Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= rbmnc17b18
(844 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyce... 309 4e-85
SPBC1198.10c |||asparagine-tRNA ligase Slm5|Schizosaccharomyces ... 112 7e-26
SPCC1223.07c |||aspartate-tRNA ligase |Schizosaccharomyces pombe... 66 4e-12
SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr... 36 0.005
SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|ch... 32 0.089
SPAC9.03c |brr2|spp41|U5 snRNP complex subunit Brr2 |Schizosacch... 29 0.82
SPAC1039.05c |||conserved fungal protein|Schizosaccharomyces pom... 27 2.5
SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyce... 27 2.5
SPBP8B7.07c |set6||histone lysine methyltransferase Set6 |Schizo... 27 3.3
>SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 568
Score = 309 bits (758), Expect = 4e-85
Identities = 149/269 (55%), Positives = 182/269 (67%), Gaps = 1/269 (0%)
Frame = -1
Query: 844 GSTLFKFSYFGEEAYLTQSFQLYLETCLATXGDVYCIAQSYRAEQSRTRRHLAEYSHVEA 665
G+TLFKF+Y+G++AYLTQS QLYLE L G VY I +S+RAE+S TRRHL+E++HVE
Sbjct: 300 GATLFKFNYYGQDAYLTQSSQLYLEAALPALGSVYTIQESFRAEKSLTRRHLSEFTHVEF 359
Query: 664 ECPFITFXXXXXXXXXXXXXXXXXXLASPEGQ-LVYELXXXXXXXXXXXKRMTYIEAIEY 488
E PF+ F L P L+ +L R++Y +AI+Y
Sbjct: 360 ELPFVNFGEFLEIIEEFICQTIDRLLDDPIATPLIKQLNPDFVKPSRPFMRLSYEDAIKY 419
Query: 487 LRANNITKEDGSFYEFGEDIPEMPERKMTDAIGVPILLCKFPAEIKSFYMSRCVDDRRLT 308
L +NI +G ++FG+DI E ERKMTD I PI L FP EIKSFYM R VD LT
Sbjct: 420 LNEHNILTPEGEQHKFGDDIAEAAERKMTDQINRPIFLTYFPLEIKSFYMKRVVDRPELT 479
Query: 307 ESVDVLMPGVGEIVGGSMRIWDHEELMEGYKREGIDPSPYYWYTDQRKFGSVPHGGYGLG 128
ESVD LMP VGEIVGGSMRI D +EL+ YKREGIDP+PYYW+T+QRK+G+ HGG GLG
Sbjct: 480 ESVDCLMPNVGEIVGGSMRISDIQELLAAYKREGIDPAPYYWFTEQRKYGTTEHGGCGLG 539
Query: 127 LERFLCWLLDRYHIRDVCLYPRFLERCTP 41
LERFL WL DRY +R+ CL+PRF ERCTP
Sbjct: 540 LERFLAWLCDRYTVRECCLFPRFTERCTP 568
>SPBC1198.10c |||asparagine-tRNA ligase Slm5|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 441
Score = 112 bits (269), Expect = 7e-26
Identities = 83/255 (32%), Positives = 123/255 (48%), Gaps = 2/255 (0%)
Frame = -1
Query: 820 YFGEEAYLTQSFQLYLETCLATXGDVYCIAQSYRAEQSRTRRHLAEYSHVEAECPFITFX 641
+F A+LT S QL+LE VY I+ ++RAEQS T RHLAE+ +EAE F+T
Sbjct: 196 FFDRPAFLTVSTQLHLEALALGLSRVYTISPAFRAEQSHTSRHLAEFWMLEAEVAFMTSL 255
Query: 640 XXXXXXXXXXXXXXXXXLASPEGQLVYELXXXXXXXXXXXKRMTYIEAIEYLRANNITKE 461
L Y MTY EAIE L A T +
Sbjct: 256 SQLTSLMEDMIKYTLNSLMEQN----YHRDHWDQLLKPWKC-MTYSEAIEELSAVKKTWK 310
Query: 460 DGSFYEFGEDIPEMPERKMTDAIG-VPILLCKFPAEIKSFYMSRCVDDRRLTESVDVLMP 284
++G D+ E+ + + + P+ + +P +IK FYM D +VD+L+P
Sbjct: 311 YPP--KWGNDLSSEHEKYLCEILHKTPVFVTDYPQKIKPFYMKSSGPDT--VAAVDLLVP 366
Query: 283 GVGEIVGGSMRIWDHEELMEGYKREGIDPSPYYWYTDQRKFGSVPHGGYGLGLERFLCWL 104
VGE+ GGS+R DH ++ YK P WY D K+ + PHGG+GLG+ER + +L
Sbjct: 367 QVGELAGGSLRK-DH---LDEYKTY---PPELQWYLDLMKYSNAPHGGFGLGIERLIAFL 419
Query: 103 L-DRYHIRDVCLYPR 62
+ ++++ +PR
Sbjct: 420 EGENTNVKETIPFPR 434
>SPCC1223.07c |||aspartate-tRNA ligase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 580
Score = 66.5 bits (155), Expect = 4e-12
Identities = 40/111 (36%), Positives = 57/111 (51%), Gaps = 1/111 (0%)
Frame = -1
Query: 370 KFPAEIKSFYMSRCVDDRRLTESVDVLMPGVGEIVGGSMRIWDHEELMEGYKREGIDPSP 191
