Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= rbmte12f20
(712 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 364 e-102
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 174 1e-44
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 170 1e-43
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 118 1e-27
SPBP4H10.04 |ppb1||calcineurin catalytic subunit Ppb1|Schizosacc... 30 0.38
SPBC557.04 |ppk29||Ark1/Prk1 family protein kinase Ppk29|Schizos... 28 1.1
SPBC27B12.05 |||WD repeat protein|Schizosaccharomyces pombe|chr ... 27 3.5
SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein Ubr1|Sc... 26 4.6
SPBC19C7.12c |||alpha-1,2-mannosyltransferase|Schizosaccharomyce... 26 6.1
SPAC343.10 |met11|mthfr2|methylenetetrahydrofolate reductase Met... 25 8.1
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 364 bits (896), Expect = e-102
Identities = 163/211 (77%), Positives = 191/211 (90%)
Frame = -3
Query: 710 KLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTS 531
K+ +P+Y DLNHLVSA M+GVTT RFPG+LN+DLRKLAVNMVPFPRLHFF+ GFAPL +
Sbjct: 216 KIKSPSYDDLNHLVSAVMAGVTTSFRFPGELNSDLRKLAVNMVPFPRLHFFMVGFAPLAA 275
Query: 530 RGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMMNIQN 351
GS ++A++VPELTQQMFDA NMM A DPRHGRYLTVAA+FRG++SMKEVDEQ+ ++Q
Sbjct: 276 IGSSSFQAVSVPELTQQMFDANNMMVAADPRHGRYLTVAALFRGKVSMKEVDEQIRSVQT 335
Query: 350 KNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFL 171
KNS+YFVEWIP+NV AVC +PP+ LKMSATFIGNST+IQE+F+R+ +QF+AMFRRKAFL
Sbjct: 336 KNSAYFVEWIPDNVLKAVCSVPPKDLKMSATFIGNSTSIQEIFRRLGDQFSAMFRRKAFL 395
Query: 170 HWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ 78
HWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ
Sbjct: 396 HWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ 426
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 174 bits (423), Expect = 1e-44
Identities = 77/213 (36%), Positives = 126/213 (59%), Gaps = 8/213 (3%)
Frame = -3
Query: 698 PTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQ 519
P+Y +LN L++ +S +T LRF G LN DL + N+VP+PR+HF + +AP+ S
Sbjct: 222 PSYENLNRLIAQVVSSITASLRFEGSLNVDLAEFQTNLVPYPRIHFPLVTYAPIVSAAKA 281
Query: 518 QYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMMNIQNKNSS 339
+ + +V E+T Q F+ N M CDPR GRY+ ++RG + ++V + I+ K +
Sbjct: 282 FHESNSVQEITNQCFEPYNQMVKCDPRAGRYMATCLLYRGDVIPRDVQAAVTTIKAKRTI 341
Query: 338 YFVEWIPNNVKTAVCDIPPRGLKMS--------ATFIGNSTAIQELFKRISEQFTAMFRR 183
FV+W P K +CD PP+ ++ S + N+T+I E + R+ +F M+ +
Sbjct: 342 QFVDWCPTGFKIGICDRPPQHIEGSEIAKVDRAVCMLSNTTSIAEAWSRLDHKFDLMYSK 401
Query: 182 KAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQ 84
+AF+HWY GEGM+E EF+EA ++ L +Y++
Sbjct: 402 RAFVHWYVGEGMEEGEFSEAREDLAALERDYEE 434
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 170 bits (414), Expect = 1e-43
Identities = 74/213 (34%), Positives = 126/213 (59%), Gaps = 8/213 (3%)
Frame = -3
Query: 698 PTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQ 519
PTY +LN L++ +S +T LRF G LN DL + N+VP+PR+HF + ++P+ S
Sbjct: 226 PTYENLNRLIAQVVSSITASLRFAGSLNVDLNEFQTNLVPYPRIHFPLVTYSPIVSAAKA 285
Query: 518 QYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMMNIQNKNSS 339
+ + +V E+T Q F+ N M CDPR GRY+ ++RG + ++V + +I+++ +
Sbjct: 286 FHESNSVQEITNQCFEPYNQMVKCDPRTGRYMATCLLYRGDVIPRDVQAAVTSIKSRRTI 345
Query: 338 YFVEWIPNNVKTAVCDIPPR--------GLKMSATFIGNSTAIQELFKRISEQFTAMFRR 183
FV+W P K +C PP+ + + + N+T+I E + R+ +F M+ +
Sbjct: 346 QFVDWCPTGFKIGICYEPPQHVPGSGIAKVNRAVCMLSNTTSIAEAWSRLDHKFDLMYSK 405
Query: 182 KAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQ 84
+AF+HWY GEGM+E EF+EA ++ L +Y++
Sbjct: 406 RAFVHWYVGEGMEEGEFSEAREDLAALERDYEE 438
