Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= rbmnc16k11
(806 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 310 2e-85
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 310 2e-85
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 310 2e-85
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 101 2e-22
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 54 2e-08
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 50 3e-07
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 4.1
SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein... 26 7.2
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 25 9.6
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 310 bits (761), Expect = 2e-85
Identities = 141/182 (77%), Positives = 160/182 (87%)
Frame = -3
Query: 804 GTVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKE 625
GTVPVGRVETGV+KPG IV FAPA +TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK+
Sbjct: 258 GTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKD 317
Query: 624 LRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKV 445
+RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+
Sbjct: 318 IRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKI 377
Query: 444 DRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIK 265
DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIK
Sbjct: 378 DRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIK 437
Query: 264 AV 259
AV
Sbjct: 438 AV 439
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 310 bits (761), Expect = 2e-85
Identities = 141/182 (77%), Positives = 160/182 (87%)
Frame = -3
Query: 804 GTVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKE 625
GTVPVGRVETGV+KPG IV FAPA +TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK+
Sbjct: 258 GTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKD 317
Query: 624 LRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKV 445
+RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+
Sbjct: 318 IRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKI 377
Query: 444 DRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIK 265
DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIK
Sbjct: 378 DRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIK 437
Query: 264 AV 259
AV
Sbjct: 438 AV 439
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 310 bits (761), Expect = 2e-85
Identities = 141/182 (77%), Positives = 160/182 (87%)
Frame = -3
Query: 804 GTVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKE 625
GTVPVGRVETGV+KPG IV FAPA +TTEVKSVEMHHE+L +PGDNVGFNVKNVSVK+
Sbjct: 258 GTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKD 317
Query: 624 LRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKV 445
+RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+
Sbjct: 318 IRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKI 377
Query: 444 DRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIK 265
DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIK
Sbjct: 378 DRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIK 437
Query: 264 AV 259
AV
Sbjct: 438 AV 439
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 101 bits (241), Expect = 2e-22
Identities = 59/185 (31%), Positives = 99/185 (53%), Gaps = 2/185 (1%)
Frame = -3
Query: 804 GTVPVGRVETGVLKPGTIVVFAPANITTEVKSV-EMHHEALQEAVPGDNVGFNVKNVSVK 628
GT+ G++E G +K + V+ P N T EV ++ + E + ++ GD V V+
Sbjct: 481 GTILEGKIEAGSIKKNSNVLVMPINQTLEVTAIYDEADEEISSSICGDQVRLRVRGDD-S 539
Query: 627 ELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEK 448
+++ GYV +KN P F AQ+ +L P ++ GY+ V+ HTA FA++ K
Sbjct: 540 DVQTGYVLTSTKN-PVHATTRFIAQIAILELPSILTTGYSCVMHIHTAVEEVSFAKLLHK 598
Query: 447 VDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVG-V 271
+D+ T + ++ P G I L P+C+E F+++ +GRF +RD TVAVG V
Sbjct: 599 LDK-TNRKSKKPPMFATKGMKIIAELETQTPVCMERFEDYQYMGRFTLRDQGTTVAVGKV 657
Query: 270 IKAVN 256
+K ++
Sbjct: 658 VKILD 662
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 54.4 bits (125), Expect = 2e-08
Identities = 49/176 (27%), Positives = 79/176 (44%), Gaps = 1/176 (0%)
Frame = -3
Query: 789 GRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQE-AVPGDNVGFNVKNVSVKELRRG 613
GRVE G ++ ++ + VK+V + + AV GD V + ++ V +LR G
Sbjct: 423 GRVEAGNVQVNQVLYDVSSQEDAYVKNVIRNSDPSSTWAVAGDTVTLQLADIEVNQLRPG 482
Query: 612 YVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRT 433
+ + +N P + F A++ + G I +G T VL H+ + K+
Sbjct: 483 DILSNYEN-PVRRVRSFVAEIQTFDIHGPILSGSTLVL-----HLGRTVTSVSLKIVTVN 536
Query: 432 GKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIK 265
K + + S K I L PLC+ +E P LGRF +R TVA G++K
Sbjct: 537 NKRSR-HIASRKRALVRISFLDGLFPLCLA--EECPALGRFILRRSGDTVAAGIVK 589
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 50.4 bits (115), Expect = 3e-07
Identities = 45/181 (24%), Positives = 78/181 (43%), Gaps = 2/181 (1%)
Frame = -3
Query: 804 GTVPVGRVETGVLKPGTIV--VFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSV 631
GTV GRVE G LK G + V +++ T V +EM + L AV GDN G ++++
Sbjct: 267 GTVVTGRVERGTLKKGAEIEIVGYGSHLKTTVTGIEMFKKQLDAAVAGDNCGLLLRSIKR 326
Query: 630 KELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKE 451
++L+RG + P F A +L + T +D + + + +++
Sbjct: 327 EQLKRGMIVAQPGTVAPH--QKFKASFYILTK--EEGGRRTGFVDKYRPQLYSRTSDVTV 382
Query: 450 KVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGV 271
++ T + K + GD + P+ +E Q RF VR+ TV +
Sbjct: 383 EL---THPDPNDSDKMVMPGDNVEMICTLIHPIVIEKGQ------RFTVREGGSTVGTAL 433
Query: 270 I 268
+
Sbjct: 434 V 434
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 26.6 bits (56), Expect = 4.1
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = +1
Query: 622 QFLDGHVLYVETYIVSRYSFLESFV 696
+F DGH+L ETY+ LES V
Sbjct: 527 KFFDGHLLASETYVGGHVESLESGV 551
>SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 670
Score = 25.8 bits (54), Expect = 7.2
Identities = 24/85 (28%), Positives = 42/85 (49%), Gaps = 2/85 (2%)
Frame = +2
Query: 215 SAALVTLPPPASLKLTALMTPTATVCLMSRTAKRPRGGNSWKDSTH--RGLEGTKLTMAA 388
S +VTLPPPAS ++ T T T + S ++ G+ + +++ + ++++
Sbjct: 183 STDIVTLPPPAS-STSSFSTITNTSMIPSSSSFTTTTGSPYYNTSSFLPSSVISSASLSS 241
Query: 389 SPDLMDFGLTSVDLPVRRSTFSLIS 463
S L +TS PV S+ SL S
Sbjct: 242 SSVLPTSIITSTSTPVTVSSSSLSS 266
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 25.4 bits (53), Expect = 9.6
Identities = 11/25 (44%), Positives = 18/25 (72%)
Frame = +2
Query: 380 MAASPDLMDFGLTSVDLPVRRSTFS 454
++ +PDL D L+SVD P++ +T S
Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNTCS 51
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,357,518
Number of Sequences: 5004
Number of extensions: 69807
Number of successful extensions: 206
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 196
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 203
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 392429240
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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