BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= rbmnc13a05
(820 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1687.10 |mcp1||sequence orphan|Schizosaccharomyces pombe|chr... 33 0.049
SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|... 29 0.79
SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyce... 29 1.0
SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces pomb... 29 1.0
SPCC63.11 |prp28||U5 snRNP-associated protein Prp28 |Schizosacch... 28 1.8
SPAC144.14 |klp8||kinesin-like protein Klp8|Schizosaccharomyces ... 28 1.8
SPBC365.05c |slu7||splicing factor Slu7|Schizosaccharomyces pomb... 27 2.4
SPCC162.08c |nup211||nuclear pore complex associated protein|Sch... 27 2.4
SPBC21D10.10 |||bromodomain protein|Schizosaccharomyces pombe|ch... 27 3.2
SPCC970.10c |brl2|rfp1|ubiquitin-protein ligase E3 Brl2|Schizosa... 26 5.6
SPAC27D7.02c |||GRIP domain protein|Schizosaccharomyces pombe|ch... 26 5.6
SPBC29A3.13 |||PWWP domain protein|Schizosaccharomyces pombe|chr... 26 7.4
SPAC1250.05 |rpl3002|rpl30-2, rpl30|60S ribosomal protein L30|Sc... 25 9.8
>SPAC1687.10 |mcp1||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 661
Score = 33.1 bits (72), Expect = 0.049
Identities = 23/79 (29%), Positives = 46/79 (58%), Gaps = 3/79 (3%)
Frame = -3
Query: 785 KQNDVKNLEEAVEELS--LADDSEKIPYLIGEIFICQNLEITLKNLEEAKAKKIHEIHDL 612
K+N+ KN+ + +E LS L + +++ ++ G+ +I L+ L AK ++H+I+ L
Sbjct: 106 KKNEAKNMYKEIERLSVILGESVDEVNFVNGDFEDIS--DIKLEQL-SAKLNELHQIYRL 162
Query: 611 -EEKCEELKSQMSDLKAHL 558
+EK E+K ++ L+ L
Sbjct: 163 RKEKNIEIKEELKKLQDSL 181
>SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase
Sen1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1687
Score = 29.1 bits (62), Expect = 0.79
Identities = 24/77 (31%), Positives = 41/77 (53%), Gaps = 3/77 (3%)
Frame = -3
Query: 803 KDELKVKQN--DVKNLEEAVE-ELSLADDSEKIPYLIGEIFICQNLEITLKNLEEAKAKK 633
K L+V Q D+ +L+E + D +KI L +I + +++ K+L + K
Sbjct: 1251 KQLLEVNQGAIDLGSLQELTRWRDTFYDCIQKIEELEKQIDVARDVAEDTKSLGKELQNK 1310
Query: 632 IHEIHDLEEKCEELKSQ 582
I+E + E+K EEL+SQ
Sbjct: 1311 INEKNLAEQKVEELQSQ 1327
>SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1957
Score = 28.7 bits (61), Expect = 1.0
Identities = 13/35 (37%), Positives = 20/35 (57%)
Frame = -3
Query: 662 KNLEEAKAKKIHEIHDLEEKCEELKSQMSDLKAHL 558
KN +A+ IH LEE EE K ++++L + L
Sbjct: 1351 KNFLRKEAEMTENIHSLEEGKEETKKEIAELSSRL 1385
>SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 175
Score = 28.7 bits (61), Expect = 1.0
Identities = 12/32 (37%), Positives = 21/32 (65%)
Frame = -3
Query: 683 QNLEITLKNLEEAKAKKIHEIHDLEEKCEELK 588
QNL TLKN+E+A+ + I D+++ E++
Sbjct: 117 QNLSATLKNIEQARPTEEITIEDMKQAVPEIE 148
>SPCC63.11 |prp28||U5 snRNP-associated protein Prp28
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 27.9 bits (59), Expect = 1.8
Identities = 16/61 (26%), Positives = 34/61 (55%), Gaps = 1/61 (1%)
Frame = -3
Query: 782 QNDVKNLEEAVEELSLADDSEKIPYLIGEIFICQ-NLEITLKNLEEAKAKKIHEIHDLEE 606
Q+ + +LE+ V++ + ++ P + + + LE K +EEAKAK+ ++ DL +
Sbjct: 4 QDSIPSLEQLVQQKRVKEEKAARPKFLSKAERARLALERRQKEVEEAKAKQNDKLLDLRK 63
Query: 605 K 603
+
Sbjct: 64 R 64
>SPAC144.14 |klp8||kinesin-like protein Klp8|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 511
Score = 27.9 bits (59), Expect = 1.8
Identities = 16/28 (57%), Positives = 19/28 (67%), Gaps = 1/28 (3%)
Frame = -3
Query: 638 KKIHEI-HDLEEKCEELKSQMSDLKAHL 558
