Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= rbmnc15m10
(650 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC19G7.10c |||topoisomerase associated protein |Schizosaccharo... 31 0.14
SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual 30 0.25
SPBC215.05 |gpd1||glycerol-3-phosphate dehydrogenase Gpd1|Schizo... 29 0.58
SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit Srb9... 27 3.1
SPCC4G3.08 |psk1||serine/threonine protein kinase Psk1|Schizosac... 27 3.1
SPAC13F5.01c |msh1|SPAC23C11.18c|MutS protein homolog 1|Schizosa... 26 4.1
SPAC227.11c |||sensor for misfolded ER glycoproteins Yos9 |Schiz... 26 4.1
SPBC4.03c |||COPII-coated vesicle component Sfb3 |Schizosaccharo... 26 5.4
SPBC342.06c |rtt109|kat11|RTT109 family histone lysine acetyltra... 25 7.2
SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomy... 25 7.2
SPAC821.13c ||SPAC955.01c|P-type ATPase |Schizosaccharomyces pom... 25 7.2
SPBC3D6.04c |mad1||mitotic spindle checkpoint protein Mad1|Schiz... 25 9.5
SPAP11E10.02c |mam3|SPAPB1A10.01c|cell agglutination protein Mam... 25 9.5
SPCC1322.12c |bub1||serine/threonine protein kinase Bub1|Schizos... 25 9.5
SPBC19F5.05c |ppp1|SPBC25D12.01c|pescadillo-family BRCT domain p... 25 9.5
>SPBC19G7.10c |||topoisomerase associated protein
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 744
Score = 31.1 bits (67), Expect = 0.14
Identities = 22/69 (31%), Positives = 34/69 (49%), Gaps = 2/69 (2%)
Frame = +3
Query: 405 PLNTINMDLNSLISKFAVPRVMVTLLDVLAAFGDLTP*SYTVPRKMLLVPTLLEPSLTMS 584
P+ + L L+S A R T L + A G + + PR++L V EP+ + S
Sbjct: 384 PMQKLQQQLQRLVSS-AKERPKATQLSLEGALGKIAVNTVRTPRQLLNVKRPTEPASSNS 442
Query: 585 SID--SGFS 605
S++ SGFS
Sbjct: 443 SLNNFSGFS 451
>SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1828
Score = 30.3 bits (65), Expect = 0.25
Identities = 16/50 (32%), Positives = 25/50 (50%), Gaps = 1/50 (2%)
Frame = +1
Query: 238 ELPRFSCTPECSW-ICAIRAPLVRTAAPCWGRSSTQSCCRLRIATCHCPL 384
++ RFS SW + +AP V + + C S ++SCC LR P+
Sbjct: 1769 DIDRFSLKMLESWGLFENKAPFVNSTSICTAVSESRSCCHLRYLNGAAPV 1818
>SPBC215.05 |gpd1||glycerol-3-phosphate dehydrogenase
Gpd1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 385
Score = 29.1 bits (62), Expect = 0.58
Identities = 14/40 (35%), Positives = 20/40 (50%), Gaps = 2/40 (5%)
Frame = +2
Query: 446 KVCG--AARHGHITRCAGRVWRFNSIVVYGAQKNAVSAHF 559
K+CG A HGH R R+W F + Y +K ++ F
Sbjct: 39 KICGENARAHGHHFRSKVRMWVFEEEIEYKGEKRKLTEVF 78
>SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit
Srb9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1223
Score = 26.6 bits (56), Expect = 3.1
Identities = 14/42 (33%), Positives = 23/42 (54%)
Frame = -2
Query: 586 DDIVKEGSNKVGTNSIFLGTVYDYGVKSPNAASTSSNVTMTR 461
D I + G NK S+ + T+ + SPN+ +T+SN T+
Sbjct: 891 DSIKRLGDNKFEDKSLRICTIPNSIFDSPNSHTTNSNSFFTK 932
>SPCC4G3.08 |psk1||serine/threonine protein kinase
Psk1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 436
Score = 26.6 bits (56), Expect = 3.1
Identities = 11/37 (29%), Positives = 20/37 (54%)
Frame = +2
Query: 314 PRVGVDQAHNHAVDFESQHAIVLYSFSGRYTFEYNKH 424
P + ++Q ++HAVD+ S ++ +G F N H
Sbjct: 258 PEILLEQPYDHAVDWWSMGILIFDLLTGSPPFTANNH 294
>SPAC13F5.01c |msh1|SPAC23C11.18c|MutS protein homolog
1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 941
Score = 26.2 bits (55), Expect = 4.1
Identities = 13/39 (33%), Positives = 24/39 (61%), Gaps = 2/39 (5%)
Frame = -2
Query: 430 KSMFI--VFKGITPTKTVEDNGMLRFEVDSMIVCLIDPN 320
K+M+I V + ITP +++N M +E + ++ + DPN
Sbjct: 156 KNMYIRSVTRVITPGTLIDENFMNPYESNYILTVVFDPN 194
>SPAC227.11c |||sensor for misfolded ER glycoproteins Yos9
