Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= rbmnc17m22
(792 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 336 3e-93
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 169 5e-43
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 153 3e-38
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 122 8e-29
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 95 1e-20
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 89 5e-19
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 79 5e-16
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 70 3e-13
SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 44 2e-05
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 28 1.8
SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc... 26 5.4
SPAC977.16c |dak2||dihydroxyacetone kinase Dak2 |Schizosaccharom... 26 7.1
SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccha... 26 7.1
SPBC3D6.11c |slx8||ubiquitin-protein ligase E3 Slx8 |Schizosacch... 26 7.1
SPAC1142.03c |swi2|SPAC17G6.20c|Swi5 complex subunit Swi2|Schizo... 25 9.4
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 336 bits (825), Expect = 3e-93
Identities = 157/177 (88%), Positives = 166/177 (93%)
Frame = -2
Query: 791 SFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCP 612
+F+TTAEREIVRDIKEKLCYVALDFEQE+ TAA SSSLEKSYELPDGQVITIGNERFR P
Sbjct: 199 TFSTTAEREIVRDIKEKLCYVALDFEQELQTAAQSSSLEKSYELPDGQVITIGNERFRAP 258
Query: 611 EALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEIT 432
EALFQPS LG+E GIHE TYNSIMKCDVDIRKDLY N V+SGGTTMYPGIADRMQKEI
Sbjct: 259 EALFQPSALGLENAGIHEATYNSIMKCDVDIRKDLYGNVVMSGGTTMYPGIADRMQKEIQ 318
Query: 431 ALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 261
ALAPS+MK+KI+APPERKYSVWIGGSILASLSTFQQMWISKQEYDESGP IV+RKCF
Sbjct: 319 ALAPSSMKVKIVAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPGIVYRKCF 375
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 169 bits (410), Expect = 5e-43
Identities = 78/174 (44%), Positives = 116/174 (66%)
Frame = -2
Query: 782 TTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEAL 603
++AE+EIVR+IKEK CYVA DF E+ + +Y+LPD Q IT+G E F PE L
Sbjct: 207 SSAEKEIVREIKEKCCYVASDFRSEIESWTEHKPQIHTYQLPDNQTITLGTECFSAPEVL 266
Query: 602 FQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALA 423
F P +G EA G+H + SI+ D+D+R LY+N VLSGG+T+ G +R E+ A++
Sbjct: 267 FNPEMMGSEASGLHIQLFKSILLSDIDLRSTLYSNIVLSGGSTLLRGFGERFISELRAIS 326
Query: 422 PSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 261
++KI A PER ++ W+GGSILASLSTF+++ I+ +EY ++ +++ R+ F
Sbjct: 327 GKKNQVKIYASPERMHNAWLGGSILASLSTFRRLLITSEEY-KNDQNVIFRRRF 379
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 153 bits (371), Expect = 3e-38
Identities = 78/180 (43%), Positives = 117/180 (65%), Gaps = 12/180 (6%)
Frame = -2
Query: 791 SFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCP 612
+F TA+ E VR++KEKLCYV+ D E + + ++ L ++Y LPDG+VI +G+ER+ CP
Sbjct: 199 AFNRTADFETVREMKEKLCYVSYDLELDHKLSEETTVLMRNYTLPDGRVIKVGSERYECP 258
Query: 611 EALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEIT 432
E LFQP +G E G+ E +++I DVDIRK LY VLSGG++MY G+ R++KEI
Sbjct: 259 ECLFQPHLVGSEQPGLSEFIFDTIQAADVDIRKYLYRAIVLSGGSSMYAGLPSRLEKEIK 318
Query: 431 AL--------APSTM---KIKIIAPPERKYSVWIGGSILAS-LSTFQQMWISKQEYDESG 288
L P+ + K+KI P R+++V+IGG++LA ++ MW+SK E++E G
Sbjct: 319 QLWFERVLHGDPARLPNFKVKIEDAPRRRHAVFIGGAVLADIMAQNDHMWVSKAEWEEYG 378
