Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= rbmte13k19
(784 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 58 1e-09
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 44 2e-05
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 39 0.001
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 37 0.003
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 37 0.004
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 35 0.011
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 35 0.011
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 30 0.43
SPCC4B3.13 |||MatE family transporter|Schizosaccharomyces pombe|... 26 7.0
SPAC22G7.04 |ubp13|pan2|poly|Schizosaccharomyces pombe|chr 1|||M... 26 7.0
SPAC18G6.04c |shm2||serine hydroxymethyltransferase Shm2 |Schizo... 25 9.3
SPBC30D10.10c |tor1||phosphatidylinositol kinase Tor1|Schizosacc... 25 9.3
SPBC17A3.01c |tim50|SPBC8D2.21c|TIM23 translocase complex subuni... 25 9.3
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 58.4 bits (135), Expect = 1e-09
Identities = 42/132 (31%), Positives = 68/132 (51%), Gaps = 8/132 (6%)
Frame = -1
Query: 562 QVQQWFRAVDKDQSGFITSTELRSALVNAQGQTFSETACNLMIGMFDKDRNGQINLDEF- 386
+ ++ F D+DQ G ITS EL ++ + GQ+ + MI D D NG I+ EF
Sbjct: 13 EFREAFSLFDRDQDGNITSNEL-GVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFL 71
Query: 385 ----DKLYTYVN--QWLAVFKTYDTDQSGFIDEGELSKALTQMGFRFSPEFINILTKRSN 224
K+ N + FK +D D +G+I EL+ LT +G R S E + + + ++
Sbjct: 72 TMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREAD 131
Query: 223 TN-DGKISVDQF 191
T+ DG I+ ++F
Sbjct: 132 TDGDGVINYEEF 143
Score = 40.3 bits (90), Expect = 3e-04
Identities = 22/62 (35%), Positives = 35/62 (56%)
Frame = -1
Query: 562 QVQQWFRAVDKDQSGFITSTELRSALVNAQGQTFSETACNLMIGMFDKDRNGQINLDEFD 383
+V++ F+ DKD +G+IT EL L + G+ S+ MI D D +G IN +EF
Sbjct: 86 EVREAFKVFDKDGNGYITVEELTHVLTSL-GERLSQEEVADMIREADTDGDGVINYEEFS 144
Query: 382 KL 377
++
Sbjct: 145 RV 146
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 44.0 bits (99), Expect = 2e-05
Identities = 34/128 (26%), Positives = 58/128 (45%), Gaps = 3/128 (2%)
Frame = -1
Query: 562 QVQQWFRAVDKDQSGFITSTELRSALVNAQGQTFSETACNLMIGMFDKDRNGQINLDEFD 383
++++ F +DKD G I ++++ L + Q SE + N M + N L
Sbjct: 49 ELKEAFALLDKDGDGNIGREDVKTMLTSLN-QDASEDSINHMFESINPPINLAAFLTAMG 107
Query: 382 KLYTYV---NQWLAVFKTYDTDQSGFIDEGELSKALTQMGFRFSPEFINILTKRSNTNDG 212
+ + N L F T+D QSG I + AL+ MG R P+ + + RS T+ G
Sbjct: 108 SMLCRISPRNDLLEAFSTFDDTQSGKIPISTMRDALSSMGDRMDPQEVESIL-RSYTSHG 166
Query: 211 KISVDQFI 188
++F+
Sbjct: 167 VFYYEKFV 174
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 38.7 bits (86), Expect = 0.001
Identities = 19/55 (34%), Positives = 29/55 (52%)
Frame = -1
Query: 346 FKTYDTDQSGFIDEGELSKALTQMGFRFSPEFINILTKRSNTNDGKISVDQFIVM 182
F+ +D D +G I GEL LT +G + S E ++ L K DG ++ F+ M
Sbjct: 83 FQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGVPVKDGMVNYHDFVQM 137
