Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= rbmnc17m11
         (912 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC4F10.03c |||2'-O-ribose methyltransferase|Schizosaccharomyce...    56   9e-09
SPAC1687.11 |spb1||rRNA methyltransferase Spb1 |Schizosaccharomy...    44   2e-05
SPBC2G2.15c |||mitochondrial 2' O-ribose methyltransferase Mrm2 ...    38   0.001
SPAC607.03c |snu13||U3 snoRNP-associated protein Snu13|Schizosac...    29   1.2  
SPAC17G6.03 |||phosphoprotein phosphatase|Schizosaccharomyces po...    28   2.1  
SPBC29A3.09c |||AAA family ATPase Gcn20 |Schizosaccharomyces pom...    27   3.7  
SPBC776.06c |||spindle pole body interacting protein |Schizosacc...    27   3.7  
SPCC4B3.03c |||DUF21 domain protein|Schizosaccharomyces pombe|ch...    26   6.5  
SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ...    26   6.5  
SPAC11E3.02c |||C2 domain protein|Schizosaccharomyces pombe|chr ...    26   8.5  
SPBC106.19 ||SPBC582.01|sequence orphan|Schizosaccharomyces pomb...    26   8.5  

>SPAC4F10.03c |||2'-O-ribose methyltransferase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 285

 Score = 55.6 bits (128), Expect = 9e-09
 Identities = 48/191 (25%), Positives = 85/191 (44%), Gaps = 4/191 (2%)
 Frame = -3

Query: 628 RPRCWRKLLEIDKKFHVCRHVDTFLDLCGGPGEFANYTMSLNPLCKAYGVTLTNNSVCVY 449
           R R   KLL+++++F++       +DLC  PG ++        L K    ++  +     
Sbjct: 22  RARSAFKLLQLNEQFNLFEGAKRVVDLCAAPGSWSQVLS--RELLKNIDTSIAADE---- 75

Query: 448 KPTVCK--RKNFTTITGP-DKSGDVFDKNVVFEISIKCGNA-CDLVLADGSVDVNGRENE 281
           KP +     +    I G      D+   N +  I    GN   DLV++DG+ DV G  + 
Sbjct: 76  KPMIVAVDLQPMAPIDGVCTLQLDITHPNTLSIILSHFGNEPADLVVSDGAPDVTGLHDL 135

Query: 280 QERLNFDLIMCETQLILICLRPGGNCVLKVFDAFEHKTIQMLNKFVNHFEKWVLYKPPSS 101
            E +   +++    L +  L+PGG  V K+F   +   +    + +  F K    KP SS
Sbjct: 136 DEYIQAQILLAAFNLAVCVLKPGGKFVAKIFRGRDVSLLYSQLRLM--FRKVSCAKPRSS 193

Query: 100 RPANSERYLIC 68
           R ++ E +++C
Sbjct: 194 RASSIESFVVC 204


>SPAC1687.11 |spb1||rRNA methyltransferase Spb1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 802

 Score = 44.4 bits (100), Expect = 2e-05
 Identities = 47/187 (25%), Positives = 78/187 (41%)
 Frame = -3

Query: 628 RPRCWRKLLEIDKKFHVCRHVDTFLDLCGGPGEFANYTMSLNPLCKAYGVTLTNNSVCVY 449
           R R   KL+++++K+         +DLC  PG    +    +  CK  G  +    +   
Sbjct: 26  RSRAAFKLVQLNQKYSFLEKAKVIIDLCAAPG---GWLQVASKTCKP-GSLIVGVDLAPI 81

Query: 448 KPTVCKRKNFTTITGPDKSGDVFDKNVVFEISIKCGNACDLVLADGSVDVNGRENEQERL 269
           KP      N  T    D + D     +     +K   A D+VL DG+ +V     +    
Sbjct: 82  KPI----PNCHTFV-EDITSDKCRSQL--RGYLKTWKA-DVVLHDGAPNVGSAWLQDAYG 133

Query: 268 NFDLIMCETQLILICLRPGGNCVLKVFDAFEHKTIQMLNKFVNHFEKWVLYKPPSSRPAN 89
              L++   +L    L  GG  V KVF + ++  +  +  F   F K    KPPSSR  +
Sbjct: 134 QAQLVLMSMKLACEFLVAGGTFVTKVFRSRDYNNLLWV--FKQLFNKVEATKPPSSRNVS 191

Query: 88  SERYLIC 68
           +E +++C
Sbjct: 192 AEIFVVC 198


>SPBC2G2.15c |||mitochondrial 2' O-ribose methyltransferase Mrm2
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 218

 Score = 38.3 bits (85), Expect = 0.001
 Identities = 50/223 (22%), Positives = 90/223 (40%), Gaps = 13/223 (5%)
 Frame = -3

Query: 688 FSSKSV---VCARSKLFDKRPTR----RPRCWRKLLEIDKKFHVCRHVDTFLDLCGGPGE 530
           F SK++      RSK F ++ ++    R R   KL+E++ K+      D  +D+   PG 
Sbjct: 2   FGSKTLFSWAAKRSKDFYRKKSKIDNFRSRAAYKLIELNSKYRFINKEDVVIDVGFAPGS 61

