BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= rbmnc19c03
(782 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Sch... 111 8e-26
SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha... 81 2e-16
SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosacch... 66 4e-12
SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2... 59 6e-10
SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz... 51 2e-07
SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosac... 47 4e-06
SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosac... 44 3e-05
SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosac... 40 4e-04
SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizo... 40 4e-04
SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac... 38 0.001
SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosacch... 36 0.005
SPCC162.08c |nup211||nuclear pore complex associated protein|Sch... 26 5.3
SPAC222.07c |hri2||eIF2 alpha kinase Hri2|Schizosaccharomyces po... 25 9.3
>SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme
Ubc13|Schizosaccharomyces pombe|chr 1|||Manual
Length = 148
Score = 111 bits (268), Expect = 8e-26
Identities = 49/70 (70%), Positives = 59/70 (84%)
Frame = -2
Query: 781 IDRLGRIXLDIXKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAELWKVNESEAIRN 602
+D+LGRI L K WSPALQIRTVLLSIQAL+ APNPDDPL NDVA++WK NE +AI N
Sbjct: 79 VDKLGRICLSTLKKDWSPALQIRTVLLSIQALMGAPNPDDPLDNDVAKIWKENEPQAIAN 138
Query: 601 AKEWTRRYAM 572
A+EWT++YA+
Sbjct: 139 AREWTKKYAV 148
>SPBC119.02 |ubc4||ubiquitin conjugating enzyme
Ubc4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 147
Score = 80.6 bits (190), Expect = 2e-16
Identities = 37/70 (52%), Positives = 51/70 (72%)
Frame = -2
Query: 781 IDRLGRIXLDIXKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAELWKVNESEAIRN 602
I+ G I LDI +D+WSPAL I VLLSI +LL+ PNPDDPL ++A ++K + S +
Sbjct: 78 INSNGSICLDILRDQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHVYKTDRSRYELS 137
Query: 601 AKEWTRRYAM 572
A+EWTR+YA+
Sbjct: 138 AREWTRKYAI 147
>SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme
Ubc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 217
Score = 66.5 bits (155), Expect = 4e-12
Identities = 28/65 (43%), Positives = 42/65 (64%)
Frame = -2
Query: 769 GRIXLDIXKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAELWKVNESEAIRNAKEW 590
G I LDI KD+WSP +++ L+S+Q+LL P P +P VA+++ N + +R A+EW
Sbjct: 86 GAICLDILKDQWSPVYTMKSALISLQSLLCTPEPSNPQDAQVAQVYLQNYQQFVRTAREW 145
Query: 589 TRRYA 575
T YA
Sbjct: 146 TSSYA 150
>SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2-C
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 176
Score = 59.3 bits (137), Expect = 6e-10
Identities = 27/56 (48%), Positives = 34/56 (60%)
Frame = -2
Query: 781 IDRLGRIXLDIXKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAELWKVNESE 614
+D G I LDI KDKWS ++T+LLS+Q+LL PN PL AELW + E
Sbjct: 106 VDMSGNICLDILKDKWSAVYNVQTILLSLQSLLGEPNNASPLNAQAAELWSKDPIE 161
>SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme
E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 151
Score = 50.8 bits (116), Expect = 2e-07
Identities = 21/59 (35%), Positives = 36/59 (61%)
Frame = -2
Query: 769 GRIXLDIXKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAELWKVNESEAIRNAKE 593
G + LDI +++WSP + +L SIQ+LL+ PN P + A+L + N+ E +R ++
Sbjct: 85 GELCLDILQNRWSPTYDVAAILTSIQSLLNDPNNASPANAEAAQLHRENKKEYVRRVRK 143
>SPAC1250.03 |ubc14||ubiquitin conjugating enzyme
Ubc14|Schizosaccharomyces pombe|chr 1|||Manual
Length = 155
Score = 46.8 bits (106), Expect = 4e-06
Identities = 23/69 (33%), Positives = 41/69 (59%), Gaps = 1/69 (1%)
Frame = -2
Query: 778 DRLGRIXLDIXKDK-WSPALQIRTVLLSIQALLSAPNPDDPLANDVAELWKVNESEAIRN 602
D G + L I K + + P++++R+VL I LL PNPDDPL +AE ++ + +
Sbjct: 85 DSEGNVCLAILKQQVFKPSIKLRSVLEQILQLLREPNPDDPLVASIAEQYRNDRPSFDKI 144
Query: 601 AKEWTRRYA 575
A+++ ++A
Sbjct: 145 ARDYVEQFA 153
>SPCC777.10c |ubc12||ubiquitin conjugating enzyme
Ubc12|Schizosaccharomyces pombe|chr 3|||Manual
Length = 177
Score = 43.6 bits (98), Expect = 3e-05
Identities = 17/47 (36%), Positives = 29/47 (61%)
Frame = -2
Query: 781 IDRLGRIXLDIXKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVA 641
ID G + L+I + W+P L + ++L+ +Q L +PN +DPL + A
Sbjct: 98 IDIEGNVCLNILRQDWNPVLNLNSILVGLQFLFLSPNAEDPLNKEAA 144
>SPBC1198.09 |ubc16||ubiquitin conjugating enzyme
Ubc16|Schizosaccharomyces pombe|chr 2|||Manual
Length = 160
Score = 39.9 bits (89), Expect = 4e-04
Identities = 19/53 (35%), Positives = 30/53 (56%)
Frame = -2
Query: 769 GRIXLDIXKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAELWKVNESEA 611
G + +DI K WSPA +++ L+I +LLS + PL D A+L + + A
Sbjct: 87 GEVCMDILKTHWSPAWSLQSACLAIISLLSNYDASSPLNVDAAKLLRTGDKTA 139
>SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme
Ubc3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 166
Score = 39.9 bits (89), Expect = 4e-04
Identities = 14/54 (25%), Positives = 29/54 (53%)
Frame = -2
Query: 742 DKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAELWKVNESEAIRNAKEWTRR 581
++WSP + +LLS+ ++L+ PN + D ++W+ + E R + R+
Sbjct: 109 ERWSPVQSVEKILLSVMSMLAEPNDESGANIDACKMWREDREEYCRVVRRLARK 162
>SPBC1105.09 |ubc15||ubiquitin conjugating enzyme
Ubc15|Schizosaccharomyces pombe|chr 2|||Manual
Length = 167
Score = 38.3 bits (85), Expect = 0.001
Identities = 16/54 (29%), Positives = 30/54 (55%)
Frame = -2
Query: 742 DKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAELWKVNESEAIRNAKEWTRR 581
++W P T+L+S+ ++LS+PN + P D A+ ++ N E + + RR
Sbjct: 109 ERWLPVHSPETILISVISMLSSPNDESPANIDAAKEFRENPQEFKKRVRRLVRR 162
>SPBC211.07c |ubc8||ubiquitin conjugating enzyme
Ubc8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 36.3 bits (80), Expect = 0.005
Identities = 21/71 (29%), Positives = 32/71 (45%), Gaps = 2/71 (2%)
Frame = -2
Query: 781 IDRL-GRIXLDIXKDKWSPALQIRTVL-LSIQALLSAPNPDDPLANDVAELWKVNESEAI 608
ID L G + LD+ WSP + + + + LL PN DPL + A L +
Sbjct: 77 IDELSGSVCLDVINQTWSPMFDMINIFEVFLPQLLRYPNASDPLNGEAAALLLREPNTYY 136
Query: 607 RNAKEWTRRYA 575
+++ RYA
Sbjct: 137 AKVRDYIARYA 147
>SPCC162.08c |nup211||nuclear pore complex associated
protein|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1837
Score = 26.2 bits (55), Expect = 5.3
Identities = 16/55 (29%), Positives = 28/55 (50%)
Frame = -2
Query: 757 LDIXKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAELWKVNESEAIRNAKE 593
LDI K K P LQ+ + L+S + + ND + +VN + +RN+++
Sbjct: 18 LDIPKTKIDPLLQVDAFTSILIPLISKSKDYESIKND-RIVTEVNYEQQLRNSEK 71
>SPAC222.07c |hri2||eIF2 alpha kinase Hri2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 639
Score = 25.4 bits (53), Expect = 9.3
Identities = 14/39 (35%), Positives = 20/39 (51%)
Frame = +3
Query: 381 WITAKGPAPRPSSTNNKNLIHNSLSQVTLFRMCNITCTH 497
W+TAK P RPS N +QV++ + NI T+
Sbjct: 566 WMTAKDPTKRPSLLEVLNCGLLLPNQVSMPNISNIVSTN 604
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,953,575
Number of Sequences: 5004
Number of extensions: 59945
Number of successful extensions: 143
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 139
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 143
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 379359666
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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