BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= rbmte13k04
(710 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPMIT.06 |||mitochondrial DNA binding endonuclease|Schizosacchar... 28 1.1
SPBC17D11.05 |tif32||translation initiation factor eIF3a|Schizos... 27 2.6
SPBC428.17c |||conserved fungal protein|Schizosaccharomyces pomb... 27 3.5
SPBC530.13 |||cyclin Ctk2|Schizosaccharomyces pombe|chr 2|||Manual 26 6.1
SPAC869.04 |||formamidase-like protein|Schizosaccharomyces pombe... 26 6.1
SPCC18B5.03 |wee1||dual specificity protein kinase Wee1|Schizosa... 26 6.1
SPAC1B9.03c ||SPAC6B12.01|RNA-binding protein|Schizosaccharomyce... 25 8.1
>SPMIT.06 |||mitochondrial DNA binding
endonuclease|Schizosaccharomyces pombe|chr
mitochondrial|||Manual
Length = 807
Score = 28.3 bits (60), Expect = 1.1
Identities = 20/62 (32%), Positives = 30/62 (48%), Gaps = 5/62 (8%)
Frame = -1
Query: 317 YRKRDDKNKTRLHKQRGRKMSNRNAWSSR----KGNVYTLEA*NVFC*IDYNIRC-ACRG 153
Y RD+ NK +L Q + N W + K N+ TL A ++ N++C AC+
Sbjct: 691 YNSRDENNKPKLITQLFKPSWKVNVWGFKSDKVKLNIRTLYASHLSMANLENLQCAACQS 750
Query: 152 TY 147
TY
Sbjct: 751 TY 752
>SPBC17D11.05 |tif32||translation initiation factor
eIF3a|Schizosaccharomyces pombe|chr 2|||Manual
Length = 932
Score = 27.1 bits (57), Expect = 2.6
Identities = 16/77 (20%), Positives = 37/77 (48%), Gaps = 2/77 (2%)
Frame = -2
Query: 691 EPLVLSPGSMKEKKMISNEN--ILANLLRVKRSISYETIMFANDMREQFKTMIKSMDTPQ 518
EP +PG +++ M N+ L ++ SIS+ + +F+N ++ ++S +
Sbjct: 452 EPYDFNPGQIEKFIMNGNKKGAFSIRLNHIENSISFSSDLFSNPIKSSDSVSLQSTPSEL 511
Query: 517 SQEPLSSIVESTNGYVL 467
L+ I +S + ++
Sbjct: 512 ITSQLTRIAKSLSSVLM 528
>SPBC428.17c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 602
Score = 26.6 bits (56), Expect = 3.5
Identities = 13/42 (30%), Positives = 22/42 (52%)
Frame = -1
Query: 377 SATASIWEFLRITQGRCGEKYRKRDDKNKTRLHKQRGRKMSN 252
S+ + +W FL +T ++ R RLHK++ R +SN
Sbjct: 17 SSESEVWNFLDVTVSELNKQKRSPGQTVSKRLHKKQ-RVVSN 57
>SPBC530.13 |||cyclin Ctk2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 325
Score = 25.8 bits (54), Expect = 6.1
Identities = 19/51 (37%), Positives = 27/51 (52%), Gaps = 3/51 (5%)
Frame = -3
Query: 588 RLLCLRTT*ENNLRL*SRAWI---RRRVKNPFLASWSLRMVTYYD*RVTFP 445
R+L R ENNLR+ + AWI + +K P S L M+ Y ++ FP
Sbjct: 25 RILSTRDPKENNLRMQAFAWISTLSKTLKFPVRTS-GLAMLLYSRFQLFFP 74
>SPAC869.04 |||formamidase-like protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 410
Score = 25.8 bits (54), Expect = 6.1
Identities = 9/19 (47%), Positives = 12/19 (63%)
Frame = +3
Query: 336 LCNSQEFPNTCRRAIQYFR 392
LC + + TC R I+YFR
Sbjct: 327 LCTTTAYRQTCLRVIEYFR 345
>SPCC18B5.03 |wee1||dual specificity protein kinase
Wee1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 877
Score = 25.8 bits (54), Expect = 6.1
Identities = 13/35 (37%), Positives = 19/35 (54%)
Frame = +1
Query: 379 SNIFAKKHCSRKNINWKTISFIGKSYTSIIVRNHS 483
+ + +K+H RKNIN+ + S S VR HS
Sbjct: 383 TGLLSKQHRPRKNINFTPLPPSTPSKPSTFVRPHS 417
>SPAC1B9.03c ||SPAC6B12.01|RNA-binding protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 389
Score = 25.4 bits (53), Expect = 8.1
Identities = 11/31 (35%), Positives = 19/31 (61%)
Frame = -1
Query: 314 RKRDDKNKTRLHKQRGRKMSNRNAWSSRKGN 222
RKR++K + + K+ G SN+N S +G+
Sbjct: 352 RKRENKKRRKDQKKEGITSSNKNDDSGNEGS 382
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,856,137
Number of Sequences: 5004
Number of extensions: 56675
Number of successful extensions: 156
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 138
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 156
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 331187010
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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