BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= rbmmt16k12
(899 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4H3.10c |pyk1||pyruvate kinase |Schizosaccharomyces pombe|ch... 35 0.014
SPAC17A2.09c |csx1||RNA-binding protein Csx1|Schizosaccharomyces... 29 1.2
SPBC1289.15 ||SPBC8E4.07c|glycoprotein |Schizosaccharomyces pomb... 28 1.6
SPBC649.05 |cut12|stf1|spindle pole body protein Cut12 |Schizosa... 28 2.1
SPAC1B9.02c |sck1||serine/threonine protein kinase Sck1|Schizosa... 27 3.6
SPAC105.03c |||transcription factor |Schizosaccharomyces pombe|c... 27 4.8
SPBC29A3.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 27 4.8
SPBC23E6.01c ||SPBPJ758.01|RNA-binding protein, rrm type|Schizos... 26 6.3
SPCC18B5.03 |wee1||dual specificity protein kinase Wee1|Schizosa... 26 6.3
SPAC13A11.06 ||SPAC3H8.01|pyruvate decarboxylase |Schizosaccharo... 26 6.3
SPBC36B7.03 |sec63||ER protein translocation subcomplex subunit ... 26 8.4
SPAC23G3.03 |sib2||ornithine N5 monooxygenase |Schizosaccharomyc... 26 8.4
SPAC24C9.05c |mug70||conserved protein |Schizosaccharomyces pomb... 26 8.4
SPAC1F8.07c |||pyruvate decarboxylase |Schizosaccharomyces pombe... 26 8.4
>SPAC4H3.10c |pyk1||pyruvate kinase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 509
Score = 35.1 bits (77), Expect = 0.014
Identities = 19/45 (42%), Positives = 24/45 (53%)
Frame = -2
Query: 880 DVKVLVVGNPANTNALICSKYAPSIPKENFTAMTRLDQNRAQSQL 746
D K +VV + + A +CSKY PSIP +TR Q QS L
Sbjct: 402 DAKAIVVLSTSGNTARLCSKYRPSIP---IVMVTRCPQRARQSHL 443
>SPAC17A2.09c |csx1||RNA-binding protein Csx1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 632
Score = 28.7 bits (61), Expect = 1.2
Identities = 18/54 (33%), Positives = 28/54 (51%)
Frame = +3
Query: 192 CAYLM*VVFYTNYYP*RLLSQSLQTSKASCFSSTNSLPVASSI*RAKSEIVRPS 353
C Y +F T++ P L S TSK S +S + P++ + KSE+V P+
Sbjct: 5 CLYRRSSLFDTSFVP--LHSSIPATSKMSASNSDVNAPISPVVDEGKSELVSPT 56
>SPBC1289.15 ||SPBC8E4.07c|glycoprotein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1283
Score = 28.3 bits (60), Expect = 1.6
Identities = 13/42 (30%), Positives = 27/42 (64%)
Frame = +3
Query: 282 FSSTNSLPVASSI*RAKSEIVRPSTIFHFPLVTVTGNEYTTS 407
+S+TNSLP+ +I +E++ P+T ++ T+ + YT++
Sbjct: 130 YSNTNSLPITDTI-NGTTELIIPTTSYNNQSHTLIYSTYTST 170
>SPBC649.05 |cut12|stf1|spindle pole body protein Cut12
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 548
Score = 27.9 bits (59), Expect = 2.1
Identities = 13/35 (37%), Positives = 21/35 (60%)
Frame = +2
Query: 5 NEQNDMKYLTIES*CFKNIYNSNTKIKTLIKALTI 109
N ND L + CF+++ N+N K+K +IK + I
Sbjct: 197 NNNNDWSSLMKD--CFRDVVNNNRKMKEIIKDVMI 229
>SPAC1B9.02c |sck1||serine/threonine protein kinase
Sck1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 696
Score = 27.1 bits (57), Expect = 3.6
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = +2
Query: 407 TRSTIRSI*NNSHANPSVFSAKEPISHVV 493
T S++ SI NSH NP A +P++ V
Sbjct: 663 THSSVNSI--NSHGNPRTVDANDPVADTV 689
>SPAC105.03c |||transcription factor |Schizosaccharomyces pombe|chr
1|||Manual
Length = 708
Score = 26.6 bits (56), Expect = 4.8
Identities = 11/30 (36%), Positives = 17/30 (56%)
Frame = +2
Query: 743 SKLGLSPVLIETSHGSEIFFWNRWSILRTY 832
+K LSP+L+ + S I +W+ LR Y
