Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= rbmte10c03
(492 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein Ubi2|... 216 2e-57
SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein Ubi1|Schiz... 216 2e-57
SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr 2|||M... 151 6e-38
SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein Ubi3|Schi... 150 1e-37
SPAC589.10c |||ribomal-ubiquitin fusion protein Ubi5|Schizosacch... 150 1e-37
SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like pro... 90 2e-19
SPBC2D10.12 |rhp23||Rad23 homolog Rhp23|Schizosaccharomyces pomb... 42 7e-05
SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyc... 40 2e-04
SPAC6G9.08 |ubp6||ubiquitin C-terminal hydrolase Ubp6|Schizosacc... 38 6e-04
SPBC1E8.02 |||ubiquitin family protein, unknown|Schizosaccharomy... 27 1.2
SPBC31E1.03 |hub1|ubl4|ubiquitin-like protein modifier Hub1|Schi... 27 2.0
SPBC776.09 |ste13||ATP-dependent RNA helicase Ste13|Schizosaccha... 25 4.7
SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces... 25 6.2
SPBC418.01c |his4|SPBC887.20c|imidazoleglycerol-phosphate syntha... 25 6.2
SPAC31G5.13 |rpn11|pad1, sks1, bfr2, mts5|19S proteasome regulat... 25 8.2
SPCC1682.07 |ssl1||transcription factor TFIIH complex subunit Ss... 25 8.2
SPBC21C3.20c |git1||C2 domain protein Git1|Schizosaccharomyces p... 25 8.2
SPAC19A8.12 |dcp2||mRNA decapping complex subunit Dcp2|Schizosac... 25 8.2
>SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein
Ubi2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 128
Score = 216 bits (527), Expect = 2e-57
Identities = 102/112 (91%), Positives = 106/112 (94%)
Frame = -3
Query: 427 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQXRLIFAGKQLEDGRTLSDYN 248
MQIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQ RLIFAGKQLEDGRTLSDYN
Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60
Query: 247 IQKESTLHLVLRLRGGTIEPSLRILAMKYNCEKMICRKCYARLHPRATNCRK 92
IQKESTLHLVLRLRGG IEPSL+ LA KYNCEK ICRKCYARL PRATNCRK
Sbjct: 61 IQKESTLHLVLRLRGGIIEPSLKALASKYNCEKQICRKCYARLPPRATNCRK 112
Score = 25.0 bits (52), Expect = 6.2
Identities = 8/12 (66%), Positives = 10/12 (83%)
Frame = -1
Query: 93 RQTCGHTNNLRP 58
++ CGHTN LRP
Sbjct: 112 KKKCGHTNQLRP 123
>SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein
Ubi1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 128
Score = 216 bits (527), Expect = 2e-57
Identities = 102/112 (91%), Positives = 106/112 (94%)
Frame = -3
Query: 427 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQXRLIFAGKQLEDGRTLSDYN 248
MQIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQ RLIFAGKQLEDGRTLSDYN
Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60
Query: 247 IQKESTLHLVLRLRGGTIEPSLRILAMKYNCEKMICRKCYARLHPRATNCRK 92
IQKESTLHLVLRLRGG IEPSL+ LA KYNCEK ICRKCYARL PRATNCRK
Sbjct: 61 IQKESTLHLVLRLRGGIIEPSLKALASKYNCEKQICRKCYARLPPRATNCRK 112
Score = 25.0 bits (52), Expect = 6.2
Identities = 8/12 (66%), Positives = 10/12 (83%)
Frame = -1
Query: 93 RQTCGHTNNLRP 58
++ CGHTN LRP
Sbjct: 112 KKKCGHTNQLRP 123
>SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 382
Score = 151 bits (366), Expect = 6e-38
Identities = 75/90 (83%), Positives = 81/90 (90%)
Frame = -3
Query: 469 KKS*ILRVVYFSNTMQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQXRLIFA 290
K+S + V+ MQIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQ RLIFA
Sbjct: 63 KESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFA 122
Query: 289 GKQLEDGRTLSDYNIQKESTLHLVLRLRGG 200
GKQLEDGRTLSDYNIQKESTLHLVLRLRGG
Sbjct: 123 GKQLEDGRTLSDYNIQKESTLHLVLRLRGG 152
Score = 151 bits (366), Expect = 6e-38
Identities = 75/90 (83%), Positives = 81/90 (90%)
Frame = -3
Query: 469 KKS*ILRVVYFSNTMQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQXRLIFA 290
K+S + V+ MQIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQ RLIFA
Sbjct: 139 KESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFA 198
Query: 289 GKQLEDGRTLSDYNIQKESTLHLVLRLRGG 200
GKQLEDGRTLSDYNIQKESTLHLVLRLRGG
Sbjct: 199 GKQLEDGRTLSDYNIQKESTLHLVLRLRGG 228
Score = 151 bits (366), Expect = 6e-38
Identities = 75/90 (83%), Positives = 81/90 (90%)
Frame = -3
Query: 469 KKS*ILRVVYFSNTMQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQXRLIFA 290
K+S + V+ MQIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQ RLIFA
Sbjct: 215 KESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFA 274
Query: 289 GKQLEDGRTLSDYNIQKESTLHLVLRLRGG 200
GKQLEDGRTLSDYNIQKESTLHLVLRLRGG
Sbjct: 275 GKQLEDGRTLSDYNIQKESTLHLVLRLRGG 304
Score = 151 bits (366), Expect = 6e-38
Identities = 75/90 (83%), Positives = 81/90 (90%)
Frame = -3
Query: 469 KKS*ILRVVYFSNTMQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQXRLIFA 290
K+S + V+ MQIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQ RLIFA
Sbjct: 291 KESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFA 350
Query: 289 GKQLEDGRTLSDYNIQKESTLHLVLRLRGG 200
GKQLEDGRTLSDYNIQKESTLHLVLRLRGG
Sbjct: 351 GKQLEDGRTLSDYNIQKESTLHLVLRLRGG 380
Score = 150 bits (363), Expect = 1e-37
Identities = 72/76 (94%), Positives = 75/76 (98%)
Frame = -3
Query: 427 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQXRLIFAGKQLEDGRTLSDYN 248
MQIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQ RLIFAGKQLEDGRTLSDYN
Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60
Query: 247 IQKESTLHLVLRLRGG 200
IQKESTLHLVLRLRGG
Sbjct: 61 IQKESTLHLVLRLRGG 76
>SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein
Ubi3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 150
Score = 150 bits (363), Expect = 1e-37
Identities = 72/76 (94%), Positives = 75/76 (98%)
Frame = -3
Query: 427 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQXRLIFAGKQLEDGRTLSDYN 248
MQIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQ RLIFAGKQLEDGRTLSDYN
Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60
Query: 247 IQKESTLHLVLRLRGG 200
IQKESTLHLVLRLRGG
Sbjct: 61 IQKESTLHLVLRLRGG 76
>SPAC589.10c |||ribomal-ubiquitin fusion protein
Ubi5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 150
Score = 150 bits (363), Expect = 1e-37
Identities = 72/76 (94%), Positives = 75/76 (98%)
Frame = -3
