Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= rbmnc12i24
         (867 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC736.13 |||short chain dehydrogenase|Schizosaccharomyces pomb...    71   2e-13
SPAC19A8.06 |||short chain dehydrogenase|Schizosaccharomyces pom...    36   0.006
SPAC521.03 |||short chain dehydrogenase |Schizosaccharomyces pom...    33   0.053
SPCC24B10.20 |||short chain dehydrogenase |Schizosaccharomyces p...    30   0.37 
SPCC663.08c |||short chain dehydrogenase |Schizosaccharomyces po...    29   0.65 
SPCC663.06c |||short chain dehydrogenase |Schizosaccharomyces po...    29   1.1  
SPCC663.09c |||short chain dehydrogenase |Schizosaccharomyces po...    28   1.5  
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni...    27   4.6  
SPAC4G8.12c |||alpha-1,2-mannosyltransferase |Schizosaccharomyce...    27   4.6  
SPAC1952.13 |ned1||lipin|Schizosaccharomyces pombe|chr 1|||Manual      27   4.6  
SPAC3G9.02 |oar2||3-oxoacyl-[acyl-carrier-protein] reductase Oar...    27   4.6  
SPBC30D10.05c |||sepiapterin reductase |Schizosaccharomyces pomb...    26   8.0  
SPBC1703.14c |top1||DNA topoisomerase I|Schizosaccharomyces pomb...    26   8.0  

>SPCC736.13 |||short chain dehydrogenase|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 339

 Score = 70.9 bits (166), Expect = 2e-13
 Identities = 57/225 (25%), Positives = 95/225 (42%), Gaps = 12/225 (5%)
 Frame = -1

Query: 852 DLASTASIREFVARFKGEEPHLHVLVNNAGVMEPPKGVTKDGFETQLGVXXXXXXXXXXX 673
           DL    S+ +    F  +E  LH+LVNNAG+M PP  +TKDG+E Q+             
Sbjct: 101 DLLDFESVYQAAESFIAKEEKLHILVNNAGIMNPPFELTKDGYELQIQTNYLSHYLFTEL 160

Query: 672 XXXXLKAXXXXXXXXXXXSAH---------QRGRIHKHDLNMSE-NYDASAAYSQSKLAN 523
               L+              H             I+  DLN+        A Y QSK A 
Sbjct: 161 LLPTLRRTAEECRPGDVRIVHVASIAYLQAPYSGIYFPDLNLPHVLLGTFARYGQSKYAQ 220

Query: 522 LLFARELGRRLLGTGVATIAVDPGLVDTDITRHMSMMKSVTRFFIYPLFWPFMKKPEIGG 343
           +L++  L +RL   G+ ++++ PG++ T++TR+        +     +F   +  P  G 
Sbjct: 221 ILYSIALAKRLEKYGIYSVSLHPGVIRTELTRYSPTF--ALKLLEKSVFQYLLLDPIRGA 278

Query: 342 QTVVHAALDPKL--KDCAGDYYVDMKKAEPSQEAQDFELAQWLWR 214
            T ++AA  P++  +   G Y+  + +      A D    + L+R
Sbjct: 279 MTSLYAATSPEISKEHLNGAYFTAIAQRGILHRAHDDAFVEELYR 323


>SPAC19A8.06 |||short chain dehydrogenase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 397

 Score = 36.3 bits (80), Expect = 0.006
 Identities = 59/256 (23%), Positives = 105/256 (41%), Gaps = 26/256 (10%)
 Frame = -1

Query: 852 DLASTASIREFVARFKGEEP--HLHVLVNNAGVMEPP---KGVTKDGFETQLG---VXXX 697
           DL+S  S+R+F  ++    P   L ++V  +GV+ PP   +  T++G E Q     +   
Sbjct: 136 DLSSMLSVRKFATKWIDCTPIRRLDMIVLCSGVLLPPFMDRQTTEEGVELQWATNFLGPY 195

