BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= rbmte12k18
(848 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces pombe... 39 0.001
SPBP23A10.04 |apc2||anaphase-promoting complex subunit Apc2 |Sch... 30 0.48
SPAC1F5.09c |shk2|pak2|PAK-related kinase Shk2 |Schizosaccharomy... 27 2.5
SPBC365.11 |||GRIP domain protein|Schizosaccharomyces pombe|chr ... 26 7.8
SPBC14C8.05c |meu17||glucan-alpha-1,4-glucosidase|Schizosaccharo... 26 7.8
SPAC24C9.12c |||glycine hydroxymethyltransferase |Schizosaccharo... 26 7.8
>SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 560
Score = 38.7 bits (86), Expect = 0.001
Identities = 20/82 (24%), Positives = 37/82 (45%), Gaps = 1/82 (1%)
Frame = -1
Query: 779 APKQIDNPAYKGPWVHPEIDNPEYTPDSNLYKRDEICAVGLDLWQVKSGTIFDDFLITDD 600
+P I NP + G W +I NP+Y D + + VG +LW ++ F + +
Sbjct: 350 SPPMIPNPEFIGEWYPRKIPNPDYFDDDHPSHFGPLYGVGFELWTMQPNIRFSNIYVGHS 409
Query: 599 PAAAKERG-EVIKKRQEGEKKM 537
A+ G E + + E+++
Sbjct: 410 IEDAERLGNETFLPKLKAEREL 431
Score = 37.1 bits (82), Expect = 0.003
Identities = 12/17 (70%), Positives = 15/17 (88%)
Frame = -3
Query: 831 GEWEPPMIDNPDYKGVW 781
GEW+PPMI NP+Y+G W
Sbjct: 333 GEWKPPMIRNPNYRGPW 349
Score = 35.1 bits (77), Expect = 0.013
Identities = 13/23 (56%), Positives = 15/23 (65%)
Frame = -1
Query: 776 PKQIDNPAYKGPWVHPEIDNPEY 708
P I NP Y+GPW P I NPE+
Sbjct: 337 PPMIRNPNYRGPWSPPMIPNPEF 359
Score = 31.5 bits (68), Expect = 0.16
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = -3
Query: 831 GEWEPPMIDNPDYKGVW 781
G W PPMI NP++ G W
Sbjct: 347 GPWSPPMIPNPEFIGEW 363
Score = 26.2 bits (55), Expect = 5.9
Identities = 10/16 (62%), Positives = 10/16 (62%)
Frame = -3
Query: 840 EMDGEWEPPMIDNPDY 793
E GEW P I NPDY
Sbjct: 358 EFIGEWYPRKIPNPDY 373
>SPBP23A10.04 |apc2||anaphase-promoting complex subunit Apc2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 681
Score = 29.9 bits (64), Expect = 0.48
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = +3
Query: 339 LHARTLRTPRYLMSWHSLINHNSLQLI 419
LH +L T RY MSW + H +L+ I
Sbjct: 161 LHVGSLLTTRYAMSWEQSVYHEALEWI 187
>SPAC1F5.09c |shk2|pak2|PAK-related kinase Shk2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 589
Score = 27.5 bits (58), Expect = 2.5
Identities = 12/40 (30%), Positives = 22/40 (55%)
Frame = +3
Query: 126 QNIK*LHTSILSVIPIYTDISTKQRTHIYLHTTCPYESVK 245
+N+K S L++ I+ + + TH +L+ CP E +K
Sbjct: 537 KNLKSFLNSCLTIDTIFRATAAELLTHSFLNQACPTEDLK 576
>SPBC365.11 |||GRIP domain protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 266
Score = 25.8 bits (54), Expect = 7.8
Identities = 14/32 (43%), Positives = 19/32 (59%), Gaps = 1/32 (3%)
Frame = -1
Query: 641 KSGTIF-DDFLITDDPAAAKERGEVIKKRQEG 549
KS T+F D+ IT PA+ K+R KK+ G
Sbjct: 20 KSETVFLDEAAITKPPASKKKRKNRKKKKNNG 51
>SPBC14C8.05c
|meu17||glucan-alpha-1,4-glucosidase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 450
Score = 25.8 bits (54), Expect = 7.8
Identities = 10/24 (41%), Positives = 14/24 (58%), Gaps = 1/24 (4%)
Frame = -1
Query: 782 GAPK-QIDNPAYKGPWVHPEIDNP 714
G PK +D +Y G W P+ D+P
Sbjct: 132 GEPKFNVDGTSYDGDWGRPQNDSP 155
>SPAC24C9.12c |||glycine hydroxymethyltransferase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 467
Score = 25.8 bits (54), Expect = 7.8
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = -2
Query: 379 DIKYLGVRSVRAWRILFLKPLVAQSSTIP 293
D+K GV RA R+L L +V +T+P
Sbjct: 347 DVKSKGVDGARAERVLELINIVTNKNTVP 375
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,125,724
Number of Sequences: 5004
Number of extensions: 64108
Number of successful extensions: 191
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 178
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 191
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 420459900
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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