Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= rbmmt13f04
(692 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 235 6e-63
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 117 1e-27
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 102 6e-23
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 100 2e-22
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 95 9e-21
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 71 2e-13
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 68 1e-12
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 68 1e-12
SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 44 2e-05
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 28 1.5
SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccha... 26 5.9
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 235 bits (574), Expect = 6e-63
Identities = 107/123 (86%), Positives = 114/123 (92%)
Frame = -2
Query: 598 KRFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADR 419
+RFR PEALFQPS LG+E GIHE TYNSIMKCDVDIRKDLY N V+SGGTTMYPGIADR
Sbjct: 253 ERFRAPEALFQPSALGLENAGIHEATYNSIMKCDVDIRKDLYGNVVMSGGTTMYPGIADR 312
Query: 418 MQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHR 239
MQKEI ALAPS+MK+KI+APPERKYSVWIGGSILASLSTFQQMWISKQEYDESGP IV+R
Sbjct: 313 MQKEIQALAPSSMKVKIVAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPGIVYR 372
Query: 238 KCF 230
KCF
Sbjct: 373 KCF 375
Score = 41.9 bits (94), Expect = 8e-05
Identities = 19/24 (79%), Positives = 20/24 (83%)
Frame = -3
Query: 666 ASSSXLEKSYELPDGQVITIGKRK 595
A SS LEKSYELPDGQVITIG +
Sbjct: 231 AQSSSLEKSYELPDGQVITIGNER 254
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 117 bits (282), Expect = 1e-27
Identities = 53/124 (42%), Positives = 83/124 (66%)
Frame = -2
Query: 601 TKRFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIAD 422
T+ F PE LF P +G EA G+H + SI+ D+D+R LY+N VLSGG+T+ G +
Sbjct: 257 TECFSAPEVLFNPEMMGSEASGLHIQLFKSILLSDIDLRSTLYSNIVLSGGSTLLRGFGE 316
Query: 421 RMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVH 242
R E+ A++ ++KI A PER ++ W+GGSILASLSTF+++ I+ +EY ++ +++
Sbjct: 317 RFISELRAISGKKNQVKIYASPERMHNAWLGGSILASLSTFRRLLITSEEY-KNDQNVIF 375
Query: 241 RKCF 230
R+ F
Sbjct: 376 RRRF 379
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 102 bits (244), Expect = 6e-23
Identities = 54/130 (41%), Positives = 74/130 (56%), Gaps = 11/130 (8%)
Frame = -2
Query: 613 HHRKTKRFRCPEALFQPSFLGMEAC---------GIHETTYNSIMKCDVDIRKDLYANTV 461
H +RFR E LF PSF + G+HE Y SI+ CD ++R L N V
Sbjct: 289 HKFGQERFRISEILFNPSFSASRSAETTPPQGSVGLHELVYQSILACDSELRSPLLNNIV 348
Query: 460 LSGGTTMYPGIADRMQKEITALAP-STMKIKII-APPERKYSVWIGGSILASLSTFQQMW 287
++GGT++ PG+++R+Q E+ LA S + + +VW GGSILASL FQ +W
Sbjct: 349 VTGGTSLIPGLSERLQAEVQRLATGSRINVHTAETASATSNAVWFGGSILASLDNFQHLW 408
Query: 286 ISKQEYDESG 257
+SKQEYDE G
Sbjct: 409 VSKQEYDEVG 418
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 100 bits (240), Expect = 2e-22
Identities = 53/127 (41%), Positives = 80/127 (62%), Gaps = 12/127 (9%)
Frame = -2
Query: 601 TKRFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIAD 422
++R+ CPE LFQP +G E G+ E +++I DVDIRK LY VLSGG++MY G+
Sbjct: 252 SERYECPECLFQPHLVGSEQPGLSEFIFDTIQAADVDIRKYLYRAIVLSGGSSMYAGLPS 311
Query: 421 RMQKEITAL--------APSTM---KIKIIAPPERKYSVWIGGSILAS-LSTFQQMWISK 278
R++KEI L P+ + K+KI P R+++V+IGG++LA ++ MW+SK
Sbjct: 312 RLEKEIKQLWFERVLHGDPARLPNFKVKIEDAPRRRHAVFIGGAVLADIMAQNDHMWVSK 371
Query: 277 QEYDESG 257
E++E G
Sbjct: 372 AEWEEYG 378
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 95.1 bits (226), Expect = 9e-21
Identities = 47/108 (43%), Positives = 71/108 (65%), Gaps = 6/108 (5%)
Frame = -2
Query: 538 GIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAP 359
