Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= rbmte11d02
         (771 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC1709.06 |dus2||tRNA dihydrouridine synthase Dus2 |Schizosacc...    60   3e-10
SPCC777.15 |||tRNA dihydrouridine synthase Dus4 |Schizosaccharom...    35   0.015
SPBC646.02 |cwf11||complexed with Cdc5 protein Cwf11 |Schizosacc...    29   0.74 
SPBP8B7.14c |dpb2||DNA polymerase epsilon catalytic subunit b Dp...    29   0.97 
SPAC2G11.02 |urb2||ribosome biogenesis protein Urb2 |Schizosacch...    26   5.2  
SPAC10F6.03c |||CTP synthase |Schizosaccharomyces pombe|chr 1|||...    26   5.2  
SPBC839.16 |||C-1-tetrahydrofolate synthase|Schizosaccharomyces ...    26   5.2  
SPBC646.05c |erg9||squalene synthase Erg9|Schizosaccharomyces po...    26   6.9  
SPBC1604.17c |||conserved fungal protein|Schizosaccharomyces pom...    26   6.9  
SPBC23E6.02 |||ATP-dependent DNA helicase |Schizosaccharomyces p...    25   9.1  

>SPBC1709.06 |dus2||tRNA dihydrouridine synthase Dus2
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 479

 Score = 60.5 bits (140), Expect = 3e-10
 Identities = 32/87 (36%), Positives = 51/87 (58%)
 Frame = -1

Query: 732 VADNISIPVIANGGSKEIEKHSDVAIFKEMTGSSSVMLARAAEWNCSIFRKEGLLPMDTV 553
           V  N  + ++ NG   ++  ++D     E  G   V++ARAAE N S FR EG L    V
Sbjct: 210 VCRNKDVSILVNG---DVLSYNDGLDVIEKYGVDGVLIARAAERNVSCFRIEGPLSSFKV 266

Query: 552 IKDYLKLAVDYDNSPSNTKYCIQNILR 472
            +++LK+A++ DN+  NTKYC+  I++
Sbjct: 267 AEEFLKMALEVDNNFGNTKYCLNQIMQ 293


>SPCC777.15 |||tRNA dihydrouridine synthase Dus4
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 326

 Score = 34.7 bits (76), Expect = 0.015
 Identities = 21/62 (33%), Positives = 31/62 (50%)
 Frame = -1

Query: 771 RPQHAVHTDIIHYVADNISIPVIANGGSKEIEKHSDVAIFKEMTGSSSVMLARAAEWNCS 592
           R    V+ D I  V   + IPV+ANG  K + K  ++A + E  G   +M AR    N +
Sbjct: 184 RSSFPVNLDAIREVRPCVQIPVVANGDVKSLRKGLEIAKYTETQG---IMSARGLLENPA 240

Query: 591 IF 586
           +F
Sbjct: 241 LF 242


>SPBC646.02 |cwf11||complexed with Cdc5 protein Cwf11
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1284

 Score = 29.1 bits (62), Expect = 0.74
 Identities = 20/87 (22%), Positives = 41/87 (47%), Gaps = 3/87 (3%)
 Frame = -1

Query: 657 IFKEMTGSSSVMLARAAE-WNCSIFRKEGLLPMDTVIKDYLKLAV--DYDNSPSNTKYCI 487
           +FK+MT   + +L    +    + + KE  +  D ++  +L+L +  D+     +  +C 
Sbjct: 266 LFKKMTDDLNYVLKFPFDNTRGNEYEKEQKIRNDELVYYHLQLTLFSDFQKELGDLVFCT 325

Query: 486 QNILRDLQETPRGRQFLESQTLEQICS 406
           Q  L+  Q+      FL   +L+ +CS
Sbjct: 326 QTSLQQRQKLEEITSFLSFNSLKSLCS 352


>SPBP8B7.14c |dpb2||DNA polymerase epsilon catalytic subunit b Dpb2
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 594

 Score = 28.7 bits (61), Expect = 0.97
 Identities = 15/39 (38%), Positives = 20/39 (51%)
 Frame = +2

