Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= rbmte10o03
(759 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 368 e-103
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 174 1e-44
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 171 1e-43
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 118 9e-28
SPBP4H10.04 |ppb1||calcineurin catalytic subunit Ppb1|Schizosacc... 29 0.72
SPBC4F6.07c |||ATP-dependent RNA helicase Mak5 |Schizosaccharomy... 27 2.2
SPBC557.04 |ppk29||Ark1/Prk1 family protein kinase Ppk29|Schizos... 27 2.9
SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyce... 27 3.8
SPBC27B12.05 |||WD repeat protein|Schizosaccharomyces pombe|chr ... 27 3.8
SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein Ubr1|Sc... 26 5.1
SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal |Schizos... 26 5.1
SPBC211.03c |||guanyl-nucleotide exchange factor|Schizosaccharom... 26 5.1
SPAC140.03 |arb1||argonaute binding protein 1|Schizosaccharomyce... 26 5.1
SPBC19C7.12c |||alpha-1,2-mannosyltransferase|Schizosaccharomyce... 26 6.7
SPAC56F8.12 |||conserved fungal protein|Schizosaccharomyces pomb... 26 6.7
SPBC18H10.07 |||WW domain-binding protein 4 |Schizosaccharomyces... 26 6.7
SPCC594.06c |||SNARE Vam7 |Schizosaccharomyces pombe|chr 3|||Manual 26 6.7
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 25 8.9
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 368 bits (906), Expect = e-103
Identities = 166/214 (77%), Positives = 194/214 (90%)
Frame = -2
Query: 746 LKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLT 567
LK+ +P+Y DLNHLVSA M+GVTT RFPG+LN+DLRKLAVNMVPFPRLHFFM GFAPL
Sbjct: 215 LKIKSPSYDDLNHLVSAVMAGVTTSFRFPGELNSDLRKLAVNMVPFPRLHFFMVGFAPLA 274
Query: 566 SRGSQQYRALSVPELTQQMFDAKNMMAAWDPRHGRYLTVAAVFRGRMSMKEVDEQMLNIQ 387
+ GS ++A+SVPELTQQMFDA NMM A DPRHGRYLTVAA+FRG++SMKEVDEQ+ ++Q
Sbjct: 275 AIGSSSFQAVSVPELTQQMFDANNMMVAADPRHGRYLTVAALFRGKVSMKEVDEQIRSVQ 334
Query: 386 NKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNTTAIQELFKRISEQFTAMFRRKAF 207
KNS+YFVEWIP+NV AVC +PP+ LKMSATFIGN+T+IQE+F+R+ +QF+AMFRRKAF
Sbjct: 335 TKNSAYFVEWIPDNVLKAVCSVPPKDLKMSATFIGNSTSIQEIFRRLGDQFSAMFRRKAF 394
Query: 206 LHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDA 105
LHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+A
Sbjct: 395 LHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEA 428
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 174 bits (424), Expect = 1e-44
Identities = 79/220 (35%), Positives = 129/220 (58%), Gaps = 8/220 (3%)
Frame = -2
Query: 752 QDLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAP 573
++L + P+Y +LN L++ +S +T LRF G LN DL + N+VP+PR+HF + +AP
Sbjct: 215 RNLDIERPSYENLNRLIAQVVSSITASLRFEGSLNVDLAEFQTNLVPYPRIHFPLVTYAP 274
Query: 572 LTSRGSQQYRALSVPELTQQMFDAKNMMAAWDPRHGRYLTVAAVFRGRMSMKEVDEQMLN 393
+ S + + SV E+T Q F+ N M DPR GRY+ ++RG + ++V +
Sbjct: 275 IVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPRAGRYMATCLLYRGDVIPRDVQAAVTT 334
Query: 392 IQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMS--------ATFIGNTTAIQELFKRISEQ 237
I+ K + FV+W P K +CD PP+ ++ S + NTT+I E + R+ +
Sbjct: 335 IKAKRTIQFVDWCPTGFKIGICDRPPQHIEGSEIAKVDRAVCMLSNTTSIAEAWSRLDHK 394
Query: 236 FTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQ 117
F M+ ++AF+HWY GEGM+E EF+EA ++ L +Y++
Sbjct: 395 FDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYEE 434
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 171 bits (415), Expect = 1e-43
Identities = 76/220 (34%), Positives = 129/220 (58%), Gaps = 8/220 (3%)
Frame = -2
