Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= rbmte12d20
         (611 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC6G9.06c |pcp1||pericentrin Pcp1|Schizosaccharomyces pombe|ch...    33   0.043
SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyce...    30   0.23 
SPBC11C11.03 |ndc80|ndc10, tid3|spindle pole body protein Ndc80|...    28   0.93 
SPBC119.12 |||Golgi matrix protein |Schizosaccharomyces pombe|ch...    28   0.93 
SPAC29E6.04 |nnf1|SPAC30.08|kinetochore protein Nnf1|Schizosacch...    28   1.2  
SPAC27D7.02c |||GRIP domain protein|Schizosaccharomyces pombe|ch...    28   1.2  
SPBC14C8.08c |||dubious|Schizosaccharomyces pombe|chr 2|||Manual       28   1.2  
SPBC543.03c |pku80||Ku domain protein Pku80|Schizosaccharomyces ...    27   2.1  
SPAC1783.01 |||FAD binding protein|Schizosaccharomyces pombe|chr...    26   4.9  
SPCC364.02c |bis1||stress response protein Bis1|Schizosaccharomy...    26   4.9  
SPAC26A3.09c |rga2||GTPase activating protein Rga2|Schizosacchar...    26   4.9  
SPAC29A4.06c |||human CCDC55 homolog|Schizosaccharomyces pombe|c...    26   4.9  
SPAC823.04 |||rRNA processing protein, DUF947|Schizosaccharomyce...    26   4.9  
SPCC970.10c |brl2|rfp1|ubiquitin-protein ligase E3 Brl2|Schizosa...    25   6.5  
SPBC16E9.02c |||CUE domain protein Cue5 |Schizosaccharomyces pom...    25   6.5  
SPCC162.08c |nup211||nuclear pore complex associated protein|Sch...    25   6.5  

>SPAC6G9.06c |pcp1||pericentrin Pcp1|Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1208

 Score = 32.7 bits (71), Expect = 0.043
 Identities = 21/63 (33%), Positives = 33/63 (52%)
 Frame = -3

Query: 471  SKIRETQNEXDNLHAIXYDVRLKYREIMESLKKDLMYNEKECKRLQEQIEWVSRRRAELK 292
            SKI   +   ++L     D  LK R +   L  D+    K+  +LQE+I+W+ R R+ L 
Sbjct: 921  SKIEYLEKTIEDLKLALQD-ELKNRNL---LMDDISSYNKQTTKLQEKIKWLERERSILI 976

Query: 291  DEV 283
            DE+
Sbjct: 977  DEL 979



 Score = 27.1 bits (57), Expect = 2.1
 Identities = 13/33 (39%), Positives = 17/33 (51%)
 Frame = -3

Query: 546 DSLDEHLDVLTRKLADKEGRLRLSKSKIRETQN 448
           D L E LDV    L +KE  +   K +I E +N
Sbjct: 295 DELSEELDVAQDLLTEKEDEIATLKRQIEEKEN 327


>SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1957

 Score = 30.3 bits (65), Expect = 0.23
 Identities = 22/88 (25%), Positives = 36/88 (40%)
 Frame = -3

Query: 555 EQKDSLDEHLDVLTRKLADKEGRLRLSKSKIRETQNEXDNLHAIXYDVRLKYREIMESLK 376
           E  DSL++++  L   L   E  LR SK + +  +   DNL      +  +  ++  SL 
Sbjct: 682 EDVDSLEKNIQTLKEDLRKSEEALRFSKLEAKNLREVIDNLKGKHETLEAQRNDLHSSLS 741

Query: 375 KDLMYNEKECKRLQEQIEWVSRRRAELK 292
                N      L +  E V R  A ++
Sbjct: 742 DAKNTNAILSSELTKSSEDVKRLTANVE 769



 Score = 29.9 bits (64), Expect = 0.30
 Identities = 21/87 (24%), Positives = 40/87 (45%)
 Frame = -3