GI + N I +CDVDIR L N ++ GGT++ G + R+Q E++ L P + ++KI A
Sbjct: 326 GIPQLFQNCISECDVDIRASLLNNVIVCGGTSLMQGFSLRLQNELSKLYPGS-RLKIHAS 384
Query: 358 P---ERKYSVWIGGSILASLSTFQQMWISKQEYDESGP---SIVHRKC 233
ER Y+ W+GGSIL+SL TF Q+WIS+QEY+E G +++ ++C
Sbjct: 385 GHVVERSYASWLGGSILSSLGTFHQLWISRQEYEEHGSDRLALIEKRC 432
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 70.9 bits (166), Expect = 2e-13
Identities = 31/122 (25%), Positives = 63/122 (51%)
Frame = -2
Query: 610 HRKTKRFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPG 431
H ++ R PE +F PS +G++ GI E + + + ++ ++ L +N +++GG PG
Sbjct: 590 HLNVEQIRVPEVIFSPSIVGIDQAGILEIMRSILQRHSLEEQQKLVSNVLITGGLGSLPG 649
Query: 430 IADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPS 251
+ R+++E+T++ P I + W G S + F+ ++++EY E GP
Sbjct: 650 METRIKRELTSIMPVGSSINVFRASNPLLDAWKGASEWSVTEKFKAAKVTREEYLEKGPE 709
Query: 250 IV 245
+
Sbjct: 710 YI 711
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 67.7 bits (158), Expect = 1e-12
Identities = 38/121 (31%), Positives = 59/121 (48%)
Frame = -2
Query: 598 KRFRCPEALFQPSFLGMEACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADR 419
+RF PE LF PS + + GI E S+ +I+ L N V GG +PG R
Sbjct: 279 ERFMIPELLFSPSDIEIREAGIPEAVMQSVTHFPENIQAMLLENVVTIGGNCKFPGFHKR 338
Query: 418 MQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHR 239
+ E+ +LAP+ ++K+ P + W S + L + I++ EY E G +I+ R
Sbjct: 339 LSSELRSLAPANWEVKVFEPSDPICFPWKKASHM-PLEHWNANKITRSEYSEHGANIMTR 397
Query: 238 K 236
K
Sbjct: 398 K 398
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 67.7 bits (158), Expect = 1e-12
Identities = 38/135 (28%), Positives = 64/135 (47%), Gaps = 15/135 (11%)
Frame = -2
Query: 598 KRFRCPEALFQPSFLGME-ACGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIAD 422
+RF PE F P + + E N + +D+RK LY N VLSGG+T++ +
Sbjct: 285 ERFLAPEIFFNPEIASSDFLTPLPELVDNVVQSSPIDVRKGLYKNIVLSGGSTLFKNFGN 344
Query: 421 RMQKEITALAPST--------------MKIKIIAPPERKYSVWIGGSILASLSTFQQMWI 284
R+Q+++ + + + +I+ ++ +VW GGS+LA F
Sbjct: 345 RLQRDLKRIVDERIHRSEMLSGAKSGGVDVNVISHKRQRNAVWFGGSLLAQTPEFGSYCH 404
Query: 283 SKQEYDESGPSIVHR 239
+K +Y+E G SI R
Sbjct: 405 TKADYEEYGASIARR 419
>SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 44.4 bits (100), Expect = 2e-05
Identities = 20/86 (23%), Positives = 42/86 (48%), Gaps = 3/86 (3%)
Frame = -2
Query: 484 KDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPP---ERKYSVWIGGSILA 314
K+LY++ ++ GG +PG A +++ I + + I +I PP + ++ W G I
Sbjct: 527 KNLYSSILIVGGAGQFPGFAHLLEERIHSKRANIPTISVIPPPRSMDAQFVAWKGACIYN 586
Query: 313 SLSTFQQMWISKQEYDESGPSIVHRK 236
+ ++WI ++ G ++ K
Sbjct: 587 RIRIVSELWIKNSDWKMLGSRVLQYK 612
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 27.9 bits (59), Expect = 1.5
Identities = 12/37 (32%), Positives = 24/37 (64%), Gaps = 3/37 (8%)
Frame = -2
Query: 337 WIGGSILASLSTFQQM---WISKQEYDESGPSIVHRK 236
++GGSI+A S + + +++ +EY + GP+ +H K
Sbjct: 486 FLGGSIVAKTSFNESVSSHYVTLEEYAQHGPTAIHTK 522
>SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 617
Score = 25.8 bits (54), Expect = 5.9
Identities = 13/37 (35%), Positives = 20/37 (54%)
Frame = -2
Query: 412 KEITALAPSTMKIKIIAPPERKYSVWIGGSILASLST 302
KEI++ A S + + + P E+ W ILA L+T
Sbjct: 212 KEISSQARSNIALPTLRPQEKNLIDWRDRKILAPLTT 248
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,895,253
Number of Sequences: 5004
Number of extensions: 60467
Number of successful extensions: 204
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 187
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 198
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 321951680
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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