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 322
Score = 26.2 bits (55), Expect = 4.1
Identities = 14/52 (26%), Positives = 26/52 (50%)
Frame = -2
Query: 583 DIVKEGSNKVGTNSIFLGTVYDYGVKSPNAASTSSNVTMTRGTANFDIKEFK 428
D +KE + K +S+F ++Y PNA+ N T ++D++E +
Sbjct: 64 DSIKEKTEKTKLSSLFYAGKHEYFCVYPNASLIKQNSTT---EPSYDLQELR 112
>SPBC4.03c |||COPII-coated vesicle component Sfb3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 891
Score = 25.8 bits (54), Expect = 5.4
Identities = 12/31 (38%), Positives = 19/31 (61%)
Frame = +3
Query: 507 LTP*SYTVPRKMLLVPTLLEPSLTMSSIDSG 599
LTP +P+ ++L+P L+ L S+I SG
Sbjct: 673 LTPGQLVLPKNLVLLPILILAVLKSSAIRSG 703
>SPBC342.06c |rtt109|kat11|RTT109 family histone lysine
acetyltransferase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 369
Score = 25.4 bits (53), Expect = 7.2
Identities = 8/18 (44%), Positives = 13/18 (72%)
Frame = +1
Query: 238 ELPRFSCTPECSWICAIR 291
ELPRF P+C ++C ++
Sbjct: 212 ELPRFPDDPKCRYLCELQ 229
>SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 541
Score = 25.4 bits (53), Expect = 7.2
Identities = 14/34 (41%), Positives = 21/34 (61%)
Frame = +3
Query: 459 PRVMVTLLDVLAAFGDLTP*SYTVPRKMLLVPTL 560
P V++ V AF +TP YTVP+ +++PTL
Sbjct: 384 PPVLMVPYRVKKAF-PITP-DYTVPKDAMVIPTL 415
>SPAC821.13c ||SPAC955.01c|P-type ATPase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1562
Score = 25.4 bits (53), Expect = 7.2
Identities = 17/58 (29%), Positives = 30/58 (51%)
Frame = -2
Query: 640 HGDNKLSQMYIAEKPLSIDDIVKEGSNKVGTNSIFLGTVYDYGVKSPNAASTSSNVTM 467
+G KL + E PLS +D++ GSN T+ +F ++ G +S + NV++
Sbjct: 508 NGSMKLDSIQ-GEIPLSNNDVLYRGSNLRNTSELFALVIFT-GEESKIRMNAVRNVSV 563
>SPBC3D6.04c |mad1||mitotic spindle checkpoint protein
Mad1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 689
Score = 25.0 bits (52), Expect = 9.5
Identities = 13/38 (34%), Positives = 21/38 (55%)
Frame = -2
Query: 403 ITPTKTVEDNGMLRFEVDSMIVCLIDPNTGPLSEREVR 290
+T T + EDN F+ +S + L+ +GP ER +R
Sbjct: 627 VTSTYSREDNTAFIFDGESSTMKLVGNPSGPEFERLIR 664
>SPAP11E10.02c |mam3|SPAPB1A10.01c|cell agglutination protein
Mam3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1082
Score = 25.0 bits (52), Expect = 9.5
Identities = 15/57 (26%), Positives = 30/57 (52%)
Frame = +3
Query: 294 TSRSDSGPVLGSIKHTIMLSTSNRNMPLSSTVLVGVIPLNTINMDLNSLISKFAVPR 464
T+ SDS + ++ +++ S+ N+P SS+ LV + + + L+ L F+ R
Sbjct: 295 TATSDSSVITYTLINSVTSSSETTNLPSSSSSLVTIGESSFPSSLLSLLTQSFSTVR 351
>SPCC1322.12c |bub1||serine/threonine protein kinase
Bub1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1044
Score = 25.0 bits (52), Expect = 9.5
Identities = 12/43 (27%), Positives = 20/43 (46%)
Frame = -2
Query: 610 IAEKPLSIDDIVKEGSNKVGTNSIFLGTVYDYGVKSPNAASTS 482
I +PL + + K + + S +LGT S N+ +TS
Sbjct: 396 IFNQPLRSESLEKSSKSPISAQSSYLGTPLKNDENSSNSGATS 438
>SPBC19F5.05c |ppp1|SPBC25D12.01c|pescadillo-family BRCT domain
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 607
Score = 25.0 bits (52), Expect = 9.5
Identities = 13/31 (41%), Positives = 17/31 (54%)
Frame = -2
Query: 646 IAHGDNKLSQMYIAEKPLSIDDIVKEGSNKV 554
I HG+NK ++ IA I DIV +N V
Sbjct: 274 IVHGNNKNARKNIATLKSKIRDIVNSDANVV 304
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,683,883
Number of Sequences: 5004
Number of extensions: 56541
Number of successful extensions: 153
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 146
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 153
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 293780908
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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