Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= rbmnc12b20
(834 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 330 1e-91
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 330 1e-91
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 330 1e-91
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 102 7e-23
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 60 4e-10
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 58 2e-09
SPBC21D10.06c |map4||cell agglutination protein Map4|Schizosacch... 27 3.3
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 4.3
SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein... 26 7.6
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 330 bits (812), Expect = 1e-91
Identities = 151/192 (78%), Positives = 170/192 (88%)
Frame = -3
Query: 832 LQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVG 653
LQDVYKIGGIGTVPVGRVETGV+KPG IV FAPA +TTEVKSVEMHHE+L +PGDNVG
Sbjct: 248 LQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVG 307
Query: 652 FNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIA 473
FNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIA
Sbjct: 308 FNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIA 367
Query: 472 CKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDM 293
CKFAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDM
Sbjct: 368 CKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDM 427
Query: 292 RQTVAVGVIKAV 257
RQTVAVGVIKAV
Sbjct: 428 RQTVAVGVIKAV 439
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 330 bits (812), Expect = 1e-91
Identities = 151/192 (78%), Positives = 170/192 (88%)
Frame = -3
Query: 832 LQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVG 653
LQDVYKIGGIGTVPVGRVETGV+KPG IV FAPA +TTEVKSVEMHHE+L +PGDNVG
Sbjct: 248 LQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVG 307
Query: 652 FNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIA 473
FNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIA
Sbjct: 308 FNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIA 367
Query: 472 CKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDM 293
CKFAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDM
Sbjct: 368 CKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDM 427
Query: 292 RQTVAVGVIKAV 257
RQTVAVGVIKAV
Sbjct: 428 RQTVAVGVIKAV 439
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 330 bits (812), Expect = 1e-91
Identities = 151/192 (78%), Positives = 170/192 (88%)
Frame = -3
Query: 832 LQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVG 653
LQDVYKIGGIGTVPVGRVETGV+KPG IV FAPA +TTEVKSVEMHHE+L +PGDNVG
Sbjct: 248 LQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVG 307
Query: 652 FNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIA 473
FNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIA
Sbjct: 308 FNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIA 367
Query: 472 CKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDM 293
CKFAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDM
Sbjct: 368 CKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDM 427
Query: 292 RQTVAVGVIKAV 257
RQTVAVGVIKAV
Sbjct: 428 RQTVAVGVIKAV 439
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 102 bits (244), Expect = 7e-23
Identities = 60/190 (31%), Positives = 101/190 (53%), Gaps = 2/190 (1%)
Frame = -3
Query: 817 KIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSV-EMHHEALQEAVPGDNVGFNVK 641
K +GT+ G++E G +K + V+ P N T EV ++ + E + ++ GD V V+
Sbjct: 476 KYKDLGTILEGKIEAGSIKKNSNVLVMPINQTLEVTAIYDEADEEISSSICGDQVRLRVR 535
