Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= rbmnc16e17
(824 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 323 2e-89
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 323 2e-89
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 323 2e-89
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 102 7e-23
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 54 2e-08
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 54 2e-08
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 4.3
SPBC21D10.06c |map4||cell agglutination protein Map4|Schizosacch... 26 5.6
SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein... 26 7.5
SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificit... 25 9.9
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 25 9.9
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 323 bits (793), Expect = 2e-89
Identities = 147/188 (78%), Positives = 166/188 (88%)
Frame = -3
Query: 822 YKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVK 643
YKIGGIGTVPVGRVETGV+KPG IV FAPA +TTEVKSVEMHHE+L +PGDNVGFNVK
Sbjct: 252 YKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVK 311
Query: 642 NVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFA 463
NVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFA
Sbjct: 312 NVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFA 371
Query: 462 EIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTV 283
E+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTV
Sbjct: 372 ELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTV 431
Query: 282 AVGVIKAV 259
AVGVIKAV
Sbjct: 432 AVGVIKAV 439
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 323 bits (793), Expect = 2e-89
Identities = 147/188 (78%), Positives = 166/188 (88%)
Frame = -3
Query: 822 YKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVK 643
YKIGGIGTVPVGRVETGV+KPG IV FAPA +TTEVKSVEMHHE+L +PGDNVGFNVK
Sbjct: 252 YKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVK 311
Query: 642 NVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFA 463
NVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFA
Sbjct: 312 NVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFA 371
Query: 462 EIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTV 283
E+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTV
Sbjct: 372 ELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTV 431
Query: 282 AVGVIKAV 259
AVGVIKAV
Sbjct: 432 AVGVIKAV 439
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 323 bits (793), Expect = 2e-89
Identities = 147/188 (78%), Positives = 166/188 (88%)
Frame = -3
Query: 822 YKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVK 643
YKIGGIGTVPVGRVETGV+KPG IV FAPA +TTEVKSVEMHHE+L +PGDNVGFNVK
Sbjct: 252 YKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVK 311
Query: 642 NVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFA 463
NVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFA
Sbjct: 312 NVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFA 371
Query: 462 EIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTV 283
E+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTV
Sbjct: 372 ELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTV 431
Query: 282 AVGVIKAV 259
AVGVIKAV
Sbjct: 432 AVGVIKAV 439
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 102 bits (244), Expect = 7e-23
Identities = 60/190 (31%), Positives = 101/190 (53%), Gaps = 2/190 (1%)
Frame = -3
Query: 819 KIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSV-EMHHEALQEAVPGDNVGFNVK 643
K +GT+ G++E G +K + V+ P N T EV ++ + E + ++ GD V V+
Sbjct: 476 KYKDLGTILEGKIEAGSIKKNSNVLVMPINQTLEVTAIYDEADEEISSSICGDQVRLRVR 535
Query: 642 NVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFA 463
+++ GYV +KN P F AQ+ +L P ++ GY+ V+ HTA FA
Sbjct: 536 GDD-SDVQTGYVLTSTKN-PVHATTRFIAQIAILELPSILTTGYSCVMHIHTAVEEVSFA 593
