Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= rbmnc10o01
(854 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr... 360 e-100
SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr 3|... 161 1e-40
SPBC1198.10c |||asparagine-tRNA ligase Slm5|Schizosaccharomyces ... 42 1e-04
SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|ch... 42 1e-04
SPCC1223.07c |||aspartate-tRNA ligase |Schizosaccharomyces pombe... 36 0.006
SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyce... 34 0.030
SPCC1322.14c |vtc4||vacuolar transporter chaperone |Schizosaccha... 28 1.5
SPAC6F12.12 |par2|pbp2|protein phosphatase regulatory subunit Pa... 27 2.6
SPAC694.02 |||DEAD/DEAH box helicase|Schizosaccharomyces pombe|c... 27 4.5
SPAC56F8.07 |||dubious|Schizosaccharomyces pombe|chr 1|||Manual 26 5.9
SPBC16D10.05 |mok13||alpha-1,3-glucan synthase Mok13|Schizosacch... 26 5.9
SPBPB21E7.01c |eno102|eno1, SPBPB8B6.07c, eno1|enolase |Schizosa... 26 5.9
SPCPB1C11.02 |||amino acid permease, unknown 16|Schizosaccharomy... 26 7.8
SPAC17A5.15c |||glutamate-tRNA ligase |Schizosaccharomyces pombe... 26 7.8
>SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 591
Score = 360 bits (886), Expect = e-100
Identities = 164/244 (67%), Positives = 191/244 (78%)
Frame = -2
Query: 826 PEFTTCEFYMAYADYNDLMTITESILSGMVQSIHGSYKVKYHPEGPEGKEIEIDFTPPFA 647
PEFT+ EFY AYADY DLM TE +LSG+V+ + GSYKV YHPEGPEG + E+DF+ P+
Sbjct: 337 PEFTSIEFYQAYADYYDLMDTTEELLSGLVKDLTGSYKVPYHPEGPEGPKWELDFSRPWR 396
Query: 646 RVPMMATLERVLNVKLPAPDKLDTPEANDFLNNLCNTHQIECSPPRTTARLLDKLVSVFL 467
R+ M+ LE LN K P D+L TPEAN FL +LC H +EC+PP+T +RLLDKLV F+
Sbjct: 397 RINMIEYLEEKLNTKFPPGDQLHTPEANAFLRDLCAKHGVECAPPQTCSRLLDKLVGEFI 456
Query: 466 EEECINPTFILDHPQIMSPLSKYHRDVPGLTERFEVYVAKKEICNAYTELNDPATQRERF 287
E ECINPTFI+ HPQ+MSPL+KYHR GL ERFE +VA KEICNAYTELND QR RF
Sbjct: 457 ESECINPTFIIGHPQMMSPLAKYHRSDAGLCERFEAFVATKEICNAYTELNDIFDQRARF 516
Query: 286 EEQAKNRAAGDDETPPTDEAFCTALEYGLPPTGGWGLGVDRLTMFLTDSNNIKEVLLFPA 107
EEQA+ +A GDDE DE FCTALEYGLPPTGGWG+GVDRL MFLTDSN I+EVLLFP
Sbjct: 517 EEQARQKAQGDDEAQIIDENFCTALEYGLPPTGGWGMGVDRLVMFLTDSNTIREVLLFPH 576
Query: 106 MKPD 95
MKP+
Sbjct: 577 MKPE 580
>SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr
3|||Manual
Length = 531
Score = 161 bits (391), Expect = 1e-40
Identities = 92/249 (36%), Positives = 141/249 (56%), Gaps = 7/249 (2%)
Frame = -2
Query: 826 PEFTTCEFYMAYADYNDLMTITESILSGMVQSIHGSYKVKYHPEGPEGKEIEIDFTPPFA 647
PEFT+CE Y AY + + +TE ++ + +I+G+ ++ G+ ++++ F
Sbjct: 292 PEFTSCEAYCAYLNLEGMKKLTEELIRFICLTINGNLQIS-------GQTVDLE--KGFE 342
Query: 646 RVPMMATLERVLNVKLPAPDKLDTPEANDFLNNLCNTHQIECSPPRTTARLLDKLV-SVF 470
+ + L++ LNV+L D ++ L ++ +I T A LLDKL S+
Sbjct: 343 VIEFIPALQKELNVELSPLD--NSENCRKQLISIFKRCEIMLPKTCTVAHLLDKLFDSLV 400
Query: 469 LEEECINPTFILDHPQIMSPLSKYHRDVPGLTE-----RFEVYVAKKEICNAYTELNDPA 305
L+ +P F+++HP++MSPL+K + G E RFE+Y+ EICNAY E NDP
Sbjct: 401 LKYNTSSPKFVINHPEVMSPLAKSDIKLYGAVEQRISKRFELYIGGYEICNAYEEENDPV 460
Query: 304 TQRERFEEQAKNRAA-GDDETPPTDEAFCTALEYGLPPTGGWGLGVDRLTMFLTDSNNIK 128
Q +F+ Q +R GDDETP D F ALEYGLPPT GWG+GVDRL M +T + I
Sbjct: 461 AQYHKFQAQKYDRLQLGDDETPAPDSDFVHALEYGLPPTAGWGMGVDRLVMLMTGQSKIS 520
Query: 127 EVLLFPAMK 101
E+L F +++
Sbjct: 521 EILPFGSLR 529
>SPBC1198.10c |||asparagine-tRNA ligase Slm5|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 441
Score = 41.9 bits (94), Expect = 1e-04
Identities = 19/46 (41%), Positives = 28/46 (60%), Gaps = 1/46 (2%)
Frame = -2
Query: 244 PPTDEAFCTALEYGLPPTGGWGLGVDRLTMFLTDSN-NIKEVLLFP 110
PP + + ++Y P GG+GLG++RL FL N N+KE + FP
Sbjct: 388 PPELQWYLDLMKYSNAPHGGFGLGIERLIAFLEGENTNVKETIPFP 433
>SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|chr
3|||Manual
Length = 611
Score = 41.5 bits (93), Expect = 1e-04
Identities = 33/111 (29%), Positives = 48/111 (43%)
Frame = -2
Query: 442 FILDHPQIMSPLSKYHRDVPGLTERFEVYVAKKEICNAYTELNDPATQRERFEEQAKNRA 263
F H + L K V GL +++ V E+ +++P QR ++ K
Sbjct: 471 FTAPHWDDVHLLEKKPLSVRGL--HYDIVVNGIELGGGSIRIHNPDIQRFVLKDVLKL-- 526
Query: 262 AGDDETPPTDEAFCTALEYGLPPTGGWGLGVDRLTMFLTDSNNIKEVLLFP 110
+ T E L G PP GG LG DRL LT++ I+EV+ FP
Sbjct: 527 --PENRYATFEHLIRVLSSGCPPHGGIALGFDRLAALLTNAPGIREVIAFP 575
>SPCC1223.07c |||aspartate-tRNA ligase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 580
Score = 36.3 bits (80), Expect = 0.006
Identities = 22/92 (23%), Positives = 41/92 (44%)
Frame = -2
Query: 385 PGLTERFEVYVAKKEICNAYTELNDPATQRERFEEQAKNRAAGDDETPPTDEAFCTALEY 206
P + ++ ++ +EI + ++DP ER + + G + + A
Sbjct: 488 PRYSNSYDFFMKGQEIMSGAQRIHDPELLVERMKALGVSPDVGLQQ-------YIDAFAI 540
Query: 205 GLPPTGGWGLGVDRLTMFLTDSNNIKEVLLFP 110
G PP G G+G++R+ MF + NI+ FP
Sbjct: 541 GCPPHAGGGIGLERVVMFYLNLPNIRLASSFP 572
>SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 568
Score = 33.9 bits (74), Expect = 0.030
Identities = 15/39 (38%), Positives = 21/39 (53%)
Frame = -2
Query: 226 FCTALEYGLPPTGGWGLGVDRLTMFLTDSNNIKEVLLFP 110
F +YG GG GLG++R +L D ++E LFP
Sbjct: 522 FTEQRKYGTTEHGGCGLGLERFLAWLCDRYTVRECCLFP 560
>SPCC1322.14c |vtc4||vacuolar transporter chaperone
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 721
Score = 28.3 bits (60), Expect = 1.5
Identities = 19/63 (30%), Positives = 31/63 (49%), Gaps = 3/63 (4%)
Frame = -2
Query: 694 GPEGKEIEIDFTPPFARVP---MMATLERVLNVKLPAPDKLDTPEANDFLNNLCNTHQIE 524
G + ++I PF ++P ++ +L VKL D PE ++NNL N+H +E
Sbjct: 390 GNNWRRMDIGIDYPFDQLPDEDIVRFPYAILEVKLQTQFGQDPPE---WVNNLVNSHLVE 446
Query: 523 CSP 515
P
Sbjct: 447 AVP 449
>SPAC6F12.12 |par2|pbp2|protein phosphatase regulatory subunit
Par2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 627
Score = 27.5 bits (58), Expect = 2.6