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 122 bits (293), Expect = 8e-29
Identities = 70/179 (39%), Positives = 99/179 (55%), Gaps = 11/179 (6%)
Frame = -2
Query: 791 SFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCP 612
S+ +R ++ + KE + V LD + A A+S + K +E PDG G ERFR
Sbjct: 243 SYEIFQKRRVIEEWKESVLDV-LDTPFDEAKASSRNP--KPFEFPDGVTHKFGQERFRIS 299
Query: 611 EALFQPSFLGMEAC---------GIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGI 459
E LF PSF + G+HE Y SI+ CD ++R L N V++GGT++ PG+
Sbjct: 300 EILFNPSFSASRSAETTPPQGSVGLHELVYQSILACDSELRSPLLNNIVVTGGTSLIPGL 359
Query: 458 ADRMQKEITALAP-STMKIKII-APPERKYSVWIGGSILASLSTFQQMWISKQEYDESG 288
++R+Q E+ LA S + + +VW GGSILASL FQ +W+SKQEYDE G
Sbjct: 360 SERLQAEVQRLATGSRINVHTAETASATSNAVWFGGSILASLDNFQHLWVSKQEYDEVG 418
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 95.1 bits (226), Expect = 1e-20
Identities = 47/108 (43%), Positives = 71/108 (65%), Gaps = 6/108 (5%)
Frame = -2
Query: 569 GIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAP 390
GI + N I +CDVDIR L N ++ GGT++ G + R+Q E++ L P + ++KI A
Sbjct: 326 GIPQLFQNCISECDVDIRASLLNNVIVCGGTSLMQGFSLRLQNELSKLYPGS-RLKIHAS 384
Query: 389 P---ERKYSVWIGGSILASLSTFQQMWISKQEYDESGP---SIVHRKC 264
ER Y+ W+GGSIL+SL TF Q+WIS+QEY+E G +++ ++C
Sbjct: 385 GHVVERSYASWLGGSILSSLGTFHQLWISRQEYEEHGSDRLALIEKRC 432
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 89.4 bits (212), Expect = 5e-19
Identities = 60/189 (31%), Positives = 89/189 (47%), Gaps = 20/189 (10%)
Frame = -2
Query: 773 EREIVRDIKEKLCYVALDFEQEMATAASS--SSLEKSYELPD------------------ 654
E IV +IKE +CYV+ +F+++M S LE Y LPD
Sbjct: 211 ETYIVNEIKESVCYVSQNFKEDMEICHEKPRSKLEICYALPDYSTGKHGYIVRDINQKIE 270
Query: 653 GQVITIGNERFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTT 474
QV+ + NERF PE LF PS + + GI E S+ +I+ L N V GG
Sbjct: 271 QQVLNLSNERFMIPELLFSPSDIEIREAGIPEAVMQSVTHFPENIQAMLLENVVTIGGNC 330
Query: 473 MYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDE 294
+PG R+ E+ +LAP+ ++K+ P + W S + L + I++ EY E
Sbjct: 331 KFPGFHKRLSSELRSLAPANWEVKVFEPSDPICFPWKKASHM-PLEHWNANKITRSEYSE 389
Query: 293 SGPSIVHRK 267
G +I+ RK
Sbjct: 390 HGANIMTRK 398
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 79.4 bits (187), Expect = 5e-16
Identities = 53/180 (29%), Positives = 83/180 (46%), Gaps = 19/180 (10%)
Frame = -2
Query: 752 IKEKLCYVALDFEQEMATAASSSS--LEKSYELPDGQVITI--GNERFRCPEALFQPSFL 585
IKE+ CYV D +E + L+ + E G TI G ERF PE F P
Sbjct: 240 IKEECCYVCPDIVKEFSRFDREPDRYLKYASESITGHSTTIDVGFERFLAPEIFFNPEIA 299
Query: 584 GME-ACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPST-- 414
+ + E N + +D+RK LY N VLSGG+T++ +R+Q+++ +
Sbjct: 300 SSDFLTPLPELVDNVVQSSPIDVRKGLYKNIVLSGGSTLFKNFGNRLQRDLKRIVDERIH 359
Query: 413 ------------MKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHR 270
+ + +I+ ++ +VW GGS+LA F +K +Y+E G SI R
Sbjct: 360 RSEMLSGAKSGGVDVNVISHKRQRNAVWFGGSLLAQTPEFGSYCHTKADYEEYGASIARR 419
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 70.1 bits (164), Expect = 3e-13
Identities = 31/118 (26%), Positives = 62/118 (52%)
Frame = -2
Query: 629 ERFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADR 450
E+ R PE +F PS +G++ GI E + + + ++ ++ L +N +++GG PG+ R
Sbjct: 594 EQIRVPEVIFSPSIVGIDQAGILEIMRSILQRHSLEEQQKLVSNVLITGGLGSLPGMETR 653
Query: 449 MQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIV 276
+++E+T++ P I + W G S + F+ ++++EY E GP +
