BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= rbmmt16m16
(753 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC16E8.15 |tif45|tif1|translation initiation factor eIF4E, 4F ... 54 2e-08
SPBC1709.18 |tif452|SPBC409.01|translation initiation factor eIF... 54 3e-08
SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein lig... 29 0.94
SPAC20H4.04 |mfh2||ATP-dependent 3' to 5' DNA helicase |Schizosa... 29 0.94
>SPAC16E8.15 |tif45|tif1|translation initiation factor eIF4E, 4F
complex subunit |Schizosaccharomyces pombe|chr
1|||Manual
Length = 218
Score = 54.4 bits (125), Expect = 2e-08
Identities = 29/92 (31%), Positives = 44/92 (47%), Gaps = 3/92 (3%)
Frame = -3
Query: 736 LDRFWLDVVLLLIGENFE-NSDEICGAVVNVRPKVDKIAIWTADAMKQHATIEIGKKLKE 560
LD WL VL IGE + E+ G V+N+R ++A+WT + +EIG + K+
Sbjct: 127 LDEMWLTTVLAAIGETLDPTGQEVMGVVINMRKGFYRLAVWTKSCNNREVLMEIGTRFKQ 186
Query: 559 QLGI--HGKIGFQVHRDTMVKHSSATKNLYTV 470
L + I F H D+ S+ K +V
Sbjct: 187 VLNLPRSETIEFSAHEDSSKSGSTRAKTRMSV 218
>SPBC1709.18 |tif452|SPBC409.01|translation initiation factor eIF4E
4F complex subunit |Schizosaccharomyces pombe|chr
2|||Manual
Length = 243
Score = 53.6 bits (123), Expect = 3e-08
Identities = 31/89 (34%), Positives = 45/89 (50%), Gaps = 3/89 (3%)
Frame = -3
Query: 742 TDLDRFWLDVVLLLIGENFE-NSDEICGAVVNVRPKVDKIAIWTADAMKQHATIEIGKKL 566
++LD WL +VL IGE + E+ G V N+R +IA+WT + + +IG +
Sbjct: 150 SNLDELWLYMVLAAIGETLDPTGKEVTGVVCNMRKGFYRIAVWTRNCNDKDVLEKIGLRF 209
Query: 565 KEQLGIHGK--IGFQVHRDTMVKHSSATK 485
KE LGI K I + H D+ S K
Sbjct: 210 KEVLGISDKETIEYSAHEDSSKAGSMRAK 238
>SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein ligase
E3 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1647
Score = 28.7 bits (61), Expect = 0.94
Identities = 21/83 (25%), Positives = 36/83 (43%), Gaps = 1/83 (1%)
Frame = +3
Query: 138 LPTSRVAYLTTYNYVTLHTDTRA*V*YKIRLLTFYSSRGLYHVTM*DKYDYKN*TNTTSV 317
+P ++ + Y Y+ L D +K + FY H M + +Y+N T S+
Sbjct: 1092 VPRNKTIFRILYEYIQLSDDHTLDDFWKTPVPIFYGEPDCIHNDMKGELNYENETEGFSI 1151
Query: 318 KYNFIL-LL*LRKFSLWDVLNIF 383
IL LL + + + DV +F
Sbjct: 1152 NIREILDLLSILYYGIRDVHTLF 1174
>SPAC20H4.04 |mfh2||ATP-dependent 3' to 5' DNA helicase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 783
Score = 28.7 bits (61), Expect = 0.94
Identities = 16/34 (47%), Positives = 21/34 (61%), Gaps = 2/34 (5%)
Frame = -2
Query: 551 DTRQDW-FPSTQRH-NG*A*FCDQESIHCLVLAL 456
D Q+W FP TQ++ N FC+Q H L+LAL
Sbjct: 98 DAMQNWIFPQTQQYRNYQKEFCEQALFHNLLLAL 131
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,722,093
Number of Sequences: 5004
Number of extensions: 50838
Number of successful extensions: 118
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 114
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 116
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 359287726
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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