K I EI +D EE+ L+SQ S LKAHL
Sbjct: 402 KFIEEIRNDYEERIHALESQNSALKAHL 429
>SPBC365.05c |slu7||splicing factor Slu7|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 27.5 bits (58), Expect = 2.4
Identities = 15/34 (44%), Positives = 20/34 (58%), Gaps = 1/34 (2%)
Frame = -3
Query: 815 VDDYKDEL-KVKQNDVKNLEEAVEELSLADDSEK 717
+D Y D KVK KN E+ V +L++ D SEK
Sbjct: 325 LDRYGDGTSKVKDKKAKNNEKEVPDLAILDKSEK 358
>SPCC162.08c |nup211||nuclear pore complex associated
protein|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1837
Score = 27.5 bits (58), Expect = 2.4
Identities = 22/89 (24%), Positives = 42/89 (47%), Gaps = 2/89 (2%)
Frame = -3
Query: 818 KVDDYKDELKVKQNDVKNLEEAVEELSLADDS--EKIPYLIGEIFICQNLEITLKNLEEA 645
++++ K + + + + LE ++E + D+ +++ L E+ +NL+ K +E+A
Sbjct: 1353 QLEELKKNCEALEKEKQELETKLQETAKETDTFKQQVNSLNEEV---ENLK---KEVEQA 1406
Query: 644 KAKKIHEIHDLEEKCEELKSQMSDLKAHL 558
K EKCE LK AHL
Sbjct: 1407 NTKNTRLAAAWNEKCENLKKSSLTRFAHL 1435
>SPBC21D10.10 |||bromodomain protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 299
Score = 27.1 bits (57), Expect = 3.2
Identities = 23/82 (28%), Positives = 40/82 (48%), Gaps = 2/82 (2%)
Frame = -3
Query: 812 DDYKDELKVKQNDV-KNLEEAVEELSLADDSEKIPYLIGEIFICQN-LEITLKNLEEAKA 639
D K + K+ N V +N +A + L ++ + L EI C+ LE + + +AKA
Sbjct: 56 DSNKSDSKLLSNQVSQNDNDARKNLRKRLRNDVLKDLAEEIQGCEKKLESLYEEVAKAKA 115
Query: 638 KKIHEIHDLEEKCEELKSQMSD 573
K + + LEE +E K ++
Sbjct: 116 KAVEDQLALEEADKEAKKAKTE 137
>SPCC970.10c |brl2|rfp1|ubiquitin-protein ligase E3
Brl2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 680
Score = 26.2 bits (55), Expect = 5.6
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = -3
Query: 815 VDDYKDELKVKQNDVKNLEEAVEELSLA 732
++D ++ V QND K + A ELSL+
Sbjct: 343 INDLDHQISVMQNDEKRINNAKTELSLS 370
>SPAC27D7.02c |||GRIP domain protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 750
Score = 26.2 bits (55), Expect = 5.6
Identities = 21/81 (25%), Positives = 35/81 (43%)
Frame = -3
Query: 815 VDDYKDELKVKQNDVKNLEEAVEELSLADDSEKIPYLIGEIFICQNLEITLKNLEEAKAK 636
+D +L QN+V ++ ++E + I L G +NLE + +
Sbjct: 53 MDSVVQKLNELQNNVVAFQKLLQEKT---PLSSIQDLEGFREFMENLEHRYEMTVSEVRR 109
Query: 635 KIHEIHDLEEKCEELKSQMSD 573
HE++DL+ E LK Q D
Sbjct: 110 LSHEVNDLQTDRENLKHQFED 130
>SPBC29A3.13 |||PWWP domain protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 359
Score = 25.8 bits (54), Expect = 7.4
Identities = 12/42 (28%), Positives = 22/42 (52%)
Frame = -3
Query: 716 IPYLIGEIFICQNLEITLKNLEEAKAKKIHEIHDLEEKCEEL 591
IP+L E+ L LK + + + E++D+ +KC+ L
Sbjct: 301 IPFLNYELITSTKLAKVLKRIAFLEHLENDELYDIRQKCKNL 342
>SPAC1250.05 |rpl3002|rpl30-2, rpl30|60S ribosomal protein
L30|Schizosaccharomyces pombe|chr 1|||Manual
Length = 117
Score = 25.4 bits (53), Expect = 9.8
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +1
Query: 22 PKTFSHLQHNYISTRLAMYHYSSNNIFYYVLC 117
P S L++ + +R +++HYS NNI C
Sbjct: 64 PLRKSELEYYAMLSRCSVHHYSGNNIDLGTAC 95
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,880,942
Number of Sequences: 5004
Number of extensions: 54745
Number of successful extensions: 169
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 165
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 169
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 400438000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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