Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= rbmnc19b22
(810 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1223.07c |||aspartate-tRNA ligase |Schizosaccharomyces pombe... 241 6e-65
SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyce... 86 6e-18
SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|ch... 69 8e-13
SPBC1198.10c |||asparagine-tRNA ligase Slm5|Schizosaccharomyces ... 64 2e-11
SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr... 50 5e-07
SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr 3|... 36 0.009
SPAC20H4.01 ||SPAC631.03|U3 snoRNP-associated protein Utp5|Schiz... 28 1.8
SPAC22E12.03c |||THIJ/PFPI family peptidase |Schizosaccharomyces... 28 1.8
SPCC1183.11 ||SPCC31H12.01|MS ion channel protein 1|Schizosaccha... 27 4.2
SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pomb... 26 7.3
SPCC970.07c |raf2|dos2, cmc2, clr7|Rik1-associated factor Raf2|S... 26 7.3
SPAPB8E5.07c |||ribosome biogenesis protein Rrp12|Schizosaccharo... 26 7.3
SPBC216.07c |tor2|SPBC646.01c|phosphatidylinositol kinase Tor2|S... 26 7.3
SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr 3|||Ma... 25 9.6
>SPCC1223.07c |||aspartate-tRNA ligase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 580
Score = 241 bits (591), Expect = 6e-65
Identities = 112/199 (56%), Positives = 149/199 (74%), Gaps = 1/199 (0%)
Frame = -1
Query: 798 EKSDEPEALKIRVNQDTRLDNRVLDLRTPANQAVFRIEAGVCRLFRDILTKKGFVEIHTP 619
E + E ++ +RVN DTRLDNRVLDLRTP NQA+F I+AG+C+ FR+ L F EIHTP
Sbjct: 220 ENAAEGDSKFVRVNLDTRLDNRVLDLRTPTNQAIFDIQAGICQAFREFLLSNSFNEIHTP 279
Query: 618 KIISAASEGGANVFTVSYFKSSAYLAQSPQLYKQMAIAADFDKVFTVGAVFRAEDSNTHR 439
K+ A+SEGG+NVF + YFK+ +L+QSPQLYKQM IAAD ++VF +G VFRAEDSNT+R
Sbjct: 280 KMSGASSEGGSNVFKIQYFKTDGFLSQSPQLYKQMLIAADRERVFEIGPVFRAEDSNTYR 339
Query: 438 HLTEFVGLDLEMSFKQHYHEVLDTIGGTFTEMFRGLRDQFASEIATVGQQYRVEPFKFLD 259
H+TEF GLDLEM+F +HYHEV++ I F +F+ +R+++A ++A V QQY E F D
Sbjct: 340 HMTEFTGLDLEMAFNEHYHEVMEFIEKLFLYIFKTIREKYAKQVAVVRQQYPSEDFILPD 399
Query: 258 PP-LKLEFPQAVQMLKEAG 205
++ F AV++LKEAG
Sbjct: 400 ADRIRFHFKDAVKLLKEAG 418
>SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 568
Score = 85.8 bits (203), Expect = 6e-18
Identities = 61/195 (31%), Positives = 94/195 (48%), Gaps = 5/195 (2%)
Frame = -1
Query: 780 EALKIRVNQDTR----LDNRVLDLRTPANQAVFRIEAGVCRLFRDILTKKGFVEIHTPKI 613
EA R+N + LD R L +R +V ++ A R RD E+ P +
Sbjct: 233 EAFTNRLNAEAEPSYLLDQRHLVIRGETASSVLKVRARALRAMRDTFENLKMTEVTPPCM 292
Query: 612 ISAASEGGANVFTVSYFKSSAYLAQSPQLYKQMAIAADFDKVFTVGAVFRAEDSNTHRHL 433
+ EGGA +F +Y+ AYL QS QLY + A+ A V+T+ FRAE S T RHL
Sbjct: 293 VQTQVEGGATLFKFNYYGQDAYLTQSSQLYLEAALPA-LGSVYTIQESFRAEKSLTRRHL 351
Query: 432 TEFVGLDLEMSFKQHYHEVLDTIGGTFTEMF-RGLRDQFASEIATVGQQYRVEPFKFLDP 256
+EF ++ E+ F ++ E L+ I + R L D A+ + V+P + P
Sbjct: 352 SEFTHVEFELPF-VNFGEFLEIIEEFICQTIDRLLDDPIATPLIKQLNPDFVKPSR---P 407
Query: 255 PLKLEFPQAVQMLKE 211
++L + A++ L E
Sbjct: 408 FMRLSYEDAIKYLNE 422
>SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|chr
3|||Manual
Length = 611
Score = 68.9 bits (161), Expect = 8e-13