Sbjct: 654 IKRELTSIMPVGSSINVFRASNPLLDAWKGASEWSVTEKFKAAKVTREEYLEKGPEYI 711
>SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 44.4 bits (100), Expect = 2e-05
Identities = 20/86 (23%), Positives = 42/86 (48%), Gaps = 3/86 (3%)
Frame = -2
Query: 515 KDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPP---ERKYSVWIGGSILA 345
K+LY++ ++ GG +PG A +++ I + + I +I PP + ++ W G I
Sbjct: 527 KNLYSSILIVGGAGQFPGFAHLLEERIHSKRANIPTISVIPPPRSMDAQFVAWKGACIYN 586
Query: 344 SLSTFQQMWISKQEYDESGPSIVHRK 267
+ ++WI ++ G ++ K
Sbjct: 587 RIRIVSELWIKNSDWKMLGSRVLQYK 612
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 27.9 bits (59), Expect = 1.8
Identities = 12/37 (32%), Positives = 24/37 (64%), Gaps = 3/37 (8%)
Frame = -2
Query: 368 WIGGSILASLSTFQQM---WISKQEYDESGPSIVHRK 267
++GGSI+A S + + +++ +EY + GP+ +H K
Sbjct: 486 FLGGSIVAKTSFNESVSSHYVTLEEYAQHGPTAIHTK 522
>SPAC29B12.07 |sec16||multidomain vesicle coat component
Sec16|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1995
Score = 26.2 bits (55), Expect = 5.4
Identities = 14/51 (27%), Positives = 19/51 (37%)
Frame = -3
Query: 790 RSLPPPSGKSFVXXXXXXXXXXXXXXXRWPLLHPAAPSRSLTNFPTVRSSP 638
RSLPPPSG+ PL P + S ++P + P
Sbjct: 643 RSLPPPSGQVNAPMSQTPNPISFAYQHGTPLATPTMRANSFNSYPASSAEP 693
>SPAC977.16c |dak2||dihydroxyacetone kinase Dak2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 591
Score = 25.8 bits (54), Expect = 7.1
Identities = 15/42 (35%), Positives = 23/42 (54%)
Frame = +3
Query: 630 VSDGDDLTVGKFVRLLEGAAGCSSGHLLLEVESDVAELLLDV 755
V+ GDD++VGK L G G + L+ ++ A L LD+
Sbjct: 135 VAVGDDVSVGKKRGALVGRRGLGATVLVHKIAGSAAALGLDL 176
>SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 617
Score = 25.8 bits (54), Expect = 7.1
Identities = 13/37 (35%), Positives = 20/37 (54%)
Frame = -2
Query: 443 KEITALAPSTMKIKIIAPPERKYSVWIGGSILASLST 333
KEI++ A S + + + P E+ W ILA L+T
Sbjct: 212 KEISSQARSNIALPTLRPQEKNLIDWRDRKILAPLTT 248
>SPBC3D6.11c |slx8||ubiquitin-protein ligase E3 Slx8
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 269
Score = 25.8 bits (54), Expect = 7.1
Identities = 12/37 (32%), Positives = 19/37 (51%), Gaps = 2/37 (5%)
Frame = -3
Query: 694 HPAAPSRSLTNFPTVRSSPSETKDSV--AQRLSSNPR 590
HPA+ SLTN ++ + P+ D + S +PR
Sbjct: 131 HPASVENSLTNVNSIHAQPTTISDMIDLTDETSYDPR 167
>SPAC1142.03c |swi2|SPAC17G6.20c|Swi5 complex subunit
Swi2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 722
Score = 25.4 bits (53), Expect = 9.4
Identities = 27/121 (22%), Positives = 50/121 (41%), Gaps = 4/121 (3%)
Frame = -2
Query: 722 DFEQEMATAASSSSLEKSY--ELPDGQ-VITIGNERFRCPEALFQPSFLG-MEACGIHET 555
D E++ SS+ + +Y + + Q ++T+ NE + E L S + E+
Sbjct: 108 DDHPEISGHVSSNDKDFAYFEDKSENQPLVTLPNENNQVIEPLSSQSCKSQLSTQNYSES 167
Query: 554 TYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKY 375
+ CD+D ++ + + T ++P AD I ++K A RKY
Sbjct: 168 DFGWNQLCDLD---PIFKSLAFTDDTNLFPAFADSEAALIMLKKREMTRVKHRAGRPRKY 224
Query: 374 S 372
S
Sbjct: 225 S 225
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,127,282
Number of Sequences: 5004
Number of extensions: 63606
Number of successful extensions: 226
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 207
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 218
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 385381248
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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