Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= rbmte10k19
(892 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 437 e-124
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 229 3e-61
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 228 9e-61
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 152 6e-38
SPBP4H10.04 |ppb1||calcineurin catalytic subunit Ppb1|Schizosacc... 29 0.89
SPBC4F6.07c |||ATP-dependent RNA helicase Mak5 |Schizosaccharomy... 27 2.7
SPBC557.04 |ppk29||Ark1/Prk1 family protein kinase Ppk29|Schizos... 27 3.6
SPBC16E9.14c |||membrane transporter|Schizosaccharomyces pombe|c... 27 3.6
SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyce... 27 4.7
SPBC27B12.05 |||WD repeat protein|Schizosaccharomyces pombe|chr ... 27 4.7
SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein Ubr1|Sc... 26 6.3
SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal |Schizos... 26 6.3
SPBC211.03c |||guanyl-nucleotide exchange factor|Schizosaccharom... 26 6.3
SPAC140.03 |arb1||argonaute binding protein 1|Schizosaccharomyce... 26 6.3
SPBC19C7.12c |||alpha-1,2-mannosyltransferase|Schizosaccharomyce... 26 8.3
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 26 8.3
SPAC56F8.12 |||conserved fungal protein|Schizosaccharomyces pomb... 26 8.3
SPBC18H10.07 |||WW domain-binding protein 4 |Schizosaccharomyces... 26 8.3
SPCC594.06c |||SNARE Vam7 |Schizosaccharomyces pombe|chr 3|||Manual 26 8.3
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 437 bits (1077), Expect = e-124
Identities = 199/258 (77%), Positives = 230/258 (89%)
Frame = -3
Query: 887 PVQKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVS 708
P K SDTVVEPYNATLS+HQLVEN+DET+CIDNEAL I TLK+ +P+Y DLNHLVS
Sbjct: 171 PAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLVS 230
Query: 707 ATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALSVPELT 528
A M+GVTT RFPG+LN+DLRKLAVNMVPFPRLHFFM GFAPL + GS ++A+SVPELT
Sbjct: 231 AVMAGVTTSFRFPGELNSDLRKLAVNMVPFPRLHFFMVGFAPLAAIGSSSFQAVSVPELT 290
Query: 527 QQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNIQNKNSSYFVEWIPNNVK 348
QQMFDA NMM A DPRHGRYLTVAA+FRG++SMKEVDEQ+ ++Q KNS+YFVEWIP+NV
Sbjct: 291 QQMFDANNMMVAADPRHGRYLTVAALFRGKVSMKEVDEQIRSVQTKNSAYFVEWIPDNVL 350
Query: 347 TAVCDIPPRGLKMSATFIGNTTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTE 168
AVC +PP+ LKMSATFIGN+T+IQE+F+R+ +QF+AMFRRKAFLHWYTGEGMDEMEFTE
Sbjct: 351 KAVCSVPPKDLKMSATFIGNSTSIQEIFRRLGDQFSAMFRRKAFLHWYTGEGMDEMEFTE 410
Query: 167 AESNMNDLVSEYQQYQDA 114
AESNMNDLVSEYQQYQ+A
Sbjct: 411 AESNMNDLVSEYQQYQEA 428
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 229 bits (561), Expect = 3e-61
Identities = 101/263 (38%), Positives = 161/263 (61%), Gaps = 8/263 (3%)
Frame = -3
Query: 890 YPVQKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLV 711
YP +VS +VVEPYN+ L+ H ++N+D T+ +DNEA YDIC R L + PTY +LN L+
Sbjct: 176 YPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLI 235