K+P+ ++ FY +D R + S D M G EI+ G+ RI D E L+E K G+ P
Sbjct: 471 KYPSSVRPFYTMPDPEDPRYSNSYDFFMKGQ-EIMSGAQRIHDPELLVERMKALGVSPDV 529
Query: 190 -YYWYTDQRKFGSVPHGGYGLGLERFLCWLLDRYHIRDVCLYPRFLERCTP 41
Y D G PH G G+GLER + + L+ +IR +PR +R P
Sbjct: 530 GLQQYIDAFAIGCPPHAGGGIGLERVVMFYLNLPNIRLASSFPRDPKRLLP 580
Score = 50.8 bits (116), Expect = 2e-07
Identities = 25/65 (38%), Positives = 39/65 (60%), Gaps = 1/65 (1%)
Frame = -1
Query: 844 GSTLFKFSYFGEEAYLTQSFQLYLETCLATXGD-VYCIAQSYRAEQSRTRRHLAEYSHVE 668
GS +FK YF + +L+QS QLY + +A + V+ I +RAE S T RH+ E++ ++
Sbjct: 289 GSNVFKIQYFKTDGFLSQSPQLYKQMLIAADRERVFEIGPVFRAEDSNTYRHMTEFTGLD 348
Query: 667 AECPF 653
E F
Sbjct: 349 LEMAF 353
>SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 591
Score = 36.3 bits (80), Expect = 0.005
Identities = 14/34 (41%), Positives = 24/34 (70%)
Frame = -1
Query: 166 KFGSVPHGGYGLGLERFLCWLLDRYHIRDVCLYP 65
++G P GG+G+G++R + +L D IR+V L+P
Sbjct: 542 EYGLPPTGGWGMGVDRLVMFLTDSNTIREVLLFP 575
>SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|chr
3|||Manual
Length = 611
Score = 32.3 bits (70), Expect = 0.089
Identities = 24/85 (28%), Positives = 40/85 (47%), Gaps = 6/85 (7%)
Frame = -1
Query: 298 DVLMPGVGEIVGGSMRIWDHEELMEGYKREGIDPSPYYWYTDQRKF------GSVPHGGY 137
D+++ G+ E+ GGS+RI H ++ + + + P Y G PHGG
Sbjct: 495 DIVVNGI-ELGGGSIRI--HNPDIQRFVLKDVLKLPENRYATFEHLIRVLSSGCPPHGGI 551
Query: 136 GLGLERFLCWLLDRYHIRDVCLYPR 62
LG +R L + IR+V +P+
Sbjct: 552 ALGFDRLAALLTNAPGIREVIAFPK 576
>SPAC9.03c |brr2|spp41|U5 snRNP complex subunit Brr2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2176
Score = 29.1 bits (62), Expect = 0.82
Identities = 11/30 (36%), Positives = 14/30 (46%)
Frame = +1
Query: 367 TCTAVSARQWRPSSSAPAFPGCPLQTRRRI 456
TC + R W S FP CP + RR+
Sbjct: 1152 TCKMIEKRLWPTMSPLRQFPNCPSEVIRRV 1181
>SPAC1039.05c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 781
Score = 27.5 bits (58), Expect = 2.5
Identities = 9/22 (40%), Positives = 12/22 (54%)
Frame = -1
Query: 163 FGSVPHGGYGLGLERFLCWLLD 98
FG+ P Y + F+CWL D
Sbjct: 201 FGTSPFNAYQQNTDNFVCWLFD 222
>SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1067
Score = 27.5 bits (58), Expect = 2.5
Identities = 16/54 (29%), Positives = 29/54 (53%), Gaps = 2/54 (3%)
Frame = -1
Query: 307 ESVDVLMPGVGEIVGGSMRIWDHEELMEGYKREGIDPSPYYWYTD--QRKFGSV 152
++V + G+ EI+ + D E+L + Y +EG+ P WY D +R F ++
Sbjct: 989 KTVAAKLSGMKEILFPKSNLADWEQLPD-YVKEGLTGVPVAWYDDVFKRVFSNI 1041
>SPBP8B7.07c |set6||histone lysine methyltransferase Set6
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 483
Score = 27.1 bits (57), Expect = 3.3
Identities = 12/26 (46%), Positives = 16/26 (61%), Gaps = 3/26 (11%)
Frame = -1
Query: 163 FGSVPH---GGYGLGLERFLCWLLDR 95
FG + H G Y + L+ F+ WLLDR
Sbjct: 396 FGGLHHCILGRYSISLDDFVLWLLDR 421
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,814,042
Number of Sequences: 5004
Number of extensions: 50824
Number of successful extensions: 148
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 140
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 144
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 416455520
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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