Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= rbmnc16d04
         (758 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC337.05c |cct8||chaperonin-containing T-complex theta subunit...   191   1e-49
SPBC25H2.12c |cct7||chaperonin-containing T-complex eta subunit ...   121   1e-28
SPAC1D4.04 |cct2||chaperonin-containing T-complex beta subunit C...   105   7e-24
SPBC1A4.08c |cct3||chaperonin-containing T-complex gamma subunit...   105   9e-24
SPBC106.06 |cct4||chaperonin-containing T-complex delta subunit ...   103   3e-23
SPAC1420.02c |cct5||chaperonin-containing T-complex epsilon subu...   101   8e-23
SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subuni...    92   7e-20
SPBC646.11 |cct6||chaperonin-containing T-complex zeta subunit C...    69   1e-12
SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce...    27   3.8  
SPCC1450.16c |||triacylglycerol lipase|Schizosaccharomyces pombe...    26   6.7  
SPAC1F3.02c |mkh1||MEK kinase |Schizosaccharomyces pombe|chr 1||...    25   8.9  

>SPBC337.05c |cct8||chaperonin-containing T-complex theta subunit
           Cct8 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 546

 Score =  191 bits (465), Expect = 1e-49
 Identities = 86/198 (43%), Positives = 132/198 (66%), Gaps = 1/198 (0%)
 Frame = -3

Query: 756 QELGYCDAVHVDEVGDTRVVVFKMESSESRISTIVIRGSTDNYMDDIERAIDDGVNTFKG 577
           +E+G  D V   E+G  RV VF+     +R +TIV+RG+T  Y+DD+ERAIDDGVN  K 
Sbjct: 342 EEMGSVDVVETIEIGGDRVTVFRQVEDITRTATIVLRGATKTYLDDLERAIDDGVNIVKA 401

Query: 576 IARDGRFVPGAGATEIELAQQLLEFADTLPGLEQYAVRKFAVALESIPRALADNSGANAT 397
           + +D R + GAGA++++L  +L+   +  PG+ Q+A++++  A E +PR +++N+G + T
Sbjct: 402 LVKDNRLIFGAGASDMQLCIRLISVGEKTPGIYQHAIKQYGEAFEVVPRTISENAGLDPT 461

Query: 396 EVVNKIYKA-HKEGQKNAGFDIDSENNGVCDAKEKGIIDSYLLKFWGIKYAVGAATTILK 220
           +V++K+Y A HKE  ++ G D++ EN+G  DAKE GI D  L K   I+ A     T+L 
Sbjct: 462 DVISKLYAAHHKENGESIGVDVECENDGTLDAKEAGIFDVLLAKKSAIRLATETVLTVLN 521

Query: 219 VDQIIMAKRAGGPKPRAP 166
           VDQ++M+K AGGPKP  P
Sbjct: 522 VDQVVMSKPAGGPKPPGP 539


>SPBC25H2.12c |cct7||chaperonin-containing T-complex eta subunit
           Cct7|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 558

 Score =  121 bits (292), Expect = 1e-28
 Identities = 68/200 (34%), Positives = 115/200 (57%)
 Frame = -3

Query: 756 QELGYCDAVHVDEVGDTRVVVFKMESSESRISTIVIRGSTDNYMDDIERAIDDGVNTFKG 577
           + LG CD     ++G  R  +F+    +++  T+++RG  D ++ ++ER++ D +   K 
Sbjct: 345 KHLGTCDTFEERQIGGDRFNLFE-GCPKAKTCTLILRGGADQFIAEVERSLHDAIMIVKH 403

Query: 576 IARDGRFVPGAGATEIELAQQLLEFADTLPGLEQYAVRKFAVALESIPRALADNSGANAT 397
             ++   V G GA E+EL++ L +++ T+ G +Q  +  FA +LE IPR L DN+G ++T
Sbjct: 404 ALKNNLVVAGGGACEMELSKYLRDYSLTISGKQQNFIAAFARSLEVIPRQLCDNAGFDST 463

