Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= rbmnc11m04
(822 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 80 3e-16
SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 74 2e-14
SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 73 4e-14
SPAPJ696.01c |vps17||retromer complex subunit Vps17|Schizosaccha... 31 0.20
SPAC664.13 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||... 30 0.46
SPBC13E7.10c |brf1|SPBC30D10.20|transcription factor TFIIIB comp... 28 1.8
SPCC1223.06 |tea1|alp8|cell end marker Tea1|Schizosaccharomyces ... 27 2.4
SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ... 27 3.2
SPAC4H3.14c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 27 4.3
SPCC825.01 |||ribosome biogenesis ATPase, Arb family |Schizosacc... 26 5.6
SPBC16C6.10 |chp2||chromodomain protein 2|Schizosaccharomyces po... 26 7.4
SPAC17G8.01c |trl1|SPAC6C3.10c|tRNA ligase Trl1 |Schizosaccharom... 26 7.4
SPAC2F3.10 |||GARP complex subunit Vps54 |Schizosaccharomyces po... 26 7.4
SPCPJ732.01 |vps5||retromer complex subunit Vps5|Schizosaccharom... 25 9.8
>SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr
1|||Manual
Length = 663
Score = 80.2 bits (189), Expect = 3e-16
Identities = 37/79 (46%), Positives = 54/79 (68%), Gaps = 1/79 (1%)
Frame = -3
Query: 766 RVESRNELESYAYSIKNQLQDKEKLGAKVTDDDKAKMEEALDAAIKWLE-DNQDAESEEY 590
R+E+RN LE+YAYS+K Q D E+LG KV +DK + +A++ +WLE +DA EE+
Sbjct: 563 RIEARNTLENYAYSLKGQFDDDEQLGGKVDPEDKQAVLDAVEDVAEWLEIHGEDASKEEF 622
Query: 589 KKQKKTLEDVVQPIIAKLY 533
+ Q++ L+ VV PI KLY
Sbjct: 623 EDQRQKLDAVVHPITQKLY 641
>SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 644
Score = 74.1 bits (174), Expect = 2e-14
Identities = 34/78 (43%), Positives = 52/78 (66%)
Frame = -3
Query: 766 RVESRNELESYAYSIKNQLQDKEKLGAKVTDDDKAKMEEALDAAIKWLEDNQDAESEEYK 587
R++++N LESYAYS++N L D L KV DK +++A+ I+WL+ N A +EY+
Sbjct: 533 RIQAKNHLESYAYSLRNSLDDPN-LKDKVDASDKEAIDKAVKETIEWLDHNTTAAKDEYE 591
Query: 586 KQKKTLEDVVQPIIAKLY 533
++K LE V PI+AK+Y
Sbjct: 592 DKQKELEGVANPIMAKIY 609
>SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 647
Score = 73.3 bits (172), Expect = 4e-14
Identities = 33/78 (42%), Positives = 52/78 (66%)
Frame = -3
Query: 766 RVESRNELESYAYSIKNQLQDKEKLGAKVTDDDKAKMEEALDAAIKWLEDNQDAESEEYK 587
R++++N LESYAYS++N L D L KV DK +++A+ I+WL+ N A +E++
Sbjct: 533 RIQAKNHLESYAYSLRNSLDDPN-LKDKVDASDKETVDKAVKETIEWLDSNTTAAKDEFE 591
Query: 586 KQKKTLEDVVQPIIAKLY 533
++K LE V PI+AK+Y
Sbjct: 592 AKQKELESVANPIMAKIY 609
>SPAPJ696.01c |vps17||retromer complex subunit
Vps17|Schizosaccharomyces pombe|chr 1|||Manual
Length = 549
Score = 31.1 bits (67), Expect = 0.20
Identities = 21/64 (32%), Positives = 30/64 (46%)
Frame = -3
Query: 763 VESRNELESYAYSIKNQLQDKEKLGAKVTDDDKAKMEEALDAAIKWLEDNQDAESEEYKK 584
+ S+N+ SY S N+LQD K+ +K DD + E K L D D + K
Sbjct: 355 ISSKNQTNSYL-SAANRLQDSPKI-SKARTDDALQALEVARVHEKLLSDKVDFVTLNLVK 412
Query: 583 QKKT 572
+ KT
Sbjct: 413 ESKT 416
>SPAC664.13 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 117
Score = 29.9 bits (64), Expect = 0.46
Identities = 13/48 (27%), Positives = 30/48 (62%)
Frame = -3
Query: 682 VTDDDKAKMEEALDAAIKWLEDNQDAESEEYKKQKKTLEDVVQPIIAK 539
V D+ + E+ L+ ++ ++ N+ ESE+YK +++L V + ++A+
Sbjct: 36 VVDEYSKESEDVLENGLEHVQSNEFTESEDYKSIERSLA-VTEAVMAR 82
>SPBC13E7.10c |brf1|SPBC30D10.20|transcription factor TFIIIB complex
subunit Brf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 500
Score = 27.9 bits (59), Expect = 1.8
Identities = 19/73 (26%), Positives = 34/73 (46%), Gaps = 4/73 (5%)
Frame = -3
Query: 751 NELESYAYSIKNQLQDKEKLGAKVTDDDKAKM----EEALDAAIKWLEDNQDAESEEYKK 584
+EL + + + ++E V DD+ + +E L W+E N+D +EE K
Sbjct: 374 DELRKISLQVNVKFSEEEVTLEDVDDDEIEDILLDKDEILTKTQVWMELNKDYLAEEEAK 433
Query: 583 QKKTLEDVVQPII 545
K ED+ + I+
Sbjct: 434 NLKLQEDLKKGIV 446
>SPCC1223.06 |tea1|alp8|cell end marker Tea1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1147
Score = 27.5 bits (58), Expect = 2.4
Identities = 13/30 (43%), Positives = 19/30 (63%), Gaps = 2/30 (6%)
Frame = -3
Query: 724 IKNQLQD--KEKLGAKVTDDDKAKMEEALD 641
+KNQ+ D KEKL +DD+K ++E D
Sbjct: 1094 LKNQIDDLAKEKLPLSSSDDEKVNIKEKTD 1123
>SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1044
Score = 27.1 bits (57), Expect = 3.2
Identities = 15/53 (28%), Positives = 26/53 (49%)
Frame = -3
Query: 712 LQDKEKLGAKVTDDDKAKMEEALDAAIKWLEDNQDAESEEYKKQKKTLEDVVQ 554
L K+K+ K ++ +EALD ++K ++ +E K K LE+ Q
Sbjct: 600 LSMKQKMQLKSLREEIDNTKEALDLSVKERSIQEEKLNESLKTSKTNLEEQTQ 652
>SPAC4H3.14c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 345
Score = 26.6 bits (56), Expect = 4.3
Identities = 16/56 (28%), Positives = 34/56 (60%), Gaps = 3/56 (5%)
Frame = -3
Query: 721 KNQLQDKEKLGAKVTDDDKAKMEEALDAAIKWLE---DNQDAESEEYKKQKKTLED 563
K + + +E+ ++TD + ++ L I+ LE + ++E+E+Y+KQK+ LE+
Sbjct: 135 KEEQKIQEEFERQITD--LLEEQQQLKLEIERLEAETERANSETEQYEKQKEALEE 188
>SPCC825.01 |||ribosome biogenesis ATPase, Arb family
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 822
Score = 26.2 bits (55), Expect = 5.6
Identities = 15/64 (23%), Positives = 30/64 (46%)
Frame = -3
Query: 766 RVESRNELESYAYSIKNQLQDKEKLGAKVTDDDKAKMEEALDAAIKWLEDNQDAESEEYK 587
+ +N ++ A + + D+E+ + K K ++ D + L+D SE+ K
Sbjct: 119 KASPKNAFDALADDMDDLSLDEEESESSEKSKKKKKKSKSKDDGSEALDDGDIESSEKDK 178
Query: 586 KQKK 575
K+KK
Sbjct: 179 KKKK 182
>SPBC16C6.10 |chp2||chromodomain protein 2|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 380
Score = 25.8 bits (54), Expect = 7.4
Identities = 16/69 (23%), Positives = 35/69 (50%), Gaps = 1/69 (1%)
Frame = -3
Query: 760 ESRNELESYAYSIKNQLQDKEKLGAKVTDD-DKAKMEEALDAAIKWLEDNQDAESEEYKK 584
ES N +S +Y + ++ V D ++ M+E K+++ ++ +++ +
Sbjct: 260 ESSNSDDSISYKRRRSRNAANRITDYVDSDLSESSMKEKQSKIEKYMKSDKSSKNFKPPF 319
Query: 583 QKKTLEDVV 557
QKK+ ED+V
Sbjct: 320 QKKSWEDLV 328
>SPAC17G8.01c |trl1|SPAC6C3.10c|tRNA ligase Trl1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 787
Score = 25.8 bits (54), Expect = 7.4
Identities = 14/45 (31%), Positives = 21/45 (46%)
Frame = +2
Query: 191 AQLTIAFTKNTKCTFFLQKQAHVSLTRACATADTRSDLDSVRFVA 325
A+ I +N F + H+S R+ D + +D VRFVA
Sbjct: 430 AKAIIEEFRNGHSVVFADRNNHISNMRSTLQTDILALIDGVRFVA 474
>SPAC2F3.10 |||GARP complex subunit Vps54 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 949
Score = 25.8 bits (54), Expect = 7.4
Identities = 16/52 (30%), Positives = 24/52 (46%), Gaps = 8/52 (15%)
Frame = -1
Query: 351 LYECS-----EWACATNLTESRSDRVSAVAHARVSETCAC---FCKKNVHFV 220
++ECS EW + + D V H +VSE C F K+++H V
Sbjct: 662 VFECSGNTPVEWTRLLSPEAEKEDENHRVHHVKVSEDCTATVSFGKQSLHIV 713
>SPCPJ732.01 |vps5||retromer complex subunit
Vps5|Schizosaccharomyces pombe|chr 3|||Manual
Length = 576
Score = 25.4 bits (53), Expect = 9.8
Identities = 12/41 (29%), Positives = 20/41 (48%)
Frame = +1
Query: 88 HTYRTLRSSHSVRE*SIRYRKTESTDVINLHVNKRTINYCF 210
HT + + SH+V S R + V N+ V +R ++ F
Sbjct: 210 HTVKEITKSHTVYSVSTRLEEHNQPSVSNVTVQRRYNDFAF 250
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,777,668
Number of Sequences: 5004
Number of extensions: 52159
Number of successful extensions: 156
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 150
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 153
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 402440190
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -