Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= rbmte10l04
(857 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 394 e-110
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 215 7e-57
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 213 3e-56
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 152 5e-38
SPBC106.10 |pka1|tpk, git6|cAMP-dependent protein kinase catalyt... 28 2.0
SPBC19C7.03 |cyr1|git2|adenylate cyclase|Schizosaccharomyces pom... 27 2.6
SPAC222.07c |hri2||eIF2 alpha kinase Hri2|Schizosaccharomyces po... 26 7.9
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 394 bits (969), Expect = e-110
Identities = 177/260 (68%), Positives = 220/260 (84%)
Frame = -3
Query: 855 PSPTVSDTVXEPYNATLSVNQLIENSIQSYCIDNEALYYICHRTLKLMTPTYGDLNHLVS 676
P+P SDTV EPYNATLS++QL+ENS +++CIDNEAL I TLK+ +P+Y DLNHLVS
Sbjct: 171 PAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLVS 230
Query: 675 LTMSGVTTCLRFPGQLNADLRKLAVNMIPFPRLHFFMPGFAPLTSRNSQQYRALTVPELT 496
M+GVTT RFPG+LN+DLRKLAVNM+PFPRLHFFM GFAPL + S ++A++VPELT
Sbjct: 231 AVMAGVTTSFRFPGELNSDLRKLAVNMVPFPRLHFFMVGFAPLAAIGSSSFQAVSVPELT 290
Query: 495 QQMFDAKNMMAACDPHRGRYLTVATVFRGRMSMKEIDEQILNVQKKNKDFFVEWIPNNVQ 316
QQMFDA NMM A DP GRYLTVA +FRG++SMKE+DEQI +VQ KN +FVEWIP+NV
Sbjct: 291 QQMFDANNMMVAADPRHGRYLTVAALFRGKVSMKEVDEQIRSVQTKNSAYFVEWIPDNVL 350
Query: 315 TAVCDIPPRGMKMSATFIGNTTAIQEIFKRISEQFAAMFSRKAFLHWYTGEGMEEGDFAE 136
AVC +PP+ +KMSATFIGN+T+IQEIF+R+ +QF+AMF RKAFLHWYTGEGM+E +F E
Sbjct: 351 KAVCSVPPKDLKMSATFIGNSTSIQEIFRRLGDQFSAMFRRKAFLHWYTGEGMDEMEFTE 410
Query: 135 ADNNVSDLLSEYQQYQDATI 76
A++N++DL+SEYQQYQ+A I
Sbjct: 411 AESNMNDLVSEYQQYQEAGI 430
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 215 bits (525), Expect = 7e-57
Identities = 93/262 (35%), Positives = 158/262 (60%), Gaps = 8/262 (3%)
Frame = -3
Query: 855 PSPTVSDTVXEPYNATLSVNQLIENSIQSYCIDNEALYYICHRTLKLMTPTYGDLNHLVS 676
P+P VS +V EPYN+ L+ + ++NS ++ +DNEA Y IC R L + PTY +LN L++
Sbjct: 177 PAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIA 236
Query: 675 LTMSGVTTCLRFPGQLNADLRKLAVNMIPFPRLHFFMPGFAPLTSRNSQQYRALTVPELT 496
+S +T LRF G LN DL + N++P+PR+HF + ++P+ S + + +V E+T
Sbjct: 237 QVVSSITASLRFAGSLNVDLNEFQTNLVPYPRIHFPLVTYSPIVSAAKAFHESNSVQEIT 296
Query: 495 QQMFDAKNMMAACDPHRGRYLTVATVFRGRMSMKEIDEQILNVQKKNKDFFVEWIPNNVQ 316
Q F+ N M CDP GRY+ ++RG + +++ + +++ + FV+W P +
Sbjct: 297 NQCFEPYNQMVKCDPRTGRYMATCLLYRGDVIPRDVQAAVTSIKSRRTIQFVDWCPTGFK 356
Query: 315 TAVCDIPPR--------GMKMSATFIGNTTAIQEIFKRISEQFAAMFSRKAFLHWYTGEG 160
+C PP+ + + + NTT+I E + R+ +F M+S++AF+HWY GEG
Sbjct: 357 IGICYEPPQHVPGSGIAKVNRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEG 416
Query: 159 MEEGDFAEADNNVSDLLSEYQQ 94
MEEG+F+EA +++ L +Y++
Sbjct: 417 MEEGEFSEAREDLAALERDYEE 438
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 213 bits (520), Expect = 3e-56
Identities = 93/262 (35%), Positives = 158/262 (60%), Gaps = 8/262 (3%)
Frame = -3
Query: 855 PSPTVSDTVXEPYNATLSVNQLIENSIQSYCIDNEALYYICHRTLKLMTPTYGDLNHLVS 676
P+P VS +V EPYN+ L+ + ++ + ++ +DNE+ Y IC R L + P+Y +LN L++
Sbjct: 173 PAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIA 232
Query: 675 LTMSGVTTCLRFPGQLNADLRKLAVNMIPFPRLHFFMPGFAPLTSRNSQQYRALTVPELT 496
+S +T LRF G LN DL + N++P+PR+HF + +AP+ S + + +V E+T
