BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= rbmnc14h05
(772 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC27F1.07 |||dolichyl-diphospho-oligosaccharide-protein glycos... 86 6e-18
SPBC530.15c ||SPBC661.01|spermidine family transporter |Schizosa... 31 0.18
SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomy... 29 0.74
SPBC36.06c |spo9||farnesyl pyrophosphate synthetase|Schizosaccha... 28 1.7
SPCC306.09c |cap1|cap|adenylyl cyclase-associated protein Cap1|S... 28 1.7
SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosacchar... 27 3.9
SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase Mok11|S... 26 6.9
SPAC926.09c |fas1||fatty acid synthase beta subunit Fas1|Schizos... 26 6.9
SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces... 26 6.9
SPAC29B12.05c |||mitochondrial S-adenosylmethionine-dependent me... 26 6.9
>SPAC27F1.07 |||dolichyl-diphospho-oligosaccharide-protein
glycosyltransferase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 450
Score = 85.8 bits (203), Expect = 6e-18
Identities = 47/147 (31%), Positives = 76/147 (51%), Gaps = 1/147 (0%)
Frame = -3
Query: 770 PASASDVYYRDTNGNISTSNMKVKKDSVELDLRPRYPLFGGWRTHYTLGYNVPSYEYLYH 591
P ++ DVYY D GNI+TS+M+V+ ++L PRYP+FGGW + L + +P YE
Sbjct: 276 PRNSKDVYYTDEVGNITTSHMRVEPHQTWIELNPRYPVFGGWNYLFQLDWKMP-YEEFRS 334
Query: 590 SGNEYLLKMRSIDHIFDDMQVDELVTKIILPEGSTGIKLNIPYSVTRLPDSLHFTYLDTK 411
S N + DM ++ V + PEG+T I++ +P ++ + +LDT
Sbjct: 335 SKNRREYLDIPLAWSPGDMIYEKAVWSYVFPEGATNIEIEMPVEISNSNVTKIHKFLDTL 394
Query: 410 GRPVISFIKKNIVENHIQDF-QIRYTF 333
GR V ++ N+ + D I Y +
Sbjct: 395 GRQVFTYEALNVTDGVPSDIVHISYEY 421
>SPBC530.15c ||SPBC661.01|spermidine family transporter
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 516
Score = 31.1 bits (67), Expect = 0.18
Identities = 15/37 (40%), Positives = 23/37 (62%)
Frame = -3
Query: 353 FQIRYTFPRLLMLQEPLLVVGFLYTLFLCVIIYVRLD 243
F +TFP L++ EP+L+V YT F+ I+Y L+
Sbjct: 291 FHKYFTFPLRLLIFEPILLVVSTYTAFVYGILYGLLE 327
>SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 321
Score = 29.1 bits (62), Expect = 0.74
Identities = 34/152 (22%), Positives = 63/152 (41%), Gaps = 4/152 (2%)
Frame = -3
Query: 746 YRDTNGNISTSNMKVKKDSVELDLRPRYPLFGGWRTHYTLGYNVPSYEYLYHSGNEYLLK 567
+ DTN KVK +++L P P H LG +V +Y GN+ +
Sbjct: 169 FNDTNLERILKVAKVKPAVHQMELHPFLPQTEFVEKHKKLGIHVTAYSPF---GNQNTIY 225
Query: 566 MRSIDHIFDDMQVDELVTKIILPEGSTGIKLNIPYSVTR----LPDSLHFTYLDTKGRPV 399
I + + + ++ EG TG + + +++TR +P S++ + + +
Sbjct: 226 ESKIPKLIEHETIQKIAKS--KGEGVTGATIAVSWAITRGTSVIPKSVNEQRIKSNFK-Y 282
Query: 398 ISFIKKNIVENHIQDFQIRYTFPRLLMLQEPL 303
I K+++ E I IR F + EP+
Sbjct: 283 IPLTKEDMDE--INSIGIRARFNQATFSNEPV 312
>SPBC36.06c |spo9||farnesyl pyrophosphate
synthetase|Schizosaccharomyces pombe|chr 2|||Manual
Length = 351
Score = 27.9 bits (59), Expect = 1.7
Identities = 19/58 (32%), Positives = 29/58 (50%), Gaps = 2/58 (3%)
Frame = -3
Query: 644 RTHYTLG--YNVPSYEYLYHSGNEYLLKMRSIDHIFDDMQVDELVTKIILPEGSTGIK 477