Identities = 43/174 (24%), Positives = 72/174 (41%), Gaps = 3/174 (1%)
Frame = -1
Query: 747 RLDNRVLDLRTPANQAVFRIEAGVCRLFRDILTKKGFVEIHTPKIISAASEGGANVFTVS 568
+L NR + LR P + R + + + F E+ TP + + EG S
Sbjct: 142 QLANRHIQLRAPKYNSYLRQRSRLAYQVHSFFNDREFCEVETPLLFKSTPEGAREFVVPS 201
Query: 567 YFKSSAYLA--QSPQLYKQMAIAADFDKVFTVGAVFRAEDSNTHRHLTEFVGLDLEMSFK 394
+ A QSPQ YKQ+ +A+ + + FR ED R EF +DLEMSF
Sbjct: 202 RLNPGKFYALPQSPQQYKQILMASGIGNYYQIARCFRDEDLRFDRQ-PEFTQIDLEMSFV 260
Query: 393 QHYHEVLDTIGGTFTEMFRGLRD-QFASEIATVGQQYRVEPFKFLDPPLKLEFP 235
HE+++ + + + A + Q+ ++ + P ++ E P
Sbjct: 261 DKPHEIMEVVEDLLVRLVSFAKGITLAKPFQHITYQHAIDKYGSDKPDIRFELP 314
>SPBC1198.10c |||asparagine-tRNA ligase Slm5|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 441
Score = 64.5 bits (150), Expect = 2e-11
Identities = 42/128 (32%), Positives = 63/128 (49%), Gaps = 17/128 (13%)
Frame = -1
Query: 729 LDLRTPANQAVFRIEAGVCRLFRDILTKKGFVEIHTPKIISAASEGGANVFTVS------ 568
L LRT +FR+ + + R + K F E + P I S+ EG VFT++
Sbjct: 125 LRLRTAKQGEIFRLRSDSLKALRQFFSSKDFTETNPPIITSSDCEGAGEVFTLTPQETHK 184
Query: 567 -----------YFKSSAYLAQSPQLYKQMAIAADFDKVFTVGAVFRAEDSNTHRHLTEFV 421
+F A+L S QL+ + A+A +V+T+ FRAE S+T RHL EF
Sbjct: 185 NKSFERDDQKHFFDRPAFLTVSTQLHLE-ALALGLSRVYTISPAFRAEQSHTSRHLAEFW 243
Query: 420 GLDLEMSF 397
L+ E++F
Sbjct: 244 MLEAEVAF 251
>SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 591
Score = 49.6 bits (113), Expect = 5e-07
Identities = 31/132 (23%), Positives = 61/132 (46%), Gaps = 3/132 (2%)
Frame = -1
Query: 753 DTRLDNRVLDL-RTPANQAVFRIEAGVCRLFRDILTKKGFVEIHTPKIISAASEGGANVF 577
+ R R LDL + + F + + + + R + F+E+ TP + A A F
Sbjct: 228 EIRHRQRYLDLIMNRSTRDRFVMRSRIIQYIRHFFDSRDFMEVETPMMNMIAGGATAKPF 287
Query: 576 TVSY--FKSSAYLAQSPQLYKQMAIAADFDKVFTVGAVFRAEDSNTHRHLTEFVGLDLEM 403
+ Y+ +P+LY +M + D+V+ +G FR E ++ H EF ++
Sbjct: 288 VTHHNDLDMDLYMRIAPELYLKMLVVGGLDRVYEIGRQFRNEGADL-THNPEFTSIEFYQ 346
Query: 402 SFKQHYHEVLDT 367
++ Y++++DT
Sbjct: 347 AYAD-YYDLMDT 357
>SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr
3|||Manual
Length = 531
Score = 35.5 bits (78), Expect = 0.009
Identities = 26/118 (22%), Positives = 49/118 (41%), Gaps = 2/118 (1%)
Frame = -1
Query: 759 NQDTRLDNRVLDLRTPANQA-VFRIEAGVCRLFRDILTKKGFVEIHTPKIISAASEGGAN 583
N + R + R +D+ + + + R +++GF+E+ TP + A
Sbjct: 182 NYEKRFEKRFVDMMSNTKSLELLEKRYRIIESIRKFFSERGFLEVETPILSHHFGGATAR 241
Query: 582 VFTVS-YFKSSAYLAQSPQLYKQMAIAADFDKVFTVGAVFRAEDSNTHRHLTEFVGLD 412
F S K L +P+L+ + + ++VF +G FR E + H EF +
Sbjct: 242 PFITSDIHKLPLTLRCAPELWLKQLVIGGMNRVFELGKNFRNEGIDA-THNPEFTSCE 298
>SPAC20H4.01 ||SPAC631.03|U3 snoRNP-associated protein
Utp5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 666
Score = 27.9 bits (59), Expect = 1.8
Identities = 20/70 (28%), Positives = 37/70 (52%), Gaps = 1/70 (1%)
Frame = +2
Query: 599 DAADIIFGVWI-STKPFFVRISLKSLQTPASILKTAWLAGVRRSKTRLSSRVSWFTRIFK 775
++ D + VW ST+ F + + L + A T L + RSKTRL+S+ + ++
Sbjct: 343 ESTDSLTVVWKESTRTVFETVPWRILSSQA----TNGLIELVRSKTRLTSKNKTVSNVYD 398
Query: 776 ASGSSLFSGL 805
S +++ SG+
Sbjct: 399 ESNATISSGV 408
>SPAC22E12.03c |||THIJ/PFPI family peptidase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 191