Sbjct: 337 AKHKLSPILLTAMYASSIIYWDADKELRNY 366
>SPBC29A3.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 398
Score = 26.6 bits (56), Expect = 4.8
Identities = 10/21 (47%), Positives = 14/21 (66%)
Frame = -1
Query: 587 RRICNNGSKTWCCRYCCQENV 525
R++ NGS+ + C YC ENV
Sbjct: 369 RQLSRNGSQRFKCPYCPNENV 389
>SPBC23E6.01c ||SPBPJ758.01|RNA-binding protein, rrm
type|Schizosaccharomyces pombe|chr 2|||Manual
Length = 473
Score = 26.2 bits (55), Expect = 6.3
Identities = 11/21 (52%), Positives = 13/21 (61%)
Frame = -2
Query: 676 QFPDASNAVAIVGGAQKSVSE 614
QF D +N+ VGG K VSE
Sbjct: 296 QFADTANSTVFVGGLSKFVSE 316
>SPCC18B5.03 |wee1||dual specificity protein kinase
Wee1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 877
Score = 26.2 bits (55), Expect = 6.3
Identities = 23/74 (31%), Positives = 26/74 (35%), Gaps = 9/74 (12%)
Frame = -2
Query: 853 PANTNALICSKYAPSIPKENFTAMTRLDQNRAQSQL---------AAKIGVPVKDVKRVI 701
P N+N S SIP T N AQS L A K+G K +
Sbjct: 240 PPNSNNANPSTLFSSIPSSRHTTSNHFPSNSAQSSLFSPTARPLTARKLGFASSQTKSAV 299
Query: 700 IWGNHSSTQFPDAS 659
NHS DAS
Sbjct: 300 S-NNHSRNSSKDAS 312
>SPAC13A11.06 ||SPAC3H8.01|pyruvate decarboxylase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 571
Score = 26.2 bits (55), Expect = 6.3
Identities = 8/22 (36%), Positives = 15/22 (68%)
Frame = +3
Query: 804 GIDGAYLEHIRAFVLAGLPTTR 869
G+ G+Y EH+ + G+P+T+
Sbjct: 87 GVAGSYAEHVPVVHIVGMPSTK 108
>SPBC36B7.03 |sec63||ER protein translocation subcomplex subunit
Sec63 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 611
Score = 25.8 bits (54), Expect = 8.4
Identities = 10/13 (76%), Positives = 11/13 (84%)
Frame = +3
Query: 678 VDEWFPQMITLLT 716
VDEWFP+M T LT
Sbjct: 231 VDEWFPKMETSLT 243
>SPAC23G3.03 |sib2||ornithine N5 monooxygenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 431
Score = 25.8 bits (54), Expect = 8.4
Identities = 15/37 (40%), Positives = 18/37 (48%)
Frame = -3
Query: 402 LYIHSLLLLPMESGKLLRVSLSLILPVKCSMLLAKSW 292
L +HS + S KLLR S IL V C A+ W
Sbjct: 174 LIVHSSKFMSDHSQKLLRNSKHGILVVGCGQSAAEIW 210
>SPAC24C9.05c |mug70||conserved protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 730
Score = 25.8 bits (54), Expect = 8.4
Identities = 16/52 (30%), Positives = 26/52 (50%), Gaps = 3/52 (5%)
Frame = -2
Query: 748 LAAKIGVPVKDVKRVIIWGNHSSTQFPDASNAVAIVGGA---QKSVSEIIND 602
LA + G+P +V+ V + NH + P S + +G A +K V+ I D
Sbjct: 639 LARRRGLPRLEVRGVAAFTNHLESSHPPISTVDSSIGSASVVEKGVANSIVD 690
>SPAC1F8.07c |||pyruvate decarboxylase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 594
Score = 25.8 bits (54), Expect = 8.4
Identities = 10/26 (38%), Positives = 16/26 (61%)
Frame = +3
Query: 804 GIDGAYLEHIRAFVLAGLPTTRTFTS 881
GI GAY E++ +++G P T +S
Sbjct: 89 GIGGAYAENLPVILVSGSPNTNDLSS 114
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,684,410
Number of Sequences: 5004
Number of extensions: 78915
Number of successful extensions: 178
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 170
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 178
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 454497130
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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