Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= rbmte13c14
(812 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 402 e-113
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 200 1e-52
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 199 4e-52
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 129 5e-31
SPBP4H10.04 |ppb1||calcineurin catalytic subunit Ppb1|Schizosacc... 30 0.45
SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizo... 28 1.4
SPBC557.04 |ppk29||Ark1/Prk1 family protein kinase Ppk29|Schizos... 28 1.4
SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizo... 28 1.4
SPBC27B12.05 |||WD repeat protein|Schizosaccharomyces pombe|chr ... 27 4.2
SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein Ubr1|Sc... 26 5.5
SPAC343.10 |met11|mthfr2|methylenetetrahydrofolate reductase Met... 26 5.5
SPBC19C7.12c |||alpha-1,2-mannosyltransferase|Schizosaccharomyce... 26 7.3
SPCP1E11.06 |apl4||AP-1 adaptor complex gamma subunit Apl4 |Schi... 26 7.3
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 402 bits (990), Expect = e-113
Identities = 181/238 (76%), Positives = 213/238 (89%)
Frame = -3
Query: 786 VHQLVENTDESYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNA 607
+HQLVEN+DE++CIDNEAL I TLK+ +P+Y DLNHLVSA M+GVTT RFPG+LN+
Sbjct: 189 MHQLVENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTTSFRFPGELNS 248
Query: 606 DLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHG 427
DLRKLAVNMVPFPRLHFF+ GFAPL + GS ++A++VPELTQQMFDA NMM A DPRHG
Sbjct: 249 DLRKLAVNMVPFPRLHFFMVGFAPLAAIGSSSFQAVSVPELTQQMFDANNMMVAADPRHG 308
Query: 426 RYLTVAAVFRGRMSMKEVDEQMMNIQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFI 247
RYLTVAA+FRG++SMKEVDEQ+ ++Q KNS+YFVEWIP+NV AVC +PP+ LKMSATFI
Sbjct: 309 RYLTVAALFRGKVSMKEVDEQIRSVQTKNSAYFVEWIPDNVLKAVCSVPPKDLKMSATFI 368
Query: 246 GNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ 73
GNST+IQE+F+R+ +QF+AMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ
Sbjct: 369 GNSTSIQEIFRRLGDQFSAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ 426
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 200 bits (489), Expect = 1e-52
Identities = 87/243 (35%), Positives = 146/243 (60%), Gaps = 8/243 (3%)
Frame = -3
Query: 783 HQLVENTDESYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNAD 604
H ++N+D ++ +DNEA YDIC R L + PTY +LN L++ +S +T LRF G LN D
Sbjct: 196 HATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVVSSITASLRFAGSLNVD 255
Query: 603 LRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGR 424
L + N+VP+PR+HF + ++P+ S + + +V E+T Q F+ N M CDPR GR
Sbjct: 256 LNEFQTNLVPYPRIHFPLVTYSPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPRTGR 315
Query: 423 YLTVAAVFRGRMSMKEVDEQMMNIQNKNSSYFVEWIPNNVKTAVCDIPPR--------GL 268
Y+ ++RG + ++V + +I+++ + FV+W P K +C PP+ +
Sbjct: 316 YMATCLLYRGDVIPRDVQAAVTSIKSRRTIQFVDWCPTGFKIGICYEPPQHVPGSGIAKV 375
Query: 267 KMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSE 88
+ + N+T+I E + R+ +F M+ ++AF+HWY GEGM+E EF+EA ++ L +
Sbjct: 376 NRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERD 435
Query: 87 YQQ 79
Y++
Sbjct: 436 YEE 438
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 199 bits (485), Expect = 4e-52
Identities = 88/243 (36%), Positives = 144/243 (59%), Gaps = 8/243 (3%)
Frame = -3
Query: 783 HQLVENTDESYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNAD 604
H ++ D ++ +DNE+ YDIC R L + P+Y +LN L++ +S +T LRF G LN D
Sbjct: 192 HATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITASLRFEGSLNVD 251
Query: 603 LRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGR 424
L + N+VP+PR+HF + +AP+ S + + +V E+T Q F+ N M CDPR GR
Sbjct: 252 LAEFQTNLVPYPRIHFPLVTYAPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPRAGR 311
Query: 423 YLTVAAVFRGRMSMKEVDEQMMNIQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMS----- 259
Y+ ++RG + ++V + I+ K + FV+W P K +CD PP+ ++ S
Sbjct: 312 YMATCLLYRGDVIPRDVQAAVTTIKAKRTIQFVDWCPTGFKIGICDRPPQHIEGSEIAKV 371
Query: 258 ---ATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSE 88
+ N+T+I E + R+ +F M+ ++AF+HWY GEGM+E EF+EA ++ L +
Sbjct: 372 DRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERD 431
Query: 87 YQQ 79
Y++
Sbjct: 432 YEE 434
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 129 bits (311), Expect = 5e-31
Identities = 70/243 (28%), Positives = 129/243 (53%), Gaps = 10/243 (4%)
Frame = -3
Query: 780 QLVENTDESYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADL 601
+L N D +DN AL I L PT+ N LVS MS TT LR+PG +N DL
Sbjct: 194 RLTLNADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMSASTTTLRYPGYMNNDL 253
Query: 600 RKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRAL---TVPELTQQMFDAKNMMAACDP-R 433
+ +++P PR HF + + P T++ ++ +A+ TV ++ +++ KN M + +P +
Sbjct: 254 VSIIASLIPSPRCHFLLTSYTPFTNQQVEEAKAIRKTTVLDVMRRLLLPKNQMVSVNPSK 313
Query: 432 HGRYLTVAAVFRGRMSMKEVDEQMMNIQNKNSSYFVEWIPNNVKTAVCDIPP---RGLKM 262
++++ + +G +V + ++ I+ + + F+ W P +++ A+ P ++
Sbjct: 314 KSCFISILDIIQGEADPADVHKSLLRIRERRYASFIPWGPASIQVALSKKSPYIKTNHRV 373
Query: 261 SATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMN---DLVS 91
S + N T+I LFKR +Q+ + +R AFL Y E + E + E +S+ + DL++
Sbjct: 374 SGLMLANHTSIASLFKRTLDQYDRLRKRNAFLEQYKKEAIFEDDLNEFDSSRDVVADLIN 433
Query: 90 EYQ 82
EY+
Sbjct: 434 EYE 436
>SPBP4H10.04 |ppb1||calcineurin catalytic subunit
Ppb1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 554
Score = 29.9 bits (64), Expect = 0.45
Identities = 17/66 (25%), Positives = 33/66 (50%), Gaps = 2/66 (3%)
Frame = -3
Query: 405 VFRGRMSMKEVDEQMMNIQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIG--NSTA 232
V+ + ++ + + +MNI+ N S W+PN + +P G K+S I N +
Sbjct: 343 VYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVSEMLISMLNICS 402
Query: 231 IQELFK 214
+EL++
Sbjct: 403 KEELYE 408
>SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein
L2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 363
Score = 28.3 bits (60), Expect = 1.4
Identities = 14/41 (34%), Positives = 20/41 (48%)
Frame = +3
Query: 609 HSTDQGTVDKSSRLTLSPKQGGSSRHKSGLLASAFGSRCRT 731
H T + L P+ GG H+SG +AFG+ CR+
Sbjct: 60 HQTSAESWGTGRALARIPRVGGGGTHRSG--QAAFGNMCRS 98
>SPBC557.04 |ppk29||Ark1/Prk1 family protein kinase
Ppk29|Schizosaccharomyces pombe|chr 2|||Manual
Length = 872
Score = 28.3 bits (60), Expect = 1.4
Identities = 14/36 (38%), Positives = 21/36 (58%)
Frame = -3
Query: 243 NSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDE 136
NS+ IQ L K I+ T +R ++ Y+G G+DE
Sbjct: 193 NSSEIQALEKSINTFTTYQYRAPEMINLYSGLGIDE 228
>SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein
L2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 363
Score = 28.3 bits (60), Expect = 1.4
Identities = 14/41 (34%), Positives = 20/41 (48%)
Frame = +3
Query: 609 HSTDQGTVDKSSRLTLSPKQGGSSRHKSGLLASAFGSRCRT 731
H T + L P+ GG H+SG +AFG+ CR+
Sbjct: 60 HQTSAESWGTGRALARIPRVGGGGTHRSG--QAAFGNMCRS 98
>SPBC27B12.05 |||WD repeat protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 391
Score = 26.6 bits (56), Expect = 4.2
Identities = 10/23 (43%), Positives = 15/23 (65%)
Frame = -3
Query: 678 LNHLVSATMSGVTTCLRFPGQLN 610
L HLV A + G++ C +PG+ N
Sbjct: 331 LTHLVDAPLLGMSVCPLYPGEEN 353
>SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein
Ubr1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1958
Score = 26.2 bits (55), Expect = 5.5
Identities = 18/59 (30%), Positives = 29/59 (49%)
Frame = -3
Query: 249 IGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ 73
+ N + L+K + E+F+ +F RK L WY G+ E + N+N SE + Q
Sbjct: 1703 LNNPHLLFTLYKLL-ERFSLIFLRKCALLWYCRYGVS----FETQPNLNFQNSELSRLQ 1756
>SPAC343.10 |met11|mthfr2|methylenetetrahydrofolate reductase
Met11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 641
Score = 26.2 bits (55), Expect = 5.5
Identities = 13/32 (40%), Positives = 16/32 (50%)
Frame = +3
Query: 615 TDQGTVDKSSRLTLSPKQGGSSRHKSGLLASA 710
+ Q DKSS L ++ KQ S HK L A
Sbjct: 347 SQQPVADKSSNLFVTSKQSSVSGHKDNLTEEA 378
>SPBC19C7.12c |||alpha-1,2-mannosyltransferase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 390
Score = 25.8 bits (54), Expect = 7.3
Identities = 12/39 (30%), Positives = 17/39 (43%)
Frame = -3
Query: 426 RYLTVAAVFRGRMSMKEVDEQMMNIQNKNSSYFVEWIPN 310
+Y+ +VF G + NI N S EW+PN
Sbjct: 29 QYIPTISVFEGSLIDNRDTLSYFNISNLEPSERSEWLPN 67
>SPCP1E11.06 |apl4||AP-1 adaptor complex gamma subunit Apl4
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 865
Score = 25.8 bits (54), Expect = 7.3
Identities = 22/68 (32%), Positives = 31/68 (45%)
Frame = -3
Query: 780 QLVENTDESYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADL 601
Q+ NTD S N LY RT+ DLN S + GV +F G + +
Sbjct: 301 QVCTNTDSSRNAGNAILYQ-AVRTIL-------DLNSDSSLRVLGVNILAKFLGNRDNNT 352
Query: 600 RKLAVNMV 577
R +A+NM+
Sbjct: 353 RYVALNML 360
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,878,169
Number of Sequences: 5004
Number of extensions: 55218
Number of successful extensions: 170
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 164
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 168
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 396433620
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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