BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= rbmnc18j22
(706 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAP27G11.15 |slx1||structure-specific endonuclease catalytic su... 29 0.65
SPAC23A1.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 27 2.6
SPAC30C2.02 |mmd1||deoxyhypusine hydroxylase |Schizosaccharomyce... 26 4.6
SPAC1834.08 |mak1|phk3|histidine kinase Mak1|Schizosaccharomyces... 26 6.0
SPAC16A10.03c |||zinc finger protein Pep5/Vps11 |Schizosaccharom... 26 6.0
SPAC12G12.01c ||SPAC630.02|ubiquitin-protein ligase E3|Schizosac... 25 8.0
>SPAP27G11.15 |slx1||structure-specific endonuclease catalytic
subunit |Schizosaccharomyces pombe|chr 1|||Manual
Length = 271
Score = 29.1 bits (62), Expect = 0.65
Identities = 14/60 (23%), Positives = 31/60 (51%), Gaps = 2/60 (3%)
Frame = -3
Query: 401 ECNV--KAVDSACMRCRRSFAVYPAVTYLHCGHSCLCTDCDETVNVDNTCPKCKSGIRYK 228
+CN+ + ++S +R F ++ +L C S T+ + + ++ C KCK +R++
Sbjct: 179 KCNLCYECIESDELRANCPFTDCNSINHLTCLASSFLTEECQVLPIEGMCTKCKRVLRWR 238
>SPAC23A1.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 251
Score = 27.1 bits (57), Expect = 2.6
Identities = 21/71 (29%), Positives = 31/71 (43%), Gaps = 5/71 (7%)
Frame = -3
Query: 407 NTECNVKAVDSACMRCRRSFAVYPAVTYLH---CGHS-CLCTDCDETVNVDN-TCPKCKS 243
+ E V CM C + A+T + CGH+ C V+N +CP CK
Sbjct: 180 SAETTVPQECGLCMMCVQRGDERVAITTPYTTDCGHTYCYACIMSRLKLVNNVSCPICKH 239
Query: 242 GIRYKLKYKTL 210
IR+ L +T+
Sbjct: 240 RIRFALPDQTM 250
>SPAC30C2.02 |mmd1||deoxyhypusine hydroxylase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 318
Score = 26.2 bits (55), Expect = 4.6
Identities = 14/48 (29%), Positives = 27/48 (56%)
Frame = -1
Query: 157 AQILNLNLPLAYQKSVLFASNSAKVNERIRRLARDNNERATTPIFTHK 14
++I++ NLPL Y+ V+F + E + LA + + +P+F H+
Sbjct: 183 SEIVDQNLPLFYRYRVMFRLRNIGNEEAV--LALTDGFKDPSPLFRHE 228
>SPAC1834.08 |mak1|phk3|histidine kinase Mak1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1639
Score = 25.8 bits (54), Expect = 6.0
Identities = 11/34 (32%), Positives = 21/34 (61%)
Frame = +3
Query: 225 QFISNAAFTFRTRIVHVYRFVAIGAQTRMSAMQI 326
Q++SN + RT ++ + V+ +T+MSA Q+
Sbjct: 996 QYLSNMSHEIRTPLIGITGMVSFLLETQMSAEQL 1029
>SPAC16A10.03c |||zinc finger protein Pep5/Vps11
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 860
Score = 25.8 bits (54), Expect = 6.0
Identities = 19/68 (27%), Positives = 29/68 (42%)
Frame = -3
Query: 443 LEIIKTDRNLPLNTECNVKAVDSACMRCRRSFAVYPAVTYLHCGHSCLCTDCDETVNVDN 264
+++IK D ++ + C C V P V+Y C H + DC +
Sbjct: 769 IQVIKNDIEKKRQLLGTIQDSEKVCDNCEGLLDV-PFVSY-SCLH-LVHRDCA----TET 821
Query: 263 TCPKCKSG 240
CPKCK+G
Sbjct: 822 VCPKCKAG 829
>SPAC12G12.01c ||SPAC630.02|ubiquitin-protein ligase
E3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 905
Score = 25.4 bits (53), Expect = 8.0
Identities = 15/41 (36%), Positives = 20/41 (48%)
Frame = -1
Query: 142 LNLPLAYQKSVLFASNSAKVNERIRRLARDNNERATTPIFT 20
++ L V A S + ++I RLARD ER IFT
Sbjct: 479 MDTELTSMSDVFEAKESKFLEKKILRLARDVIERLDLDIFT 519
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,796,130
Number of Sequences: 5004
Number of extensions: 54984
Number of successful extensions: 162
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 157
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 162
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 327172622
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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