Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= rbmnc17h03
(828 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 345 5e-96
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 172 6e-44
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 159 4e-40
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 122 8e-29
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 95 1e-20
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 89 5e-19
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 79 6e-16
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 70 4e-13
SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 44 2e-05
SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc... 29 0.80
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 28 1.9
SPAC977.16c |dak2||dihydroxyacetone kinase Dak2 |Schizosaccharom... 26 7.5
SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccha... 26 7.5
SPBC3D6.11c |slx8||ubiquitin-protein ligase E3 Slx8 |Schizosacch... 26 7.5
SPAC1142.03c |swi2|SPAC17G6.20c|Swi5 complex subunit Swi2|Schizo... 25 9.9
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 345 bits (848), Expect = 5e-96
Identities = 161/181 (88%), Positives = 170/181 (93%)
Frame = -1
Query: 825 ERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNER 646
ERGY+F+TTAEREIVRDIKEKLCYVALDFEQE+ TAA SSSLEKSYELPDGQVITIGNER
Sbjct: 195 ERGYTFSTTAEREIVRDIKEKLCYVALDFEQELQTAAQSSSLEKSYELPDGQVITIGNER 254
Query: 645 FRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQ 466
FR PEALFQPS LG+E GIHE TYNSIMKCDVDIRKDLY N V+SGGTTMYPGIADRMQ
Sbjct: 255 FRAPEALFQPSALGLENAGIHEATYNSIMKCDVDIRKDLYGNVVMSGGTTMYPGIADRMQ 314
Query: 465 KEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKC 286
KEI ALAPS+MK+KI+APPERKYSVWIGGSILASLSTFQQMWISKQEYDESGP IV+RKC
Sbjct: 315 KEIQALAPSSMKVKIVAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPGIVYRKC 374
Query: 285 F 283
F
Sbjct: 375 F 375
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 172 bits (418), Expect = 6e-44
Identities = 79/179 (44%), Positives = 118/179 (65%)
Frame = -1
Query: 819 GYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFR 640
G+ ++AE+EIVR+IKEK CYVA DF E+ + +Y+LPD Q IT+G E F
Sbjct: 202 GHELVSSAEKEIVREIKEKCCYVASDFRSEIESWTEHKPQIHTYQLPDNQTITLGTECFS 261
Query: 639 CPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKE 460
PE LF P +G EA G+H + SI+ D+D+R LY+N VLSGG+T+ G +R E
Sbjct: 262 APEVLFNPEMMGSEASGLHIQLFKSILLSDIDLRSTLYSNIVLSGGSTLLRGFGERFISE 321
Query: 459 ITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 283
+ A++ ++KI A PER ++ W+GGSILASLSTF+++ I+ +EY ++ +++ R+ F
Sbjct: 322 LRAISGKKNQVKIYASPERMHNAWLGGSILASLSTFRRLLITSEEY-KNDQNVIFRRRF 379
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 159 bits (386), Expect = 4e-40
Identities = 80/183 (43%), Positives = 120/183 (65%), Gaps = 12/183 (6%)
Frame = -1
Query: 822 RGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERF 643
+GY+F TA+ E VR++KEKLCYV+ D E + + ++ L ++Y LPDG+VI +G+ER+
Sbjct: 196 KGYAFNRTADFETVREMKEKLCYVSYDLELDHKLSEETTVLMRNYTLPDGRVIKVGSERY 255
Query: 642 RCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQK 463
CPE LFQP +G E G+ E +++I DVDIRK LY VLSGG++MY G+ R++K
Sbjct: 256 ECPECLFQPHLVGSEQPGLSEFIFDTIQAADVDIRKYLYRAIVLSGGSSMYAGLPSRLEK 315
Query: 462 EITAL--------APSTM---KIKIIAPPERKYSVWIGGSILAS-LSTFQQMWISKQEYD 319
EI L P+ + K+KI P R+++V+IGG++LA ++ MW+SK E++
Sbjct: 316 EIKQLWFERVLHGDPARLPNFKVKIEDAPRRRHAVFIGGAVLADIMAQNDHMWVSKAEWE 375