Query: 752 QDLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAP 573
++L + PTY +LN L++ +S +T LRF G LN DL + N+VP+PR+HF + ++P
Sbjct: 219 RNLDIERPTYENLNRLIAQVVSSITASLRFAGSLNVDLNEFQTNLVPYPRIHFPLVTYSP 278
Query: 572 LTSRGSQQYRALSVPELTQQMFDAKNMMAAWDPRHGRYLTVAAVFRGRMSMKEVDEQMLN 393
+ S + + SV E+T Q F+ N M DPR GRY+ ++RG + ++V + +
Sbjct: 279 IVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPRTGRYMATCLLYRGDVIPRDVQAAVTS 338
Query: 392 IQNKNSSYFVEWIPNNVKTAVCDIPPR--------GLKMSATFIGNTTAIQELFKRISEQ 237
I+++ + FV+W P K +C PP+ + + + NTT+I E + R+ +
Sbjct: 339 IKSRRTIQFVDWCPTGFKIGICYEPPQHVPGSGIAKVNRAVCMLSNTTSIAEAWSRLDHK 398
Query: 236 FTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQ 117
F M+ ++AF+HWY GEGM+E EF+EA ++ L +Y++
Sbjct: 399 FDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYEE 438
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 118 bits (284), Expect = 9e-28
Identities = 63/223 (28%), Positives = 121/223 (54%), Gaps = 10/223 (4%)
Frame = -2
Query: 746 LKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLT 567
L PT+ N LVS MS TT LR+PG +N DL + +++P PR HF + + P T
Sbjct: 218 LHTQNPTFHQQNQLVSTVMSASTTTLRYPGYMNNDLVSIIASLIPSPRCHFLLTSYTPFT 277
Query: 566 SRGSQQYRAL---SVPELTQQMFDAKNMMAAWDP-RHGRYLTVAAVFRGRMSMKEVDEQM 399
++ ++ +A+ +V ++ +++ KN M + +P + ++++ + +G +V + +
Sbjct: 278 NQQVEEAKAIRKTTVLDVMRRLLLPKNQMVSVNPSKKSCFISILDIIQGEADPADVHKSL 337
Query: 398 LNIQNKNSSYFVEWIPNNVKTAVCDIPP---RGLKMSATFIGNTTAIQELFKRISEQFTA 228
L I+ + + F+ W P +++ A+ P ++S + N T+I LFKR +Q+
Sbjct: 338 LRIRERRYASFIPWGPASIQVALSKKSPYIKTNHRVSGLMLANHTSIASLFKRTLDQYDR 397
Query: 227 MFRRKAFLHWYTGEGMDEMEFTEAESNMN---DLVSEYQQYQD 108
+ +R AFL Y E + E + E +S+ + DL++EY+ +D
Sbjct: 398 LRKRNAFLEQYKKEAIFEDDLNEFDSSRDVVADLINEYEACED 440
>SPBP4H10.04 |ppb1||calcineurin catalytic subunit
Ppb1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 554
Score = 29.1 bits (62), Expect = 0.72
Identities = 16/66 (24%), Positives = 33/66 (50%), Gaps = 2/66 (3%)
Frame = -2
Query: 443 VFRGRMSMKEVDEQMLNIQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIG--NTTA 270
V+ + ++ + + ++NI+ N S W+PN + +P G K+S I N +
Sbjct: 343 VYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVSEMLISMLNICS 402
Query: 269 IQELFK 252
+EL++
Sbjct: 403 KEELYE 408
>SPBC4F6.07c |||ATP-dependent RNA helicase Mak5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 648
Score = 27.5 bits (58), Expect = 2.2
Identities = 13/46 (28%), Positives = 23/46 (50%)
Frame = +3
Query: 249 SFKQLLDSCGVSDEGGRHFESTRWNVAHGRLDVIGNPFHEVAAIFV 386
+FK++ C V DE R + + + L+++GNP H F+
Sbjct: 265 NFKKI--KCLVLDEADRLLQKSHFEELSKLLEILGNPMHTQRQTFI 308
>SPBC557.04 |ppk29||Ark1/Prk1 family protein kinase
Ppk29|Schizosaccharomyces pombe|chr 2|||Manual
Length = 872
Score = 27.1 bits (57), Expect = 2.9
Identities = 13/36 (36%), Positives = 21/36 (58%)
Frame = -2
Query: 281 NTTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDE 174
N++ IQ L K I+ T +R ++ Y+G G+DE
Sbjct: 193 NSSEIQALEKSINTFTTYQYRAPEMINLYSGLGIDE 228
>SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1242
Score = 26.6 bits (56), Expect = 3.8
Identities = 15/40 (37%), Positives = 23/40 (57%)
Frame = +3
Query: 66 LYLFIKFPFIISRGVLILLVLRNQIVHVRFSLRELHFIHT 185
LY+ I F I +RG L L +N ++ + F LR ++F T
Sbjct: 1075 LYVAIPFRTIFNRGTLALDAFQNWVIGL-FMLRMIYFTVT 1113
>SPBC27B12.05 |||WD repeat protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 391
Score = 26.6 bits (56), Expect = 3.8
Identities = 10/23 (43%), Positives = 15/23 (65%)
Frame = -2
Query: 716 LNHLVSATMSGVTTCLRFPGQLN 648
L HLV A + G++ C +PG+ N
Sbjct: 331 LTHLVDAPLLGMSVCPLYPGEEN 353
>SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein
Ubr1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1958
Score = 26.2 bits (55), Expect = 5.1
Identities = 18/59 (30%), Positives = 29/59 (49%)
Frame = -2
Query: 287 IGNTTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ 111
+ N + L+K + E+F+ +F RK L WY G+ E + N+N SE + Q
Sbjct: 1703 LNNPHLLFTLYKLL-ERFSLIFLRKCALLWYCRYGVS----FETQPNLNFQNSELSRLQ 1756
>SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 647
Score = 26.2 bits (55), Expect = 5.1
Identities = 18/73 (24%), Positives = 35/73 (47%)
Frame = -3
Query: 292 PSSETPQLSKSCLKEFLNNLQPCSDEKLSCTGTRGRVWMKWSSRRLNRT*TIWFRSTSSI 113
PSS+ ++ K L F+ N+ ++ T +VW+ SS L+ T W +S++
Sbjct: 562 PSSDCSKILKCLLDGFVRNVAHLQNDGSYKTIGGKQVWLD-SSSVLHEKKTPWIMYSSAV 620
Query: 112 KTPRLMMKGNLMK 74
++ + N+ K
Sbjct: 621 ESETQIFVKNISK 633
>SPBC211.03c |||guanyl-nucleotide exchange factor|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1462
Score = 26.2 bits (55), Expect = 5.1
Identities = 13/49 (26%), Positives = 22/49 (44%), Gaps = 3/49 (6%)
Frame = +3
Query: 195 VPVQESFSSEHGCKL---FRNSFKQLLDSCGVSDEGGRHFESTRWNVAH 332
+P +S H +L F K ++ S+E HF+ TRW + +
Sbjct: 1216 LPFDKSILDNHSAELNDAFNLFLKAAVEYKVDSNESSEHFQDTRWKIIY 1264
>SPAC140.03 |arb1||argonaute binding protein 1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 399
Score = 26.2 bits (55), Expect = 5.1
Identities = 14/44 (31%), Positives = 24/44 (54%), Gaps = 2/44 (4%)
Frame = -2
Query: 464 RYLTVAAVFRGRMSMKEVDEQMLNIQNKN--SSYFVEWIPNNVK 339
RYLT V + +++K V + +LN N++ + F+ W P K
Sbjct: 297 RYLTGKVVEQEYLTVKLVSKTLLNFSNQSLCKAVFIVWDPPGSK 340
>SPBC19C7.12c |||alpha-1,2-mannosyltransferase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 390
Score = 25.8 bits (54), Expect = 6.7
Identities = 12/39 (30%), Positives = 17/39 (43%)
Frame = -2
Query: 464 RYLTVAAVFRGRMSMKEVDEQMLNIQNKNSSYFVEWIPN 348
+Y+ +VF G + NI N S EW+PN
Sbjct: 29 QYIPTISVFEGSLIDNRDTLSYFNISNLEPSERSEWLPN 67
>SPAC56F8.12 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 394
Score = 25.8 bits (54), Expect = 6.7
Identities = 13/32 (40%), Positives = 18/32 (56%)
Frame = -3
Query: 310 DSKCLPPSSETPQLSKSCLKEFLNNLQPCSDE 215
D +CLPPS ET + +KE N ++ S E
Sbjct: 357 DMQCLPPSYETMGPCEKEMKEETNEVEIASIE 388
>SPBC18H10.07 |||WW domain-binding protein 4 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 224
Score = 25.8 bits (54), Expect = 6.7
Identities = 7/15 (46%), Positives = 11/15 (73%)
Frame = +2
Query: 347 YWESIPRSSCYFCSV 391
YW+SIP+ C +C +
Sbjct: 4 YWKSIPKYYCKYCQI 18
>SPCC594.06c |||SNARE Vam7 |Schizosaccharomyces pombe|chr 3|||Manual
Length = 341
Score = 25.8 bits (54), Expect = 6.7
Identities = 15/56 (26%), Positives = 25/56 (44%)
Frame = -3
Query: 382 KIAATSWNGFPITSRRPCATFHLVDSKCLPPSSETPQLSKSCLKEFLNNLQPCSDE 215
K+ + SW +T+ + + L + SETP + +K+FLN DE
Sbjct: 62 KLPSKSWVSSTVTNEKLRESRRLALQAYVQCLSETPWIKMPVVKKFLNIKDESEDE 117
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 25.4 bits (53), Expect = 8.9
Identities = 11/46 (23%), Positives = 21/46 (45%)
Frame = +3
Query: 378 IFVLYVEHLFVHFLHRHPSAENGSDGQISTMARVPGSHHVLGIKHL 515
+F+ + + LH H S+ +D I + V GS G++ +
Sbjct: 403 VFIEFNSDSGITSLHLHASSNTNADNIIRALGDVAGSARAAGLREI 448
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,252,115
Number of Sequences: 5004
Number of extensions: 69244
Number of successful extensions: 239
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 227
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 235
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 363302114
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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