Query: 543  SLDEHLDVLTRKLADKEGRLRLSKSKIRETQNEXDNLHAIXYDVRLKYREIMESLKKDLM 364
            S  E L +   +      R+   ++++++  N  D+L      +R    + + SL+K+  
Sbjct: 1605 STTEELQLAENERLSLTTRMLDLQNQVKDLSNIKDSLSEDLRTLR-SLEDSVASLQKECK 1663

Query: 363  YNEKECKRLQEQIEWVSRRRAELKDEV 283
                  + LQ+ +  V  R AEL+DEV
Sbjct: 1664 IKSNTVESLQDVLTSVQARNAELEDEV 1690


>SPBC11C11.03 |ndc80|ndc10, tid3|spindle pole body protein
           Ndc80|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 624

 Score = 28.3 bits (60), Expect = 0.93
 Identities = 17/53 (32%), Positives = 33/53 (62%), Gaps = 4/53 (7%)
 Frame = -3

Query: 426 IXYDVRLKYREIMESLKKD--LMYNEKECK--RLQEQIEWVSRRRAELKDEVR 280
           I  D++ K R  +  +++   L ++E++ K  +LQE ++ V+   AEL+DE+R
Sbjct: 454 INLDLKNKVRPFINEVRRSITLEFHEEQNKSLKLQEHVDTVNDLIAELQDELR 506


>SPBC119.12 |||Golgi matrix protein |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 401

 Score = 28.3 bits (60), Expect = 0.93
 Identities = 17/73 (23%), Positives = 34/73 (46%), Gaps = 2/73 (2%)
 Frame = -3

Query: 537 DEHLDVLT--RKLADKEGRLRLSKSKIRETQNEXDNLHAIXYDVRLKYREIMESLKKDLM 364
           DEHL+     +KLA+KE   +  K+     + +  NL      ++    E ++   ++L 
Sbjct: 46  DEHLECSESFKKLAEKEEAYQTLKNSYNSLKQQHSNLLGKVSGIKSTLGERLKKDSQELA 105

Query: 363 YNEKECKRLQEQI 325
            N K  + L++ +
Sbjct: 106 QNRKRIQELEKSL 118


>SPAC29E6.04 |nnf1|SPAC30.08|kinetochore protein
           Nnf1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 205

 Score = 27.9 bits (59), Expect = 1.2
 Identities = 22/79 (27%), Positives = 43/79 (54%), Gaps = 1/79 (1%)
 Frame = -3

Query: 558 LEQKDSLDEHLDVLTRKLADKEGRLRLSKSKIRETQNEXDN-LHAIXYDVRLKYREIMES 382
           ++++D L++ LD+L   + D E R    +S++  +  +    L A  Y  +   RE+++ 
Sbjct: 64  IKERD-LNKKLDMLDECIHDAEFRKLHGESEVDISNKQPQEILKAHLYSHK---RELLDK 119

Query: 381 LKKDLMYNEKECKRLQEQI 325
           L +DL+  +KE + L  QI
Sbjct: 120 LNQDLLDIDKENEGLSTQI 138


>SPAC27D7.02c |||GRIP domain protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 750

 Score = 27.9 bits (59), Expect = 1.2
 Identities = 22/100 (22%), Positives = 44/100 (44%), Gaps = 1/100 (1%)
 Frame = -3

Query: 558 LEQKDSLDEHLDVLTRKLADKEGRLRLSKSKIRETQNEXDNLHAIXY-DVRLKYREIMES 382
           L+ K+     L  L R+      +LRL   ++   + +        Y ++   Y   ++S
Sbjct: 511 LQNKEEELTELRKLLREQTQDSQKLRLLVEQLELERQDLKQAGENHYSNLSSDYETQIKS 570