Query: 427 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQXRLIFAGKQLEDGRTLSDYN 248
MQIFVKTLTGKTITLEVE+SDTI+NVK+KIQDKEGIPPDQ RLIFAGKQLEDGRTLSDYN
Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60
Query: 247 IQKESTLHLVLRLRGG 200
IQKESTLHLVLRLRGG
Sbjct: 61 IQKESTLHLVLRLRGG 76
>SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like
protein modifier Ned8|Schizosaccharomyces pombe|chr
2|||Manual
Length = 78
Score = 89.8 bits (213), Expect = 2e-19
Identities = 39/77 (50%), Positives = 56/77 (72%)
Frame = -3
Query: 427 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQXRLIFAGKQLEDGRTLSDYN 248
M I VKTLTGK I L+++ +D + +K ++++KEGIPP Q RLI+AGKQ+ D + Y+
Sbjct: 1 MLIKVKTLTGKEIELDIDPNDKVSRIKERVEEKEGIPPSQQRLIYAGKQMADDKNAESYH 60
Query: 247 IQKESTLHLVLRLRGGT 197
++ S LHLVL LRGG+
Sbjct: 61 LEGGSVLHLVLALRGGS 77
>SPBC2D10.12 |rhp23||Rad23 homolog Rhp23|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 368
Score = 41.5 bits (93), Expect = 7e-05
Identities = 21/71 (29%), Positives = 41/71 (57%), Gaps = 1/71 (1%)
Frame = -3
Query: 397 KTITLEVEASDTIENVKAKIQDKEGIPPDQXRLIFAGKQLEDGRTLSDYNIQKES-TLHL 221
K + +V A I +K KIQ ++ ++ +LI++G+ L D +T+ +YNI+++ + +
Sbjct: 12 KFVISDVSADTKISELKEKIQTQQNYEVERQKLIYSGRILADDKTVGEYNIKEQDFIVCM 71
Query: 220 VLRLRGGTIEP 188
V R + T P
Sbjct: 72 VSRPKTSTSTP 82
>SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 354
Score = 39.9 bits (89), Expect = 2e-04
Identities = 20/73 (27%), Positives = 38/73 (52%)
Frame = -3
Query: 427 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQXRLIFAGKQLEDGRTLSDYN 248
+ + +K + + V++ ++ +K I I ++ RLI+AG+ L+D +L Y
Sbjct: 4 ISLTIKAANDQKYAVTVDSESSVLALKEAIAPVADIEKERQRLIYAGRVLKDEESLKTYK 63
Query: 247 IQKESTLHLVLRL 209
IQ ++HLV L
Sbjct: 64 IQDGHSIHLVKTL 76
>SPAC6G9.08 |ubp6||ubiquitin C-terminal hydrolase
Ubp6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 467
Score = 38.3 bits (85), Expect = 6e-04
Identities = 18/58 (31%), Positives = 32/58 (55%)
Frame = -3
Query: 400 GKTITLEVEASDTIENVKAKIQDKEGIPPDQXRLIFAGKQLEDGRTLSDYNIQKESTL 227
GK LE+E ++T +K ++ +PP++ ++I G QL+D L I+ +TL
Sbjct: 10 GKKYDLEIEPNETGSTLKHQLYSLTQVPPERQKVIVKGGQLKDDVLLGSVGIKPNATL 67
>SPBC1E8.02 |||ubiquitin family protein, unknown|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 603
Score = 27.5 bits (58), Expect = 1.2
Identities = 21/78 (26%), Positives = 36/78 (46%), Gaps = 2/78 (2%)
Frame = -3
Query: 424 QIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQXRLIFAGKQLEDGRTLSD--Y 251
+I V T+ K +V + T+ +K I P D+ +LI AG+ L + L + +
Sbjct: 5 RIRVTTVDQKVGIFQVPRTKTVLELKELIAVTFEAPADRLKLIHAGRVLRNETPLEEILH 64
Query: 250 NIQKESTLHLVLRLRGGT 197
+ T HLV+ + T
Sbjct: 65 DATDLVTFHLVIAVFNST 82
>SPBC31E1.03 |hub1|ubl4|ubiquitin-like protein modifier
Hub1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 73
Score = 26.6 bits (56), Expect = 2.0
Identities = 15/69 (21%), Positives = 30/69 (43%)
Frame = -3
Query: 427 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQXRLIFAGKQLEDGRTLSDYN 248
+++ GK + ++ DT+ + K + + G P + L +D TL+DY
Sbjct: 2 IEVLCNDRLGKKVRVKCMPDDTVGDFKKLVAAQTGTDPRRIVLKKWHSVFKDNITLADYE 61
Query: 247 IQKESTLHL 221