Score = 28.3 bits (60), Expect = 1.3
Identities = 18/54 (33%), Positives = 27/54 (50%)
Frame = -1
Query: 547 FRAVDKDQSGFITSTELRSALVNAQGQTFSETACNLMIGMFDKDRNGQINLDEF 386
F+ DKD +G I ELR L + + +E L+ G+ KD G +N +F
Sbjct: 83 FQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGVPVKD--GMVNYHDF 134
>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 174
Score = 37.1 bits (82), Expect = 0.003
Identities = 35/137 (25%), Positives = 59/137 (43%), Gaps = 13/137 (9%)
Frame = -1
Query: 562 QVQQWFRAVDKDQSGFITSTELRSALVNAQGQTFSETACNLMIGMFDKDRNGQINLDEF- 386
++++ F +D +QSG I E S A S + + + D+D G ++ EF
Sbjct: 25 RIRKRFIKIDANQSGSIDRNEFLSIPSVA-----SNPLASRLFSVVDEDGGGDVDFQEFI 79
Query: 385 DKLYTYV------NQWLAVFKTYDTDQSGFIDEGELSKALTQM-GFRFSPEFINILTKRS 227
+ L + + FK YD D+ G+I GEL L M G + + + ++
Sbjct: 80 NSLSVFSVHGNKEEKLKFAFKIYDIDRDGYISNGELYLVLKMMVGTNLREDQLQQIVDKT 139
Query: 226 -----NTNDGKISVDQF 191
DGKIS ++F
Sbjct: 140 IMEVDKDRDGKISFEEF 156
Score = 31.5 bits (68), Expect = 0.14
Identities = 19/66 (28%), Positives = 32/66 (48%), Gaps = 4/66 (6%)
Frame = -1
Query: 547 FRAVDKDQSGFITSTELRSALVNAQGQTFSETACNLMIGM----FDKDRNGQINLDEFDK 380
F+ D D+ G+I++ EL L G E ++ DKDR+G+I+ +EF
Sbjct: 99 FKIYDIDRDGYISNGELYLVLKMMVGTNLREDQLQQIVDKTIMEVDKDRDGKISFEEFKD 158
Query: 379 LYTYVN 362
+ + N
Sbjct: 159 IVSGSN 164
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 36.7 bits (81), Expect = 0.004
Identities = 24/87 (27%), Positives = 42/87 (48%), Gaps = 8/87 (9%)
Frame = -1
Query: 346 FKTYDTDQSGFIDEGELSKALTQMGFRF-SPEFINILTKRSNTNDGKISVDQFI-VMCVQ 173
FK +D+D+ ID EL A+ +GF E + IL T G + ++ F+ VM +
Sbjct: 43 FKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQMEDFVRVMTEK 102
Query: 172 I------QRFTEAFRVRDTEQNGTVTI 110
I + AF + D ++ G +++
Sbjct: 103 IVERDPLEEIKRAFELFDDDETGKISL 129
>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 190
Score = 35.1 bits (77), Expect = 0.011
Identities = 25/98 (25%), Positives = 46/98 (46%), Gaps = 9/98 (9%)
Frame = -1
Query: 562 QVQQWFRAVDKD-QSGFITSTELRSALVNAQGQTFSETAC--NLMIGMFDKDRNGQINLD 392
++QQW++ KD SG + +E + + Q F + + + +FD D+NG I+
Sbjct: 26 ELQQWYKGFFKDCPSGHLNKSEFQK--IYKQFFPFGDPSAFAEYVFNVFDADKNGYIDFK 83
Query: 391 EFDKLYTYV------NQWLAVFKTYDTDQSGFIDEGEL 296
EF + ++ + F+ YD D +G I E+
Sbjct: 84 EFICALSVTSRGELNDKLIWAFQLYDLDNNGLISYDEM 121
Score = 29.5 bits (63), Expect = 0.57
Identities = 26/91 (28%), Positives = 39/91 (42%), Gaps = 9/91 (9%)
Frame = -1
Query: 433 GMFDKDRNGQINLDEFDKLYTYVNQW-------LAVFKTYDTDQSGFIDEGELSKAL--T 281
G F +G +N EF K+Y + VF +D D++G+ID E AL T
Sbjct: 33 GFFKDCPSGHLNKSEFQKIYKQFFPFGDPSAFAEYVFNVFDADKNGYIDFKEFICALSVT 92
Query: 280 QMGFRFSPEFINILTKRSNTNDGKISVDQFI 188
G + + I N+G IS D+ +
Sbjct: 93 SRG-ELNDKLIWAFQLYDLDNNGLISYDEML 122
Score = 27.1 bits (57), Expect = 3.0
Identities = 10/24 (41%), Positives = 16/24 (66%)
Frame = -1
Query: 457 ETACNLMIGMFDKDRNGQINLDEF 386