Score = 27.9 bits (59), Expect = 1.8
Identities = 23/95 (24%), Positives = 40/95 (42%)
Frame = -1
Query: 525 YKQMAIAADFDKVFTVGAVFRAEDSNTHRHLTEFVGLDLEMSFKQHYHEVLDTIGGTFTE 346
YK++ A DF K + + + T FV ++ +K+ + GT T
Sbjct: 58 YKEIPSADDFAKQYDIAIIPGGGLGAKTLSTTPFVQQVVKEFYKKPNKWIGMICAGTLTA 117
Query: 345 MFRGLRDQFASEIATVGQQYRVEPFKFLDPPLKLE 241
GL ++ + +V Q +K+LD P+ LE
Sbjct: 118 KTSGLPNKQITGHPSVRGQLEEGGYKYLDQPVVLE 152
>SPCC1183.11 ||SPCC31H12.01|MS ion channel protein
1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1011
Score = 26.6 bits (56), Expect = 4.2
Identities = 10/21 (47%), Positives = 14/21 (66%)
Frame = +2
Query: 665 KSLQTPASILKTAWLAGVRRS 727
K +Q P S+L T W+ +RRS
Sbjct: 693 KVIQAPNSLLNTLWILNMRRS 713
>SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 2685
Score = 25.8 bits (54), Expect = 7.3
Identities = 21/83 (25%), Positives = 39/83 (46%), Gaps = 5/83 (6%)
Frame = -1
Query: 666 FRDILTKKGFVEIHTPKII-SAASEGGANVFTVS----YFKSSAYLAQSPQLYKQMAIAA 502
F D+ T + P+++ S S ++FT+ +K A ++ +L +++ +AA
Sbjct: 2102 FEDLDTHADTLTFDFPRVVFSTDSSQYYDIFTIITDLLLYKEPAQKQRNQRL-EEIMLAA 2160
Query: 501 DFDKVFTVGAVFRAEDSNTHRHL 433
DF + V RA + HR L
Sbjct: 2161 DFSDLTGTSEVVRALQARVHRLL 2183
>SPCC970.07c |raf2|dos2, cmc2, clr7|Rik1-associated factor
Raf2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 636
Score = 25.8 bits (54), Expect = 7.3
Identities = 16/41 (39%), Positives = 20/41 (48%)
Frame = +2
Query: 623 VWISTKPFFVRISLKSLQTPASILKTAWLAGVRRSKTRLSS 745
VW S PFF+RI I K + A R K++LSS
Sbjct: 180 VW-SESPFFIRIRSSYEHLILQINKNIYNARQERKKSKLSS 219
>SPAPB8E5.07c |||ribosome biogenesis protein Rrp12|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1163
Score = 25.8 bits (54), Expect = 7.3
Identities = 10/16 (62%), Positives = 13/16 (81%)
Frame = +1
Query: 631 LDEAFLREDIPEEPAD 678
+DEAF++ED EEP D
Sbjct: 1022 MDEAFVQEDNDEEPLD 1037
>SPBC216.07c |tor2|SPBC646.01c|phosphatidylinositol kinase
Tor2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2337
Score = 25.8 bits (54), Expect = 7.3
Identities = 15/39 (38%), Positives = 20/39 (51%), Gaps = 1/39 (2%)
Frame = +1
Query: 652 EDIPEE-PADPGLDPEDGLVSRRAQIQNPIVEPGVLVHA 765
E I EE P +D + L+S Q+Q P G+L HA
Sbjct: 1249 EFIEEELVTKPSVDTIEALISINNQLQQPDAAIGILKHA 1287
>SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 630
Score = 25.4 bits (53), Expect = 9.6
Identities = 18/75 (24%), Positives = 35/75 (46%)
Frame = +2
Query: 569 ETVNTLAPPSDAADIIFGVWISTKPFFVRISLKSLQTPASILKTAWLAGVRRSKTRLSSR 748
E L PS +W ++K FF++++ +S Q PAS + ++ +L +
Sbjct: 544 EFTKQLGLPSPTHISTAAIWNTSKEFFIQLA-ESAQYPASSIIRIPEEDSNKTNYQLVAA 602
Query: 749 VSWFTRIFKASGSSL 793
++ F + + G SL
Sbjct: 603 LTAFGALLLSVGGSL 617
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,156,813
Number of Sequences: 5004
Number of extensions: 64420
Number of successful extensions: 185
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 178
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 181
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 394431430
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -