score = 25.4 bits (53), Expect = 9.6
Identities = 11/34 (32%), Positives = 22/34 (64%), Gaps = 3/34 (8%)
Frame = -1
Query: 416 HTSDKRVSREMATA---EDTYRFFLLGRRTPRNQ 324
HT++ R+ +T+ E+ + F L+G++ PRN+
Sbjct: 1063 HTTEPSERRDSSTSSHFEEHFVFSLMGKKVPRNK 1096
>SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 560
Score = 25.4 bits (53), Expect = 9.6
Identities = 10/28 (35%), Positives = 15/28 (53%)
Frame = -2
Query: 400 EFLEKWRLRRIPTDFSYLDEEPQEIKPL 317
EF+ +W R+IP + D+ P PL
Sbjct: 358 EFIGEWYPRKIPNPDYFDDDHPSHFGPL 385
>SPBC26H8.08c |grn1||GTPase Grn1 |Schizosaccharomyces pombe|chr
2|||Manual
Length = 470
Score = 25.4 bits (53), Expect = 9.6
Identities = 17/61 (27%), Positives = 30/61 (49%), Gaps = 5/61 (8%)
Frame = -2
Query: 481 NKYKKYRAKDRIDELLNESKEAIRQTNEFLEKWRLR-----RIPTDFSYLDEEPQEIKPL 317
+K + R + RI++ ESK ++ ++ +W+ R IP F Y D+ EI+
Sbjct: 8 SKRRTTRLRSRIEKKAAESKRKQKRADKKNPQWKSRIPKDPGIPNSFPYKDKILAEIEEQ 67
Query: 316 K 314
K
Sbjct: 68 K 68
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,354,227
Number of Sequences: 5004
Number of extensions: 72052
Number of successful extensions: 237
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 226
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 237
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 394431430
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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