Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= rbmnc17n19
(825 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 327 1e-90
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 327 1e-90
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 327 1e-90
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 102 7e-23
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 58 1e-09
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 57 3e-09
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 4.3
SPBC21D10.06c |map4||cell agglutination protein Map4|Schizosacch... 26 5.6
SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein... 26 7.5
SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificit... 25 9.9
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 25 9.9
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 327 bits (803), Expect = 1e-90
Identities = 149/190 (78%), Positives = 168/190 (88%)
Frame = -1
Query: 825 DVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFN 646
DVYKIGGIGTVPVGRVETGV+KPG IV FAPA +TTEVKSVEMHHE+L +PGDNVGFN
Sbjct: 250 DVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFN 309
Query: 645 VKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACK 466
VKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACK
Sbjct: 310 VKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACK 369
Query: 465 FAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQ 286
FAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQ
Sbjct: 370 FAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQ 429
Query: 285 TVAVGVIKAV 256
TVAVGVIKAV
Sbjct: 430 TVAVGVIKAV 439
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 327 bits (803), Expect = 1e-90
Identities = 149/190 (78%), Positives = 168/190 (88%)
Frame = -1
Query: 825 DVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFN 646
DVYKIGGIGTVPVGRVETGV+KPG IV FAPA +TTEVKSVEMHHE+L +PGDNVGFN
Sbjct: 250 DVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFN 309
Query: 645 VKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACK 466
VKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACK
Sbjct: 310 VKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACK 369
Query: 465 FAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQ 286
FAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQ
Sbjct: 370 FAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQ 429
Query: 285 TVAVGVIKAV 256
TVAVGVIKAV
Sbjct: 430 TVAVGVIKAV 439
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 327 bits (803), Expect = 1e-90
Identities = 149/190 (78%), Positives = 168/190 (88%)
Frame = -1
Query: 825 DVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFN 646
DVYKIGGIGTVPVGRVETGV+KPG IV FAPA +TTEVKSVEMHHE+L +PGDNVGFN
Sbjct: 250 DVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFN 309
Query: 645 VKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACK 466
VKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACK
Sbjct: 310 VKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACK 369
Query: 465 FAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQ 286
FAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQ
Sbjct: 370 FAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQ 429
Query: 285 TVAVGVIKAV 256
TVAVGVIKAV
Sbjct: 430 TVAVGVIKAV 439
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 102 bits (244), Expect = 7e-23
Identities = 60/190 (31%), Positives = 101/190 (53%), Gaps = 2/190 (1%)
Frame = -1
Query: 816 KIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSV-EMHHEALQEAVPGDNVGFNVK 640
K +GT+ G++E G +K + V+ P N T EV ++ + E + ++ GD V V+
Sbjct: 476 KYKDLGTILEGKIEAGSIKKNSNVLVMPINQTLEVTAIYDEADEEISSSICGDQVRLRVR 535
Query: 639 NVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFA 460
+++ GYV +KN P F AQ+ +L P ++ GY+ V+ HTA FA
Sbjct: 536 GDD-SDVQTGYVLTSTKN-PVHATTRFIAQIAILELPSILTTGYSCVMHIHTAVEEVSFA 593
Query: 459 EIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTV 280
