Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= rbmte12h22
         (748 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   338   6e-94
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   178   8e-46
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   176   2e-45
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...   122   7e-29
SPBC106.10 |pka1|tpk, git6|cAMP-dependent protein kinase catalyt...    28   1.6  
SPCP1E11.06 |apl4||AP-1 adaptor complex gamma subunit Apl4 |Schi...    26   5.0  
SPAC222.07c |hri2||eIF2 alpha kinase Hri2|Schizosaccharomyces po...    26   6.6  
SPAC2C4.07c |||ribonuclease II |Schizosaccharomyces pombe|chr 1|...    25   8.7  
SPAC4F10.09c |||ribosome biogenesis protein Noc1 |Schizosaccharo...    25   8.7  
SPAC25G10.01 ||SPAC2C4.18|RNA-binding protein|Schizosaccharomyce...    25   8.7  

>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  338 bits (830), Expect = 6e-94
 Identities = 152/227 (66%), Positives = 189/227 (83%)
 Frame = -1

Query: 748 ALYYICHRTLKLMTXTYGDLNHXVSLTMSGVTTCLRFPGQLNADLRKLAVNMIPFPRLHF 569
           AL  I   TLK+ + +Y DLNH VS  M+GVTT  RFPG+LN+DLRKLAVNM+PFPRLHF
Sbjct: 206 ALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTTSFRFPGELNSDLRKLAVNMVPFPRLHF 265

Query: 568 FMPGFAPLTSRNSQQYRALTVPELTQQMFDAKNMMAACDPHRGRYLTVATVFRGRMSMKE 389
           FM GFAPL +  S  ++A++VPELTQQMFDA NMM A DP  GRYLTVA +FRG++SMKE
Sbjct: 266 FMVGFAPLAAIGSSSFQAVSVPELTQQMFDANNMMVAADPRHGRYLTVAALFRGKVSMKE 325

Query: 388 IDEQILNVQKKNKDFFVEWIPNNVQTAVCDIPPRGMKMSATFIGNTTAIQEIFKRISEQF 209
           +DEQI +VQ KN  +FVEWIP+NV  AVC +PP+ +KMSATFIGN+T+IQEIF+R+ +QF
Sbjct: 326 VDEQIRSVQTKNSAYFVEWIPDNVLKAVCSVPPKDLKMSATFIGNSTSIQEIFRRLGDQF 385

Query: 208 AAMFSRKAFLHWYTGEGMEEGDFAEADNNVSDLLSEYQQYQDATIDQ 68
           +AMF RKAFLHWYTGEGM+E +F EA++N++DL+SEYQQYQ+A ID+
Sbjct: 386 SAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAGIDE 432


>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  178 bits (433), Expect = 8e-46
 Identities = 78/225 (34%), Positives = 131/225 (58%), Gaps = 8/225 (3%)
 Frame = -1

Query: 742 YYICHRTLKLMTXTYGDLNHXVSLTMSGVTTCLRFPGQLNADLRKLAVNMIPFPRLHFFM 563
           Y IC R L +   +Y +LN  ++  +S +T  LRF G LN DL +   N++P+PR+HF +
Sbjct: 210 YDICRRNLDIERPSYENLNRLIAQVVSSITASLRFEGSLNVDLAEFQTNLVPYPRIHFPL 269

Query: 562 PGFAPLTSRNSQQYRALTVPELTQQMFDAKNMMAACDPHRGRYLTVATVFRGRMSMKEID 383
             +AP+ S     + + +V E+T Q F+  N M  CDP  GRY+    ++RG +  +++ 
Sbjct: 270 VTYAPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPRAGRYMATCLLYRGDVIPRDVQ 329

Query: 382 EQILNVQKKNKDFFVEWIPNNVQTAVCDIPPRGMKMS--------ATFIGNTTAIQEIFK 227
             +  ++ K    FV+W P   +  +CD PP+ ++ S           + NTT+I E + 
Sbjct: 330 AAVTTIKAKRTIQFVDWCPTGFKIGICDRPPQHIEGSEIAKVDRAVCMLSNTTSIAEAWS 389

Query: 226 RISEQFAAMFSRKAFLHWYTGEGMEEGDFAEADNNVSDLLSEYQQ 92
           R+  +F  M+S++AF+HWY GEGMEEG+F+EA  +++ L  +Y++
Sbjct: 390 RLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYEE 434


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  176 bits (429), Expect = 2e-45
 Identities = 77/234 (32%), Positives = 134/234 (57%), Gaps = 8/234 (3%)
 Frame = -1

Query: 748 ALYYICHRTLKLMTXTYGDLNHXVSLTMSGVTTCLRFPGQLNADLRKLAVNMIPFPRLHF 569
           A Y IC R L +   TY +LN  ++  +S +T  LRF G LN DL +   N++P+PR+HF
Sbjct: 212 ACYDICRRNLDIERPTYENLNRLIAQVVSSITASLRFAGSLNVDLNEFQTNLVPYPRIHF 271

Query: 568 FMPGFAPLTSRNSQQYRALTVPELTQQMFDAKNMMAACDPHRGRYLTVATVFRGRMSMKE 389
            +  ++P+ S     + + +V E+T Q F+  N M  CDP  GRY+    ++RG +  ++
Sbjct: 272 PLVTYSPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPRTGRYMATCLLYRGDVIPRD 331

Query: 388 IDEQILNVQKKNKDFFVEWIPNNVQTAVCDIPPR--------GMKMSATFIGNTTAIQEI 233
           +   + +++ +    FV+W P   +  +C  PP+         +  +   + NTT+I E 
Sbjct: 332 VQAAVTSIKSRRTIQFVDWCPTGFKIGICYEPPQHVPGSGIAKVNRAVCMLSNTTSIAEA 391

Query: 232 FKRISEQFAAMFSRKAFLHWYTGEGMEEGDFAEADNNVSDLLSEYQQYQDATID 71
           + R+  +F  M+S++AF+HWY GEGMEEG+F+EA  +++ L  +Y++    ++D
Sbjct: 392 WSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYEEVGQDSMD 445


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score =  122 bits (293), Expect = 7e-29
 Identities = 66/232 (28%), Positives = 123/232 (53%), Gaps = 10/232 (4%)
 Frame = -1

Query: 748 ALYYICHRTLKLMTXTYGDLNHXVSLTMSGVTTCLRFPGQLNADLRKLAVNMIPFPRLHF 569
           AL +I    L     T+   N  VS  MS  TT LR+PG +N DL  +  ++IP PR HF
Sbjct: 209 ALAHIAADRLHTQNPTFHQQNQLVSTVMSASTTTLRYPGYMNNDLVSIIASLIPSPRCHF 268

Query: 568 FMPGFAPLTSRNSQQYRAL---TVPELTQQMFDAKNMMAACDP-HRGRYLTVATVFRGRM 401
            +  + P T++  ++ +A+   TV ++ +++   KN M + +P  +  ++++  + +G  
Sbjct: 269 LLTSYTPFTNQQVEEAKAIRKTTVLDVMRRLLLPKNQMVSVNPSKKSCFISILDIIQGEA 328

Query: 400 SMKEIDEQILNVQKKNKDFFVEWIPNNVQTAVCDIPP---RGMKMSATFIGNTTAIQEIF 230
              ++ + +L ++++    F+ W P ++Q A+    P      ++S   + N T+I  +F
Sbjct: 329 DPADVHKSLLRIRERRYASFIPWGPASIQVALSKKSPYIKTNHRVSGLMLANHTSIASLF 388

Query: 229 KRISEQFAAMFSRKAFLHWYTGEGMEEGDFAEADNN---VSDLLSEYQQYQD 83
           KR  +Q+  +  R AFL  Y  E + E D  E D++   V+DL++EY+  +D
Sbjct: 389 KRTLDQYDRLRKRNAFLEQYKKEAIFEDDLNEFDSSRDVVADLINEYEACED 440


