BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= rbmte14a05
(756 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.16c |||P-type ATPase |Schizosaccharomyces pombe|chr 1||... 31 0.13
SPCC550.11 |||karyopherin|Schizosaccharomyces pombe|chr 3|||Manual 28 1.7
SPAC19G12.07c |rsd1||RNA-binding protein Rsd1|Schizosaccharomyce... 28 1.7
SPAC144.06 |apl5||AP-3 adaptor complex subunit Apl5 |Schizosacch... 28 1.7
SPBC106.04 |ada1||adenosine deaminase Ada1 |Schizosaccharomyces ... 27 3.8
SPAC57A7.05 |||conserved protein |Schizosaccharomyces pombe|chr ... 27 3.8
SPBC32H8.03 |bem46||esterase/lipase |Schizosaccharomyces pombe|c... 26 5.0
SPAC25B8.08 |||conserved fungal family|Schizosaccharomyces pombe... 26 5.0
SPBC21C3.20c |git1||C2 domain protein Git1|Schizosaccharomyces p... 26 5.0
SPAC2E1P5.01c |mns1|SPAPB1E7.13c|mannosyl-oligosaccharide 1,2-al... 26 6.7
SPAC212.08c |||GPI anchored protein |Schizosaccharomyces pombe|c... 26 6.7
SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein lig... 25 8.8
SPBP8B7.21 |ubp3||ubiquitin C-terminal hydrolase Ubp3|Schizosacc... 25 8.8
SPAC31F12.01 |zds1|SPAC637.14, mug88|zds family protein Zds1|Sch... 25 8.8
>SPAC4F10.16c |||P-type ATPase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1367
Score = 31.5 bits (68), Expect = 0.13
Identities = 17/52 (32%), Positives = 27/52 (51%)
Frame = -1
Query: 645 CINFPRNLNYDSPSSCSVTCGRNLNIEFRSIVDNFLIRLGTAVAANFVSNLE 490
CI RNLNY+ SC V + +E S+ D+ T +++ FV +L+
Sbjct: 631 CIKKRRNLNYNENLSCKVDLDKKKMLETLSLSDSPNPESITFISSKFVDHLQ 682
>SPCC550.11 |||karyopherin|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1029
Score = 27.9 bits (59), Expect = 1.7
Identities = 16/44 (36%), Positives = 24/44 (54%), Gaps = 1/44 (2%)
Frame = -1
Query: 393 HPLSKLVSWLP-YFKNTFVKPFTDSPDYSFTTDEYLAVVPDLPI 265
H L+ S L +NTFVK ++ D S T D++ ++V D I
Sbjct: 578 HELTPFASQLAKQLRNTFVKLMQETMDESTTVDDFDSLVDDKSI 621
>SPAC19G12.07c |rsd1||RNA-binding protein Rsd1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 604
Score = 27.9 bits (59), Expect = 1.7
Identities = 25/81 (30%), Positives = 32/81 (39%)
Frame = +3
Query: 216 PPTHRERLCPLSNVASKSEGPEPRRDTHQS*TNSRENR*TVLRTYS*SRAARKPVSIEDV 395
P HR P S+ S+S P D+HQS + R S S +R P S
Sbjct: 113 PRHHRRSYSPRSDYGSRSPSPHSSVDSHQSRSPVRSRD---RDRSSRSSRSRHPSSRS-- 167
Query: 396 WRFRPSNY*REPPRPAHQEAS 458
R R +Y R PP + S
Sbjct: 168 -RHRYDDYSRSPPYSSRHSRS 187
>SPAC144.06 |apl5||AP-3 adaptor complex subunit Apl5
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 834
Score = 27.9 bits (59), Expect = 1.7
Identities = 13/34 (38%), Positives = 21/34 (61%)
Frame = +2
Query: 533 LMRKLSTIERNSMLRFLPHVTLQLDGLS*FKFLG 634
LM + ++++ M +P +TL +DGLS F LG
Sbjct: 98 LMLTTNLLKKDLMSSKVPEITLAIDGLSHFSTLG 131
>SPBC106.04 |ada1||adenosine deaminase Ada1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 846
Score = 26.6 bits (56), Expect = 3.8
Identities = 15/38 (39%), Positives = 20/38 (52%)
Frame = -1
Query: 402 IAKHPLSKLVSWLPYFKNTFVKPFTDSPDYSFTTDEYL 289
IA PLS +L Y KN F+ F + S +TD+ L
Sbjct: 645 IAMSPLSNNALFLAYDKNPFLTYFKRGLNVSLSTDDPL 682
>SPAC57A7.05 |||conserved protein |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1337
Score = 26.6 bits (56), Expect = 3.8
Identities = 15/40 (37%), Positives = 24/40 (60%), Gaps = 3/40 (7%)
Frame = -1
Query: 633 PRNLNYDSPSSCSVTCGRNLNIEFRSI---VDNFLIRLGT 523
PR+ + D PSS CG L F+SI ++ F++++GT
Sbjct: 887 PRDPDADHPSSLYHICGYRLTKFFKSIFRPMNVFVLKVGT 926
>SPBC32H8.03 |bem46||esterase/lipase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 299
Score = 26.2 bits (55), Expect = 5.0
