Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= rbmte11e03
         (817 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce...    57   3e-09
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi...    49   7e-07
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch...    34   0.021
SPCC663.01c |ekc1|SPCC777.16c|protein phosphatase regulatory sub...    31   0.20 
SPAC869.05c |||sulfate transporter |Schizosaccharomyces pombe|ch...    27   2.4  
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma...    27   4.2  
SPAC57A10.02 |cdr2||GIN4 family protein kinase Cdr2|Schizosaccha...    27   4.2  
SPCC338.13 |cog4||Golgi transport complex subunit Cog4 |Schizosa...    26   5.6  
SPAC20H4.10 |ufd2||ubiquitin-protein ligase E4 |Schizosaccharomy...    26   7.4  
SPBC11G11.02c |end3||actin cortical patch component End3 |Schizo...    25   9.7  
SPAC9G1.02 |wis4|wak1, wik1|MAP kinase kinase kinase Wis4|Schizo...    25   9.7  
SPBC1683.04 |||glycosyl hydrolase family 3|Schizosaccharomyces p...    25   9.7  

>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 174

 Score = 57.2 bits (132), Expect = 3e-09
 Identities = 38/171 (22%), Positives = 83/171 (48%), Gaps = 1/171 (0%)
 Frame = -3

Query: 674 KKHGSMWMEIQKDTHFTFDELEKIMVIFFKIQKRDEKPAASDLISRSHFRDVLHTGLGMT 495
           +    ++ ++  ++ F+ +E+E+I   F KI         S  I R+ F  +      + 
Sbjct: 3   QSQSQIFEDLISNSSFSNEEIERIRKRFIKIDANQ-----SGSIDRNEFLSIP----SVA 53

Query: 494 DSYMMERVMVALDRGTSPYVTMATFAKAISLY-LRGDIEERIAYAFTCYDLLGEGHLRRE 318
            + +  R+   +D      V    F  ++S++ + G+ EE++ +AF  YD+  +G++   
Sbjct: 54  SNPLASRLFSVVDEDGGGDVDFQEFINSLSVFSVHGNKEEKLKFAFKIYDIDRDGYISNG 113

Query: 317 TMYQLMKKSLAKASRDDDVEEAVKELVDIMLKRMDLDHDGVINFTDFRQCV 165
            +Y ++K  +    R+D ++    ++VD  +  +D D DG I+F +F+  V
Sbjct: 114 ELYLVLKMMVGTNLREDQLQ----QIVDKTIMEVDKDRDGKISFEEFKDIV 160


>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
           Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 190

 Score = 49.2 bits (112), Expect = 7e-07
 Identities = 42/179 (23%), Positives = 79/179 (44%), Gaps = 7/179 (3%)
 Frame = -3

Query: 650 EIQKDTHFTFDELEKIMVIFFKIQKRDEKPAASDLISRSHFRDVLHTGLGMTD-SYMMER 474
           ++ + T F   EL++    FFK          S  +++S F+ +        D S   E 
Sbjct: 15  DLVRSTRFDKKELQQWYKGFFK-------DCPSGHLNKSEFQKIYKQFFPFGDPSAFAEY 67

Query: 473 VMVALDRGTSPYVTMATFAKAISLYLRGDIEERIAYAFTCYDLLGEGHLRRETMYQL--- 303
           V    D   + Y+    F  A+S+  RG++ +++ +AF  YDL   G +  + M ++   
Sbjct: 68  VFNVFDADKNGYIDFKEFICALSVTSRGELNDKLIWAFQLYDLDNNGLISYDEMLRIVDA 127

Query: 302 ---MKKSLAKASRDDDVEEAVKELVDIMLKRMDLDHDGVINFTDFRQCVLTTPSLIESL 135
              M  S+ K   D+D  E   + V+ +   MD + DG +   +F +     P+++ +L
Sbjct: 128 IYKMVGSMVKLPEDEDTPE---KRVNKIFNMMDKNKDGQLTLEEFCEGSKRDPTIVSAL 183


>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 150

 Score = 34.3 bits (75), Expect = 0.021
 Identities = 21/75 (28%), Positives = 38/75 (50%)
 Frame = -3

Query: 389 DIEERIAYAFTCYDLLGEGHLRRETMYQLMKKSLAKASRDDDVEEAVKELVDIMLKRMDL 210
           D EE +  AF  +D  G G++  E +  ++  SL +    ++V + ++E         D 
Sbjct: 82  DNEEEVREAFKVFDKDGNGYITVEELTHVLT-SLGERLSQEEVADMIREA--------DT 132

Query: 209 DHDGVINFTDFRQCV 165
           D DGVIN+ +F + +
Sbjct: 133 DGDGVINYEEFSRVI 147


>SPCC663.01c |ekc1|SPCC777.16c|protein phosphatase regulatory
           subunit Ekc1 |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 838

 Score = 31.1 bits (67), Expect = 0.20
 Identities = 28/102 (27%), Positives = 48/102 (47%), Gaps = 2/102 (1%)
 Frame = -3

Query: 734 LKMSTSLDSA--LDPMEEIRFRKKHGSMWMEIQKDTHFTFDELEKIMVIFFKIQKRDEKP 561
           +K+ + LDS   L+P++   F K +      + K T  T   ++ I     KI +  E  
Sbjct: 117 VKLWSFLDSEGPLNPLQASYFAKVNEHF---LDKKTEETVAFIQSIDNFVEKILRHAETS 173