Query: 529 FANYTMSL-NPLCKAYGVTLTNNSVCVYKPTVCKRKNFTTITGPDKSGDVFDK-NVVFEI 356
           ++     L     K  G+ + + +     P       +  I  P+    +F+   ++ E 
Sbjct: 62  WSQVAKKLVGNKGKVIGIDIQHIA-----PPEGVLPIYGDIRDPNTLTKLFEALRLLHEP 116

Query: 355 ----SIKCGNACDLVLADGSVDVNGRENEQERLNFDLIMCETQLILICLRPGGNCVLKVF 188
               SI C    D V++D      G       L+ +L      + L  L+  G+ + K +
Sbjct: 117 NTNDSIDC-RVVDAVISDMLHKATGIRIRDHALSMELCASALHVALTFLKSNGSFICKFY 175

Query: 187 DAFEHKTIQMLNKFVNHFEKWVLYKPPSSRPANSERYLICFNK 59
              E   +Q L K  +HF    + KP +S   + E Y +C  +
Sbjct: 176 MGDEDADLQNLLK--SHFRFVQVMKPKASLKESREAYFVCLER 216


>SPAC607.03c |snu13||U3 snoRNP-associated protein
           Snu13|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 125

 Score = 28.7 bits (61), Expect = 1.2
 Identities = 13/34 (38%), Positives = 18/34 (52%)
 Frame = +3

Query: 258 KSKLRRSCSFSRPLTSTEPSANTKSHALPHLMLI 359
           K+ L R+C  SRP+ S   + N  S  LP +  I
Sbjct: 83  KAALGRACGVSRPVISASITTNEASDLLPQIQAI 116


>SPAC17G6.03 |||phosphoprotein phosphatase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 635

 Score = 27.9 bits (59), Expect = 2.1
 Identities = 15/36 (41%), Positives = 18/36 (50%)
 Frame = -1

Query: 192 FLTRSNTKRFKC*TSLLTILKNGFYTNRLLLGLPIP 85
           F+  S TK+    TSL   + NG Y  R  LGL  P
Sbjct: 359 FMFHSKTKKSSFNTSLGEFISNGIYEARKALGLLTP 394


>SPBC29A3.09c |||AAA family ATPase Gcn20 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 736

 Score = 27.1 bits (57), Expect = 3.7
 Identities = 10/28 (35%), Positives = 17/28 (60%)
 Frame = -3

Query: 634 TRRPRCWRKLLEIDKKFHVCRHVDTFLD 551
           TR  RC  +L E +K+    +H+ +F+D
Sbjct: 442 TREERCKNQLREYEKQMEYRKHLQSFID 469


>SPBC776.06c |||spindle pole body interacting protein
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 598

 Score = 27.1 bits (57), Expect = 3.7
 Identities = 14/38 (36%), Positives = 18/38 (47%), Gaps = 1/38 (2%)
 Frame = +2

Query: 350 DANLEYNIFIKHVTRFVGPRNGCEIFAFAHC-RFVHAH 460
           D+N+ Y +      R  G R G  +FA A C  F H H
Sbjct: 80  DSNMYYVLNTIRAKRVEGTRRGGSVFAMAICTTFPHVH 117


>SPCC4B3.03c |||DUF21 domain protein|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 679

 Score = 26.2 bits (55), Expect = 6.5
 Identities = 11/25 (44%), Positives = 16/25 (64%)
 Frame = +3

Query: 495 QSGFNDMV*LANSPGPPHRSKNVST 569
           Q G + M+ ++NSPG PH +  V T
Sbjct: 347 QEGKSHMILISNSPGEPHGAIGVIT 371


>SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1044

 Score = 26.2 bits (55), Expect = 6.5
 Identities = 19/69 (27%), Positives = 31/69 (44%), Gaps = 1/69 (1%)
 Frame = -3

Query: 322  LADGSVDVNGRENEQERLNFDLI-MCETQLILICLRPGGNCVLKVFDAFEHKTIQMLNKF 146
            LA+ +  +N R+ E ERL  D+I +    L +  L+   N   +     E K  ++  K 
Sbjct: 942  LAEANEKLNARDEEIERLKVDIIGLQNASLNMQSLKDSDN---RTISDLESKNKELEKKL 998

Query: 145  VNHFEKWVL 119
                E W+L
Sbjct: 999  KEADEYWLL 1007


>SPAC11E3.02c |||C2 domain protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1237

 Score = 25.8 bits (54), Expect = 8.5
 Identities = 10/19 (52%), Positives = 14/19 (73%)
 Frame = +3

Query: 612 RQHLGLRVGRLSNNFERAQ 668
           R+H+G  VGR  ++ ERAQ
Sbjct: 924 REHIGFYVGRTYHDLERAQ 942


>SPBC106.19 ||SPBC582.01|sequence orphan|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 515

 Score = 25.8 bits (54), Expect = 8.5
 Identities = 9/29 (31%), Positives = 16/29 (55%)
 Frame = +2

Query: 728 QHFYASCRSYGLCRKRRPTRSAKTIQIKN 814
           ++F  SCR + +C      +SA  + +KN
Sbjct: 8   RNFLCSCRGFSVCTPYASIKSASELALKN 36


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,548,782
Number of Sequences: 5004
Number of extensions: 72410
Number of successful extensions: 203
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 193
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 201
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 462505890
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)