Query: 396 EVVNKIYKAHKEGQKNAGFDIDSENNGVCDAKEKGIIDSYLLKFWGIKYAVGAATTILKV 217
            ++NK+   H +G+  AG D+DSE  GV +  EK + +   +K   I  A  AAT IL V
Sbjct: 464 NILNKLRMQHAKGEMWAGVDMDSE--GVANNFEKFVWEPSTVKSNAILSATEAATLILSV 521

Query: 216 DQIIMAKRAGGPKPRAPAGS 157
           D+ I  K     +P+AP G+
Sbjct: 522 DETI--KNEPSQQPQAPQGA 539


>SPAC1D4.04 |cct2||chaperonin-containing T-complex beta subunit
           Cct2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 527

 Score =  105 bits (252), Expect = 7e-24
 Identities = 63/195 (32%), Positives = 105/195 (53%), Gaps = 1/195 (0%)
 Frame = -3

Query: 753 ELGYCDAVHVDEVGDTRVVVFK-MESSESRISTIVIRGSTDNYMDDIERAIDDGVNTFKG 577
           +LG+C  +    +G+ +++ F  +E+ E+   TIV+RG+T   +D+ ERAI D +     
Sbjct: 338 KLGHCKKIEEIIIGEDKMIKFSGVEAGEA--CTIVLRGATHQLLDESERAIHDALAVLSQ 395

Query: 576 IARDGRFVPGAGATEIELAQQLLEFADTLPGLEQYAVRKFAVALESIPRALADNSGANAT 397
              + R   G G  E+ +A+ + E A   PG +  AV  FA AL  +P  LADN+G +++
Sbjct: 396 TVAESRVTLGGGCAEMLMAKAVEEAATHEPGKKAVAVSAFAKALSQLPTILADNAGFDSS 455

Query: 396 EVVNKIYKAHKEGQKNAGFDIDSENNGVCDAKEKGIIDSYLLKFWGIKYAVGAATTILKV 217
           E+V ++  AH +G    G D+D     + D + KGI+++  LK   +      A  +L+V
Sbjct: 456 ELVAQLKAAHYDGNDTMGLDMD--EGEIADMRAKGILEALKLKQAVVSSGSEGAQLLLRV 513

Query: 216 DQIIMAKRAGGPKPR 172
           D I+ A     P+PR
Sbjct: 514 DTILKA----APRPR 524


>SPBC1A4.08c |cct3||chaperonin-containing T-complex gamma subunit
           Cct3|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 528

 Score =  105 bits (251), Expect = 9e-24
 Identities = 57/183 (31%), Positives = 97/183 (53%)
 Frame = -3

Query: 741 CDAVHVDEVGDTRVVVFKMESSESRISTIVIRGSTDNYMDDIERAIDDGVNTFKGIARDG 562
           C   ++D++GD     F       +  TI++RG + + ++++ER + D +   + +    
Sbjct: 345 CGLFYIDKLGD-EYYTFLTGCKNPKACTILLRGPSKDIINEVERNLQDAMAVARNVFFHP 403

Query: 561 RFVPGAGATEIELAQQLLEFADTLPGLEQYAVRKFAVALESIPRALADNSGANATEVVNK 382
           +  PG GATE+ ++ +L E A ++ G+ Q+  R  A A+E IPR L  N GAN  + + +
Sbjct: 404 KLSPGGGATEMAVSVRLAEKARSIEGVAQWPYRAVADAIEIIPRTLVQNCGANPIKALTE 463

Query: 381 IYKAHKEGQKNAGFDIDSENNGVCDAKEKGIIDSYLLKFWGIKYAVGAATTILKVDQIIM 202
           +   H EGQ +  F ID E   V D  E G+ +   +K   IK A+ +A  +L+VD I+ 
Sbjct: 464 LRAKHAEGQHS--FGIDGETGRVVDMHEYGVWEPEAVKLQSIKTAIESACLLLRVDDIVS 521

Query: 201 AKR 193
             R
Sbjct: 522 GVR 524


>SPBC106.06 |cct4||chaperonin-containing T-complex delta subunit
           Cct4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 527

 Score =  103 bits (247), Expect = 3e-23
 Identities = 56/186 (30%), Positives = 97/186 (52%)
 Frame = -3