Identities = 30/124 (24%), Positives = 52/124 (41%)
Frame = -1
Query: 444 ALVLEAPFNNLKDEIAKHPLSKLVSWLPYFKNTFVKPFTDSPDYSFTTDEYLAVVPDLPI 265
AL+LE F ++KD I + PY + + T+ +++ + + + LP+
Sbjct: 187 ALILENTFTSIKDMIP--------TVFPYGGSIISRFCTEI----WSSQDEIRKIKKLPV 234
Query: 264 LMLHSKGDRVVPYELAVKLQNKLVQSRKSSSAPFTFQTFEREHSIGHNNLCEAKHFKSAI 85
L L + D +VP V L ++K F +F + HN+ C + I
Sbjct: 235 LFLSGEKDEIVPPPQMVLLFGLCGSAKKK------FHSFPK---CTHNDTCLGDGYFQVI 285
Query: 84 TDFL 73
DFL
Sbjct: 286 ADFL 289
>SPAC25B8.08 |||conserved fungal family|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 590
Score = 26.2 bits (55), Expect = 5.0
Identities = 15/47 (31%), Positives = 22/47 (46%)
Frame = -1
Query: 525 TAVAANFVSNLEELCQERGSEKLRLPDALVLEAPFNNLKDEIAKHPL 385
T V+ F+ L+ +E+ EK+ D + F LKDE PL
Sbjct: 465 TKVSKTFLKKLDAFHEEKLREKINSSDKGDVSYEFELLKDETVFSPL 511
>SPBC21C3.20c |git1||C2 domain protein Git1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1098
Score = 26.2 bits (55), Expect = 5.0
Identities = 15/40 (37%), Positives = 21/40 (52%), Gaps = 1/40 (2%)
Frame = -1
Query: 396 KHPLSKLVSWLPYFKNTFVKPFTDSPDYSF-TTDEYLAVV 280
K L + SWL F + F + F D + F TDEY +V+
Sbjct: 1042 KRELKMVYSWLQLFYD-FFRNFQDIAPFDFLNTDEYKSVM 1080
>SPAC2E1P5.01c |mns1|SPAPB1E7.13c|mannosyl-oligosaccharide
1,2-alpha-mannosidase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 521
Score = 25.8 bits (54), Expect = 6.7
Identities = 9/25 (36%), Positives = 15/25 (60%)
Frame = -1
Query: 354 KNTFVKPFTDSPDYSFTTDEYLAVV 280
K TF++ +TD Y + DEY ++
Sbjct: 51 KKTFIESWTDYETYGWGKDEYYPII 75
>SPAC212.08c |||GPI anchored protein |Schizosaccharomyces pombe|chr
1|||Manual
Length = 278
Score = 25.8 bits (54), Expect = 6.7
Identities = 10/21 (47%), Positives = 13/21 (61%)
Frame = +1
Query: 649 REYHFPLDRFIVSEHQGQHGN 711
R Y +P D F+VS + Q GN
Sbjct: 192 RSYFYPQDSFLVSHAEWQDGN 212
>SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein ligase
E3 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1647
Score = 25.4 bits (53), Expect = 8.8
Identities = 20/58 (34%), Positives = 30/58 (51%), Gaps = 2/58 (3%)
Frame = -1
Query: 615 DSPSSCSVTCGRNLNI--EFRSIVDNFLIRLGTAVAANFVSNLEELCQERGSEKLRLP 448
+ PSS SV GR+LN+ +F +++ L T V +F L+ Q S+K LP
Sbjct: 70 EPPSSKSVEFGRHLNMRHDFSELLEQPLTTSKTRVFKSFNEKLKGKPQLVKSDKGVLP 127
>SPBP8B7.21 |ubp3||ubiquitin C-terminal hydrolase
Ubp3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 512
Score = 25.4 bits (53), Expect = 8.8
Identities = 19/73 (26%), Positives = 33/73 (45%), Gaps = 2/73 (2%)
Frame = -1
Query: 459 LRLPDAL--VLEAPFNNLKDEIAKHPLSKLVSWLPYFKNTFVKPFTDSPDYSFTTDEYLA 286
LR+P A VL PF L+ +I + ++ L + + P S + T + +
Sbjct: 322 LRVPSARDSVLLEPFQPLQLDIQAEDIHSVIDALEHMTAPEILPEWHSSKGNVTATKQMY 381
Query: 285 VVPDLPILMLHSK 247
+ P+L+LH K
Sbjct: 382 IESLPPVLILHLK 394
>SPAC31F12.01 |zds1|SPAC637.14, mug88|zds family protein
Zds1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 938
Score = 25.4 bits (53), Expect = 8.8
Identities = 16/33 (48%), Positives = 21/33 (63%), Gaps = 3/33 (9%)
Frame = -2
Query: 632 RET*TTIARLVAAL---HAGETSTLSSVRSWTI 543
R + TTI R A++ H G+TSTLS RS +I
Sbjct: 309 RSSRTTIRRTGASIRTIHRGKTSTLSGNRSHSI 341
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,021,762
Number of Sequences: 5004
Number of extensions: 63655
Number of successful extensions: 227
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 213
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 227
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 361294920
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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