Query: 710 SATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALSVPEL 531
+ +S +T LRF G LN DL + N+VP+PR+HF + ++P+ S + + SV E+
Sbjct: 236 AQVVSSITASLRFAGSLNVDLNEFQTNLVPYPRIHFPLVTYSPIVSAAKAFHESNSVQEI 295
Query: 530 TQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNIQNKNSSYFVEWIPNNV 351
T Q F+ N M CDPR GRY+ ++RG + ++V + +I+++ + FV+W P
Sbjct: 296 TNQCFEPYNQMVKCDPRTGRYMATCLLYRGDVIPRDVQAAVTSIKSRRTIQFVDWCPTGF 355
Query: 350 KTAVCDIPPR--------GLKMSATFIGNTTAIQELFKRISEQFTAMFRRKAFLHWYTGE 195
K +C PP+ + + + NTT+I E + R+ +F M+ ++AF+HWY GE
Sbjct: 356 KIGICYEPPQHVPGSGIAKVNRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGE 415
Query: 194 GMDEMEFTEAESNMNDLVSEYQQ 126
GM+E EF+EA ++ L +Y++
Sbjct: 416 GMEEGEFSEAREDLAALERDYEE 438
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 228 bits (557), Expect = 9e-61
Identities = 102/263 (38%), Positives = 159/263 (60%), Gaps = 8/263 (3%)
Frame = -3
Query: 890 YPVQKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLV 711
YP +VS +VVEPYN+ L+ H ++ D T+ +DNE+ YDIC R L + P+Y +LN L+
Sbjct: 172 YPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLI 231
Query: 710 SATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALSVPEL 531
+ +S +T LRF G LN DL + N+VP+PR+HF + +AP+ S + + SV E+
Sbjct: 232 AQVVSSITASLRFEGSLNVDLAEFQTNLVPYPRIHFPLVTYAPIVSAAKAFHESNSVQEI 291
Query: 530 TQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNIQNKNSSYFVEWIPNNV 351
T Q F+ N M CDPR GRY+ ++RG + ++V + I+ K + FV+W P
Sbjct: 292 TNQCFEPYNQMVKCDPRAGRYMATCLLYRGDVIPRDVQAAVTTIKAKRTIQFVDWCPTGF 351
Query: 350 KTAVCDIPPRGLKMS--------ATFIGNTTAIQELFKRISEQFTAMFRRKAFLHWYTGE 195
K +CD PP+ ++ S + NTT+I E + R+ +F M+ ++AF+HWY GE
Sbjct: 352 KIGICDRPPQHIEGSEIAKVDRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGE 411
Query: 194 GMDEMEFTEAESNMNDLVSEYQQ 126
GM+E EF+EA ++ L +Y++
Sbjct: 412 GMEEGEFSEAREDLAALERDYEE 434
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 152 bits (369), Expect = 6e-38
Identities = 81/265 (30%), Positives = 145/265 (54%), Gaps = 10/265 (3%)
Frame = -3
Query: 881 QKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSAT 702
Q VSD VV+PYN+ L++ +L N D +DN AL I L PT+ N LVS
Sbjct: 176 QSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVSTV 235
Query: 701 MSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRAL---SVPEL 531
MS TT LR+PG +N DL + +++P PR HF + + P T++ ++ +A+ +V ++
Sbjct: 236 MSASTTTLRYPGYMNNDLVSIIASLIPSPRCHFLLTSYTPFTNQQVEEAKAIRKTTVLDV 295
Query: 530 TQQMFDAKNMMAACDP-RHGRYLTVAAVFRGRMSMKEVDEQMLNIQNKNSSYFVEWIPNN 354
+++ KN M + +P + ++++ + +G +V + +L I+ + + F+ W P +
Sbjct: 296 MRRLLLPKNQMVSVNPSKKSCFISILDIIQGEADPADVHKSLLRIRERRYASFIPWGPAS 355
Query: 353 VKTAVCDIPP---RGLKMSATFIGNTTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDE 183
++ A+ P ++S + N T+I LFKR +Q+ + +R AFL Y E + E
Sbjct: 356 IQVALSKKSPYIKTNHRVSGLMLANHTSIASLFKRTLDQYDRLRKRNAFLEQYKKEAIFE 415
Query: 182 MEFTEAESNMN---DLVSEYQQYQD 117
+ E +S+ + DL++EY+ +D
Sbjct: 416 DDLNEFDSSRDVVADLINEYEACED 440