Query: 753 ELGYCDAVHVDEVGDTRVVVFKMESSESRISTIVIRGSTDNYMDDIERAIDDGVNTFKGI 574
           +LG+ D V        ++V F    +  +  +I+ RG+    +++ ER++ D +   + +
Sbjct: 344 KLGHADLVEETSSSGEKIVKFSGVKNAGKTVSILCRGANLLTLEEAERSLHDALCVIRCL 403

Query: 573 ARDGRFVPGAGATEIELAQQLLEFADTLPGLEQYAVRKFAVALESIPRALADNSGANATE 394
            +    + G G+ EIE AQ+LLE A  L G E   +R F+ ALE IP  LA+N+G NA +
Sbjct: 404 VKQRALIAGGGSPEIEAAQRLLEHARQLEGREAICIRAFSEALEIIPVTLAENAGLNAIQ 463

Query: 393 VVNKIYKAHKEGQKNAGFDIDSENNGVCDAKEKGIIDSYLLKFWGIKYAVGAATTILKVD 214
           VV ++   H  G+K AG ++      V +  E+ ++   L+    I+ A      I+K+D
Sbjct: 464 VVTELRSRHANGEKTAGINV--RKGIVTNILEENVLQPLLVNISAIQLAAETTKMIMKID 521

Query: 213 QIIMAK 196
            I +A+
Sbjct: 522 DITLAR 527


>SPAC1420.02c |cct5||chaperonin-containing T-complex epsilon subunit
           Cct5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 546

 Score =  101 bits (243), Expect = 8e-23
 Identities = 61/186 (32%), Positives = 100/186 (53%), Gaps = 1/186 (0%)
 Frame = -3

Query: 753 ELGYCDAVHVDEVGDTRVVVFKMES-SESRISTIVIRGSTDNYMDDIERAIDDGVNTFKG 577
           +LG    V     G TR  +  +E  + SR  T+ +RGS    +D+ +RA+ D +   + 
Sbjct: 354 KLGSAGIVREVSFGTTRDKILVIEKCANSRAVTVFVRGSNKMIVDEAKRALHDALCVVRN 413

Query: 576 IARDGRFVPGAGATEIELAQQLLEFADTLPGLEQYAVRKFAVALESIPRALADNSGANAT 397
           + RD R V G GA EI  +  + + A+ +PG++QY++  FA AL++IP ALA+NSG ++ 
Sbjct: 414 LIRDNRVVYGGGAAEISCSLAVTKEAEKIPGIDQYSMGAFADALDTIPLALAENSGLSSI 473

Query: 396 EVVNKIYKAHKEGQKNAGFDIDSENNGVCDAKEKGIIDSYLLKFWGIKYAVGAATTILKV 217
           E +  + KA    +  A   ID    G  D +++ +ID  + K   +  A      +LKV
Sbjct: 474 EALTAV-KARHVKENKAYLGIDCLQTGSNDMRKQFVIDPLIGKKQQLLLATQLCRMVLKV 532

Query: 216 DQIIMA 199
           + II+A
Sbjct: 533 NDIIVA 538


>SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subunit
           Cct1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 556

 Score = 92.3 bits (219), Expect = 7e-20
 Identities = 63/194 (32%), Positives = 98/194 (50%), Gaps = 12/194 (6%)
 Frame = -3

Query: 750 LGYCDAVHVDEVGDTRVVVFKMESSESRISTIVIRGSTDNYMDDIERAIDDGVNTFKGIA 571
           LG  + V  ++  D   ++ K   + S  S IV+RG  +  +D++ER++ D ++  K   
Sbjct: 350 LGSAEEVVQEKFSDDECILVKGTKAYSSAS-IVLRGPNEYSLDEMERSMHDSLSVVKRTL 408

Query: 570 RDGRFVPGAGATEIELAQQLLEFADTLPGLEQYAVRKFAVALESIPRALADNSGANATEV 391
             G+ VPG GA E  L+  L  FA +L   EQ A+ +FA AL  IPR LA N+  ++TE+
Sbjct: 409 ESGKVVPGGGAVETALSIYLENFATSLGSREQLAIAEFAQALLIIPRTLAVNAAKDSTEL 468

Query: 390 VNKIYKAH------------KEGQKNAGFDIDSENNGVCDAKEKGIIDSYLLKFWGIKYA 247
             K+   H            K G KN G D+   N  + D  + G+++  + K   +K A
Sbjct: 469 TAKLRAYHAASQNAEVTDVKKRGYKNYGLDL--LNGVIRDNVKAGVLEPSMSKLKSLKSA 526

Query: 246 VGAATTILKVDQII 205
           V A   IL++D  I
Sbjct: 527 VEACIAILRIDTSI 540


>SPBC646.11 |cct6||chaperonin-containing T-complex zeta subunit
           Cct6|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 535

 Score = 68.5 bits (160), Expect = 1e-12
 Identities = 52/189 (27%), Positives = 89/189 (47%), Gaps = 2/189 (1%)
 Frame = -3

Query: 750 LGYCDAVHVDEVGDTRVVVFKMESSESRISTIVIRGSTDNYMDDIERAIDDGVNTFKGIA 571
           LG+  +V+   +G+ +   F  +  + + +TI+I G     +  I+ A  DG+   K   
Sbjct: 343 LGWAGSVYERTLGEEKYT-FVEDVKDPKSATILIHGPNTYTIQQIQDATRDGLRAVKNAV 401

Query: 570 RDGRFVPGAGATEIELAQQLLE--FADTLPGLEQYAVRKFAVALESIPRALADNSGANAT 397
            D   + GAGA E+  A  L     A  + G  +  V  +A AL  IP+ LA NS  +  
Sbjct: 402 EDNCLIVGAGAFEVACAAHLRNKFAAKEVKGKAKMGVYAYADALLIIPKTLAANSSYDTQ 461

Query: 396 EVVNKIYKAHKEGQKNAGFDIDSENNGVCDAKEKGIIDSYLLKFWGIKYAVGAATTILKV 217
           + +  + +   EG K  G D+  +     D + +GI D+Y +    +  A   A+ ++ V
Sbjct: 462 DAIVALQEEASEGYK-VGLDL--KTGMPFDPEVEGIYDNYRVIRHMLHSATVIASNLISV 518

Query: 216 DQIIMAKRA 190
           DQI+ A R+
Sbjct: 519 DQILRAGRS 527


>SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1036

 Score = 26.6 bits (56), Expect = 3.8
 Identities = 13/24 (54%), Positives = 16/24 (66%)
 Frame = +2

Query: 683 SILNTTTRVSPTSSTCTASQYPSS 754
           S  N+TT  SPTSS+ T+S   SS
Sbjct: 94  SSTNSTTSASPTSSSLTSSSATSS 117


>SPCC1450.16c |||triacylglycerol lipase|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 513

 Score = 25.8 bits (54), Expect = 6.7
 Identities = 14/39 (35%), Positives = 22/39 (56%), Gaps = 2/39 (5%)
 Frame = +2

Query: 257 IPQNFKRY--ESIMPFSFASQTPLFSESISKPAFFCPSL 367
           +P   +R+  +  +P ++ + TP FS S  K AF  PSL
Sbjct: 434 LPPRLRRFFVDDYVPSAYITLTPTFSFSDIKHAFTKPSL 472


>SPAC1F3.02c |mkh1||MEK kinase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1116

 Score = 25.4 bits (53), Expect = 8.9
 Identities = 20/79 (25%), Positives = 35/79 (44%)
 Frame = +2

Query: 104 IKSMLYQMMQTNI*DSSSLPAGALGFGPPALFAIMI*STLRMVVAAPTAYFIPQNFKRYE 283
           IKS +   +  N+ D S     A         AI + S +R  + AP++  +P     YE
Sbjct: 54  IKSRIEMDLFINLADQSKAEEFAFKNKLSKESAIQLSSCIRKTLLAPSSTRVPSKNSSYE 113

Query: 284 SIMPFSFASQTPLFSESIS 340
           ++   +  S   +F+E+ S
Sbjct: 114 TLTYSAKDSSDDVFTETNS 132


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,027,860
Number of Sequences: 5004
Number of extensions: 60490
Number of successful extensions: 195
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 178
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 190
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 363302114
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)