BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= rbmte12j22
(671 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizo... 34 0.021
SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyc... 30 0.26
SPAC2C4.07c |||ribonuclease II |Schizosaccharomyces pombe|chr 1|... 29 0.46
SPCC63.14 |||conserved fungal protein|Schizosaccharomyces pombe|... 27 2.5
SPAC10F6.02c |prp22||ATP-dependent RNA helicase Prp22|Schizosacc... 27 2.5
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 26 4.3
SPBP19A11.06 |lid2|SPBP4H10.01|Lid2 complex subunit Lid2 |Schizo... 26 4.3
SPCC63.02c |aah3||alpha-amylase homolog Aah3|Schizosaccharomyces... 25 9.9
SPAC140.04 |||conserved fungal protein|Schizosaccharomyces pombe... 25 9.9
SPAC19E9.02 |fin1||serine/threonine protein kinase Fin1|Schizosa... 25 9.9
>SPAC17C9.03 |tif471||translation initiation factor eIF4G
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1403
Score = 33.9 bits (74), Expect = 0.021
Identities = 25/95 (26%), Positives = 47/95 (49%), Gaps = 2/95 (2%)
Frame = -2
Query: 481 RGLEKKLRDMDAEERRKIHEKEASIKTAQEKAKLKA--MAKEQXXXXXXXXXXXXKTGTA 308
R E+K R ++AEE K +E + + A+EKAK +A AK + A
Sbjct: 547 RKAEEKAR-LEAEENAKREAEEQAKREAEEKAKREAEEKAKREAEEKAKREAEENAKREA 605
Query: 307 ESKDAIKSDKKPEMQAGSQQVKKVEAKPQQQPAKK 203
E K ++++K + +A + ++ E K +++ +K
Sbjct: 606 EEKAKREAEEKAKREAEEKAKREAEEKAKREAEEK 640
Score = 31.1 bits (67), Expect = 0.15
Identities = 23/99 (23%), Positives = 48/99 (48%)
Frame = -2
Query: 481 RGLEKKLRDMDAEERRKIHEKEASIKTAQEKAKLKAMAKEQXXXXXXXXXXXXKTGTAES 302
R E+K + +AEE+ K +E + + A+EKAK +A K + + E+
Sbjct: 611 REAEEKAK-REAEEKAKREAEEKAKREAEEKAKREAEEKAKREAEEKAKREAEENAKREA 669
Query: 301 KDAIKSDKKPEMQAGSQQVKKVEAKPQQQPAKKVDTNVK 185
++ K+ ++ E A + +KV+ + ++ +K + K
Sbjct: 670 EE--KAKREAEENAKREAEEKVKRETEENAKRKAEEEGK 706
>SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1085
Score = 30.3 bits (65), Expect = 0.26
Identities = 16/44 (36%), Positives = 28/44 (63%), Gaps = 3/44 (6%)
Frame = -2
Query: 481 RGLEKKLRDMDA---EERRKIHEKEASIKTAQEKAKLKAMAKEQ 359
R EKK ++++ EE++K E+E +K Q++A + MA+EQ
Sbjct: 648 REQEKKQQELERQKREEKQKQKEREKKLKKQQQEADREKMAREQ 691
>SPAC2C4.07c |||ribonuclease II |Schizosaccharomyces pombe|chr
1|||Manual
Length = 927
Score = 29.5 bits (63), Expect = 0.46
Identities = 19/70 (27%), Positives = 36/70 (51%)
Frame = -2
Query: 568 APPMTLNFFHGKVGQNLEGIVSKTVDSFIRGLEKKLRDMDAEERRKIHEKEASIKTAQEK 389
A P T + H N+ G+ S ++ I+ + ++ D + + H+K + +T EK
Sbjct: 36 AYPSTTHDPHQNDDSNIPGLGSGLLER-IKDIVQRPTDTQLKGQDSNHKKASLTETKTEK 94
Query: 388 AKLKAMAKEQ 359
AK+K AK++
Sbjct: 95 AKVKPKAKKK 104
>SPCC63.14 |||conserved fungal protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1184
Score = 27.1 bits (57), Expect = 2.5
Identities = 12/48 (25%), Positives = 30/48 (62%)
Frame = -2
Query: 526 QNLEGIVSKTVDSFIRGLEKKLRDMDAEERRKIHEKEASIKTAQEKAK 383
+N++ +VS+ + + E+K + +A+ +K+ ++E +K +EKA+
Sbjct: 696 RNVDPMVSELSERAAQERERKEQAKEAKRLKKLAKEEKRLKKKEEKAR 743
>SPAC10F6.02c |prp22||ATP-dependent RNA helicase
Prp22|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1168
Score = 27.1 bits (57), Expect = 2.5
Identities = 11/22 (50%), Positives = 16/22 (72%)
Frame = -2
Query: 448 AEERRKIHEKEASIKTAQEKAK 383
A +RR+I +KEA +K+ QE K
Sbjct: 425 ANDRREIRQKEAKLKSEQEMEK 446
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 26.2 bits (55), Expect = 4.3
Identities = 21/79 (26%), Positives = 34/79 (43%)
Frame = -2
Query: 418 EASIKTAQEKAKLKAMAKEQXXXXXXXXXXXXKTGTAESKDAIKSDKKPEMQAGSQQVKK 239
E S+ +KA KA AKE+ +SK+A+ +D K + S +K
Sbjct: 67 EKSVNYLLQKASSKAGAKEKQNTDSQKEKKQN-----KSKEAL-ADAKDPLDESSNGIKN 120
Query: 238 VEAKPQQQPAKKVDTNVKM 182
+ +PA + + VKM
Sbjct: 121 LSLNKNDEPAFQTNGEVKM 139
>SPBP19A11.06 |lid2|SPBP4H10.01|Lid2 complex subunit Lid2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1513
Score = 26.2 bits (55), Expect = 4.3
Identities = 11/21 (52%), Positives = 14/21 (66%)
Frame = +1
Query: 499 SWKRFLPSFVPLFREKNSKSL 561
+WK P+FV L +EKNS L
Sbjct: 1336 NWKLGSPAFVTLVKEKNSSCL 1356
>SPCC63.02c |aah3||alpha-amylase homolog Aah3|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 564
Score = 25.0 bits (52), Expect = 9.9
Identities = 14/23 (60%), Positives = 15/23 (65%)
Frame = +3
Query: 33 ADRPALVLNDYRNPNTPTTTVIK 101
A+RPAL L DY N P TVIK
Sbjct: 367 ANRPALWLTDYDQSN-PYYTVIK 388
>SPAC140.04 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 295
Score = 25.0 bits (52), Expect = 9.9
Identities = 11/23 (47%), Positives = 15/23 (65%)
Frame = -2
Query: 463 LRDMDAEERRKIHEKEASIKTAQ 395
LRD EERRK E++ +IK +
Sbjct: 263 LRDKKLEERRKFLERDYAIKLGE 285
>SPAC19E9.02 |fin1||serine/threonine protein kinase
Fin1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 722
Score = 25.0 bits (52), Expect = 9.9
Identities = 11/16 (68%), Positives = 13/16 (81%)
Frame = -2
Query: 481 RGLEKKLRDMDAEERR 434
R LEK+LRDMDA +R
Sbjct: 346 RELEKQLRDMDARYQR 361
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,110,358
Number of Sequences: 5004
Number of extensions: 35681
Number of successful extensions: 106
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 93
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 96
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 307866294
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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