Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= rbmte10c24
         (826 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC222.12c |atp2||F1-ATPase beta subunit |Schizosaccharomyces p...   168   1e-42
SPAC637.05c |vma2||V-type ATPase V1 subunit B |Schizosaccharomyc...    31   0.15 
SPBC543.09 |||mitochondrial m-AAA protease|Schizosaccharomyces p...    29   0.80 
SPBC725.11c |php2||CCAAT-binding factor complex subunit Php2 |Sc...    29   0.80 
SPCC74.02c |||mRNA cleavage and polyadenylation specificity fact...    26   5.6  
SPBC3B8.08 |||Sjogren's syndrome/scleroderma autoantigen 1 famil...    25   9.9  

>SPAC222.12c |atp2||F1-ATPase beta subunit |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 525

 Score =  168 bits (408), Expect = 1e-42
 Identities = 91/150 (60%), Positives = 111/150 (74%), Gaps = 14/150 (9%)
 Frame = -1

Query: 598 LDRVTLAEHFRDEEGQDLLLFIDNIFRFTQAGSEV-----RTRSSYQFE-----GRGGR- 452
           L  +T+AE+FRD EGQD+LLFIDNIFRFTQAGSEV     R  S+  ++       G   
Sbjct: 280 LTGLTVAEYFRDIEGQDVLLFIDNIFRFTQAGSEVSALLGRIPSAVGYQPTLATDMGAMQ 339

Query: 451 ---FTVKLSLRSNLKAVYVPADDLTDPAPATTFAHLDATTVLSPAIAELGVYPAVDPLDS 281
               T K    ++++AVYVPADDLTDPAPATTFAHLDATTVLS +I+ELG+YPAVDPLDS
Sbjct: 340 ERITTTKKGSITSVQAVYVPADDLTDPAPATTFAHLDATTVLSRSISELGIYPAVDPLDS 399

Query: 280 TSRVMDPNIIGAEHYGIARGVQKILQAWKS 191
            SR+MDP I+G EHY +A  VQ++LQ +KS
Sbjct: 400 KSRMMDPRILGEEHYNLAGSVQQMLQEYKS 429



 Score = 41.5 bits (93), Expect = 1e-04
 Identities = 19/27 (70%), Positives = 24/27 (88%)
 Frame = -2

Query: 669 ELETRASLVYGQKDEPHGARARVALTG 589
           E E++A+LV+GQ +EP GARARVALTG
Sbjct: 256 EGESKAALVFGQMNEPPGARARVALTG 282



 Score = 39.1 bits (87), Expect = 7e-04
 Identities = 17/29 (58%), Positives = 22/29 (75%)
 Frame = -3

Query: 203 GMEELSEDDQPEIARARKIQRFPSQPIQI 117
           GM+ELSE D+  + RARK+QRF SQP  +
Sbjct: 438 GMDELSEADKLTVERARKVQRFLSQPFAV 466



 Score = 34.3 bits (75), Expect = 0.021
 Identities = 15/29 (51%), Positives = 23/29 (79%), Gaps = 3/29 (10%)
 Frame = -2

Query: 102 DHIPEAAFYMVGTIEDVI---AKLSQELS 25
           D +PE+AFYMVG+I+D +    K++QEL+
Sbjct: 496 DSLPESAFYMVGSIDDAVKKAEKIAQELA 524


>SPAC637.05c |vma2||V-type ATPase V1 subunit B |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 503

 Score = 31.5 bits (68), Expect = 0.15
 Identities = 17/66 (25%), Positives = 30/66 (45%)
 Frame = -1

Query: 463 RGGRFTVKLSLRSNLKAVYVPADDLTDPAPATTFAHLDATTVLSPAIAELGVYPAVDPLD 284
           R GR   +    + +  + +P DD+T P P  T    +    +   +    +YP ++ L 
Sbjct: 315 RAGRVEGRNGSITQIPILTMPNDDITHPIPDLTGYITEGQIFVDRQLHNNAIYPPINVLP 374

Query: 283 STSRVM 266
           S SR+M
Sbjct: 375 SLSRLM 380


>SPBC543.09 |||mitochondrial m-AAA protease|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 773

 Score = 29.1 bits (62), Expect = 0.80
 Identities = 17/52 (32%), Positives = 21/52 (40%)
 Frame = -1

Query: 466 GRGGRFTVKLSLRSNLKAVYVPADDLTDPAPATTFAHLDATTVLSPAIAELG 311
           GRGG+F       S L  + V  D  T       FA  +   VL PA+   G
Sbjct: 405 GRGGQFGSNDERESTLNQLLVEMDGFTSSEHIVVFAGTNRPDVLDPALLRPG 456


>SPBC725.11c |php2||CCAAT-binding factor complex subunit Php2
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 334

 Score = 29.1 bits (62), Expect = 0.80
 Identities = 19/49 (38%), Positives = 23/49 (46%), Gaps = 2/49 (4%)
 Frame = -1

Query: 376 PATTFAHLDATTVLSPAIAELGVYPA--VDPLDSTSRVMDPNIIGAEHY 236
           P  +  HLD   V  P    L V PA  +DPL+ T    D  IIG + Y
Sbjct: 279 PMDSIDHLD---VNDPTATGLPVLPASDIDPLNLTGNTQDSMIIGQQTY 324


>SPCC74.02c |||mRNA cleavage and polyadenylation specificity factor
           complex associated protein|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 710

 Score = 26.2 bits (55), Expect = 5.6
 Identities = 16/47 (34%), Positives = 21/47 (44%)
 Frame = -2

Query: 261 LTSSGLSITASPGACRKYCRHGRAVRR*PARDSQSQEDTEVPFAADP 121
           L SSG S T SP       +   +    P+ DSQSQ       +A+P
Sbjct: 91  LISSGSSQTGSPSQSLSSNKEPSSPGISPSNDSQSQNTNHTSISANP 137


>SPBC3B8.08 |||Sjogren's syndrome/scleroderma autoantigen 1
           family|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 153

 Score = 25.4 bits (53), Expect = 9.9
 Identities = 10/27 (37%), Positives = 14/27 (51%)
 Frame = +3

Query: 27  RVPVTTWRSRPLWCPPCRRQLPECDPS 107
           +VP+   R  P++C  C   L E D S
Sbjct: 31  KVPMMRLRDNPMFCVSCINDLKESDAS 57


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,985,436
Number of Sequences: 5004
Number of extensions: 55949
Number of successful extensions: 159
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 151
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 159
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 404442380
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)