E N + M DK+++GQ+ L+EF
Sbjct: 146 EKRVNKIFNMMDKNKDGQLTLEEF 169
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 35.1 bits (77), Expect = 0.011
Identities = 16/59 (27%), Positives = 32/59 (54%)
Frame = -1
Query: 346 FKTYDTDQSGFIDEGELSKALTQMGFRFSPEFINILTKRSNTNDGKISVDQFIVMCVQI 170
F +D G+ID +L ++ Q+G + E + ++ + TN GK+S ++F + + I
Sbjct: 18 FDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLMLDLAGTN-GKVSREEFAELWIHI 75
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 29.9 bits (64), Expect = 0.43
Identities = 15/54 (27%), Positives = 26/54 (48%)
Frame = -1
Query: 547 FRAVDKDQSGFITSTELRSALVNAQGQTFSETACNLMIGMFDKDRNGQINLDEF 386
FR DKD SG+I + + + + G+ S+ LM+ D +G + +F
Sbjct: 84 FRVFDKDNSGYIETAKF-ADYMKTLGEKLSDNEVQLMVQEADPTNSGSFDYYDF 136
>SPCC4B3.13 |||MatE family transporter|Schizosaccharomyces pombe|chr
3|||Manual
Length = 539
Score = 25.8 bits (54), Expect = 7.0
Identities = 20/66 (30%), Positives = 29/66 (43%)
Frame = -1
Query: 655 GQAAYGGMPPGQLEIGHGPYPSIGVGGTITPQVQQWFRAVDKDQSGFITSTELRSALVNA 476
G+ A+GG L+ GP + V G I + W + SG + +TEL S V +
Sbjct: 304 GRQAWGGFSREALK-NWGPLCRLAVPGVIMICSEYWAFELVTFASGVLGTTELASMSVLS 362
Query: 475 QGQTFS 458
T S
Sbjct: 363 TTSTLS 368
>SPAC22G7.04 |ubp13|pan2|poly|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1115
Score = 25.8 bits (54), Expect = 7.0
Identities = 12/19 (63%), Positives = 13/19 (68%)
Frame = +2
Query: 416 VFVEHTNHQIASSFGKSLT 472
V+ H N QI SSFG SLT
Sbjct: 33 VWTGHKNGQIKSSFGPSLT 51
>SPAC18G6.04c |shm2||serine hydroxymethyltransferase Shm2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 472
Score = 25.4 bits (53), Expect = 9.3
Identities = 9/17 (52%), Positives = 11/17 (64%)
Frame = -1
Query: 685 LQMAYNSGYPGQAAYGG 635
+Q Y+ GYPG YGG
Sbjct: 61 MQNKYSEGYPGARYYGG 77
>SPBC30D10.10c |tor1||phosphatidylinositol kinase
Tor1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2335
Score = 25.4 bits (53), Expect = 9.3
Identities = 8/22 (36%), Positives = 16/22 (72%)
Frame = +1
Query: 415 GLCRTYQSSDCKQFRKKFDLER 480
GLC T ++D + F+++ ++ER
Sbjct: 2002 GLCNTLLTTDSETFKRRLNIER 2023
>SPBC17A3.01c |tim50|SPBC8D2.21c|TIM23 translocase complex subunit
Tim50 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 452
Score = 25.4 bits (53), Expect = 9.3
Identities = 10/34 (29%), Positives = 19/34 (55%)
Frame = +1
Query: 589 WKGTVHVQFLIGLVAFHRKLPVLDIRNCRPFVVS 690
W G + L+GL+ + ++DI++ RP + S
Sbjct: 297 WTGNPKDKELVGLIPLLEFIAIMDIKDVRPVLKS 330
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,301,736
Number of Sequences: 5004
Number of extensions: 72362
Number of successful extensions: 180
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 162
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 178
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 379359666
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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