>SPBC106.10 |pka1|tpk, git6|cAMP-dependent protein kinase catalytic
           subunit Pka1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 512

 Score = 27.9 bits (59), Expect = 1.6
 Identities = 22/68 (32%), Positives = 32/68 (47%)
 Frame = -3

Query: 623 RGSTKVSGQYDTLSEAALLYARLCTADVKK*SAVPRSHGTRINTTNVRRKEYDGGL*SSP 444
           +GST V    DTLS +A L+  +    V + S   R HGT  N+ N +   +D     + 
Sbjct: 9   KGSTNVGSSTDTLSTSASLHPSMNAGSVNEYSEQQR-HGT--NSFNGKPSVHDSVGSDAS 65

Query: 443 RSLSHCSH 420
            S  H +H
Sbjct: 66  VSNGHNNH 73


>SPCP1E11.06 |apl4||AP-1 adaptor complex gamma subunit Apl4
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 865

 Score = 26.2 bits (55), Expect = 5.0
 Identities = 13/35 (37%), Positives = 20/35 (57%)
 Frame = -1

Query: 694 DLNHXVSLTMSGVTTCLRFPGQLNADLRKLAVNMI 590
           DLN   SL + GV    +F G  + + R +A+NM+
Sbjct: 326 DLNSDSSLRVLGVNILAKFLGNRDNNTRYVALNML 360


>SPAC222.07c |hri2||eIF2 alpha kinase Hri2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 639

 Score = 25.8 bits (54), Expect = 6.6
 Identities = 10/22 (45%), Positives = 13/22 (59%)
 Frame = +2

Query: 389 FLH*HSSPEDGGYSEIATAVRI 454
           F + H S EDG YSE   A+ +
Sbjct: 3   FFNAHKSAEDGNYSETTNAISL 24


>SPAC2C4.07c |||ribonuclease II |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 927

 Score = 25.4 bits (53), Expect = 8.7
 Identities = 12/34 (35%), Positives = 18/34 (52%)
 Frame = +3

Query: 531 LFLDVSGAKPGIKKCSLGKGIILTANFRRSALSC 632
           L+ D +  K G+K  +L KG +      RSA +C
Sbjct: 178 LYYDSATVKKGLKSGTLFKGTLRILENHRSAFAC 211


>SPAC4F10.09c |||ribosome biogenesis protein Noc1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 860

 Score = 25.4 bits (53), Expect = 8.7
 Identities = 13/33 (39%), Positives = 19/33 (57%), Gaps = 2/33 (6%)
 Frame = -1

Query: 556 FAPLTSRNSQQYRALTVPE--LTQQMFDAKNMM 464
           FAP TSR S  Y  +T+ +  LT +  D  N++
Sbjct: 276 FAPSTSRTSCYYTLITLNQTVLTHKQVDVANLL 308


>SPAC25G10.01 ||SPAC2C4.18|RNA-binding protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 297

 Score = 25.4 bits (53), Expect = 8.7
 Identities = 19/72 (26%), Positives = 34/72 (47%), Gaps = 1/72 (1%)
 Frame = -1

Query: 403 MSMKEIDEQILNVQKKNKDFFVEWIPNNVQTAVCDIPPRGMKMSATFIGNTTAIQEIFKR 224
           M++ ++D     +Q  N+D   E  PNN  TA+    P+     +  +GN   +  I  R
Sbjct: 55  MNVHQLDTAGEPLQSMNED---EVDPNNESTALDKKEPQSAPEGSENLGNDLFVSGIASR 111

Query: 223 ISE-QFAAMFSR 191
           + E +   +FS+
Sbjct: 112 MQEDELQQIFSK 123


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,892,457
Number of Sequences: 5004
Number of extensions: 59069
Number of successful extensions: 180
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 172
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 177
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 355273338
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)