>SPBP4H10.04 |ppb1||calcineurin catalytic subunit
Ppb1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 554
Score = 29.1 bits (62), Expect = 0.89
Identities = 16/66 (24%), Positives = 33/66 (50%), Gaps = 2/66 (3%)
Frame = -3
Query: 452 VFRGRMSMKEVDEQMLNIQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIG--NTTA 279
V+ + ++ + + ++NI+ N S W+PN + +P G K+S I N +
Sbjct: 343 VYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVSEMLISMLNICS 402
Query: 278 IQELFK 261
+EL++
Sbjct: 403 KEELYE 408
>SPBC4F6.07c |||ATP-dependent RNA helicase Mak5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 648
Score = 27.5 bits (58), Expect = 2.7
Identities = 13/46 (28%), Positives = 23/46 (50%)
Frame = +3
Query: 258 SFKQLLDSCGVSDEGGRHFESTRWNVAHGRLDVIGNPFHEVAAIFV 395
+FK++ C V DE R + + + L+++GNP H F+
Sbjct: 265 NFKKI--KCLVLDEADRLLQKSHFEELSKLLEILGNPMHTQRQTFI 308
>SPBC557.04 |ppk29||Ark1/Prk1 family protein kinase
Ppk29|Schizosaccharomyces pombe|chr 2|||Manual
Length = 872
Score = 27.1 bits (57), Expect = 3.6
Identities = 13/36 (36%), Positives = 21/36 (58%)
Frame = -3
Query: 290 NTTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDE 183
N++ IQ L K I+ T +R ++ Y+G G+DE
Sbjct: 193 NSSEIQALEKSINTFTTYQYRAPEMINLYSGLGIDE 228
>SPBC16E9.14c |||membrane transporter|Schizosaccharomyces pombe|chr
2|||Manual
Length = 386
Score = 27.1 bits (57), Expect = 3.6
Identities = 19/64 (29%), Positives = 33/64 (51%), Gaps = 3/64 (4%)
Frame = +2
Query: 710 KPNGSSPHTLGLLASKS*SRYRKAPHCLYNTSHQCFLPIGGLIALRYMA---LPQYRKPF 880
KP+ ++P+ LL+ + +RK H ++ SHQ FLP L A +P +++ F
Sbjct: 31 KPSANNPN---LLSVDTRPTHRKGHHHKHSLSHQYFLPPKNRQPLEIPASYPIPTFKETF 87
Query: 881 GLGT 892
+ T
Sbjct: 88 AILT 91
>SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1242
Score = 26.6 bits (56), Expect = 4.7
Identities = 15/40 (37%), Positives = 23/40 (57%)
Frame = +3
Query: 75 LYLFIKFPFIISRGVLILLVLRNQIVHVRFSLRELHFIHT 194
LY+ I F I +RG L L +N ++ + F LR ++F T
Sbjct: 1075 LYVAIPFRTIFNRGTLALDAFQNWVIGL-FMLRMIYFTVT 1113
>SPBC27B12.05 |||WD repeat protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 391
Score = 26.6 bits (56), Expect = 4.7
Identities = 10/23 (43%), Positives = 15/23 (65%)
Frame = -3
Query: 725 LNHLVSATMSGVTTCLRFPGQLN 657
L HLV A + G++ C +PG+ N
Sbjct: 331 LTHLVDAPLLGMSVCPLYPGEEN 353
>SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein
Ubr1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1958
Score = 26.2 bits (55), Expect = 6.3
Identities = 18/59 (30%), Positives = 29/59 (49%)
Frame = -3
Query: 296 IGNTTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ 120
+ N + L+K + E+F+ +F RK L WY G+ E + N+N SE + Q
Sbjct: 1703 LNNPHLLFTLYKLL-ERFSLIFLRKCALLWYCRYGVS----FETQPNLNFQNSELSRLQ 1756
>SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 647
Score = 26.2 bits (55), Expect = 6.3
Identities = 18/73 (24%), Positives = 35/73 (47%)
Frame = -1
Query: 301 PSSETPQLSKSCLKEFLNNLQPCSDEKLSCTGTRGRVWMKWSSRRLNRT*TIWFRSTSSI 122
