Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= rbmnc10k16
         (850 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC16E8.15 |tif45|tif1|translation initiation factor eIF4E, 4F ...    83   4e-17
SPBC1709.18 |tif452|SPBC409.01|translation initiation factor eIF...    83   5e-17
SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein lig...    29   1.1  
SPAC20H4.04 |mfh2||ATP-dependent 3' to 5' DNA helicase |Schizosa...    29   1.1  
SPAC29E6.02 |prp3|SPAC30.06|U4/U6 x U5 tri-snRNP complex subunit...    27   4.4  
SPAC1805.10 |||sequence orphan|Schizosaccharomyces pombe|chr 1||...    26   7.8  

>SPAC16E8.15 |tif45|tif1|translation initiation factor eIF4E, 4F
           complex subunit |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 218

 Score = 83.4 bits (197), Expect = 4e-17
 Identities = 43/127 (33%), Positives = 64/127 (50%), Gaps = 3/127 (2%)
 Frame = -1

Query: 850 YAVFQQGIRPMWEDDANKMGGRWLISLEKKQRFTDLDRFWLDVVLLLIGENFE-NSDEIC 674
           Y+ F++G+RP WED  NK GG+W     K +    LD  WL  VL  IGE  +    E+ 
Sbjct: 93  YSFFREGVRPEWEDVHNKTGGKWAFQ-NKGRGGNALDEMWLTTVLAAIGETLDPTGQEVM 151

Query: 673 GAVVNVRPKVDKIAIWTADAMKQHATIEIGKKLKEQLGI--HGKIGFQVHRDTMVKHSSA 500
           G V+N+R    ++A+WT     +   +EIG + K+ L +     I F  H D+    S+ 
Sbjct: 152 GVVINMRKGFYRLAVWTKSCNNREVLMEIGTRFKQVLNLPRSETIEFSAHEDSSKSGSTR 211

Query: 499 TKNLYTV 479
            K   +V
Sbjct: 212 AKTRMSV 218


>SPBC1709.18 |tif452|SPBC409.01|translation initiation factor eIF4E
           4F complex subunit |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 243

 Score = 83.0 bits (196), Expect = 5e-17
 Identities = 45/122 (36%), Positives = 65/122 (53%), Gaps = 3/122 (2%)
 Frame = -1

Query: 850 YAVFQQGIRPMWEDDANKMGGRWLISLEKKQRFTDLDRFWLDVVLLLIGENFE-NSDEIC 674
           Y+ F +GIRP WED  N  GG+W  + + K + ++LD  WL +VL  IGE  +    E+ 
Sbjct: 119 YSYFLKGIRPEWEDPQNMNGGKW--AYQSKHKGSNLDELWLYMVLAAIGETLDPTGKEVT 176

Query: 673 GAVVNVRPKVDKIAIWTADAMKQHATIEIGKKLKEQLGIHGK--IGFQVHRDTMVKHSSA 500
           G V N+R    +IA+WT +   +    +IG + KE LGI  K  I +  H D+    S  
Sbjct: 177 GVVCNMRKGFYRIAVWTRNCNDKDVLEKIGLRFKEVLGISDKETIEYSAHEDSSKAGSMR 236

Query: 499 TK 494
            K
Sbjct: 237 AK 238


>SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein ligase
            E3 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1647

 Score = 28.7 bits (61), Expect = 1.1
 Identities = 21/83 (25%), Positives = 36/83 (43%), Gaps = 1/83 (1%)
 Frame = +3

Query: 147  LPTSRVAYLTTYNYVTLHTDTRA*V*YKIRLLTFYSSRGLYHVTM*DKYDYKN*TNTTSV 326
            +P ++  +   Y Y+ L  D      +K  +  FY      H  M  + +Y+N T   S+
Sbjct: 1092 VPRNKTIFRILYEYIQLSDDHTLDDFWKTPVPIFYGEPDCIHNDMKGELNYENETEGFSI 1151

Query: 327  KYNFIL-LL*LRKFSLWDVLNIF 392
                IL LL +  + + DV  +F
Sbjct: 1152 NIREILDLLSILYYGIRDVHTLF 1174


>SPAC20H4.04 |mfh2||ATP-dependent 3' to 5' DNA helicase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 783

 Score = 28.7 bits (61), Expect = 1.1
 Identities = 16/34 (47%), Positives = 21/34 (61%), Gaps = 2/34 (5%)
 Frame = -3

Query: 560 DTRQDW-FPSTQRH-NG*A*FCDQESIHCLVLAL 465
           D  Q+W FP TQ++ N    FC+Q   H L+LAL
Sbjct: 98  DAMQNWIFPQTQQYRNYQKEFCEQALFHNLLLAL 131


>SPAC29E6.02 |prp3|SPAC30.06|U4/U6 x U5 tri-snRNP complex subunit
           Prp3 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 542

 Score = 26.6 bits (56), Expect = 4.4
 Identities = 14/41 (34%), Positives = 20/41 (48%)
 Frame = +3

Query: 93  INMSVKHIIPVSSR*FHNLPTSRVAYLTTYNYVTLHTDTRA 215
           IN +++H IPV      N P+S   +LT      +   TRA
Sbjct: 271 INSAIQHPIPVLPPYAKNQPSSHSVFLTKKEQKKIRRQTRA 311


>SPAC1805.10 |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 527

 Score = 25.8 bits (54), Expect = 7.8
 Identities = 13/38 (34%), Positives = 20/38 (52%), Gaps = 1/38 (2%)
 Frame = -1

Query: 829 IRPMWEDDANKMGGRWLIS-LEKKQRFTDLDRFWLDVV 719
           +  +WE   N   G +L   +EKK  F  +DR+W  V+
Sbjct: 161 VAALWEQ-FNIFHGSYLFDEIEKKSSFEIIDRWWTSVL 197


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,212,938
Number of Sequences: 5004
Number of extensions: 62354
Number of successful extensions: 158
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 152
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 154
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 420459900
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)