Query: 318 ESG 310
E G
Sbjct: 376 EYG 378
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 122 bits (293), Expect = 8e-29
Identities = 70/179 (39%), Positives = 99/179 (55%), Gaps = 11/179 (6%)
Frame = -1
Query: 813 SFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCP 634
S+ +R ++ + KE + V LD + A A+S + K +E PDG G ERFR
Sbjct: 243 SYEIFQKRRVIEEWKESVLDV-LDTPFDEAKASSRNP--KPFEFPDGVTHKFGQERFRIS 299
Query: 633 EALFQPSFLGMEAC---------GIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGI 481
E LF PSF + G+HE Y SI+ CD ++R L N V++GGT++ PG+
Sbjct: 300 EILFNPSFSASRSAETTPPQGSVGLHELVYQSILACDSELRSPLLNNIVVTGGTSLIPGL 359
Query: 480 ADRMQKEITALAP-STMKIKII-APPERKYSVWIGGSILASLSTFQQMWISKQEYDESG 310
++R+Q E+ LA S + + +VW GGSILASL FQ +W+SKQEYDE G
Sbjct: 360 SERLQAEVQRLATGSRINVHTAETASATSNAVWFGGSILASLDNFQHLWVSKQEYDEVG 418
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 95.1 bits (226), Expect = 1e-20
Identities = 47/108 (43%), Positives = 71/108 (65%), Gaps = 6/108 (5%)
Frame = -1
Query: 591 GIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAP 412
GI + N I +CDVDIR L N ++ GGT++ G + R+Q E++ L P + ++KI A
Sbjct: 326 GIPQLFQNCISECDVDIRASLLNNVIVCGGTSLMQGFSLRLQNELSKLYPGS-RLKIHAS 384
Query: 411 P---ERKYSVWIGGSILASLSTFQQMWISKQEYDESGP---SIVHRKC 286
ER Y+ W+GGSIL+SL TF Q+WIS+QEY+E G +++ ++C
Sbjct: 385 GHVVERSYASWLGGSILSSLGTFHQLWISRQEYEEHGSDRLALIEKRC 432
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 89.4 bits (212), Expect = 5e-19
Identities = 60/189 (31%), Positives = 89/189 (47%), Gaps = 20/189 (10%)
Frame = -1
Query: 795 EREIVRDIKEKLCYVALDFEQEMATAASS--SSLEKSYELPD------------------ 676
E IV +IKE +CYV+ +F+++M S LE Y LPD
Sbjct: 211 ETYIVNEIKESVCYVSQNFKEDMEICHEKPRSKLEICYALPDYSTGKHGYIVRDINQKIE 270
Query: 675 GQVITIGNERFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTT 496
QV+ + NERF PE LF PS + + GI E S+ +I+ L N V GG
Sbjct: 271 QQVLNLSNERFMIPELLFSPSDIEIREAGIPEAVMQSVTHFPENIQAMLLENVVTIGGNC 330
Query: 495 MYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDE 316
+PG R+ E+ +LAP+ ++K+ P + W S + L + I++ EY E
Sbjct: 331 KFPGFHKRLSSELRSLAPANWEVKVFEPSDPICFPWKKASHM-PLEHWNANKITRSEYSE 389
Query: 315 SGPSIVHRK 289
G +I+ RK
Sbjct: 390 HGANIMTRK 398
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 79.4 bits (187), Expect = 6e-16
Identities = 53/180 (29%), Positives = 83/180 (46%), Gaps = 19/180 (10%)
Frame = -1
Query: 774 IKEKLCYVALDFEQEMATAASSSS--LEKSYELPDGQVITI--GNERFRCPEALFQPSFL 607
IKE+ CYV D +E + L+ + E G TI G ERF PE F P
Sbjct: 240 IKEECCYVCPDIVKEFSRFDREPDRYLKYASESITGHSTTIDVGFERFLAPEIFFNPEIA 299
Query: 606 GME-ACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPST-- 436
+ + E N + +D+RK LY N VLSGG+T++ +R+Q+++ +
Sbjct: 300 SSDFLTPLPELVDNVVQSSPIDVRKGLYKNIVLSGGSTLFKNFGNRLQRDLKRIVDERIH 359
Query: 435 ------------MKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHR 292
+ + +I+ ++ +VW GGS+LA F +K +Y+E G SI R
Sbjct: 360 RSEMLSGAKSGGVDVNVISHKRQRNAVWFGGSLLAQTPEFGSYCHTKADYEEYGASIARR 419
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 70.1 bits (164), Expect = 4e-13
Identities = 31/118 (26%), Positives = 62/118 (52%)
Frame = -1
Query: 651 ERFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADR 472
E+ R PE +F PS +G++ GI E + + + ++ ++ L +N +++GG PG+ R
Sbjct: 594 EQIRVPEVIFSPSIVGIDQAGILEIMRSILQRHSLEEQQKLVSNVLITGGLGSLPGMETR 653
Query: 471 MQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIV 298