I +L +
Sbjct: 62 IHDGMSLEM 70
>SPBC776.09 |ste13||ATP-dependent RNA helicase
Ste13|Schizosaccharomyces pombe|chr 2|||Manual
Length = 485
Score = 25.4 bits (53), Expect = 4.7
Identities = 27/116 (23%), Positives = 48/116 (41%), Gaps = 13/116 (11%)
Frame = -3
Query: 412 KTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQXRLIFA------GKQLE-------D 272
K TGKT + + + ++ K+KIQ +P + L + GK +
Sbjct: 89 KNGTGKTAAFVIPSLEKVDTKKSKIQTLILVPTRELALQTSQVCKTLGKHMNVKVMVTTG 148
Query: 271 GRTLSDYNIQKESTLHLVLRLRGGTIEPSLRILAMKYNCEKMICRKCYARLHPRAT 104
G TL D I+ T+H+V+ G ++ + + +A C + + L P T
Sbjct: 149 GTTLRDDIIRLNDTVHIVVGTPGRVLDLAGKGVADFSECTTFVMDEADKLLSPEFT 204
>SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3131
Score = 25.0 bits (52), Expect = 6.2
Identities = 10/27 (37%), Positives = 16/27 (59%)
Frame = +2
Query: 347 FDIFDSVGSFHLQCNGLPRKGFDEYLH 427
F ++S+G + L C + FDEYL+
Sbjct: 2887 FKPYESLGKYMLSCLDDGKYAFDEYLY 2913
>SPBC418.01c |his4|SPBC887.20c|imidazoleglycerol-phosphate
synthase|Schizosaccharomyces pombe|chr 2|||Manual
Length = 541
Score = 25.0 bits (52), Expect = 6.2
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = +2
Query: 353 IFDSVGSFHLQCNGLPRKGFDEYL 424
IF VG+F C+ L ++GF E L
Sbjct: 43 IFPGVGNFGFVCDSLAKQGFLEPL 66
>SPAC31G5.13 |rpn11|pad1, sks1, bfr2, mts5|19S proteasome regulatory
subunit Rpn11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 308
Score = 24.6 bits (51), Expect = 8.2
Identities = 12/40 (30%), Positives = 22/40 (55%)
Frame = -3
Query: 358 ENVKAKIQDKEGIPPDQXRLIFAGKQLEDGRTLSDYNIQK 239
E ++Q++ + P+Q R+ + GKQ D + D +QK
Sbjct: 252 EQYTERVQNEVTLSPEQLRIQYVGKQ--DPKKHLDAEVQK 289
>SPCC1682.07 |ssl1||transcription factor TFIIH complex subunit
Ssl1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 421
Score = 24.6 bits (51), Expect = 8.2
Identities = 9/30 (30%), Positives = 15/30 (50%)
Frame = +2
Query: 77 CPHVCLATVGSTRMKTGIAFTANHFLTVIL 166
C +C T ST+ G+ + HF ++L
Sbjct: 234 CKEICNKTNSSTKNAYGVVISEQHFRELLL 263
>SPBC21C3.20c |git1||C2 domain protein Git1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1098
Score = 24.6 bits (51), Expect = 8.2
Identities = 8/12 (66%), Positives = 10/12 (83%)
Frame = +3
Query: 447 TRKIYDFLLPNW 482
TR +YD L+PNW
Sbjct: 826 TRDVYDELVPNW 837
>SPAC19A8.12 |dcp2||mRNA decapping complex subunit
Dcp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 741
Score = 24.6 bits (51), Expect = 8.2
Identities = 14/52 (26%), Positives = 24/52 (46%)
Frame = -3
Query: 454 LRVVYFSNTMQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQXRL 299
L V +S + + KTL +EA++ + +K Q K +PP + L
Sbjct: 618 LEVKNYSKSTDLLKKTLRIPRNDEPLEAANQFDLLKVSPQQKSEVPPKRNEL 669
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,020,381
Number of Sequences: 5004
Number of extensions: 40757
Number of successful extensions: 117
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 109
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 117
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 192109570
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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