Query: 696 XXXXXXXXXXXXLKAXXXXXXXXXXXSAHQRGRIHKHDLNMSEN-YDASAAYS---QSKL 529
                                     S++  G I  +DL++S + Y   + +     +KL
Sbjct: 196 QLLRILRPVIYGQPGHREVRIVAATCSSYILGNIDFNDLDLSNHPYPRKSPWKVVGNAKL 255

Query: 528 ANLLFARELGRRL--------LGTGVATIAVDPGLVDTDITRHMSMMKSVTRFFIYPLFW 373
           A + +  +  ++         +   + TI  +PG+V T   R +     V   F+Y L W
Sbjct: 256 ALMTYLYDFQKKAEAHERPDKMPCNLHTIMANPGVVRTPGFRRVVSFGKVWGLFLYLLLW 315

Query: 372 PF----MKKPEIGGQTVVHAALDPKLKDCAGDYYV-DMKKAEPS-QEAQDFELAQWLWRV 211
           PF    +K    G Q+  HA   P+         V +    E S +E  D E A+ L + 
Sbjct: 316 PFWWLLLKGTIHGAQSFFHAICSPEFASITQPVLVNECSIVEYSRKEITDPEFAEKLIKA 375

Query: 210 SEAWTKLDEHKQAIAK 163
           ++A  ++DE ++   K
Sbjct: 376 ADA--QIDEVEKQYKK 389


>SPAC521.03 |||short chain dehydrogenase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 259

 Score = 33.1 bits (72), Expect = 0.053
 Identities = 15/47 (31%), Positives = 25/47 (53%)
 Frame = -1

Query: 570 ENYDASAAYSQSKLANLLFARELGRRLLGTGVATIAVDPGLVDTDIT 430
           E+Y   + Y  +K A   F   L +  + T +  + VDPGLV+T+ +
Sbjct: 150 ESYVGGSVYCSTKSALAQFTSALRKETIDTRIRIMEVDPGLVETEFS 196


>SPCC24B10.20 |||short chain dehydrogenase |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 254

 Score = 30.3 bits (65), Expect = 0.37
 Identities = 14/40 (35%), Positives = 23/40 (57%)
 Frame = -1

Query: 552 AAYSQSKLANLLFARELGRRLLGTGVATIAVDPGLVDTDI 433
           +AY QSK A     +E+   L   G   +++ PG+V+TD+
Sbjct: 157 SAYGQSKAALNYTMKEISFELEKDGFVVVSIHPGVVNTDM 196


>SPCC663.08c |||short chain dehydrogenase |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 253

 Score = 29.5 bits (63), Expect = 0.65
 Identities = 20/69 (28%), Positives = 30/69 (43%)
 Frame = -1

Query: 558 ASAAYSQSKLANLLFARELGRRLLGTGVATIAVDPGLVDTDITRHMSMMKSVTRFFIYPL 379
           + +AY QSK A     +E+   L   G   I++ PG V TD  + +    +  +  I  L
Sbjct: 154 SQSAYGQSKAALNYTMKEISFELQDEGFIVISIHPGAVRTDSAQEIVNQHAEKKPEILDL 213

Query: 378 FWPFMKKPE 352
           F      PE
Sbjct: 214 FAKQALTPE 222


>SPCC663.06c |||short chain dehydrogenase |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 253

 Score = 28.7 bits (61), Expect = 1.1
 Identities = 14/39 (35%), Positives = 20/39 (51%)
 Frame = -1

Query: 552 AAYSQSKLANLLFARELGRRLLGTGVATIAVDPGLVDTD 436
           + Y QSK A     +E+   L   G   I++ PG+V TD
Sbjct: 156 SGYGQSKAALNFTMKEISFELQDEGFIVISIHPGMVRTD 194


>SPCC663.09c |||short chain dehydrogenase |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 253

 Score = 28.3 bits (60), Expect = 1.5
 Identities = 15/40 (37%), Positives = 21/40 (52%)
 Frame = -1

Query: 552 AAYSQSKLANLLFARELGRRLLGTGVATIAVDPGLVDTDI 433
           +AY QSK A     +EL   L       I++ PG+V TD+
Sbjct: 156 SAYGQSKAAINFTMKELSVELADEHFTFISIHPGVVKTDM 195