Query: 381 LKKDLMYNEKECKRLQEQIEWVSRRRAELKDEVRLGQRLY 262
           L+  L  ++ EC   QE+I  ++ +  ELK ++    + Y
Sbjct: 571 LESSLTNSQAECVSFQEKINELNSQIDELKLKLNEANKKY 610


>SPBC14C8.08c |||dubious|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 182

 Score = 27.9 bits (59), Expect = 1.2
 Identities = 16/47 (34%), Positives = 25/47 (53%), Gaps = 2/47 (4%)
 Frame = -3

Query: 186 EKPHVQRSVTELRHRKEPHLPRPNPAASLVIKTSPPQT--SEAIVTH 52
           +K  V +   ++ + KEP++  P+  AS  I    P+T  SEA V H
Sbjct: 122 KKKEVNKLEEKMTNAKEPNVNVPSMKASSAISVETPETIESEASVDH 168


>SPBC543.03c |pku80||Ku domain protein Pku80|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 695

 Score = 27.1 bits (57), Expect = 2.1
 Identities = 12/28 (42%), Positives = 17/28 (60%)
 Frame = -3

Query: 435 LHAIXYDVRLKYREIMESLKKDLMYNEK 352
           LH+I  D   ++ E +  LKKD+M N K
Sbjct: 636 LHSILEDEVERFNEYLTRLKKDVMQNNK 663


>SPAC1783.01 |||FAD binding protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 583

 Score = 25.8 bits (54), Expect = 4.9
 Identities = 23/82 (28%), Positives = 39/82 (47%), Gaps = 3/82 (3%)
 Frame = -3

Query: 567 GFRLEQKDSLDEHLDVLTRKLADKEGRLRLSKSKIRETQNEXDNLHAIXYDVRLKYREI- 391
           G R   +D++  H DV+   L+ K GR+ L  S      +    +     DV +KY ++ 
Sbjct: 504 GRREYVQDAVRRHGDVIWDVLSHKNGRIYLCGS----GNSFVSEIEKALMDVAMKYGKLS 559

Query: 390 MESLKKDLMYNEK--ECKRLQE 331
            E  +K+L   +K   CK ++E
Sbjct: 560 KEEAQKELKNWQKPMNCKLIKE 581


>SPCC364.02c |bis1||stress response protein Bis1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 384

 Score = 25.8 bits (54), Expect = 4.9
 Identities = 8/19 (42%), Positives = 11/19 (57%)
 Frame = -1

Query: 557 WSRRTASMSTWMYSPENLL 501
           W  +  S+ TW Y P+N L
Sbjct: 178 WKEKDKSIKTWNYQPKNAL 196


>SPAC26A3.09c |rga2||GTPase activating protein
           Rga2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1275

 Score = 25.8 bits (54), Expect = 4.9
 Identities = 18/41 (43%), Positives = 22/41 (53%), Gaps = 6/41 (14%)
 Frame = -2

Query: 217 ARSRPRLQDNREATRPAL------SHRAASQKGAASTATEP 113
           ARS  RL D    TRP L      SHR++ + GA+S  T P
Sbjct: 196 ARSLDRLSD---MTRPKLLLNTKRSHRSSEEPGASSPVTSP 233


>SPAC29A4.06c |||human CCDC55 homolog|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 355

 Score = 25.8 bits (54), Expect = 4.9
 Identities = 16/71 (22%), Positives = 34/71 (47%)
 Frame = -3

Query: 561 RLEQKDSLDEHLDVLTRKLADKEGRLRLSKSKIRETQNEXDNLHAIXYDVRLKYREIMES 382
           RLE KD   ++++ L      ++  L L++ +    +NE +   A    V   Y++  E 
Sbjct: 88  RLESKDRRPKYMENLIESAKKRKRDLLLARERALLKKNELEGDDATEKFVTSSYKKHREE 147

Query: 381 LKKDLMYNEKE 349
           ++K +   ++E
Sbjct: 148 VQKAIEDRKEE 158


>SPAC823.04 |||rRNA processing protein, DUF947|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 189