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 118 bits (283), Expect = 1e-27
Identities = 61/214 (28%), Positives = 118/214 (55%), Gaps = 10/214 (4%)
Frame = -3
Query: 698 PTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQ 519
PT+ N LVS MS TT LR+PG +N DL + +++P PR HF + + P T++ +
Sbjct: 223 PTFHQQNQLVSTVMSASTTTLRYPGYMNNDLVSIIASLIPSPRCHFLLTSYTPFTNQQVE 282
Query: 518 QYRAL---TVPELTQQMFDAKNMMAACDP-RHGRYLTVAAVFRGRMSMKEVDEQMMNIQN 351
+ +A+ TV ++ +++ KN M + +P + ++++ + +G +V + ++ I+
Sbjct: 283 EAKAIRKTTVLDVMRRLLLPKNQMVSVNPSKKSCFISILDIIQGEADPADVHKSLLRIRE 342
Query: 350 KNSSYFVEWIPNNVKTAVCDIPP---RGLKMSATFIGNSTAIQELFKRISEQFTAMFRRK 180
+ + F+ W P +++ A+ P ++S + N T+I LFKR +Q+ + +R
Sbjct: 343 RRYASFIPWGPASIQVALSKKSPYIKTNHRVSGLMLANHTSIASLFKRTLDQYDRLRKRN 402
Query: 179 AFLHWYTGEGMDEMEFTEAESNMN---DLVSEYQ 87
AFL Y E + E + E +S+ + DL++EY+
Sbjct: 403 AFLEQYKKEAIFEDDLNEFDSSRDVVADLINEYE 436
>SPBP4H10.04 |ppb1||calcineurin catalytic subunit
Ppb1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 554
Score = 29.9 bits (64), Expect = 0.38
Identities = 17/66 (25%), Positives = 33/66 (50%), Gaps = 2/66 (3%)
Frame = -3
Query: 410 VFRGRMSMKEVDEQMMNIQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIG--NSTA 237
V+ + ++ + + +MNI+ N S W+PN + +P G K+S I N +
Sbjct: 343 VYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVSEMLISMLNICS 402
Query: 236 IQELFK 219
+EL++
Sbjct: 403 KEELYE 408
>SPBC557.04 |ppk29||Ark1/Prk1 family protein kinase
Ppk29|Schizosaccharomyces pombe|chr 2|||Manual
Length = 872
Score = 28.3 bits (60), Expect = 1.1
Identities = 14/36 (38%), Positives = 21/36 (58%)
Frame = -3
Query: 248 NSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDE 141
NS+ IQ L K I+ T +R ++ Y+G G+DE
Sbjct: 193 NSSEIQALEKSINTFTTYQYRAPEMINLYSGLGIDE 228
>SPBC27B12.05 |||WD repeat protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 391
Score = 26.6 bits (56), Expect = 3.5
Identities = 10/23 (43%), Positives = 15/23 (65%)
Frame = -3
Query: 683 LNHLVSATMSGVTTCLRFPGQLN 615
L HLV A + G++ C +PG+ N
Sbjct: 331 LTHLVDAPLLGMSVCPLYPGEEN 353
>SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein
Ubr1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1958
Score = 26.2 bits (55), Expect = 4.6
Identities = 18/59 (30%), Positives = 29/59 (49%)
Frame = -3
Query: 254 IGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ 78
+ N + L+K + E+F+ +F RK L WY G+ E + N+N SE + Q
Sbjct: 1703 LNNPHLLFTLYKLL-ERFSLIFLRKCALLWYCRYGVS----FETQPNLNFQNSELSRLQ 1756
>SPBC19C7.12c |||alpha-1,2-mannosyltransferase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 390
Score = 25.8 bits (54), Expect = 6.1
Identities = 12/39 (30%), Positives = 17/39 (43%)
Frame = -3
Query: 431 RYLTVAAVFRGRMSMKEVDEQMMNIQNKNSSYFVEWIPN 315
+Y+ +VF G + NI N S EW+PN
Sbjct: 29 QYIPTISVFEGSLIDNRDTLSYFNISNLEPSERSEWLPN 67
>SPAC343.10 |met11|mthfr2|methylenetetrahydrofolate reductase
Met11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 641
Score = 25.4 bits (53), Expect = 8.1
Identities = 12/28 (42%), Positives = 15/28 (53%)
Frame = +2
Query: 620 TDQGTVDKSSRLTLSPKQGGSSRHKSGL 703
+ Q DKSS L ++ KQ S HK L
Sbjct: 347 SQQPVADKSSNLFVTSKQSSVSGHKDNL 374
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,467,697
Number of Sequences: 5004
Number of extensions: 45532
Number of successful extensions: 137
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 133
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 135
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 331187010
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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