SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= rbmnc13h08
         (692 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC30C2.04 |||cofactor for methionyl-and glutamyl-tRNA syntheta...   143   2e-35
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce...    29   0.48 
SPCP1E11.06 |apl4||AP-1 adaptor complex gamma subunit Apl4 |Schi...    27   3.4  
SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosacc...    27   3.4  
SPAC19D5.06c |din1||Dhp1p-interacting protein Din1|Schizosacchar...    27   3.4  
SPAC2F7.16c |||phospholipase D |Schizosaccharomyces pombe|chr 1|...    27   3.4  
SPBC3D6.12 |||U3 snoRNA associted protein Dip2 |Schizosaccharomy...    25   7.9  
SPBC359.02 |alr2||alanine racemase Alr2 |Schizosaccharomyces pom...    25   7.9  
SPAC1565.03 |||sequence orphan|Schizosaccharomyces pombe|chr 1||...    25   7.9  

>SPAC30C2.04 |||cofactor for methionyl-and glutamyl-tRNA synthetases
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 450

 Score =  143 bits (346), Expect = 2e-35
 Identities = 73/139 (52%), Positives = 97/139 (69%), Gaps = 4/139 (2%)
 Frame = -3

Query: 462 LDFRIGKIVEISKHPDADSLYVEKIDCGE-ENPRTVVSGLVNHVPIDEMRERIVMVLCNL 286
           +DFRIG I +  KHP+ADSLYV  I CG+ E PRTV SGLV ++P+++M++R V+V+ NL
Sbjct: 282 IDFRIGFIEKAVKHPNADSLYVSTIHCGDAEGPRTVCSGLVKYIPLEQMQQRKVIVVANL 341

Query: 285 KPVKMRGVTSEAMVMCASSAEK--VEVLIPPPDAIPGDLVVCEGY-PREPEAVLNPKKKI 115
           KPV MR V S+AMV CASS +K  VE ++PP +A  GD +  EG+   EPEA LNPK+KI
Sbjct: 342 KPVNMRSVKSQAMVFCASSPDKSVVEFVLPPENAEIGDRLTFEGFDTEEPEAQLNPKRKI 401

Query: 114 FETCAPDLKTNNEKIACYK 58
           +E   P   +  + I  YK
Sbjct: 402 WEAIQPGFTSGEDLICGYK 420


>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1666

 Score = 29.5 bits (63), Expect = 0.48
 Identities = 14/41 (34%), Positives = 23/41 (56%)
 Frame = -3

Query: 429 SKHPDADSLYVEKIDCGEENPRTVVSGLVNHVPIDEMRERI 307
           SK P      ++ IDC EE  + ++  +V  VP+DE+ E +
Sbjct: 807 SKTPQVVGALLD-IDCDEELVQNLLMSVVGQVPVDELVEEV 846


>SPCP1E11.06 |apl4||AP-1 adaptor complex gamma subunit Apl4
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 865

 Score = 26.6 bits (56), Expect = 3.4
 Identities = 10/16 (62%), Positives = 12/16 (75%)
 Frame = -3

Query: 432 ISKHPDADSLYVEKID 385
           I K PD +SLY+EK D
Sbjct: 193 IQKEPDLESLYIEKTD 208


>SPAC688.08 |srb8|med12|mediator complex subunit Srb8
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1233

 Score = 26.6 bits (56), Expect = 3.4
 Identities = 14/43 (32%), Positives = 22/43 (51%), Gaps = 1/43 (2%)
 Frame = -3

Query: 411  DSLYVEKIDCGEENPRTVVSGLVNHVPI-DEMRERIVMVLCNL 286
            DS ++EK+ C         S L NH+ + D  R+  +  LC+L
Sbjct: 1001 DSWFLEKLPCPLTQSLQSFSHLSNHIEVLDSTRQSRLTFLCHL 1043


>SPAC19D5.06c |din1||Dhp1p-interacting protein
           Din1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 352

 Score = 26.6 bits (56), Expect = 3.4
 Identities = 11/34 (32%), Positives = 19/34 (55%)
 Frame = -2

Query: 358 CVWFSKPCSNR*NARKNSYGTLQLETS*DERCNI 257
           C+W  KPCS + +   +  GT++ + S  E  N+
Sbjct: 202 CIWDKKPCSAKESDVHSDDGTIEEDASNAENPNL 235


>SPAC2F7.16c |||phospholipase D |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1369

 Score = 26.6 bits (56), Expect = 3.4
 Identities = 9/19 (47%), Positives = 12/19 (63%)
 Frame = -3

Query: 249 MVMCASSAEKVEVLIPPPD 193
           ++ C   A K  +LIPPPD
Sbjct: 753 LIQCKKPARKTPLLIPPPD 771


>SPBC3D6.12 |||U3 snoRNA associted protein Dip2 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 922

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 14/33 (42%), Positives = 20/33 (60%), Gaps = 2/33 (6%)
 Frame = +2

Query: 122 FLGLSTASGS--LGYPSHTTKSPGIASGGGIKT 214
           F G+  +SG   LG PS + KS G A  GG+++
Sbjct: 13  FFGVIASSGCNILGQPSSSAKSVGRAIVGGLES 45


>SPBC359.02 |alr2||alanine racemase Alr2 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 370

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 16/54 (29%), Positives = 30/54 (55%), Gaps = 2/54 (3%)
 Frame = -3

Query: 483 IAVDVRKL-DFRIGKIVEISKHPDADSLYVEKIDC-GEENPRTVVSGLVNHVPI 328
           +A+DV  + D + G  V +  +P+   L +E++     ENP  +++GL   VP+
Sbjct: 316 LAIDVTDVPDVKRGDDVVLWGNPE---LTIEEVSTFSNENPFEIITGLTRRVPL 366


>SPAC1565.03 |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 166

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 14/28 (50%), Positives = 18/28 (64%), Gaps = 2/28 (7%)
 Frame = -1

Query: 566 KNQLSIKRRRKSKILT--KNPKINLVNR 489
           KN+   KRR+ SK+LT   N +I  VNR
Sbjct: 77  KNEHFSKRRKLSKVLTPPTNDEIKFVNR 104


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,848,898
Number of Sequences: 5004
Number of extensions: 58028
Number of successful extensions: 157
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 148
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 154
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 321951680
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -