BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= rbmmt14f23
(729 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1604.21c |ptr3|uba1, SPBC211.09|ubiquitin activating enzyme ... 146 2e-36
SPBPB2B2.02 |mug180||esterase/lipase |Schizosaccharomyces pombe|... 31 0.13
SPAC17G6.08 |pep7|vac1|prevacuole/endosomal FYVE tethering compo... 28 1.2
SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Sch... 27 3.6
SPAC26H5.11 |||spore wall assembly protein |Schizosaccharomyces ... 27 3.6
SPBC11B10.09 |cdc2|swo2|cyclin-dependent protein kinase Cdc2|Sch... 26 6.3
>SPBC1604.21c |ptr3|uba1, SPBC211.09|ubiquitin activating enzyme
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1012
Score = 146 bits (355), Expect = 2e-36
Identities = 71/144 (49%), Positives = 97/144 (67%), Gaps = 1/144 (0%)
Frame = -3
Query: 727 VCLELYKLAQGFNKLEVFKNGFVNLALPFFGFSEPIA-PSTNTYNENKWTLWDRFEVKGE 551
VCLEL KL G K+E +KNGF NLA+ F FS+PIA P + +WDR+ + +
Sbjct: 866 VCLELVKLVDGKKKIEEYKNGFFNLAIGLFTFSDPIASPKMKVNGKEIDKIWDRYNLP-D 924
Query: 550 ITLQQFLDHFKNEHKLEITMLSQGVCMLYSFFMPKAKRLERLNLPMSEVVTKVSKKKLEP 371
TLQ+ +D+F+ E LE+TMLS GV +LY+ F P K ERL L +SE+V +++KKKLEP
Sbjct: 925 CTLQELIDYFQKEEGLEVTMLSSGVSLLYANFQPPKKLAERLPLKISELVEQITKKKLEP 984
Query: 370 HVNALVFELCCNDDDGNDVEVPYV 299
LV E+CC+D +G DVEVP++
Sbjct: 985 FRKHLVLEICCDDANGEDVEVPFI 1008
>SPBPB2B2.02 |mug180||esterase/lipase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 381
Score = 31.5 bits (68), Expect = 0.13
Identities = 20/57 (35%), Positives = 28/57 (49%)
Frame = +3
Query: 291 VYFTYGTSTSFPSSSLQHNSKTNAFTCGSNFFLDTFVTTSDIGRFNRSSRLALGMKN 461
+YFT GT+ FP L+HN + F C S L +T D+ F S + +KN
Sbjct: 23 LYFTIGTN--FPHDELRHNLLSTLF-CSSMLHLSKGLTVKDVRIFFHDSIGSTLLKN 76
>SPAC17G6.08 |pep7|vac1|prevacuole/endosomal FYVE tethering
component Pep7 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 536
Score = 28.3 bits (60), Expect = 1.2
Identities = 16/37 (43%), Positives = 21/37 (56%)
Frame = -3
Query: 538 QFLDHFKNEHKLEITMLSQGVCMLYSFFMPKAKRLER 428
+FL K KLE S+ V ML + + KAKRLE+
Sbjct: 231 EFLRLSKRMKKLEELWTSENVSMLDALLLNKAKRLEQ 267
>SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1133
Score = 26.6 bits (56), Expect = 3.6
Identities = 18/47 (38%), Positives = 24/47 (51%), Gaps = 2/47 (4%)
Frame = -1
Query: 147 LQSKFQIITHSRFVSFVCSLSNDDVLHFYY-YFTS*KSFL-PNSIFY 13
L S+F+ S F F S+DD HFY +T + L PNS+ Y
Sbjct: 475 LWSRFRFPKDSEFPEFFKCSSDDDNTHFYVNLYTGETTMLFPNSMPY 521
>SPAC26H5.11 |||spore wall assembly protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 965
Score = 26.6 bits (56), Expect = 3.6
Identities = 12/29 (41%), Positives = 19/29 (65%)
Frame = +3
Query: 390 DTFVTTSDIGRFNRSSRLALGMKNE*SMH 476
+TFVTT DI + +R+S L+L + +H
Sbjct: 437 ETFVTTDDITQLSRTSTLSLSTASPRLVH 465
>SPBC11B10.09 |cdc2|swo2|cyclin-dependent protein kinase
Cdc2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 297
Score = 25.8 bits (54), Expect = 6.3
Identities = 14/34 (41%), Positives = 20/34 (58%)
Frame = -2
Query: 542 TAIFRSLQKRTQVGDHHVVSRCVHALFVLHAQSQ 441
TAI R + +V D + S CV L +LHA+S+
Sbjct: 47 TAI-REISLLKEVNDENNRSNCVRLLDILHAESK 79
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,864,351
Number of Sequences: 5004
Number of extensions: 59630
Number of successful extensions: 188
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 180
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 187
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 343230174
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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