Query: 462 EIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTV 283
++ K+D+ T + ++ P G I L P+C+E F+++ +GRF +RD TV
Sbjct: 594 KLLHKLDK-TNRKSKKPPMFATKGMKIIAELETQTPVCMERFEDYQYMGRFTLRDQGTTV 652
Query: 282 AVG-VIKAVN 256
AVG V+K ++
Sbjct: 653 AVGKVVKILD 662
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 54.4 bits (125), Expect = 2e-08
Identities = 49/176 (27%), Positives = 79/176 (44%), Gaps = 1/176 (0%)
Frame = -3
Query: 789 GRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQE-AVPGDNVGFNVKNVSVKELRRG 613
GRVE G ++ ++ + VK+V + + AV GD V + ++ V +LR G
Sbjct: 423 GRVEAGNVQVNQVLYDVSSQEDAYVKNVIRNSDPSSTWAVAGDTVTLQLADIEVNQLRPG 482
Query: 612 YVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRT 433
+ + +N P + F A++ + G I +G T VL H+ + K+
Sbjct: 483 DILSNYEN-PVRRVRSFVAEIQTFDIHGPILSGSTLVL-----HLGRTVTSVSLKIVTVN 536
Query: 432 GKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIK 265
K + + S K I L PLC+ +E P LGRF +R TVA G++K
Sbjct: 537 NKRSR-HIASRKRALVRISFLDGLFPLCLA--EECPALGRFILRRSGDTVAAGIVK 589
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 54.4 bits (125), Expect = 2e-08
Identities = 47/187 (25%), Positives = 81/187 (43%), Gaps = 2/187 (1%)
Frame = -3
Query: 822 YKIGGIGTVPVGRVETGVLKPGTIV--VFAPANITTEVKSVEMHHEALQEAVPGDNVGFN 649
+ I G GTV GRVE G LK G + V +++ T V +EM + L AV GDN G
Sbjct: 261 FSISGRGTVVTGRVERGTLKKGAEIEIVGYGSHLKTTVTGIEMFKKQLDAAVAGDNCGLL 320
Query: 648 VKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACK 469
++++ ++L+RG + P F A +L + T +D + + +
Sbjct: 321 LRSIKREQLKRGMIVAQPGTVAPH--QKFKASFYILTK--EEGGRRTGFVDKYRPQLYSR 376
Query: 468 FAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQ 289
+++ ++ T + K + GD + P+ +E Q RF VR+
Sbjct: 377 TSDVTVEL---THPDPNDSDKMVMPGDNVEMICTLIHPIVIEKGQ------RFTVREGGS 427
Query: 288 TVAVGVI 268
TV ++
Sbjct: 428 TVGTALV 434
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 26.6 bits (56), Expect = 4.3
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = +1
Query: 622 QFLDGHVLYVETYIVSRYSFLESFV 696
+F DGH+L ETY+ LES V
Sbjct: 527 KFFDGHLLASETYVGGHVESLESGV 551
>SPBC21D10.06c |map4||cell agglutination protein
Map4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 948
Score = 26.2 bits (55), Expect = 5.6
Identities = 13/32 (40%), Positives = 20/32 (62%)
Frame = +2
Query: 716 TSVVMLAGAKTTMVPGFNTPVSTLPTGTVPIP 811
+SVV+ + +T V + + VST TGTV +P
Sbjct: 86 SSVVLYSAKETVTVSSYWSLVSTSVTGTVYVP 117
>SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 670
Score = 25.8 bits (54), Expect = 7.5
Identities = 24/85 (28%), Positives = 42/85 (49%), Gaps = 2/85 (2%)
Frame = +2
Query: 215 SAALVTLPPPASLKLTALMTPTATVCLMSRTAKRPRGGNSWKDSTH--RGLEGTKLTMAA 388
S +VTLPPPAS ++ T T T + S ++ G+ + +++ + ++++
Sbjct: 183 STDIVTLPPPAS-STSSFSTITNTSMIPSSSSFTTTTGSPYYNTSSFLPSSVISSASLSS 241
Query: 389 SPDLMDFGLTSVDLPVRRSTFSLIS 463
S L +TS PV S+ SL S
Sbjct: 242 SSVLPTSIITSTSTPVTVSSSSLSS 266
>SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificity
factor complex subunit Rna14|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 733
Score = 25.4 bits (53), Expect = 9.9
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = +2
Query: 767 NTPVSTLPTGTVPIPPIL 820
N P S LPT VP+P I+
Sbjct: 666 NPPTSALPTVPVPLPSII 683
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 25.4 bits (53), Expect = 9.9
Identities = 11/25 (44%), Positives = 18/25 (72%)
Frame = +2
Query: 380 MAASPDLMDFGLTSVDLPVRRSTFS 454
++ +PDL D L+SVD P++ +T S
Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNTCS 51
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,425,921
Number of Sequences: 5004
Number of extensions: 71086
Number of successful extensions: 222
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 209
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 219
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 404442380
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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