Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= rbmte12e22
(707 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 253 2e-68
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 253 2e-68
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 176 2e-45
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 100 2e-22
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 46 4e-06
SPAC227.18 |lys3|SPAC2F7.01|saccharopine dehydrogenase [NAD+, L-... 26 4.6
SPCP31B10.05 |||tyrosyl-DNA phosphodiesterase |Schizosaccharomyc... 26 4.6
SPAC1006.08 |etd1||ethanol-hypersensitive mutant protein |Schizo... 26 6.1
SPCC550.05 |nse1||Smc5-6 complex non-SMC subunit 1|Schizosacchar... 25 8.0
SPBC1734.15 |rsc4|brd1|RSC complex subunit Rsc4|Schizosaccharomy... 25 8.0
SPAC227.16c |||GINS complex subunit Psf3|Schizosaccharomyces pom... 25 8.0
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 253 bits (619), Expect = 2e-68
Identities = 116/176 (65%), Positives = 134/176 (76%)
Frame = -2
Query: 673 KLRDSVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACL 494
+++DSVVA F WA+KEG M EENLR RFNI DV LH DAIHRGGGQIIPT RR +YA
Sbjct: 658 EIKDSVVAAFAWASKEGPMFEENLRSCRFNILDVVLHADAIHRGGGQIIPTARRVVYAST 717
Query: 493 LTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNES 314
L A P + EPV+L EIQ E A+GGIY VLN++RGHVF E Q GTP++ +KAYLPVNES
Sbjct: 718 LLASPIIQEPVFLVEIQVSENAMGGIYSVLNKKRGHVFSEEQRVGTPLYNIKAYLPVNES 777
Query: 313 FGFTADLRSNTGGQAFPQCVFDHWQVLPGDPCEPQSKPYNVVQETRKRKGLKEGLP 146
FGFT +LR T GQAFPQ VFDHW + GDP +P SKP +V E RKRKGLKE +P
Sbjct: 778 FGFTGELRQATAGQAFPQLVFDHWSPMSGDPLDPTSKPGQIVCEARKRKGLKENVP 833
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 253 bits (619), Expect = 2e-68
Identities = 116/176 (65%), Positives = 134/176 (76%)
Frame = -2
Query: 673 KLRDSVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACL 494
+++DSVVA F WA+KEG M EENLR RFNI DV LH DAIHRGGGQIIPT RR +YA
Sbjct: 658 EIKDSVVAAFAWASKEGPMFEENLRSCRFNILDVVLHADAIHRGGGQIIPTARRVVYAST 717
Query: 493 LTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNES 314
L A P + EPV+L EIQ E A+GGIY VLN++RGHVF E Q GTP++ +KAYLPVNES
Sbjct: 718 LLASPIIQEPVFLVEIQVSENAMGGIYSVLNKKRGHVFSEEQRVGTPLYNIKAYLPVNES 777
Query: 313 FGFTADLRSNTGGQAFPQCVFDHWQVLPGDPCEPQSKPYNVVQETRKRKGLKEGLP 146
FGFT +LR T GQAFPQ VFDHW + GDP +P SKP +V E RKRKGLKE +P
Sbjct: 778 FGFTGELRQATAGQAFPQLVFDHWSPMSGDPLDPTSKPGQIVCEARKRKGLKENVP 833
>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 983
Score = 176 bits (429), Expect = 2e-45
Identities = 78/157 (49%), Positives = 107/157 (68%)
Frame = -2
Query: 670 LRDSVVAGFQWAAKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLL 491
+++ + GFQW +EG + +E +R V F + DV L + I+RGGGQIIPT RR Y+ L
Sbjct: 775 VKEYIKQGFQWGTREGPLCDETIRNVNFRLMDVVLAPEQIYRGGGQIIPTARRVCYSSFL 834
Query: 490 TAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESF 311
TA PRLMEPVY+ E+ P ++ IY +L RRRGHV ++ G+P+++V+A +PV +S
Sbjct: 835 TASPRLMEPVYMVEVHAPADSLPIIYDLLTRRRGHVLQDIPRPGSPLYLVRALIPVIDSC 894
Query: 310 GFTADLRSNTGGQAFPQCVFDHWQVLPGDPCEPQSKP 200
GF DLR +T GQA Q VFDHWQV+PGDP + KP
Sbjct: 895 GFETDLRVHTQGQAMCQMVFDHWQVVPGDPLDKSIKP 931
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Manual
Length = 1000
Score = 100 bits (239), Expect = 2e-22
Identities = 54/159 (33%), Positives = 86/159 (54%), Gaps = 9/159 (5%)
Frame = -2
Query: 670 LRDSVVAGFQWAAKEGVMAEENLRGV-----RFNIYDVTLHTDAIHRGG----GQIIPTT 518
L + VV FQ +G + E ++G+ +F+I D + + + GQ+I
Sbjct: 788 LSEYVVTAFQLITHQGPLCAEPVQGICVSIDQFDISDDSEDSKLLTINNPQIPGQVISVV 847
Query: 517 RRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVK 338
+ + L PRLM +Y C++Q +G +YGV+++RRG V +E GTP FIVK
Sbjct: 848 KESIRHGFLGWSPRLMLAMYSCDVQATSEVLGRVYGVVSKRRGRVIDEEMKEGTPFFIVK 907