PSS+ ++ K L F+ N+ ++ T +VW+ SS L+ T W +S++
Sbjct: 562 PSSDCSKILKCLLDGFVRNVAHLQNDGSYKTIGGKQVWLD-SSSVLHEKKTPWIMYSSAV 620
Query: 121 KTPRLMMKGNLMK 83
++ + N+ K
Sbjct: 621 ESETQIFVKNISK 633
>SPBC211.03c |||guanyl-nucleotide exchange factor|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1462
Score = 26.2 bits (55), Expect = 6.3
Identities = 13/49 (26%), Positives = 22/49 (44%), Gaps = 3/49 (6%)
Frame = +3
Query: 204 VPVQESFSSEHGCKL---FRNSFKQLLDSCGVSDEGGRHFESTRWNVAH 341
+P +S H +L F K ++ S+E HF+ TRW + +
Sbjct: 1216 LPFDKSILDNHSAELNDAFNLFLKAAVEYKVDSNESSEHFQDTRWKIIY 1264
>SPAC140.03 |arb1||argonaute binding protein 1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 399
Score = 26.2 bits (55), Expect = 6.3
Identities = 14/44 (31%), Positives = 24/44 (54%), Gaps = 2/44 (4%)
Frame = -3
Query: 473 RYLTVAAVFRGRMSMKEVDEQMLNIQNKN--SSYFVEWIPNNVK 348
RYLT V + +++K V + +LN N++ + F+ W P K
Sbjct: 297 RYLTGKVVEQEYLTVKLVSKTLLNFSNQSLCKAVFIVWDPPGSK 340
>SPBC19C7.12c |||alpha-1,2-mannosyltransferase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 390
Score = 25.8 bits (54), Expect = 8.3
Identities = 12/39 (30%), Positives = 17/39 (43%)
Frame = -3
Query: 473 RYLTVAAVFRGRMSMKEVDEQMLNIQNKNSSYFVEWIPN 357
+Y+ +VF G + NI N S EW+PN
Sbjct: 29 QYIPTISVFEGSLIDNRDTLSYFNISNLEPSERSEWLPN 67
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 25.8 bits (54), Expect = 8.3
Identities = 11/46 (23%), Positives = 21/46 (45%)
Frame = +3
Query: 387 IFVLYVEHLFVHFLHRHPSAENGSDGQISTMARVTGSHHVLGIKHL 524
+F+ + + LH H S+ +D I + V GS G++ +
Sbjct: 403 VFIEFNSDSGITSLHLHASSNTNADNIIRALGDVAGSARAAGLREI 448
>SPAC56F8.12 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 394
Score = 25.8 bits (54), Expect = 8.3
Identities = 13/32 (40%), Positives = 18/32 (56%)
Frame = -1
Query: 319 DSKCLPPSSETPQLSKSCLKEFLNNLQPCSDE 224
D +CLPPS ET + +KE N ++ S E
Sbjct: 357 DMQCLPPSYETMGPCEKEMKEETNEVEIASIE 388
>SPBC18H10.07 |||WW domain-binding protein 4 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 224
Score = 25.8 bits (54), Expect = 8.3
Identities = 7/15 (46%), Positives = 11/15 (73%)
Frame = +2
Query: 356 YWESIPRSSCYFCSV 400
YW+SIP+ C +C +
Sbjct: 4 YWKSIPKYYCKYCQI 18
>SPCC594.06c |||SNARE Vam7 |Schizosaccharomyces pombe|chr 3|||Manual
Length = 341
Score = 25.8 bits (54), Expect = 8.3
Identities = 15/56 (26%), Positives = 25/56 (44%)
Frame = -1
Query: 391 KIAATSWNGFPITSRRPCATFHLVDSKCLPPSSETPQLSKSCLKEFLNNLQPCSDE 224
K+ + SW +T+ + + L + SETP + +K+FLN DE
Sbjct: 62 KLPSKSWVSSTVTNEKLRESRRLALQAYVQCLSETPWIKMPVVKKFLNIKDESEDE 117
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,846,905
Number of Sequences: 5004
Number of extensions: 83350
Number of successful extensions: 287
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 274
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 283
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 448490560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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