Identities = 10/25 (40%), Positives = 17/25 (68%)
Frame = -2
Query: 376 TERFEVYVAKKEICNAYTELNDPAT 302
+ER E+++ K E CN + NDP++
Sbjct: 217 SEREELFIKKLEQCNIIFDFNDPSS 241
>SPAC694.02 |||DEAD/DEAH box helicase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1717
Score = 26.6 bits (56), Expect = 4.5
Identities = 24/95 (25%), Positives = 43/95 (45%), Gaps = 9/95 (9%)
Frame = -2
Query: 319 LNDPATQRERFEEQAKNRAAGD--------DETPPTDEAFCTALE-YGLPPTGGWGLGVD 167
+ND +RF++ + R GD ++ D+ F + ++ Y TG G +
Sbjct: 435 INDKTMTSKRFDDLSATRKTGDNTKDRKQANKVRRQDQFFISHIQKYASSLTGAKGGQLK 494
Query: 166 RLTMFLTDSNNIKEVLLFPAMKPDDPNKNNTEEEN 62
R T+F N+ KE A+K + KN+ + +N
Sbjct: 495 RQTIFTQGPNSTKESK--QAVKEETSVKNSPKGKN 527
>SPAC56F8.07 |||dubious|Schizosaccharomyces pombe|chr 1|||Manual
Length = 183
Score = 26.2 bits (55), Expect = 5.9
Identities = 14/43 (32%), Positives = 19/43 (44%), Gaps = 2/43 (4%)
Frame = +2
Query: 605 HVKNTFQCCHHR-YSSKGWCEIDFYFLTL-WSFWMIFDLVGSM 727
H T CC ++ K W + FYF L WM D+ G +
Sbjct: 121 HAFTTTWCCMFELFAEKKWMIMSFYFPYLAIPLWMAIDMGGRL 163
>SPBC16D10.05 |mok13||alpha-1,3-glucan synthase
Mok13|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2358
Score = 26.2 bits (55), Expect = 5.9
Identities = 15/33 (45%), Positives = 20/33 (60%)
Frame = -2
Query: 649 ARVPMMATLERVLNVKLPAPDKLDTPEANDFLN 551
AR P+ L++V KLP PD LDT + +D N
Sbjct: 1369 ARYPIFWGLKKV--GKLPNPDPLDTAQLDDPTN 1399
>SPBPB21E7.01c |eno102|eno1, SPBPB8B6.07c, eno1|enolase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 440
Score = 26.2 bits (55), Expect = 5.9
Identities = 11/29 (37%), Positives = 17/29 (58%)
Frame = +2
Query: 452 YTLLLQKYRNQFIQEPRGGPRWRALNLMS 538
Y+ L++KY FI++P W A + MS
Sbjct: 283 YSALIEKYPIVFIEDPFSEEDWGAFSYMS 311
>SPCPB1C11.02 |||amino acid permease, unknown 16|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 505
Score = 25.8 bits (54), Expect = 7.8
Identities = 13/37 (35%), Positives = 18/37 (48%)
Frame = +2
Query: 188 AGRRQAIF*SCTKRFIGWGSFIVTSSTILGLLFESFA 298
+G +F C K I W S + TS+T + L S A
Sbjct: 343 SGHAPKVFSKCNKHGIPWLSVLATSATAILCLMSSQA 379
>SPAC17A5.15c |||glutamate-tRNA ligase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 716
Score = 25.8 bits (54), Expect = 7.8
Identities = 11/31 (35%), Positives = 18/31 (58%)
Frame = +1
Query: 190 RSAAGHILELYKTLHRLGEFHRHQQHDSWLA 282
R A+G + L LH G+F + ++ +WLA
Sbjct: 592 RDASGKVTSLKLELHLDGDFKKTEKKVTWLA 622
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,844,853
Number of Sequences: 5004
Number of extensions: 86896
Number of successful extensions: 261
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 247
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 258
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 424464280
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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