Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= rbmnc13d22
         (769 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC23G3.12c |||serine protease |Schizosaccharomyces pombe|chr 1...    39   0.001
SPBC1685.05 |||serine protease |Schizosaccharomyces pombe|chr 2|...    36   0.006
SPBC31A8.01c |cwl1|rtn1, SPBC651.13c|reticulon-like protein|Schi...    28   1.3  
SPAC19A8.01c |sec73|sec7c, SPAC23H3.01|guanyl-nucleotide exchang...    28   1.7  
SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr 1|||M...    27   3.9  
SPCC4G3.18 |||conserved fungal family|Schizosaccharomyces pombe|...    27   3.9  
SPBC1105.08 |||EMP70 family|Schizosaccharomyces pombe|chr 2|||Ma...    27   3.9  
SPBC8D2.14c |sed5||SNARE Sed5 |Schizosaccharomyces pombe|chr 2||...    27   3.9  
SPBC29A3.11c |||mitochondrial carboxylic acid transporter|Schizo...    26   5.2  
SPAC12G12.15 |sif3||Sad1 interacting factor 3|Schizosaccharomyce...    26   6.8  

>SPAC23G3.12c |||serine protease |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 996

 Score = 38.7 bits (86), Expect = 0.001
 Identities = 42/174 (24%), Positives = 79/174 (45%), Gaps = 9/174 (5%)
 Frame = -1

Query: 712 ELGLQDRNIVYIQTDAPITFGNSGGPLVNLDGEAIGINS-MKVTYGISFAIPIDYVKEFL 536
           EL   D N  YIQ  A  + G+SG P+V  +G  + + +   +     + +P+D     L
Sbjct: 194 ELTYCDFNTNYIQAAANASGGSSGSPVVERNGNVVALQAGGHMIAATDYFLPLDRPLRAL 253

Query: 535 AKHKTKSP----QVSKRYLGITM---LSLTPSILMELKMRNPEMPTDIQHGILVWKVIIG 377
              +  +P     +  ++L  T      L     ME K+R   +  +    ++V  V+  
Sbjct: 254 RCLQNNTPITRGTIQAQFLIKTFDECSRLGLDSAMEEKVRT--LFPEATSMLVVETVLPE 311

Query: 376 SPAFNGGLQPGDIVVKINGKPVHNTTDIYNILESTTG-SLKIDAVRGRQQINLT 218
            P+F   L+ GDI++ +N   + N  ++ +IL+ + G  + +   RG + + LT
Sbjct: 312 GPSFK-KLKEGDILLYVNSMILINLIELESILDESVGKDVVLTVQRGSELVELT 364


>SPBC1685.05 |||serine protease |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 997

 Score = 35.9 bits (79), Expect = 0.006
 Identities = 31/128 (24%), Positives = 61/128 (47%), Gaps = 6/128 (4%)
 Frame = -1

Query: 697 DRNIVYIQTDAPITFGNSGGPLVNLDGEAIGINS-MKVTYGISFAIPIDYVKEFLAKHKT 521
           D N  Y Q  +  + G+SG P++++ G A+ +NS    +   SF +P+D V   L   + 
Sbjct: 214 DFNTFYYQAASGTSGGSSGSPVLDISGAAVALNSGGSNSSASSFYLPLDRVVRALRCIEN 273

Query: 520 KSPQVSKRYLGITMLSLTPSILMELKMRNP---EMPTDI--QHGILVWKVIIGSPAFNGG 356
            +P +++  L    L  +   L  + +      +  T +    G+LV   ++ +   +  
Sbjct: 274 NTP-ITRGTLLTEFLHWSYDELSRIGLPREFEYDCRTRVPSSTGLLVVSRVLRNSEVSKA 332

Query: 355 LQPGDIVV 332
           L+PGDI++
Sbjct: 333 LEPGDILI 340



 Score = 30.3 bits (65), Expect = 0.32
 Identities = 16/62 (25%), Positives = 33/62 (53%), Gaps = 2/62 (3%)
 Frame = -1

Query: 346 GDIVVKINGKPVHNTTDIYNILESTTGSLKIDAVRG--RQQINLTIVPELH*YSLRRVFF 173
           GDI++++NGK +   +D++     T   +K   +R     +I + + PE   +S R + +
Sbjct: 814 GDILLEVNGKMITRLSDLHEF--ETESEIKAVILRDGIEMEITIPLYPEYPTFSSRAICW 871

Query: 172 LG 167
           +G
Sbjct: 872 MG 873


>SPBC31A8.01c |cwl1|rtn1, SPBC651.13c|reticulon-like
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 308

 Score = 28.3 bits (60), Expect = 1.3
 Identities = 14/44 (31%), Positives = 24/44 (54%)
 Frame = +3

Query: 363 LKAGDPMMTFHTRIPC*ISVGISGFLIFNSMSMEGVNESMVMPR 494
           L A  P++TF   +   I   +SGFL + S+ +  V  + ++PR
Sbjct: 228 LFAESPILTFTASVAAFIEFFLSGFLSYKSLFVWNVLFAFILPR 271


>SPAC19A8.01c |sec73|sec7c, SPAC23H3.01|guanyl-nucleotide exchange
           factor Sec73 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1082

 Score = 27.9 bits (59), Expect = 1.7
 Identities = 27/114 (23%), Positives = 46/114 (40%), Gaps = 2/114 (1%)
 Frame = -1

Query: 763 SNTVTAGVVSXTQRAGSELGLQDRNIVYIQTDAPITFGNSGGPLVNLDGEAIGINSMKVT 584
           S T +   V    +AG  L  Q+ N+   ++D  I    +    V +      I S+ V 
Sbjct: 175 STTSSPSEVGSLDKAGGPLNQQNENLS-AESDNTILQNGTRKSSVKMSDLGKRIASLPVV 233

Query: 583 YG--ISFAIPIDYVKEFLAKHKTKSPQVSKRYLGITMLSLTPSILMELKMRNPE 428
               I F+I  DY+K      +T +       +G   L  +PSI  +++   P+
Sbjct: 234 KRPPIQFSISSDYIKPVDKSDQTSNVPSPSSSVGSLRLYRSPSIPNDVEQPQPQ 287


>SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 500

 Score = 26.6 bits (56), Expect = 3.9
 Identities = 22/88 (25%), Positives = 44/88 (50%), Gaps = 5/88 (5%)
 Frame = -3

Query: 365 QRGSTTRRHCSQNKRKTSSQYNRYIQHS-RKYDGEFED*RRQRA-PA--DKPDHCSRVTL 198
           + GS+     S++   +SS+       S  + +   ED ++++A PA  ++P   ++ + 
Sbjct: 198 KEGSSESSSDSESSSDSSSESGDSDSSSDSESESSSEDEKKRKAEPASEERPAKITKPSQ 257

Query: 197 I*SSTC-FFFGRAYGSIDDEWLPSFMED 117
             + TC  F GR   ++DD+WL    E+
Sbjct: 258 DSNETCTVFVGRLSWNVDDQWLGQEFEE 285


>SPCC4G3.18 |||conserved fungal family|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 828

 Score = 26.6 bits (56), Expect = 3.9
 Identities = 16/46 (34%), Positives = 22/46 (47%), Gaps = 1/46 (2%)
 Frame = -1

Query: 163 HTAQLMMSGCRRSWKTVISKAEPSHCLLNKHSNPS-YNAVFFKRFQ 29
           H   L+ S  R S +T+ S   P    L KH +PS +   F  RF+
Sbjct: 555 HPDPLVASHARSSMQTIESLIHPRFPPLQKHLSPSEFENTFESRFE 600


>SPBC1105.08 |||EMP70 family|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 629

 Score = 26.6 bits (56), Expect = 3.9
 Identities = 13/28 (46%), Positives = 19/28 (67%)
 Frame = +3

Query: 279 SRMLYISVVL*TGFPFILTTMSPGCRPP 362
           S ++++SV+L T F F LT +SP   PP
Sbjct: 12  SFVVFVSVLLQTCFSFQLTPLSPKNYPP 39


>SPBC8D2.14c |sed5||SNARE Sed5 |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 309

 Score = 26.6 bits (56), Expect = 3.9
 Identities = 12/29 (41%), Positives = 16/29 (55%)
 Frame = +1

Query: 124 MNDGNHSSSIEPYARPKKKHVEDYISVTL 210
           +N GN  S    Y  PK +  EDY+S+ L
Sbjct: 171 INSGNSISPFADYNDPKPEANEDYLSLNL 199


>SPBC29A3.11c |||mitochondrial carboxylic acid
           transporter|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 297

 Score = 26.2 bits (55), Expect = 5.2
 Identities = 14/35 (40%), Positives = 20/35 (57%)
 Frame = +3

Query: 48  TALYEGLLCLLSRQWLGSAFEITVFHERRQPLIIN 152
           TAL+ G    L+R  LGSA   T++   ++ LI N
Sbjct: 175 TALWNGYRYHLTRDALGSACYFTIYETFKKNLIAN 209


>SPAC12G12.15 |sif3||Sad1 interacting factor 3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 510

 Score = 25.8 bits (54), Expect = 6.8
 Identities = 8/23 (34%), Positives = 18/23 (78%)
 Frame = -2

Query: 429 RCQPIFNTVSLYGRSSLDLRLST 361
           RC+ IF+ + ++G+SS D ++++
Sbjct: 421 RCKVIFDMLDIFGKSSADRKMNS 443


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,346,478
Number of Sequences: 5004
Number of extensions: 74418
Number of successful extensions: 224
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 212
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 224
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 369323696
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)