Query: 640 NVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFA 461
+++ GYV +KN P F AQ+ +L P ++ GY+ V+ HTA FA
Sbjct: 536 GDD-SDVQTGYVLTSTKN-PVHATTRFIAQIAILELPSILTTGYSCVMHIHTAVEEVSFA 593
Query: 460 EIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTV 281
++ K+D+ T + ++ P G I L P+C+E F+++ +GRF +RD TV
Sbjct: 594 KLLHKLDK-TNRKSKKPPMFATKGMKIIAELETQTPVCMERFEDYQYMGRFTLRDQGTTV 652
Query: 280 AVG-VIKAVN 254
AVG V+K ++
Sbjct: 653 AVGKVVKILD 662
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 60.1 bits (139), Expect = 4e-10
Identities = 49/191 (25%), Positives = 85/191 (44%), Gaps = 2/191 (1%)
Frame = -3
Query: 832 LQDVYKIGGIGTVPVGRVETGVLKPGTIV--VFAPANITTEVKSVEMHHEALQEAVPGDN 659
++DV+ I G GTV GRVE G LK G + V +++ T V +EM + L AV GDN
Sbjct: 257 IEDVFSISGRGTVVTGRVERGTLKKGAEIEIVGYGSHLKTTVTGIEMFKKQLDAAVAGDN 316
Query: 658 VGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAH 479
G ++++ ++L+RG + P F A +L + T +D +
Sbjct: 317 CGLLLRSIKREQLKRGMIVAQPGTVAPH--QKFKASFYILTK--EEGGRRTGFVDKYRPQ 372
Query: 478 IACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVR 299
+ + +++ ++ T + K + GD + P+ +E Q RF VR
Sbjct: 373 LYSRTSDVTVEL---THPDPNDSDKMVMPGDNVEMICTLIHPIVIEKGQ------RFTVR 423
Query: 298 DMRQTVAVGVI 266
+ TV ++
Sbjct: 424 EGGSTVGTALV 434
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 57.6 bits (133), Expect = 2e-09
Identities = 54/191 (28%), Positives = 86/191 (45%), Gaps = 1/191 (0%)
Frame = -3
Query: 832 LQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPANITTEVKSVEMHHEALQE-AVPGDNV 656
+ DVY+ TV GRVE G ++ ++ + VK+V + + AV GD V
Sbjct: 409 IDDVYRSPRSVTV-TGRVEAGNVQVNQVLYDVSSQEDAYVKNVIRNSDPSSTWAVAGDTV 467
Query: 655 GFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHI 476
+ ++ V +LR G + + +N P + F A++ + G I +G T VL H+
Sbjct: 468 TLQLADIEVNQLRPGDILSNYEN-PVRRVRSFVAEIQTFDIHGPILSGSTLVL-----HL 521
Query: 475 ACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRD 296
+ K+ K + + S K I L PLC+ +E P LGRF +R
Sbjct: 522 GRTVTSVSLKIVTVNNKRSR-HIASRKRALVRISFLDGLFPLCLA--EECPALGRFILRR 578
Query: 295 MRQTVAVGVIK 263
TVA G++K
Sbjct: 579 SGDTVAAGIVK 589
>SPBC21D10.06c |map4||cell agglutination protein
Map4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 948
Score = 27.1 bits (57), Expect = 3.3
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = +3
Query: 714 TSVVMLAGAKTTMVPGFNTPVSTLPTGTVPIPPILYTSC 830
+SVV+ + +T V + + VST TGTV +P +C
Sbjct: 86 SSVVLYSAKETVTVSSYWSLVSTSVTGTVYVPYTSSVAC 124
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 26.6 bits (56), Expect = 4.3
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = +2
Query: 620 QFLDGHVLYVETYIVSRYSFLESFV 694
+F DGH+L ETY+ LES V
Sbjct: 527 KFFDGHLLASETYVGGHVESLESGV 551
>SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 670
Score = 25.8 bits (54), Expect = 7.6
Identities = 24/85 (28%), Positives = 42/85 (49%), Gaps = 2/85 (2%)
Frame = +3
Query: 213 SAALVTLPPPASLKLTALMTPTATVCLMSRTAKRPRGGNSWKDSTH--RGLEGTKLTMAA 386
S +VTLPPPAS ++ T T T + S ++ G+ + +++ + ++++
Sbjct: 183 STDIVTLPPPAS-STSSFSTITNTSMIPSSSSFTTTTGSPYYNTSSFLPSSVISSASLSS 241
Query: 387 SPDLMDFGLTSVDLPVRRSTFSLIS 461
S L +TS PV S+ SL S
Sbjct: 242 SSVLPTSIITSTSTPVTVSSSSLSS 266
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,477,397
Number of Sequences: 5004
Number of extensions: 72678
Number of successful extensions: 228
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 218
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 225
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 410448950
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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