++ K+D+ T + ++ P G I L P+C+E F+++ +GRF +RD TV
Sbjct: 594 KLLHKLDK-TNRKSKKPPMFATKGMKIIAELETQTPVCMERFEDYQYMGRFTLRDQGTTV 652
Query: 279 AVG-VIKAVN 253
AVG V+K ++
Sbjct: 653 AVGKVVKILD 662
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 58.4 bits (135), Expect = 1e-09
Identities = 49/189 (25%), Positives = 83/189 (43%), Gaps = 2/189 (1%)
Frame = -1
Query: 825 DVYKIGGIGTVPVGRVETGVLKPGTIV--VFAPANITTEVKSVEMHHEALQEAVPGDNVG 652
DV+ I G GTV GRVE G LK G + V +++ T V +EM + L AV GDN G
Sbjct: 259 DVFSISGRGTVVTGRVERGTLKKGAEIEIVGYGSHLKTTVTGIEMFKKQLDAAVAGDNCG 318
Query: 651 FNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIA 472
++++ ++L+RG + P F A +L + T +D + +
Sbjct: 319 LLLRSIKREQLKRGMIVAQPGTVAPH--QKFKASFYILTK--EEGGRRTGFVDKYRPQLY 374
Query: 471 CKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDM 292
+ +++ ++ T + K + GD + P+ +E Q RF VR+
Sbjct: 375 SRTSDVTVEL---THPDPNDSDKMVMPGDNVEMICTLIHPIVIEKGQ------RFTVREG 425
Query: 291 RQTVAVGVI 265
TV ++
Sbjct: 426 GSTVGTALV 434
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 56.8 bits (131), Expect = 3e-09
Identities = 54/189 (28%), Positives = 85/189 (44%), Gaps = 1/189 (0%)
Frame = -1
Query: 825 DVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQE-AVPGDNVGF 649
DVY+ TV GRVE G ++ ++ + VK+V + + AV GD V
Sbjct: 411 DVYRSPRSVTV-TGRVEAGNVQVNQVLYDVSSQEDAYVKNVIRNSDPSSTWAVAGDTVTL 469
Query: 648 NVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIAC 469
+ ++ V +LR G + + +N P + F A++ + G I +G T VL H+
Sbjct: 470 QLADIEVNQLRPGDILSNYEN-PVRRVRSFVAEIQTFDIHGPILSGSTLVL-----HLGR 523
Query: 468 KFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMR 289
+ K+ K + + S K I L PLC+ +E P LGRF +R
Sbjct: 524 TVTSVSLKIVTVNNKRSR-HIASRKRALVRISFLDGLFPLCLA--EECPALGRFILRRSG 580
Query: 288 QTVAVGVIK 262
TVA G++K
Sbjct: 581 DTVAAGIVK 589
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 26.6 bits (56), Expect = 4.3
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = +1
Query: 619 QFLDGHVLYVETYIVSRYSFLESFV 693
+F DGH+L ETY+ LES V
Sbjct: 527 KFFDGHLLASETYVGGHVESLESGV 551
>SPBC21D10.06c |map4||cell agglutination protein
Map4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 948
Score = 26.2 bits (55), Expect = 5.6
Identities = 13/32 (40%), Positives = 20/32 (62%)
Frame = +2
Query: 713 TSVVMLAGAKTTMVPGFNTPVSTLPTGTVPIP 808
+SVV+ + +T V + + VST TGTV +P
Sbjct: 86 SSVVLYSAKETVTVSSYWSLVSTSVTGTVYVP 117
>SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 670
Score = 25.8 bits (54), Expect = 7.5
Identities = 24/85 (28%), Positives = 42/85 (49%), Gaps = 2/85 (2%)
Frame = +2
Query: 212 SAALVTLPPPASLKLTALMTPTATVCLMSRTAKRPRGGNSWKDSTH--RGLEGTKLTMAA 385
S +VTLPPPAS ++ T T T + S ++ G+ + +++ + ++++
Sbjct: 183 STDIVTLPPPAS-STSSFSTITNTSMIPSSSSFTTTTGSPYYNTSSFLPSSVISSASLSS 241
Query: 386 SPDLMDFGLTSVDLPVRRSTFSLIS 460
S L +TS PV S+ SL S
Sbjct: 242 SSVLPTSIITSTSTPVTVSSSSLSS 266
>SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificity
factor complex subunit Rna14|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 733
Score = 25.4 bits (53), Expect = 9.9
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = +2
Query: 764 NTPVSTLPTGTVPIPPIL 817
N P S LPT VP+P I+
Sbjct: 666 NPPTSALPTVPVPLPSII 683
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 25.4 bits (53), Expect = 9.9
Identities = 11/25 (44%), Positives = 18/25 (72%)
Frame = +2
Query: 377 MAASPDLMDFGLTSVDLPVRRSTFS 451
++ +PDL D L+SVD P++ +T S
Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNTCS 51
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,514,387
Number of Sequences: 5004
Number of extensions: 74567
Number of successful extensions: 231
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 221
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 228
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 404442380
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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