R HY N+ + LYH L++ + H F+D VD + +I L + STG+K
Sbjct: 283 RAHYGKAGSENIAVIKQLYHE-----LQIPELYHKFEDDMVDSISKEIDLIDESTGLK 335
>SPCC306.09c |cap1|cap|adenylyl cyclase-associated protein
Cap1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 551
Score = 27.9 bits (59), Expect = 1.7
Identities = 15/53 (28%), Positives = 29/53 (54%)
Frame = +3
Query: 6 PLFYLQSM***STGLFHEGQTLKYCFIKSEIKIYFIKNYLFLHTEIITTKNAH 164
PL ++ M + F+ + +K K +++I ++++YL L TE+IT H
Sbjct: 214 PLSFMSEMK--DSSQFYANRVMKEFKGKDDLQIEWVRSYLTLLTELITYVKTH 264
>SPAC25B8.04c |||mitochondrial splicing suppressor
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 378
Score = 26.6 bits (56), Expect = 3.9
Identities = 14/30 (46%), Positives = 16/30 (53%), Gaps = 4/30 (13%)
Frame = -2
Query: 366 PYPRFSNPLY----IPTSADVAGTTFSCWL 289
P P S+PLY IPT D+ T S WL
Sbjct: 277 PMPLISHPLYSSSWIPTLHDLVSTRCSVWL 306
>SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase
Mok11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2397
Score = 25.8 bits (54), Expect = 6.9
Identities = 20/76 (26%), Positives = 34/76 (44%), Gaps = 1/76 (1%)
Frame = -3
Query: 578 YLLKMRSIDHIFDDMQVDELVTKIILPEGSTGIKLNIPYSVTRLPDSLHFTYLDTKGRPV 399
Y ++ + H+ Q +E + + S KL ++ R P S + LDT R
Sbjct: 1557 YTIESNTTAHLL--CQFEEACRQALTSSKSVRTKLRAISTIQRFPVSEWVSKLDTHVRNC 1614
Query: 398 ISFI-KKNIVENHIQD 354
I F K+N+ E+ I +
Sbjct: 1615 IKFSHKQNLEEDFIHE 1630
>SPAC926.09c |fas1||fatty acid synthase beta subunit
Fas1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2073
Score = 25.8 bits (54), Expect = 6.9
Identities = 10/21 (47%), Positives = 14/21 (66%)
Frame = -3
Query: 440 SLHFTYLDTKGRPVISFIKKN 378
S+ F +D G PVI F+K+N
Sbjct: 1511 SVDFQSVDCHGNPVIEFLKRN 1531
>SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3699
Score = 25.8 bits (54), Expect = 6.9
Identities = 21/76 (27%), Positives = 37/76 (48%), Gaps = 5/76 (6%)
Frame = -3
Query: 599 LYHSGNEYLLKMR---SIDHIFDDMQ-VDELV-TKIILPEGSTGIKLNIPYSVTRLPDSL 435
LY + E L+ R S D++ + VD+L+ TKI++ G T P + + L D L
Sbjct: 356 LYQTRRELLVATRHVLSTDYLRGFLPYVDQLLDTKILVGSGITSQHSLRPMAFSMLADML 415
Query: 434 HFTYLDTKGRPVISFI 387
H+ ++ + + I
Sbjct: 416 HYVRMELSPQQIYKVI 431
>SPAC29B12.05c |||mitochondrial S-adenosylmethionine-dependent
methyltransferase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 309
Score = 25.8 bits (54), Expect = 6.9
Identities = 10/27 (37%), Positives = 16/27 (59%)
Frame = +2
Query: 164 LTRCVKSGNNMFHYKFNLYVQTCELKN 244
L K+G N F+YKF+ Y++ +N
Sbjct: 224 LALLAKNGGNEFYYKFSQYIKRMLQRN 250
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,343,384
Number of Sequences: 5004
Number of extensions: 73710
Number of successful extensions: 193
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 188
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 192
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 371330890
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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