 Score = 25.8 bits (54), Expect = 4.9
 Identities = 27/127 (21%), Positives = 56/127 (44%), Gaps = 1/127 (0%)
 Frame = -3

Query: 480 LSKSKIRETQNEXDNLHAIXYDVRLKYREIMESLKKDLMYNEKECKRLQEQIEWVSRR-R 304
           L++ ++ E Q   D L       R +   I ++LK  L   E+  +  +E+ E V    R
Sbjct: 64  LNEYRVSEIQQLRDELKICKDQERAE--SIRQTLKSLLSKMERHLE--EERAERVMHEFR 119

Query: 303 AELKDEVRLGQRLYGXXXXXXXXXXXXLQRGRGQDYKITEKPHVQRSVTELRHRKEPHLP 124
           A+ K+ V+ G++ +              +    +  K  +K ++++       +++ HLP
Sbjct: 120 AQEKERVKEGKKPFYLKRNEQKKLIQMDKYKSMEGTKALDK-YIEKKRRRRAQKEKKHLP 178

Query: 123 RPNPAAS 103
           R  P+A+
Sbjct: 179 RARPSAN 185


>SPCC970.10c |brl2|rfp1|ubiquitin-protein ligase E3
           Brl2|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 680

 Score = 25.4 bits (53), Expect = 6.5
 Identities = 20/80 (25%), Positives = 38/80 (47%)
 Frame = -3

Query: 549 KDSLDEHLDVLTRKLADKEGRLRLSKSKIRETQNEXDNLHAIXYDVRLKYREIMESLKKD 370
           K S   + +++T+   +KE     ++ K   T    D+LHA    +R KY++  E + K 
Sbjct: 453 KSSHSHYQELITKFSVEKEK----AEQKYFLTMKSTDSLHAEVKLLRQKYQKTNEIISKM 508

Query: 369 LMYNEKECKRLQEQIEWVSR 310
           L   +    R+ E  + ++R
Sbjct: 509 LNSQDTAVHRIIEFEDQLAR 528


>SPBC16E9.02c |||CUE domain protein Cue5 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 569

 Score = 25.4 bits (53), Expect = 6.5
 Identities = 18/92 (19%), Positives = 39/92 (42%)
 Frame = -3

Query: 561 RLEQKDSLDEHLDVLTRKLADKEGRLRLSKSKIRETQNEXDNLHAIXYDVRLKYREIMES 382
           ++  +D+     DV  ++    E   +  K+  +ET+++ +   +   DV+ K  + +E 
Sbjct: 445 KITNEDTGKTETDVKAKETDSIEANDKDEKTDSKETEDKVEETESKEADVKAKETDSIEV 504

Query: 381 LKKDLMYNEKECKRLQEQIEWVSRRRAELKDE 286
             K+   + KE     EQ +         KD+
Sbjct: 505 DDKEEKTDSKETADKVEQTDSKDTNEKPAKDD 536


>SPCC162.08c |nup211||nuclear pore complex associated
           protein|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1837

 Score = 25.4 bits (53), Expect = 6.5
 Identities = 17/71 (23%), Positives = 33/71 (46%), Gaps = 7/71 (9%)
 Frame = -3

Query: 468 KIRETQNEXDNLHAIXYDVRLKYREIMES-------LKKDLMYNEKECKRLQEQIEWVSR 310
           +++E QN    L  +    +  + E +ES       LK  + + EKE   L+ Q++ ++ 
Sbjct: 311 RLKELQNSNGELEKLLEAAQSSFEEQLESHKEAEASLKSQINFLEKEVSSLESQLK-LAN 369

Query: 309 RRAELKDEVRL 277
            R    DE+ +
Sbjct: 370 ERLRHYDEIEI 380


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,165,534
Number of Sequences: 5004
Number of extensions: 38582
Number of successful extensions: 159
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 149
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 159
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 267622334
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)