Sbjct: 233 QVVSSITASLRFEGSLNVDLAEFQTNLVPYPRIHFPLVTYAPIVSAAKAFHESNSVQEIT 292
Query: 495 QQMFDAKNMMAACDPHRGRYLTVATVFRGRMSMKEIDEQILNVQKKNKDFFVEWIPNNVQ 316
Q F+ N M CDP GRY+ ++RG + +++ + ++ K FV+W P +
Sbjct: 293 NQCFEPYNQMVKCDPRAGRYMATCLLYRGDVIPRDVQAAVTTIKAKRTIQFVDWCPTGFK 352
Query: 315 TAVCDIPPRGMKMS--------ATFIGNTTAIQEIFKRISEQFAAMFSRKAFLHWYTGEG 160
+CD PP+ ++ S + NTT+I E + R+ +F M+S++AF+HWY GEG
Sbjct: 353 IGICDRPPQHIEGSEIAKVDRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEG 412
Query: 159 MEEGDFAEADNNVSDLLSEYQQ 94
MEEG+F+EA +++ L +Y++
Sbjct: 413 MEEGEFSEAREDLAALERDYEE 434
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 152 bits (369), Expect = 5e-38
Identities = 81/266 (30%), Positives = 146/266 (54%), Gaps = 10/266 (3%)
Frame = -3
Query: 852 SPTVSDTVXEPYNATLSVNQLIENSIQSYCIDNEALYYICHRTLKLMTPTYGDLNHLVSL 673
S +VSD V +PYN+ L++ +L N+ +DN AL +I L PT+ N LVS
Sbjct: 175 SQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVST 234
Query: 672 TMSGVTTCLRFPGQLNADLRKLAVNMIPFPRLHFFMPGFAPLTSRNSQQYRAL---TVPE 502
MS TT LR+PG +N DL + ++IP PR HF + + P T++ ++ +A+ TV +
Sbjct: 235 VMSASTTTLRYPGYMNNDLVSIIASLIPSPRCHFLLTSYTPFTNQQVEEAKAIRKTTVLD 294
Query: 501 LTQQMFDAKNMMAACDP-HRGRYLTVATVFRGRMSMKEIDEQILNVQKKNKDFFVEWIPN 325
+ +++ KN M + +P + ++++ + +G ++ + +L ++++ F+ W P
Sbjct: 295 VMRRLLLPKNQMVSVNPSKKSCFISILDIIQGEADPADVHKSLLRIRERRYASFIPWGPA 354
Query: 324 NVQTAVCDIPP---RGMKMSATFIGNTTAIQEIFKRISEQFAAMFSRKAFLHWYTGEGME 154
++Q A+ P ++S + N T+I +FKR +Q+ + R AFL Y E +
Sbjct: 355 SIQVALSKKSPYIKTNHRVSGLMLANHTSIASLFKRTLDQYDRLRKRNAFLEQYKKEAIF 414
Query: 153 EGDFAEADNN---VSDLLSEYQQYQD 85
E D E D++ V+DL++EY+ +D
Sbjct: 415 EDDLNEFDSSRDVVADLINEYEACED 440
>SPBC106.10 |pka1|tpk, git6|cAMP-dependent protein kinase catalytic
subunit Pka1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 512
Score = 27.9 bits (59), Expect = 2.0
Identities = 22/68 (32%), Positives = 32/68 (47%)
Frame = -2
Query: 625 RGSTKVSGQYDTLSEAALLYARLCTADVKK*SAVPRSHGTRINTTNVRRKEYDGGL*SSP 446
+GST V DTLS +A L+ + V + S R HGT N+ N + +D +
Sbjct: 9 KGSTNVGSSTDTLSTSASLHPSMNAGSVNEYSEQQR-HGT--NSFNGKPSVHDSVGSDAS 65
Query: 445 RSLSHCSH 422
S H +H
Sbjct: 66 VSNGHNNH 73
>SPBC19C7.03 |cyr1|git2|adenylate cyclase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1692
Score = 27.5 bits (58), Expect = 2.6
Identities = 13/36 (36%), Positives = 21/36 (58%)
Frame = -3
Query: 792 LIENSIQSYCIDNEALYYICHRTLKLMTPTYGDLNH 685
L NS++ I N L+++ H T L+ TY DL++
Sbjct: 499 LSRNSLKELNIANNKLFFLPHSTRYLVNLTYLDLSY 534
>SPAC222.07c |hri2||eIF2 alpha kinase Hri2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 639
Score = 25.8 bits (54), Expect = 7.9
Identities = 10/22 (45%), Positives = 13/22 (59%)
Frame = +1
Query: 391 FLH*HSSPEDGGYSEIATAVRI 456
F + H S EDG YSE A+ +
Sbjct: 3 FFNAHKSAEDGNYSETTNAISL 24
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,367,067
Number of Sequences: 5004
Number of extensions: 70963
Number of successful extensions: 221
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 212
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 218
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 426466470
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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