Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= rbmnc16k06
(803 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyce... 228 8e-61
SPBC1198.10c |||asparagine-tRNA ligase Slm5|Schizosaccharomyces ... 84 3e-17
SPCC1223.07c |||aspartate-tRNA ligase |Schizosaccharomyces pombe... 66 4e-12
SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr... 36 0.005
SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|ch... 32 0.083
SPAC9.03c |brr2|spp41|U5 snRNP complex subunit Brr2 |Schizosacch... 29 0.77
SPAC1039.05c |||conserved fungal protein|Schizosaccharomyces pom... 27 2.4
SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyce... 27 2.4
SPBP8B7.07c |set6||histone lysine methyltransferase Set6 |Schizo... 27 3.1
SPBC16G5.14c |rps3||40S ribosomal protein S3|Schizosaccharomyces... 27 4.1
>SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 568
Score = 228 bits (557), Expect = 8e-61
Identities = 103/159 (64%), Positives = 122/159 (76%)
Frame = -2
Query: 523 RMTYIEAIEYLRANNITKEDGSFYEFGEDIPEMPERKMTDAIGVPILLCKFPAEIKSFYM 344
R++Y +AI+YL +NI +G ++FG+DI E ERKMTD I PI L FP EIKSFYM
Sbjct: 410 RLSYEDAIKYLNEHNILTPEGEQHKFGDDIAEAAERKMTDQINRPIFLTYFPLEIKSFYM 469
Query: 343 SRCVDDRRLTESVDVLMPGVGEIVGGSMRIWDHEELMEGYKREGIDPSPYYWYTDQRKFG 164
R VD LTESVD LMP VGEIVGGSMRI D +EL+ YKREGIDP+PYYW+T+QRK+G
Sbjct: 470 KRVVDRPELTESVDCLMPNVGEIVGGSMRISDIQELLAAYKREGIDPAPYYWFTEQRKYG 529
Query: 163 SVPHGGYGLGLERFLCWLLDRYHIRDVCLYPRFLERCTP 47
+ HGG GLGLERFL WL DRY +R+ CL+PRF ERCTP
Sbjct: 530 TTEHGGCGLGLERFLAWLCDRYTVRECCLFPRFTERCTP 568
Score = 65.7 bits (153), Expect = 7e-12
Identities = 31/50 (62%), Positives = 37/50 (74%)
Frame = -1
Query: 803 TQSFQLYLETCLATLGDVXCIXQSYRAEQSRTRRHLAEYSHVEAECPFHN 654
TQS QLYLE L LG V I +S+RAE+S TRRHL+E++HVE E PF N
Sbjct: 316 TQSSQLYLEAALPALGSVYTIQESFRAEKSLTRRHLSEFTHVEFELPFVN 365
>SPBC1198.10c |||asparagine-tRNA ligase Slm5|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 441
Score = 83.8 bits (198), Expect = 3e-17
Identities = 53/153 (34%), Positives = 84/153 (54%), Gaps = 2/153 (1%)
Frame = -2
Query: 520 MTYIEAIEYLRANNITKEDGSFYEFGEDIPEMPERKMTDAIG-VPILLCKFPAEIKSFYM 344
MTY EAIE L A T + ++G D+ E+ + + + P+ + +P +IK FYM
Sbjct: 293 MTYSEAIEELSAVKKTWKYPP--KWGNDLSSEHEKYLCEILHKTPVFVTDYPQKIKPFYM 350
Query: 343 SRCVDDRRLTESVDVLMPGVGEIVGGSMRIWDHEELMEGYKREGIDPSPYYWYTDQRKFG 164
D +VD+L+P VGE+ GGS+R DH ++ YK P WY D K+
Sbjct: 351 KSSGPDT--VAAVDLLVPQVGELAGGSLRK-DH---LDEYKTY---PPELQWYLDLMKYS 401
Query: 163 SVPHGGYGLGLERFLCWLL-DRYHIRDVCLYPR 68
+ PHGG+GLG+ER + +L + ++++ +PR
Sbjct: 402 NAPHGGFGLGIERLIAFLEGENTNVKETIPFPR 434
Score = 44.4 bits (100), Expect = 2e-05
Identities = 24/48 (50%), Positives = 29/48 (60%)
Frame = -1
Query: 803 TQSFQLYLETCLATLGDVXCIXQSYRAEQSRTRRHLAEYSHVEAECPF 660
T S QL+LE L V I ++RAEQS T RHLAE+ +EAE F
Sbjct: 204 TVSTQLHLEALALGLSRVYTISPAFRAEQSHTSRHLAEFWMLEAEVAF 251
>SPCC1223.07c |||aspartate-tRNA ligase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 580
Score = 66.5 bits (155), Expect = 4e-12
Identities = 40/111 (36%), Positives = 57/111 (51%), Gaps = 1/111 (0%)
Frame = -2
Query: 376 KFPAEIKSFYMSRCVDDRRLTESVDVLMPGVGEIVGGSMRIWDHEELMEGYKREGIDPSP 197
K+P+ ++ FY +D R + S D M G EI+ G+ RI D E L+E K G+ P
Sbjct: 471 KYPSSVRPFYTMPDPEDPRYSNSYDFFMKGQ-EIMSGAQRIHDPELLVERMKALGVSPDV 529
Query: 196 -YYWYTDQRKFGSVPHGGYGLGLERFLCWLLDRYHIRDVCLYPRFLERCTP 47