Query: 560 AASDLISRSHFRDVLHTGLGMTDSYMMERVMVALDRGTSPYV 435
           A  DL+ +    D  +T +G+ D    + ++ +L R  SPYV
Sbjct: 174 AIMDLLLKFISMDRCNTAIGIADWLYSQGLIQSLLRLLSPYV 215


>SPAC869.05c |||sulfate transporter |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 840

 Score = 27.5 bits (58), Expect = 2.4
 Identities = 11/42 (26%), Positives = 21/42 (50%)
 Frame = -3

Query: 686 IRFRKKHGSMWMEIQKDTHFTFDELEKIMVIFFKIQKRDEKP 561
           +R  K HGS   +IQ    +  D +  +  I+  ++ ++E P
Sbjct: 536 LRIAKPHGSFLGKIQAANKYGSDNIANVRDIYVPLEMKEENP 577


>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 176

 Score = 26.6 bits (56), Expect = 4.2
 Identities = 25/101 (24%), Positives = 44/101 (43%), Gaps = 1/101 (0%)
 Frame = -3

Query: 476 RVMVALDRGTSPYVTMATFAKAIS-LYLRGDIEERIAYAFTCYDLLGEGHLRRETMYQLM 300
           +++   D+    Y+ M  F + ++   +  D  E I  AF  +D    G +    + ++ 
Sbjct: 77  KILRDFDKTGKGYLQMEDFVRVMTEKIVERDPLEEIKRAFELFDDDETGKISLRNLRRVA 136

Query: 299 KKSLAKASRDDDVEEAVKELVDIMLKRMDLDHDGVINFTDF 177
           K+     + DD   EA       M++  DLD DG IN  +F
Sbjct: 137 KE--LNENIDDQELEA-------MIEEFDLDQDGEINEQEF 168


>SPAC57A10.02 |cdr2||GIN4 family protein kinase
           Cdr2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 775

 Score = 26.6 bits (56), Expect = 4.2
 Identities = 12/28 (42%), Positives = 18/28 (64%), Gaps = 1/28 (3%)
 Frame = -2

Query: 135 RLLFTRETSCVCIFGNLV-SRLGKNVIE 55
           R LF+ E SC C++ +LV S L   ++E
Sbjct: 628 RRLFSSEPSCKCVYASLVASELEHEILE 655


>SPCC338.13 |cog4||Golgi transport complex subunit Cog4
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 738

 Score = 26.2 bits (55), Expect = 5.6
 Identities = 13/34 (38%), Positives = 19/34 (55%)
 Frame = +3

Query: 585 LEKYNHYFF*FIKSEVRIFLYFHPHRAMFFSKTY 686
           LE Y H+F   I S+ R  L   P  A+FF++ +
Sbjct: 207 LEAYWHFFGGIIASKARATLDEPPTHALFFAQAF 240


>SPAC20H4.10 |ufd2||ubiquitin-protein ligase E4 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1010

 Score = 25.8 bits (54), Expect = 7.4
 Identities = 12/36 (33%), Positives = 21/36 (58%)
 Frame = -2

Query: 111 SCVCIFGNLVSRLGKNVIE*IKHKIDRVMFTMFHKN 4
           +C+  F +++SRL +  ++    KIDRV    F +N
Sbjct: 383 ACMLNFSHVLSRLSEPFLDIGCSKIDRVQVEYFRRN 418


>SPBC11G11.02c |end3||actin cortical patch component End3
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 375

 Score = 25.4 bits (53), Expect = 9.7
 Identities = 11/34 (32%), Positives = 19/34 (55%)
 Frame = -3

Query: 215 DLDHDGVINFTDFRQCVLTTPSLIESLGYCLPER 114
           D+D DG+ +F +F   +  T  LI  +   +P+R
Sbjct: 50  DIDDDGMFDFDEFAIAMKITFDLINGVYKTVPDR 83


>SPAC9G1.02 |wis4|wak1, wik1|MAP kinase kinase kinase
            Wis4|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1401

 Score = 25.4 bits (53), Expect = 9.7
 Identities = 16/66 (24%), Positives = 29/66 (43%)
 Frame = -3

Query: 350  DLLGEGHLRRETMYQLMKKSLAKASRDDDVEEAVKELVDIMLKRMDLDHDGVINFTDFRQ 171
            DLL  G +  E + ++    L +       +  +    DI    + LDH G+I ++DF  
Sbjct: 1125 DLLAHGRIEDENVLKVYVVQLLEGLAYIHSQHILHR--DIKPANILLDHRGMIKYSDFGS 1182

Query: 170  CVLTTP 153
             +  +P
Sbjct: 1183 ALYVSP 1188


>SPBC1683.04 |||glycosyl hydrolase family 3|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 832

 Score = 25.4 bits (53), Expect = 9.7
 Identities = 15/41 (36%), Positives = 22/41 (53%), Gaps = 1/41 (2%)
 Frame = -3

Query: 215 DLDHDGVINFTDFRQCVLTTPSL-IESLGYCLPERPAVYAF 96
           DL+ DGV+ F D+    +      I+  GY +P+  AVY F
Sbjct: 423 DLE-DGVVRFYDYCNDKMKDGYFYIDIEGYLIPDEDAVYEF 462


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,371,540
Number of Sequences: 5004
Number of extensions: 70352
Number of successful extensions: 202
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 192
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 201
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 398435810
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)