>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
            Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 2199

 Score = 26.6 bits (56), Expect = 4.6
 Identities = 14/43 (32%), Positives = 22/43 (51%)
 Frame = +1

Query: 703  IDPELGLEAVLSDSLGWFHHPGVIDQHVKVWLLSFESGNELSD 831
            I+ +L  +  + D++G  H   V+   VK W     SGN L+D
Sbjct: 1734 INDDLEEKLGIHDNIGLTHSLPVLKALVKTWWNEAASGNNLAD 1776


>SPAC4G8.12c |||alpha-1,2-mannosyltransferase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 533

 Score = 26.6 bits (56), Expect = 4.6
 Identities = 11/34 (32%), Positives = 17/34 (50%)
 Frame = +3

Query: 108 TTTLNQSIYLNLPPNSVCLWRWLVYARPAWSTPR 209
           TT  N ++Y N P     ++ W +Y+ P W   R
Sbjct: 380 TTMENCNLYFN-PEMPTTIYFWKIYSAPTWMLAR 412


>SPAC1952.13 |ned1||lipin|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 656

 Score = 26.6 bits (56), Expect = 4.6
 Identities = 18/57 (31%), Positives = 29/57 (50%)
 Frame = -1

Query: 573 SENYDASAAYSQSKLANLLFARELGRRLLGTGVATIAVDPGLVDTDITRHMSMMKSV 403
           S+   +  A + + L  L  A+ELG+RL G  + T   D G V  D+T + S   ++
Sbjct: 183 SDRTPSMPATTLADLRLLQKAKELGKRLSGKELPTRVGDNGDVMLDMTGYKSSAANI 239


>SPAC3G9.02 |oar2||3-oxoacyl-[acyl-carrier-protein] reductase Oar2
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 236

 Score = 26.6 bits (56), Expect = 4.6
 Identities = 12/39 (30%), Positives = 19/39 (48%)
 Frame = -1

Query: 555 SAAYSQSKLANLLFARELGRRLLGTGVATIAVDPGLVDT 439
           ++ Y+ SK     F + L   +   G+   A+ PG VDT
Sbjct: 148 TSVYAASKAGLESFTKVLAAEVASKGIRVNAISPGYVDT 186


>SPBC30D10.05c |||sepiapterin reductase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 247

 Score = 25.8 bits (54), Expect = 8.0
 Identities = 16/42 (38%), Positives = 22/42 (52%)
 Frame = -1

Query: 564 YDASAAYSQSKLANLLFARELGRRLLGTGVATIAVDPGLVDT 439
           + A AAY  SK A  +    LG       + ++AV PG+VDT
Sbjct: 144 FPAWAAYCCSKAAINMLVMNLGSE--EPDIMSVAVRPGVVDT 183


>SPBC1703.14c |top1||DNA topoisomerase I|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 814

 Score = 25.8 bits (54), Expect = 8.0
 Identities = 28/127 (22%), Positives = 49/127 (38%)
 Frame = -1

Query: 612 HQRGRIHKHDLNMSENYDASAAYSQSKLANLLFARELGRRLLGTGVATIAVDPGLVDTDI 433
           HQR     HD+ M    +   A    ++        L  +L  +    +A + G+ D+ I
Sbjct: 605 HQRSVTKNHDVQMERFAERIKALQYQRMRLRKMMLNLEPKLAKSKPELLAKEEGITDSWI 664

Query: 432 TRHMSMMKSVTRFFIYPLFWPFMKKPEIGGQTVVHAALDPKLKDCAGDYYVDMKKAEPSQ 253
            +H   +  + +  I         K +   +    AA DPK      +  V +K A+  +
Sbjct: 665 VKHHETLYELEKEKI---------KKKFDRENEKLAAEDPKSVLPESELEVRLKAADELK 715

Query: 252 EAQDFEL 232
           +A D EL
Sbjct: 716 KALDAEL 722


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,318,860
Number of Sequences: 5004
Number of extensions: 65202
Number of successful extensions: 185
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 175
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 185
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 432473040
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)