Y D G PH G G+GLER + + L+ +IR +PR +R P
Sbjct: 530 GLQQYIDAFAIGCPPHAGGGIGLERVVMFYLNLPNIRLASSFPRDPKRLLP 580
Score = 31.5 bits (68), Expect = 0.14
Identities = 18/50 (36%), Positives = 29/50 (58%), Gaps = 1/50 (2%)
Frame = -1
Query: 803 TQSFQLYLETCLATLGD-VXCIXQSYRAEQSRTRRHLAEYSHVEAECPFH 657
+QS QLY + +A + V I +RAE S T RH+ E++ ++ E F+
Sbjct: 305 SQSPQLYKQMLIAADRERVFEIGPVFRAEDSNTYRHMTEFTGLDLEMAFN 354
>SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 591
Score = 36.3 bits (80), Expect = 0.005
Identities = 14/34 (41%), Positives = 24/34 (70%)
Frame = -2
Query: 172 KFGSVPHGGYGLGLERFLCWLLDRYHIRDVCLYP 71
++G P GG+G+G++R + +L D IR+V L+P
Sbjct: 542 EYGLPPTGGWGMGVDRLVMFLTDSNTIREVLLFP 575
>SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|chr
3|||Manual
Length = 611
Score = 32.3 bits (70), Expect = 0.083
Identities = 24/85 (28%), Positives = 40/85 (47%), Gaps = 6/85 (7%)
Frame = -2
Query: 304 DVLMPGVGEIVGGSMRIWDHEELMEGYKREGIDPSPYYWYTDQRKF------GSVPHGGY 143
D+++ G+ E+ GGS+RI H ++ + + + P Y G PHGG
Sbjct: 495 DIVVNGI-ELGGGSIRI--HNPDIQRFVLKDVLKLPENRYATFEHLIRVLSSGCPPHGGI 551
Query: 142 GLGLERFLCWLLDRYHIRDVCLYPR 68
LG +R L + IR+V +P+
Sbjct: 552 ALGFDRLAALLTNAPGIREVIAFPK 576
>SPAC9.03c |brr2|spp41|U5 snRNP complex subunit Brr2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2176
Score = 29.1 bits (62), Expect = 0.77
Identities = 11/30 (36%), Positives = 14/30 (46%)
Frame = +1
Query: 373 TCTAVSARQWRPSSSALAFPGCPLQTRRRI 462
TC + R W S FP CP + RR+
Sbjct: 1152 TCKMIEKRLWPTMSPLRQFPNCPSEVIRRV 1181
>SPAC1039.05c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 781
Score = 27.5 bits (58), Expect = 2.4
Identities = 9/22 (40%), Positives = 12/22 (54%)
Frame = -2
Query: 169 FGSVPHGGYGLGLERFLCWLLD 104
FG+ P Y + F+CWL D
Sbjct: 201 FGTSPFNAYQQNTDNFVCWLFD 222
>SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1067
Score = 27.5 bits (58), Expect = 2.4
Identities = 16/54 (29%), Positives = 29/54 (53%), Gaps = 2/54 (3%)
Frame = -2
Query: 313 ESVDVLMPGVGEIVGGSMRIWDHEELMEGYKREGIDPSPYYWYTD--QRKFGSV 158
++V + G+ EI+ + D E+L + Y +EG+ P WY D +R F ++
Sbjct: 989 KTVAAKLSGMKEILFPKSNLADWEQLPD-YVKEGLTGVPVAWYDDVFKRVFSNI 1041
>SPBP8B7.07c |set6||histone lysine methyltransferase Set6
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 483
Score = 27.1 bits (57), Expect = 3.1
Identities = 12/26 (46%), Positives = 16/26 (61%), Gaps = 3/26 (11%)
Frame = -2
Query: 169 FGSVPH---GGYGLGLERFLCWLLDR 101
FG + H G Y + L+ F+ WLLDR
Sbjct: 396 FGGLHHCILGRYSISLDDFVLWLLDR 421
>SPBC16G5.14c |rps3||40S ribosomal protein S3|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 249
Score = 26.6 bits (56), Expect = 4.1
Identities = 12/37 (32%), Positives = 19/37 (51%)
Frame = +2
Query: 65 EARVETHVPDVVPVQEPAEEALQTQPVAAVRHRAELA 175
+ R + +PD+V V +P EE T+P + E A
Sbjct: 196 KTRQKKSLPDIVVVLDPKEEEPITKPYTVINQPVEAA 232
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,884,953
Number of Sequences: 5004
Number of extensions: 55030
Number of successful extensions: 166
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 159
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 164
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 390427050
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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