Query: 337 AYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDP 221
A +PV ESFGF ++ T G A+PQ +F +++L +P
Sbjct: 908 ALIPVVESFGFAVEILKRTSGAAYPQLIFHGFEMLDENP 946
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 46.4 bits (105), Expect = 4e-06
Identities = 30/125 (24%), Positives = 56/125 (44%)
Frame = -2
Query: 646 FQWAAKEGVMAEENLRGVRFNIYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAQPRLME 467
F A K+G + ++ RF + D H ++ T L A P ++E
Sbjct: 619 FYEALKKGFLIGHPIKNCRFVLEDGAYHPVDSSELAFRL--ATISAFRTAFLQANPMVLE 676
Query: 466 PVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRS 287
P+ I P GG+ G L++R+ + + F ++A +P+N F +++D+R+
Sbjct: 677 PIMNVSITAPVEHQGGVIGNLDKRKATIVDSD--TDEDEFTLQAEVPLNSMFSYSSDIRA 734
Query: 286 NTGGQ 272
T G+
Sbjct: 735 LTKGK 739
>SPAC227.18 |lys3|SPAC2F7.01|saccharopine dehydrogenase [NAD+,
L-lysine forming] |Schizosaccharomyces pombe|chr
1|||Manual
Length = 368
Score = 26.2 bits (55), Expect = 4.6
Identities = 14/40 (35%), Positives = 22/40 (55%)
Frame = +3
Query: 519 VVGMIWPPPLWMASVWSVTS*MLNLTPRKFSSAITPSLAA 638
V G+ PPPL + S+ + + + + FS A+ PSL A
Sbjct: 305 VKGVTTPPPLEVISIDHLPTLLPRESSEAFSEALIPSLLA 344
>SPCP31B10.05 |||tyrosyl-DNA phosphodiesterase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 536
Score = 26.2 bits (55), Expect = 4.6
Identities = 30/139 (21%), Positives = 54/139 (38%), Gaps = 4/139 (2%)
Frame = -3
Query: 636 PLRKELWLKRICVVLDSTSMM*HSILMPSIEV-VAKSFQQLEDACTHVC*LLS--PVLWS 466
P K+ W CV L+ H+ + P+++V + + D+ + ++ PV
Sbjct: 50 PQLKQTWQFNFCVDLNFLLENMHASVFPTVDVRITHGYDSKSDSLARLTAQMNHCPVNVK 109
Query: 465 LYIFVKFSVLK*LWXXXXXXXXXXXXTFSKSPRWQVHLCSL*RPTYLSMSRSVL-LPICV 289
LY SV +W S + +H +L P ++ MS+++ P+
Sbjct: 110 LY-----SVYVPMWGTHHSKIMVNFFK-DDSCQIVIHTANLVEPDWIGMSQAIFKTPLLY 163
Query: 288 PTPADRPSRSAYSTIGRSS 232
P D S S+ G S
Sbjct: 164 PKANDSLSTSSVPEYGNPS 182
>SPAC1006.08 |etd1||ethanol-hypersensitive mutant protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 391
Score = 25.8 bits (54), Expect = 6.1
Identities = 11/29 (37%), Positives = 17/29 (58%)
Frame = +2
Query: 521 CWNDLATTSMDGISMECYIIDVESNTTQI 607
CW++L+TTS + + II + TT I
Sbjct: 211 CWDELSTTSPESSKVSEPIIQDNTQTTHI 239
>SPCC550.05 |nse1||Smc5-6 complex non-SMC subunit
1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 232
Score = 25.4 bits (53), Expect = 8.0
Identities = 12/27 (44%), Positives = 14/27 (51%)
Frame = -2
Query: 247 HWQVLPGDPCEPQSKPYNVVQETRKRK 167
H+Q L GDP + PY VQ RK
Sbjct: 78 HFQNLSGDPVSQMATPYPPVQIELMRK 104
>SPBC1734.15 |rsc4|brd1|RSC complex subunit Rsc4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 542
Score = 25.4 bits (53), Expect = 8.0
Identities = 17/67 (25%), Positives = 29/67 (43%), Gaps = 1/67 (1%)
Frame = -2
Query: 529 IPTTRRCLYACLLTAQPRLMEPVYL-CEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTP 353
IP T RC+ + + + P+L P L + CP + + V+ H F + P
Sbjct: 386 IPNTERCILSAFICSPPQLPLPNPLRMYLPCPSLNSTEV-SVITLAPQHSFLNIVINLNP 444
Query: 352 MFIVKAY 332
+K+Y
Sbjct: 445 ALALKSY 451
>SPAC227.16c |||GINS complex subunit Psf3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 166
Score = 25.4 bits (53), Expect = 8.0
Identities = 12/42 (28%), Positives = 21/42 (50%)
Frame = +3
Query: 543 PLWMASVWSVTS*MLNLTPRKFSSAITPSLAAHWNPATTESL 668
P W+A V ++ S + P FSS + +L A+ N + +
Sbjct: 43 PFWLAEVLAINSFVSIHMPAPFSSVVRNALKANPNSVSIRDI 84
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,061,607
Number of Sequences: 5004
Number of extensions: 65695
Number of successful extensions: 163
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 156
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 162
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 329179816
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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