Query: 14  IPK*CKHTKAAPCSANFLFFSQNVDVYFFSLNVTIVRNS 130
           I + CKHT  A      +FFSQ V +Y   ++    RNS
Sbjct: 440 IQRVCKHTIFASNPCRIIFFSQEVLIYRDDISGRFQRNS 478


>SPAC2G11.02 |urb2||ribosome biogenesis protein Urb2
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1318

 Score = 26.2 bits (55), Expect = 5.2
 Identities = 13/35 (37%), Positives = 18/35 (51%)
 Frame = +2

Query: 164 LYPFFPAQA*SLLLGRFKSLKFARIKDTFICIASH 268
           LYP     A   L+  FK   F +I D F+C+ S+
Sbjct: 91  LYPII-LNAFEYLIKEFKKDVFLKISDVFVCVYSN 124


>SPAC10F6.03c |||CTP synthase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 600

 Score = 26.2 bits (55), Expect = 5.2
 Identities = 16/53 (30%), Positives = 27/53 (50%)
 Frame = -1

Query: 246 VSFIRANFNDLNLPKSRLHAWAGKNGYSLPKYNTQHIEKLFRTIVTFNEKKYT 88
           + +++  F    +  SR  A AG+N +S  K+ TQ  + LF+ +      KYT
Sbjct: 260 LEYLKIRFALDKISVSRELALAGENMWSSWKHLTQGYDHLFKKVTIVLVGKYT 312


>SPBC839.16 |||C-1-tetrahydrofolate synthase|Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 937

 Score = 26.2 bits (55), Expect = 5.2
 Identities = 22/75 (29%), Positives = 38/75 (50%), Gaps = 2/75 (2%)
 Frame = -1

Query: 681  IEKHSDVAIFKEMTGSSSVMLARAAEWNCSIFRKEGL--LPMDTVIKDYLKLAVDYDNSP 508
            IEK  ++ I KEM G+  + L+  A+     F K+G   LP+  + K    L+ D D   
Sbjct: 825  IEKKIEI-IAKEMYGADGIELSPLAKERLETFTKQGYNNLPI-CIAKTQYSLSHDPDLKG 882

Query: 507  SNTKYCIQNILRDLQ 463
            + T + +   +RD++
Sbjct: 883  APTNFTVP--IRDMR 895


>SPBC646.05c |erg9||squalene synthase Erg9|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 460

 Score = 25.8 bits (54), Expect = 6.9
 Identities = 12/24 (50%), Positives = 15/24 (62%)
 Frame = -1

Query: 573 LLPMDTVIKDYLKLAVDYDNSPSN 502
           L+  D VIK+YL L+  Y N  SN
Sbjct: 119 LVEFDVVIKEYLNLSEGYRNVISN 142


>SPBC1604.17c |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 459

 Score = 25.8 bits (54), Expect = 6.9
 Identities = 11/20 (55%), Positives = 14/20 (70%)
 Frame = +3

Query: 378 LNSILLNPKWSIFALKFDFP 437
           LNSI+LN   S+F   FD+P
Sbjct: 315 LNSIMLNGINSVFQFAFDYP 334


>SPBC23E6.02 |||ATP-dependent DNA helicase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1040

 Score = 25.4 bits (53), Expect = 9.1
 Identities = 14/57 (24%), Positives = 29/57 (50%), Gaps = 3/57 (5%)
 Frame = -1

Query: 252 MKVSFIRANFNDLNLPKSRLHAWAGK---NGYSLPKYNTQHIEKLFRTIVTFNEKKY 91
           ++++F+ +    L LP   + + AG    N  +LP ++  +  K+F T  T  + K+
Sbjct: 219 LELAFMNSKRKKLELPSLPVLSTAGPSYTNSLALPPFHHHNNYKMFNTTHTLEDDKF 275


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,197,900
Number of Sequences: 5004
Number of extensions: 66536
Number of successful extensions: 177
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 172
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 177
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 371330890
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)