+++E+T++ P I + W G S + F+ ++++EY E GP +
Sbjct: 654 IKRELTSIMPVGSSINVFRASNPLLDAWKGASEWSVTEKFKAAKVTREEYLEKGPEYI 711
>SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 44.4 bits (100), Expect = 2e-05
Identities = 20/86 (23%), Positives = 42/86 (48%), Gaps = 3/86 (3%)
Frame = -1
Query: 537 KDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPP---ERKYSVWIGGSILA 367
K+LY++ ++ GG +PG A +++ I + + I +I PP + ++ W G I
Sbjct: 527 KNLYSSILIVGGAGQFPGFAHLLEERIHSKRANIPTISVIPPPRSMDAQFVAWKGACIYN 586
Query: 366 SLSTFQQMWISKQEYDESGPSIVHRK 289
+ ++WI ++ G ++ K
Sbjct: 587 RIRIVSELWIKNSDWKMLGSRVLQYK 612
>SPAC29B12.07 |sec16||multidomain vesicle coat component
Sec16|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1995
Score = 29.1 bits (62), Expect = 0.80
Identities = 16/56 (28%), Positives = 21/56 (37%)
Frame = -2
Query: 827 PSAATRSLPPPSGKSFVXXXXXXXXXXXXXXXRWPLLHPAAPSRSLTNFPTVRSSP 660
PS RSLPPPSG+ PL P + S ++P + P
Sbjct: 638 PSNPPRSLPPPSGQVNAPMSQTPNPISFAYQHGTPLATPTMRANSFNSYPASSAEP 693
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 27.9 bits (59), Expect = 1.9
Identities = 12/37 (32%), Positives = 24/37 (64%), Gaps = 3/37 (8%)
Frame = -1
Query: 390 WIGGSILASLSTFQQM---WISKQEYDESGPSIVHRK 289
++GGSI+A S + + +++ +EY + GP+ +H K
Sbjct: 486 FLGGSIVAKTSFNESVSSHYVTLEEYAQHGPTAIHTK 522
>SPAC977.16c |dak2||dihydroxyacetone kinase Dak2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 591
Score = 25.8 bits (54), Expect = 7.5
Identities = 15/42 (35%), Positives = 23/42 (54%)
Frame = +1
Query: 652 VSDGDDLTVGKFVRLLEGAAGCSSGHLLLEVESDVAELLLDV 777
V+ GDD++VGK L G G + L+ ++ A L LD+
Sbjct: 135 VAVGDDVSVGKKRGALVGRRGLGATVLVHKIAGSAAALGLDL 176
>SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 617
Score = 25.8 bits (54), Expect = 7.5
Identities = 13/37 (35%), Positives = 20/37 (54%)
Frame = -1
Query: 465 KEITALAPSTMKIKIIAPPERKYSVWIGGSILASLST 355
KEI++ A S + + + P E+ W ILA L+T
Sbjct: 212 KEISSQARSNIALPTLRPQEKNLIDWRDRKILAPLTT 248
>SPBC3D6.11c |slx8||ubiquitin-protein ligase E3 Slx8
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 269
Score = 25.8 bits (54), Expect = 7.5
Identities = 12/37 (32%), Positives = 19/37 (51%), Gaps = 2/37 (5%)
Frame = -2
Query: 716 HPAAPSRSLTNFPTVRSSPSETKDSV--AQRLSSNPR 612
HPA+ SLTN ++ + P+ D + S +PR
Sbjct: 131 HPASVENSLTNVNSIHAQPTTISDMIDLTDETSYDPR 167
>SPAC1142.03c |swi2|SPAC17G6.20c|Swi5 complex subunit
Swi2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 722
Score = 25.4 bits (53), Expect = 9.9
Identities = 27/121 (22%), Positives = 50/121 (41%), Gaps = 4/121 (3%)
Frame = -1
Query: 744 DFEQEMATAASSSSLEKSY--ELPDGQ-VITIGNERFRCPEALFQPSFLG-MEACGIHET 577
D E++ SS+ + +Y + + Q ++T+ NE + E L S + E+
Sbjct: 108 DDHPEISGHVSSNDKDFAYFEDKSENQPLVTLPNENNQVIEPLSSQSCKSQLSTQNYSES 167
Query: 576 TYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKY 397
+ CD+D ++ + + T ++P AD I ++K A RKY
Sbjct: 168 DFGWNQLCDLD---PIFKSLAFTDDTNLFPAFADSEAALIMLKKREMTRVKHRAGRPRKY 224
Query: 396 S 394
S
Sbjct: 225 S 225
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,210,745
Number of Sequences: 5004
Number of extensions: 65301
Number of successful extensions: 232
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 213
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 224
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 406444570
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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